Maize plants with improved disease resistance

ABSTRACT

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding corn plants containing one or more markers that are associated with resistance to fungi. The invention further includes germplasm and the use of germplasm containing at least one marker associated with resistance to  Fusarium  stalk rot (FSR) infection for introgression into elite germplasm in a breeding program, thus producing novel FSR resistant germplasm.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application No.61/847,153, filed Jul. 17, 2013, incorporated herein by reference in itsentirety.

INCORPORATION OF SEQUENCE LISTING

A sequence listing containing the file named “MONS364US_ST25.txt” whichis 51,332 bytes (measured in MS-Windows®) and created on Jun. 25, 2014comprises 165 nucleotide sequences, and is incorporated herein byreference in its entirety.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and morespecifically to methods and compositions for producing corn plantsexhibiting improved disease resistance.

BACKGROUND

Stalk rot infection reduces the efficiency of carbohydrate transportfrom the stalk up to the ears during grainfill, which reduces cropyield. A corn plant will die altogether if infection advances to thepoint that the pith pulls away from the outer rind of the stalk, whichcan eventually result in a stalk consisting of little more than a hollowtube that is no longer able transport water and nutrients to the rest ofthe plant. Furthermore, a stalk weakened by infection is more likely tocollapse at one or more points along its length (lodging), whichtypically results in a plant that yields no harvestable grain. Stalkrots typically reduce yields up to 5% in almost any field where corn iscultivated. In years with particularly bad infection rates, yield lossesreach 10-20%, and in some locations when infection is particularlyacute, 100% yield loss can occur.

One of the most common forms of stalk rot is Fusarium stalk rot, causedby several species of fungi, including Fusarium verticilliodes e J.Sheld. (sexual stage: G. moniliformis Sawada) Ito in Ito & Kimura,formerly Fusarium moniliforme, telemorph Gibberella fujikoroi, F.proliferatum (T. Matsushima) Nirenberg (sexual stage: G. proliferatum),and F. subglutinans (sexual stage: G. subglutinans). FSR infection ischaracterized by rotting roots, crown, and lower internodes that beginsshortly after pollination and progresses as the plant matures.Eventually the pith will disintegrate resulting in weak, spongy stalksthat are prone to lodging.

Due to the lack of fungicides and or other chemical controls for FSR,growers are faced with limited options for managing the disease. Sincethe most effective approach is to select hybrids that are intrinsicallyresistant, what is needed are methods of identifying genetic sources ofFSR resistance and more effective methods of introgressing those geneticelements into commercial lines to provide new hybrids with improvedgenetic resistance to FSR infection.

SUMMARY OF THE INVENTION

Identifying and selecting plants that exhibit resistance to Fusariumstalk rot (FSR) using marker-assisted selection (MAS) provides aneffective and efficient method of improving the survivability of corn toFSR infection. This invention provides marker loci and quantitativetrait loci (QTL) chromosome intervals that demonstrate significantco-segregation with FSR resistance. These markers, or additional locilinked to these markers, can be used in MAS breeding programs to produceplants with improved FSR resistance.

Marker loci and quantitative trait loci (QTL) chromosome intervals thatdemonstrate significant co-segregation with FSR resistance are provided.These markers, or additional loci linked to these markers, can be usedin MAS breeding programs to produce plants with improved FSR resistance.

The FSR-3.01 and FSR-8.01 loci correspond to QTL discovered onchromosome 3 and chromosome 8, respectively, of the corn genome. Theseloci contain genotypes closely linked to FSR resistance. Embodiments ofthis invention include methods of detecting genotypes within and/orlinked to FSR-3.01 or FSR-8.01 to create disease resistant corn lines.Provided herein are examples of markers that are useful for detectingthe presence or absence of disease resistance alleles linked to FSR-3.01or FSR-8.01 as part of a MAS breeding program to produce plants withimproved resistance to FSR infection.

Embodiments of this invention include identifying one or more cornplants with FSR resistance, improved resistance, or susceptibility toFSR infection by using a marker within the FSR-3.01 or FSR-8.01chromosome intervals, or a marker closely linked to FSR-3.01 orFSR-8.01. As used herein, “closely linked” means that the marker orlocus is within about 20 cM, preferably within about 15 cM, morepreferably within about 10 cM, even more preferably within about 5 cM,even more preferably within about 1 cM, even more preferably about 0.5cM, and even more preferably less than 0.5 cM of the identified FSRlocus.

The location in the maize genome of FSR-3.01 and FSR-8.01, andchromosome intervals and sub-intervals containing markers closely linkedto FSR-3.01 and FSR-8.01, are referenced herein to a public maize genomemap (IBM2 2008 Neighbors). Genomic markers such as psk2 and gpm753d canbe used to define the flanks of the FSR-3.01 chromosome interval, whichincludes markers closely linked to the FSR-3.01. Genomic markers such asumc1790 and mHbrBC384-Mo17 can be used to define the flanks of theFSR-8.01 chromosome interval, which includes markers closely linked toFSR-8.01. Other genomic markers may be used to define chromosomesub-intervals linked to FSR-3.01 or FSR-8.01.

Embodiments of this invention include methods of creating a populationof corn plants with enhanced FSR resistance by providing a firstpopulation of corn plants, detecting the presence of a genetic markerthat is genetically linked to FSR-3.01 by 20 cM or less in the firstpopulation, selecting one or more corn plants containing said markerfrom the first population of corn plants, and producing a population ofoffspring from at lease one of said selected corn plants. In otherembodiments, the genetic marker detected is genetically linked toFSR-3.01 by less than 15 cM, 10 cM, 5 cM, 1 cM, or 0.5 cM of FSR-3.01.

In another embodiment of this invention, a population of corn plantswith enhanced FSR resistance is created by providing a first populationof corn plants, detecting the presence of a genetic marker within theFSR3.01 chromosome interval, selecting one or more corn plantscontaining said marker from the first population of corn plants, andproducing a population of offspring from at lease one of said selectedcorn plants. The FSR-3.01 chromosome interval includes any markerflanked by psk2 and gpm753d, including psk2 and gpm753d. Sub-intervalsof the FSR-3.01 chromosome interval are also useful for this invention,and include any interval wherein one or both boarders of thesub-interval are between psk2 and gpm753d, including psk2 and gpm753d.In one embodiment, an FSR-3.01 sub-interval is flanked by, and includes,TIDP3078 and umc60. In another embodiment, an FSR-3.01 sub-interval isflanked by, and includes, SEQ ID NO: 1 and SEQ ID NO: 5. In anotherembodiment, an FSR-3.01 sub-interval is flanked by, and includes,TIDP6282 and SEQ ID NO: 4. In another embodiment, an FSR-3.01sub-interval is flanked by, and includes, SEQ ID NO: 90 and SEQ ID NO:2. All manner of chromosome interval lengths between, and including,psk2 and gpm753d can be used in conjunction with this invention.

In another embodiment of this invention, a population of corn plantswith enhanced FSR resistance is created by providing a first populationof corn plants, detecting the presence of a genetic marker that isselected from the group consisting of SEQ ID NOs: 1-5 and SEQ ID NOs:86-101 in the first population, selecting one or more corn plantscontaining said marker from the first population of corn plants, andproducing a population of offspring from at lease one of said selectedcorn plants.

Other embodiments of this invention include creating a population ofcorn plants with enhanced FSR resistance, wherein a portion of the FSRis caused by Fusarium moniliforme, by providing a first population ofcorn plants, detecting the presence of a genetic marker that isgenetically linked to FSR-3.01 by 20 cM or less in the first population,selecting one or more corn plants containing said marker from the firstpopulation of corn plants, and producing a population of offspring fromat lease one of said selected corn plants.

Embodiments of this invention also include methods of creating apopulation of corn plants with enhanced FSR resistance by providing afirst population of corn plants, detecting the presence of a geneticmarker that is genetically linked to FSR-8.01 by 20 cM or less in thefirst population, selecting one or more corn plants containing saidmarker from the first population of corn plants, and producing apopulation of offspring from at lease one of said selected corn plants.In other embodiments, the genetic marker detected is genetically linkedto FSR-8.01 by less than 15 cM, 10 cM, 5 cM, 1 cM, or 0.5 cM ofFSR-8.01.

In another embodiment of this invention, a population of corn plantswith enhanced FSR resistance is created by providing a first populationof corn plants, detecting the presence of a genetic marker within theFSR-3.01 chromosome interval, selecting one or more corn plantscontaining said marker from the first population of corn plants, andproducing a population of offspring from at lease one of said selectedcorn plants. The FSR-8.01 chromosome interval includes the FSR-8.01locus and any marker flanked by umc1790 and mHbrBC384-Mo17, includingumc1790 and mHbrBC384-Mo17. Sub-intervals of the FSR-8.01 chromosomeinterval are also useful, and include any interval wherein one or bothboarders of the sub-interval are between umc1790 and mHbrBC384-Mo17,including umc1790 or mHbrBC384-Mo17. In one embodiment, an FSR-3.01sub-interval is flanked by, and includes, TIDP3728 and TIDP5537. Inanother embodiment, an FSR-8.01 sub-interval is flanked by, andincludes, csu329 and IDP6942. All manner of chromosome interval lengthsbetween, and including, umc1790 and mHbrBC384-Mo17 can be used inconjunction with this invention.

In another embodiment of this invention, a population of corn plantswith enhanced FSR resistance is created by providing a first populationof corn plants, detecting the presence of a genetic marker that isselected from the group consisting of SEQ ID NOs: 6-17 in the firstpopulation, selecting one or more corn plants containing said markerfrom the first population of corn plants, and producing a population ofoffspring from at lease one of said selected corn plants.

Other embodiments of this invention include creating a population ofcorn plants with enhanced FSR resistance, wherein a portion of the FSRis caused by Fusarium moniliforme, by providing a first population ofcorn plants, detecting the presence of a genetic marker that isgenetically linked to FSR-3.01 by 20 cM or less in the first population,selecting one or more corn plants containing said marker from the firstpopulation of corn plants, and producing a population of offspring fromat lease one of said selected corn plants.

In one aspect, the present invention provides a method of obtaining acorn plant with enhanced Fusarium stalk rot resistance comprising: a)providing a population of corn plants; b) detecting in said plants thepresence of a Fusarium stalk rot resistance allele at a polymorphiclocus genetically linked to a chromosomal segment flanked by marker lociAY110352 and TIDP5096 or marker loci TIDP3099 and IDP4363; and c)selecting from said population at least a first plant comprising saidallele and enhanced Fusarium stalk rot resistance compared to a plantlacking said allele. In some embodiments, said segment is flanked bymarker loci TIDP3078 and umc60 or marker loci TIDP3728 and TIDP5537. Infurther embodiments, said segment is flanked by marker loci SEQ ID NO: 1and SEQ ID NO: 5 or marker loci csu329 and IDP6942. In yet furtherembodiments, said segment is flanked by marker loci SEQ ID NO: 90 andSEQ ID NO: 2 or marker loci SEQ ID NO: 6 and SEQ ID NO: 17. In otherembodiments, said polymorphic locus comprises a nucleic acid sequenceselected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ IDNO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ IDNO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ IDNO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17; SEQID NO: 86, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90,SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO:95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ IDNO: 100, and SEQ ID NO: 101. In some embodiments, said method furthercomprises selecting from said population at least two plants, therebyforming a population of corn plants comprising said allele and enhancedFusarium stalk rot resistance compared to a plant lacking said allele.In certain embodiments, said Fusarium stalk rot resistance allele wasintrogressed into said population of corn plants from a starting plantor population of corn plants containing said allele. In otherembodiments, said method further comprises producing a progeny plantwith Fusarium stalk rot resistance from said first plant. In someembodiments, producing the progeny plant comprises marker-assistedselection for Fusarium stalk rot resistance. In further embodiments,said progeny plant is an F2-F6 progeny plant. In yet furtherembodiments, producing said progeny plant comprises backcrossing.

In another aspect, the invention provides a method of producing a cornplant with enhanced Fusarium stalk rot resistance comprising: a)crossing a first corn plant comprising a Fusarium stalk rot resistanceallele with a second corn plant of a different genotype to produce oneor more progeny plants; and b) selecting a progeny plant based on thepresence of said allele at a polymorphic locus genetically linked to achromosomal segment flanked by marker loci AY110352 and TIDP5096 ormarker loci TIDP3099 and IDP4363; and wherein said allele confersenhanced resistance to Fusarium stalk rot compared to a plant lackingsaid allele. In some embodiments, said segment is flanked by marker lociTIDP3078 and umc60 or marker loci TIDP3728 and TIDP5537. In otherembodiments, said segment is flanked by marker loci SEQ ID NO: 1 and SEQID NO: 5 or marker loci csu329 and IDP6942. In yet other embodiments,said segment is flanked by marker loci SEQ ID NO: 90 and SEQ ID NO: 2 ormarker loci SEQ ID NO: 6 and SEQ ID NO: 17. In some embodiments, saidpolymorphic locus comprises a nucleic acid sequence selected from thegroup consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO:4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9,SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO:14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17; SEQ ID NO: 86, SEQ IDNO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO: 91, SEQID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96,SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, and SEQ IDNO: 101. In certain embodiments, said progeny plant is an F2-F6 progenyplant. In other embodiments, producing said progeny plant comprisesbackcrossing. In some embodiments, backcrossing comprises from 2-7generations of backcrosses. In further embodiments, backcrossingcomprises marker-assisted selection in at least two generations. In yetfurther embodiments, backcrossing comprises marker-assisted selection inall generations. In some embodiments, said first corn plant is an inbredor a hybrid. In other embodiments, said second corn plant is anagronomically elite corn plant. In further embodiments, saidagronomically elite corn plant is an inbred or a hybrid.

DETAILED DESCRIPTION OF THE INVENTION I. Chromosome Intervals

The term “chromosome interval” designates a contiguous linear span ofgenomic DNA that resides in planta on a single chromosome. The term alsodesignates any and all genomic intervals defined by any of the markersset forth in this invention. The genetic elements located on a singlechromosome interval are physically linked and the size of a chromosomeinterval is not particularly limited. In some aspects, the geneticelements located within a single chromosome interval are geneticallylinked, typically with a genetic recombination distance of, for example,less than or equal to 20 cM, or alternatively, less than or equal to 10cM. That is, two genetic elements within a single chromosome intervalundergo meiotic recombination at a frequency of less than or equal to20% or 10%, respectively.

The boundaries of a chromosome interval can be defined by geneticrecombination distance or by markers. In one embodiment, the boundariesof a chromosome interval comprise markers. In another embodiment, theboundaries of a chromosome interval comprise markers that will be linkedto the gene controlling the trait of interest, i.e., any marker thatlies within a given interval, including the terminal markers thatdefining the boundaries of the interval, and that can be used as amarker for the presents or absence of disease resistance. In oneembodiment, the intervals described herein encompass marker clustersthat co-segregate with disease resistance. The clustering of markersoccurs in relatively small domains on the chromosomes, indicating thepresence of a genetic locus controlling the trait of interest in thosechromosome regions. The interval encompasses markers that map within theinterval as well as the markers that define the terminal.

An interval described by the terminal markers that define the endpointsof the interval will include the terminal markers and any markerlocalizing within that chromosome domain, whether those markers arecurrently known or unknown. Although it is anticipated that one skilledin the art may describe additional polymorphic sites at marker loci inand around the markers identified herein, any marker within thechromosome intervals described herein that are associated with diseaseresistance fall within the scope of this claimed invention.

“Quantitative trait loci” or a “quantitative trait locus” (QTL) is agenetic domain that effects a phenotype that can be described inquantitative terms and can be assigned a “phenotypic value” whichcorresponds to a quantitative value for the phenotypic trait. A QTL canact through a single gene mechanism or by a polygenic mechanism. In someaspects, the invention provides QTL chromosome intervals, where a QTL(or multiple QTLs) that segregates with disease resistance is containedin those intervals. In one embodiment of this invention, the boundariesof chromosome intervals are drawn to encompass markers that will belinked to one or more QTL. In other words, the chromosome interval isdrawn such that any marker that lies within that interval (including theterminal markers that define the boundaries of the interval) isgenetically linked to the QTL. Each interval comprises at least one QTL,and furthermore, may indeed comprise more than one QTL. Close proximityof multiple QTL in the same interval may obfuscate the correlation of aparticular marker with a particular QTL, as one marker may demonstratelinkage to more than one QTL. Conversely, e.g., if two markers in closeproximity show co-segregation with the desired phenotypic trait, it issometimes unclear if each of those markers identifying the same QTL ortwo different QTL. Regardless, knowledge of how many QTL are in aparticular interval is not necessary to make or practice the invention.

FSR-3.01 and FSR-8.01 Chromosome Intervals

In one embodiment, the present invention provides a plant comprising anucleic acid molecule selected from the group consisting of SEQ ID NO:1-17 and 86-87, fragments thereof, and complements of both. In anotherembodiment, the present invention also provides a plant comprising thealleles of the FSR-3.01 or FSR-8.01 chromosome intervals, or fragmentsand complements thereof, as well as any plant comprising any combinationof one or more disease resistance loci linked to at least one markerselected from the group consisting of SEQ ID NOs: 1-17. Such alleles maybe homozygous or heterozygous.

The locations in the maize genome of FSR-3.01 and the chromosomeintervals comprising markers closely linked to it are disclosed in Table1a. The locations in the maize genome of FSR-8.01 and the chromosomeintervals comprising markers closely linked to it are disclosed in Table1b. Genetic map loci are represented in cM, with position zero being thefirst (most distal) marker known at the beginning of the chromosome onboth Monsanto's internal consensus genetic map and the Neighbors 2008maize genomic map, which is freely available to the public from theMaize GDB website and commonly used by those skilled in the art. Alsodisclosed in Table 1a are the physical locations of loci as they arereported on the B73 RefGen_v2 sequence public assembly by the ArizonaGenomics Institute, available on the internet.

TABLE 1a Genetic and physical map positions of markers and chromosomeintervals associated with FSR-3.01. Relative Genetic Map Position† MONIBM2 Physical Map Position†† Marker/Locus Map cM Map IcM Contig ChrStart Chr End AY110352 92.1 315.4 AC210054.3 137,393,153 137,393,815umc2600 92.7 318.6 * * * psk2 92.9 319.2 * * * TIDP3705 93.9 322.7AC211202.4 146,523,093 146,525,347 TIDP3078 97.3 336.8 AC207629.3151,704,461 151,708,725 umc1307 98.2 339.6 * * * SEQ ID NO. 86 100.8348.1 * * * IDP1974 102.1 349.8 AC197365.3 158,226,570 158,227,199 SEQID NO. 1 102.2 352.2 * * * SEQ ID NO. 87 102.49 * * * * cdo109 103.0352.0 * * * umc2265 103.9 354.0 * * * SEQ ID NO. 88 103.95 * * * *TIDP5176 104.0 354.1 AC209769.3 157,199,237 157,200,546 SEQ ID NO. 89104.2 358.1 * * * SEQ ID NO. 90 105 360.5 * * * SEQ ID NO. 91106.19 * * * * SEQ ID NO. 92 106.22 * * * * SEQ ID NO. 93 107.14 * * * *TIDP6282 107.8 367.6 * 160,569,420 160,571,111 mHbrBC105-Mo17 108.1368.4 * * * SEQ ID NO. 94 108.5 368.3 * * * SEQ ID NO. 95 108.7 * * * *SEQ ID NO. 96 108.8 369.4 * * * SEQ ID NO. 97 109.01 * * * * SEQ ID NO.98 109.3 371.4 * * * SEQ ID NO. 99 110.3 378.4 * * * TIDP6314 110.9381.8 AC197369.3 162,296,473 162,297,710 SEQ ID NO. 2 110.9 382.6 * * *umc1400 111.3 384.1 * * * AY111296 111.4 384.9 AC196009.2 166,450,397166,451,768 phm2885 111.7 386.4 * * * SEQ ID NO. 3 111.8 388.9 * * * SEQID NO. 100 111.8 388.9 * * * SEQ ID NO. 101 112.2 390.8 * * * bnl5.37b112.2 390.8 AC210716.4 168,366,251 168,368,471 ig1 112.6 392.0 * * *FSR-3.01 112.9 392.8 * * * IDP7722 113.2 393.6 AC209753.3 169,614,610169,617,490 IDP4102 114.0 396.0 170,056,595 170,062,921 IDP5975 117.3405.2 AC215304.3 172,136,757 172,138,424 pza02402 117.7 407.1 * * * SEQID NO. 4 118.0 408.4 * * * agrr184b 118.2 409.5 * * * IDP73 118.3 409.8AC207759.3 173,015,455 173,016,795 IDP854 122.8 426.2 AC218092.3177,251,217 177,253,603 SEQ ID NO. 5 122.8 426.3 * * * mHbrBG120-B73123.1 427.7 * * * IDP9062 123.4 429.4 AC191121.3 177,993,852 177,999,020TIDP3062 127.7 451.0 AC209080.3 179,515,709 179,517,154 umc1951 129.7452.7 * * * umc60 128.0 452.7 AC209784.3 181,079,164 181,079,889 gpm298132.8 464.9 * * * gpm753d 133.1 465.9 * * * TIDP5096 133.9 468.2SV208660.3 183,383,223 183,384,638 †cM = centiMorgans, IcM = map unitsof the IBM2 2008 Neighbors Genetic Map. ††Arizona Genomics Institute B73RefGen_v2 sequence. *Exact coordinates not known. Coordinates can beestimated based on nearest flanking loci with known coordinates.

TABLE 1b Genetic and physical map positions of markers and chromosomeintervals associated with FSR-8.01 Relative Genetic Map Position† MONIBM2 Physical Map Position†† Marker/Locus Map cM Map IcM Contig ChrStart Chr End TIDP3099 50.3 145.24 AC211474.3 16,743,915 16,746,003umc1790 50.6 146.24 * * * pco120121b 51.5 148.68 * * * umc1974 52.8153.3 AC187868.3 16,951,357 16,952,136 TIDP3728 55.6 161.57 AC211862.418,445,056 18,450,115 pza02454 56.2 163.2 * * * csu329 60.9 175.9AC187096.5 20,836,155 20,836,957 TIDP5282 61 176.22 AC194455.321,845,185 21,846,346 si605038f07 65.9 197.39 * * * mmp158b 66197.9 * * * umc1157 67.9 206 AC203336.3 70,970,790 70,971,548 umc180268.7 208.6 * * * SEQ ID NO: 6 69 233.6 * * * AY110113 69 209.5 * * *IDP4740 69.2 210.4 AC203025.3 37,639,446 37,641,719 mHbrMC218-Mo17 69.8211.95 * * * TIDP2981 70.1 212.6 AC233874.3 39,208,198 39,209,848umc1377 70.2 212.78 * * * FSR8.01 71 214.9 * * * TIDP3186 71.2 215.18AC200279.3 74,529,969 74,532,587 IDP8100 71.3 215.4 AC186573.372,875,502 72,876,977 umc2354 71.7 216.2 * * * SEQ ID NO: 7 71.9216.7 * * * bnl10.39 72 217.1 * * * phm11114 72.3 220.6 * * * SEQ ID NO:8 72.7 223 * * * cdo202e(mcf) 73 224.8 * * * umc1415 73.9 228.6AC198494.3 90,090,088 90,090,703 SEQ ID NO: 9 74.2 236 * * * SEQ ID NO:10 74.8 236.2 * * * umc2075 74.9 244.9 AC187095.4 95,022,313 95,023,319umc1615 75.1 246.2 * * * SEQ ID NO: 11 75.3 240.4 * * * gpm842 75.3247.8 * * * IDP7861 75.3 248 AC195139.3 91,198,710 91,204,796 TIDP278775.9 251.6 AC206644.4 93,196,687 93,197,665 SEQ ID NO: 12 77 254.7 * * *umc1302 77.1 260.4 * * * TIDP5619 77.8 265.2 AC194944.3 98,401,90798,403,980 SEQ ID NO: 13 78.3 265.6 * * * SEQ ID NO: 14 78.3 265.6 * * *AY109740 78.3 268.6 * * * IDP179 79.3 275.2 AC199187.3 100,957,496 100,958,829  pza03135 79.4 275.7 * * * SEQ ID NO: 15 79.5 275.7 * * *bnlg119 79.7 277.8 * * * umc1735 80 279.9 AC226575.4 101,349,090 101,349,799  IDP6942 81 283.1 AC204384.3 101,408,509  101,410,224  SEQID NO: 16 81.1 283.3 * * * umc1457 81.5 284.6 AC234152.3 102,138,514 102,138,808  SEQ ID NO: 17 81.7 285.3 * * * agrc478 82.7 287.4 * * *TIDP5537 83.6 289.5 AC234152.3 102,168,566  102,170,132  IDP294 90.9315.7 AC197600.3 117,896,373  117,896,994  mHbrBC384-Mo17 91.2316.69 * * * IDP4363 91.7 318.05 AC197705.4 118,075,051  118,077,149 †cM = centiMorgans, IcM = map units of the IBM2 2008 Neighbors GeneticMap. ††Arizona Genomics Institute B73 RefGen_v2 sequence. * Exactcoordinates not known. Coordinates can be estimated based on nearestflanking loci with known coordinates.

In Table 1, “IcM” refers to the map units of the IBM2 2008 NeighborsGenetic Map, which was generated with an intermated recombinant inbredpopulation (syn 4) that resulted in approximately a four-fold increasein the number of meiosies as compared to the typical recombinationexperiment that is used to generate centiMorgan (cM) distances (Lee etal., 2002, Plant Mol Biol 48:453 and the Maize Genetics and GenomicsDatabase). “cM” refers to the classical definition of a centimorgan(Haldane 1919 J Genet 8:299-309) wherein one cM is equal to a 1% chancethat a trait at one genetic locus will be separated from a trait atanother locus due to crossing over in a single meiosis (meaning thetraits cosegregate 99% of the time), and this definition is used hereinto delineate map locations pertaining to this invention.

For example, the FSR-3.01 chromosome interval contains SEQ ID NOs: 1-5and SEQ ID Nos. 86-101, and is flanked by the markers AY110352 andTIDP5096, which are separated by approximately 40 cM on theinternally-derived genetic map. This chromosome interval encompasses amarker cluster that co-segregates with FSR resistance in the populationsstudied at a p-value≦0.05. The FSR-3.01 interval provided hereincomprises markers AY110352, umc2600, psk2, TIDP3705, TIDP3078, umc1307,IDP1974, SEQ ID NO: 1, cdo109, umc2265, TIDP5176, TIDP6282,mHbrBC105-Mo17, TIDP6314, SEQ ID NO: 2, umc1400, AY111296, phm2885, SEQID NO: 3, bnl5.37b, ig1, FSR-3.01, IDP7722, IDP4102, IDP5975, pza02402,SEQ ID NO: 4, agrr184b, IDP73, IDP854, SEQ ID NO: 5, mHbrBG120-B73,IDP9062, TIDP3062, umc1951, umc60, gpm298, gpm753d, and TIDP5096. Anexample of a subinterval of the FSR-3.01 interval is that which isflanked by SEQ ID NO: 1 and SEQ ID NO: 5, separated by approximately20.6 cM on the internally-derived genetic map, that define a chromosomeinterval encompassing a cluster of markers that co-segregate with FSRresistance in the populations studied at a p-level≦0.05. In someembodiments, the marker co-segregating with FSR resistance is selectedfrom the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3,SEQ ID NO: 4, and SEQ ID NO: 5. A further example of a subinterval ofthe FSR-3.01 interval is that which is flanked by SEQ ID NO: 86 and SEQID NO: 101 encompassing a cluster of markers that co-segregate with FSRresistance. In some embodiments, the marker co-segregating with FSRresistance is selected from the group consisting of: SEQ ID NO: 86, SEQID NO: 1, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90,SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO:95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ IDNO: 2, SEQ ID NO: 100, and SEQ ID NO: 101.

Similarly, the FSR-8.01 chromosome interval contains SEQ ID NOs: 6-17and is flanked by the markers TIDP3099 and IDP4363, which are separatedby approximately 40 cM on the internally-derived genetic map. Thischromosome interval encompasses a marker cluster that co-segregates withFSR resistance in the populations studied at a p-value≦0.05. TheFSR-8.01 interval provided herein comprises markers TIDP3099, umc1790,pco120121b, umc1974, TIDP3728, pza02454, csu329, TIDP5282, si605038f07,mmp158b, umc1157, umc1802, SEQ ID NO: 6, AY110113, IDP4740,mHbrMC218-Mo17, TIDP2981, umc1377, FSR8.01, TIDP3186, IDP8100, umc2354,SEQ ID NO: 7, bnl10.39, phm11114, SEQ ID NO: 8, cdo202e(mcf), umc1415,SEQ ID NO: 9, SEQ ID NO: 10, umc2075, umc1615, SEQ ID NO: 11, gpm842,IDP7861, TIDP2787, SEQ ID NO: 12, umc1302, TIDP5619, SEQ ID NO: 13, SEQID NO: 14, AY109740, IDP179, pza03135, SEQ ID NO: 15, bnlg119, umc1735,IDP6942, SEQ ID NO: 16, umc1457, SEQ ID NO: 17, agrc478, TIDP5537,IDP294, mHbrBC384-Mo17, and IDP4363. An example of a subinterval of theFSR-8.01 interval is that which is flanked by SEQ ID NO: 6 and SEQ IDNO: 17, separated by approximately 12.7 cM on the internally-derivedgenetic map, that define a chromosome interval encompassing a cluster ofmarkers that co-segregate with FSR resistance in the populations studiedat a p-level≦0.05. In some embodiments, the marker co-segregating withFSR resistance is selected from the group consisting of: SEQ ID NO: 6,SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11,SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO:16, and SEQ ID NO: 17.

Thus, one skilled in the art can use this invention to improve theefficiency of breeding for improved disease resistance in maize byassociating disease resistance phenotypes with genotypes at previouslyunknown disease resistance loci in the maize genome. Disclosed hereinare chromosome intervals that comprise alleles responsible forphenotypic differences between disease resistant and disease susceptiblecorn lines. Each chromosome interval is characterized by the genomicregions including and flanked by and including the markers psk2 andgpm753d on chromosome 3 or umc1790 and mHbrBC384-Mo17 on chromosome 8,and comprise markers within or closely linked to (within 20 cM of)FSR-3.01 or FSR-8.01, respectively. This invention also comprises otherintervals whose boarders fall between, and including, those of psk2 andgpm753d or umc1790 and mHbrBC384-Mo17, or any interval closely linked tothose intervals.

Examples of markers useful for this purpose comprise the SNP markerslisted in Tables 1a or 1b, or any marker that maps within the chromosomeintervals described herein (including the termini of the intervals), orany marker linked to those markers. Such markers can be assayedsimultaneously or sequentially in a single sample or population ofsamples.

Accordingly, the markers and methods of the present invention can beutilized to guide MAS or breeding maize varieties with the desiredcomplement (set) of allelic forms of chromosome intervals associatedwith superior agronomic performance (resistance, along with any otheravailable markers for yield, disease resistance, etc.). Any of thedisclosed marker alleles can be introduced into a corn line viaintrogression, by traditional breeding (or introduced viatransformation, or both) to yield a corn plant with superior agronomicperformance. The number of alleles associated with resistance that canbe introduced or be present in a corn plant of the present inventionranges from one to the number of alleles disclosed herein, each integerof which is incorporated herein as if explicitly recited.

MAS using additional markers flanking either side of the DNA locusprovide further efficiency because an unlikely double recombinationevent would be needed to simultaneously break linkage between the locusand both markers. Moreover, using markers tightly flanking a locus, oneskilled in the art of MAS can reduce linkage drag by more accuratelyselecting individuals that have less of the potentially deleteriousdonor parent DNA. Any marker linked to or among the chromosome intervalsdescribed herein could be useful and within the scope of this invention.

Similarly, by identifying plants lacking the desired marker locus,susceptible or less resistant plants can be identified, and, e.g.,eliminated from subsequent crosses. Similarly, these marker loci can beintrogressed into any desired genomic background, germplasm, plant,line, variety, etc., as part of an overall MAS breeding program designedto enhance yield. The invention also provides chromosome QTL intervalsthat find equal use in MAS to select plants that demonstrate diseaseresistance or improved tolerance. Similarly, the QTL intervals can alsobe used to counter-select plants that are susceptible or have reducedresistance to disease.

The present invention also extends to a method of making a progeny cornplant and these progeny corn plants, per se. The method comprisescrossing a first parent corn plant with a second corn plant and growingthe female corn plant under plant growth conditions to yield corn plantprogeny. Methods of crossing and growing corn plants are well within theability of those of ordinary skill in the art. Such corn plant progenycan be assayed for alleles associated with resistance and, thereby, thedesired progeny selected. Such progeny plants or seed can be soldcommercially for corn production, used for food, processed to obtain adesired constituent of the corn, or further utilized in subsequentrounds of breeding. At least one of the first or second corn plants is acorn plant of the present invention in that it comprises at least one ofthe allelic forms of the markers of the present invention, such that theprogeny are capable of inheriting the allele.

Often, a method of the present invention is applied to at least onerelated corn plant such as from progenitor or descendant lines in thesubject corn plants' pedigree such that inheritance of the desiredresistance allele can be traced. The number of generations separatingthe corn plants being subject to the methods of the present inventionwill generally be from 1 to 20, commonly 1 to 5, and typically 1, 2, or3 generations of separation, and quite often a direct descendant orparent of the corn plant will be subject to the method (i.e., onegeneration of separation).

Thus, with this invention, one skilled in the art can detect thepresence or absence of disease resistance genotypes in the genomes ofcorn plants as part of a marker assisted selection program. In oneembodiment, a breeder ascertains the genotype at one or more markers fora disease resistant parent, which contains a disease resistance allele,and the genotype at one or more markers for a susceptible parent, whichlacks the resistance allele. For example, the markers of the presentinvention can be used in MAS in crosses involving elite×exotic cornlines by subjecting the segregating progeny to MAS to maintain diseaseresistance alleles, or alleles associated with yield under diseaseconditions. A breeder can then reliably track the inheritance of theresistance alleles through subsequent populations derived from crossesbetween the two parents by genotyping offspring with the markers used onthe parents and comparing the genotypes at those markers with those ofthe parents. Depending on how tightly linked the marker alleles are withthe trait, progeny that share genotypes with the disease resistantparent can be reliably predicted to express the resistant phenotype;progeny that share genotypes with the disease susceptible parent can bereliably predicted to express the susceptible phenotype. Thus, thelaborious and inefficient process of manually phenotyping the progenyfor disease resistance is avoided.

By providing the positions in the maize genome of the intervals and thedisease resistance associated markers within, this invention also allowsone skilled in the art to identify other markers within the intervalsdisclosed herein or linked to the chromosome intervals disclosed herein.

Closely linked markers flanking the locus of interest that have allelesin linkage disequilibrium with a resistance allele at that locus may beeffectively used to select for progeny plants with enhanced resistanceto disease conditions. Thus, the markers described herein, such as thoselisted in Tables 1a or 1b, as well as other markers genetically orphysically mapped to the same chromosome interval, may be used to selectfor maize plants with enhanced resistance to disease conditions.Typically, a set of these markers will be used, (e.g., 2 or more, 3 ormore, 4 or more, 5 or more) in the flanking region above the gene and asimilar set in the flanking region below the gene. Optionally, asdescribed above, a marker within the actual gene and/or locus may alsobe used. The parents and their progeny are screened for these sets ofmarkers, and the markers that are polymorphic between the two parentsare used for selection. In an introgression program, this allows forselection of the gene or locus genotype at the more proximal polymorphicmarkers and selection for the recurrent parent genotype at the moredistal polymorphic markers.

The choice of markers actually used to practice this invention is notparticularly limited and can be any marker that maps within the FSR-3.01or FSR-8.01 chromosome intervals described herein, any marker closelylinked (within 10 cM) to a marker in the FSR-3.01 or FSR-8.01 chromosomeintervals, or any marker selected from SEQ ID NO: 1-17 and 86-87, or themarkers listed in Tables 1a or 1b. Furthermore, since there are manydifferent types of marker detection assays known in the art, it is notintended that the type of marker detection assay (e.g. RAPDs, RFLPs,SNPs, AFLPs, etc.) used to practice this invention be limited in anyway.

II. Molecular Genetic Markers

“Marker,” “genetic marker,” “molecular marker,” “marker nucleic acid,”and “marker locus” refer to a nucleotide sequence or encoded productthereof (e.g., a protein) used as a point of reference when identifyinga linked locus. A marker can be derived from genomic nucleotide sequenceor from expressed nucleotide sequences (e.g., from a spliced RNA, acDNA, etc.), or from an encoded polypeptide, and can be represented byone or more particular variant sequences, or by a consensus sequence. Inanother sense, a marker is an isolated variant or consensus of such asequence. The term also refers to nucleic acid sequences complementaryto or flanking the marker sequences, such as nucleic acids used asprobes or primer pairs capable of amplifying the marker sequence. A“marker probe” is a nucleic acid sequence or molecule that can be usedto identify the presence of a marker locus, e.g., a nucleic acid probethat is complementary to a marker locus sequence. Alternatively, in someaspects, a marker probe refers to a probe of any type that is able todistinguish (i.e., genotype) the particular allele that is present at amarker locus. A “marker locus” is a locus that can be used to track thepresence of a second linked locus, e.g., a linked locus that encodes orcontributes to expression of a phenotypic trait. For example, a markerlocus can be used to monitor segregation of alleles at a locus, such asa QTL, that are genetically or physically linked to the marker locus.Thus, a “marker allele,” alternatively an “allele of a marker locus” isone of a plurality of polymorphic nucleotide sequences found at a markerlocus in a population that is polymorphic for the marker locus.

“Marker” also refers to nucleic acid sequences complementary to thegenomic sequences, such as nucleic acids used as probes. Markerscorresponding to genetic polymorphisms between members of a populationcan be detected by methods well-established in the art. These include,e.g., PCR-based sequence specific amplification methods, detection ofrestriction fragment length polymorphisms (RFLP), detection of isozymemarkers, detection of polynucleotide polymorphisms by allele specifichybridization (ASH), detection of amplified variable sequences of theplant genome, detection of self-sustained sequence replication,detection of simple sequence repeats (SSRs), detection of singlenucleotide polymorphisms (SNPs), or detection of amplified fragmentlength polymorphisms (AFLPs). Well established methods are also know forthe detection of expressed sequence tags (ESTs) and SSR markers derivedfrom EST sequences and randomly amplified polymorphic DNA (RAPD).

A favorable allele of a marker is the allele of the marker thatco-segregates with a desired phenotype (e.g., disease resistance). Asused herein, a QTL marker has a minimum of one favorable allele,although it is possible that the marker might have two or more favorablealleles found in the population. Any favorable allele of that marker canbe used advantageously for the identification and construction ofdisease resistant plant lines. Optionally, one, two, three or morefavorable allele(s) of different markers are identified in, orintrogressed into a plant, and can be selected for or against duringMAS. Desirably, plants or germplasm are identified that have at leastone such favorable allele that positively correlates with diseaseresistance or improved disease resistance. Alternatively, a markerallele that co-segregates with disease susceptibility also finds usewith the invention, since that allele can be used to identify andcounter select disease susceptible plants. Such an allele can be usedfor exclusionary purposes during breeding to identify alleles thatnegatively correlate with resistance, to eliminate susceptible plants orgermplasm from subsequent rounds of breeding.

The more tightly linked a marker is with a DNA locus influencing aphenotype, the more reliable the marker is in MAS, as the likelihood ofa recombination event unlinking the marker and the locus decreases.Markers containing the causal mutation for a trait, or that are withinthe coding sequence of a causative gene, are ideal as no recombinationis expected between them and the sequence of DNA responsible for thephenotype.

Genetic markers are distinguishable from each other (as well as from theplurality of alleles of anyone particular marker) on the basis ofpolynucleotide length and/or sequence. A large number of corn molecularmarkers are known in the art, and are published or available fromvarious sources, such as the MaizeGDB internet resource. In general, anydifferentially inherited polymorphic trait (including a nucleic acidpolymorphism) that segregates among progeny is a potential geneticmarker.

In some embodiments of the invention, one or more marker alleles areselected for in a single plant or a population of plants. In thesemethods, plants are selected that contain favorable alleles from morethan one resistance marker, or alternatively, favorable alleles frommore than one resistance marker are introgressed into a desiredgermplasm. One of skill recognizes that the identification of favorablemarker alleles is germplasm-specific. The determination of which markeralleles correlate with resistance (or susceptibility) is determined forthe particular germplasm under study. One of skill recognizes thatmethods for identifying the favorable alleles are known in the art.Identification and use of such favorable alleles is within the scope ofthis invention. Furthermore still, identification of favorable markeralleles in plant populations other than the populations used ordescribed herein is within the scope of this invention.

Marker Detection

In some aspects, methods of the invention utilize an amplification stepto detect/genotype a marker locus, but amplification is not always arequirement for marker detection (e.g. Southern blotting and RFLPdetection). Separate detection probes can also be omitted inamplification/detection methods, e.g., by performing a real timeamplification reaction that detects product formation by modification ofthe relevant amplification primer upon incorporation into a product,incorporation of labeled nucleotides into an amplicon, or by monitoringchanges in molecular rotation properties of amplicons as compared tounamplified precursors (e.g., by fluorescence polarization).

“Amplifying,” in the context of nucleic acid amplification, is anyprocess whereby additional copies of a selected nucleic acid (or atranscribed form thereof) are produced. In some embodiments, anamplification based marker technology is used wherein a primer oramplification primer pair is admixed with genomic nucleic acid isolatedfrom the first plant or germplasm, and wherein the primer or primer pairis complementary or partially complementary to at least a portion of themarker locus, and is capable of initiating DNA polymerization by a DNApolymerase using the plant genomic nucleic acid as a template. Theprimer or primer pair is extended in a DNA polymerization reactionhaving a DNA polymerase and a template genomic nucleic acid to generateat least one amplicon. In other embodiments, plant RNA is the templatefor the amplification reaction. In some embodiments, the QTL marker is aSNP type marker, and the detected allele is a SNP allele, and the methodof detection is allele specific hybridization (ASH).

In general, the majority of genetic markers rely on one or more propertyof nucleic acids for their detection. Typical amplification methodsinclude various polymerase based replication methods, including thepolymerase chain reaction (PCR), ligase mediated methods such as theligase chain reaction (LCR) and RNA polymerase based amplification(e.g., by transcription) methods. An “amplicon” is an amplified nucleicacid, e.g., a nucleic acid that is produced by amplifying a templatenucleic acid by any available amplification method (e.g., PCR, LCR,transcription, or the like). A “genomic nucleic acid” is a nucleic acidthat corresponds in sequence to a heritable nucleic acid in a cell.Common examples include nuclear genomic DNA and amplicons thereof. Agenomic nucleic acid is, in some cases, different from a spliced RNA, ora corresponding cDNA, in that the spliced RNA or cDNA is processed,e.g., by the splicing machinery, to remove introns. Genomic nucleicacids optionally comprise non-transcribed (e.g., chromosome structuralsequences, promoter regions, enhancer regions, etc.) and/ornon-translated sequences (e.g., introns), whereas spliced RNA/cDNAtypically do not have non-transcribed sequences or introns. A “templatenucleic acid” is a nucleic acid that serves as a template in anamplification reaction (e.g., a polymerase based amplification reactionsuch as PCR, a ligase mediated amplification reaction such as LCR, atranscription reaction, or the like). A template nucleic acid can begenomic in origin, or alternatively, can be derived from expressedsequences, e.g., a cDNA or an EST. Details regarding the use of theseand other amplification methods can be found in any of a variety ofstandard texts. Many available biology texts also have extendeddiscussions regarding PCR and related amplification methods and one ofskill will appreciate that essentially any RNA can be converted into adouble stranded DNA suitable for restriction digestion, PCR expansionand sequencing using reverse transcriptase and a polymerase.

PCR detection and quantification using dual-labeled fluorogenicoligonucleotide probes, commonly referred to as “TaqMan™” probes, canalso be performed according to the present invention. These probes arecomposed of short (e.g., 20-25 base) oligodeoxynucleotides that arelabeled with two different fluorescent dyes. On the 5′ terminus of eachprobe is a reporter dye, and on the 3′ terminus of each probe aquenching dye is found. The oligonucleotide probe sequence iscomplementary to an internal target sequence present in a PCR amplicon.When the probe is intact, energy transfer occurs between the twofluorophores and emission from the reporter is quenched by the quencherby FRET. During the extension phase of PCR, the probe is cleaved by 5′nuclease activity of the polymerase used in the reaction, therebyreleasing the reporter from the oligonucleotide-quencher and producingan increase in reporter emission intensity. TaqMan™ probes areoligonucleotides that have a label and a quencher, where the label isreleased during amplification by the exonuclease action of thepolymerase used in amplification, providing a real time measure ofamplification during synthesis. A variety of TaqMan™ reagents arecommercially available, e.g., from Applied Biosystems as well as from avariety of specialty vendors such as Biosearch Technologies.

In one embodiment, the presence or absence of a molecular marker isdetermined simply through nucleotide sequencing of the polymorphicmarker region. This method is readily adapted to high throughputanalysis as are the other methods noted above, e.g., using availablehigh throughput sequencing methods such as sequencing by hybridization.

In alternative embodiments, in silico methods can be used to detect themarker loci of interest. For example, the sequence of a nucleic acidcomprising the marker locus of interest can be stored in a computer. Thedesired marker locus sequence or its homolog can be identified using anappropriate nucleic acid search algorithm as provided by, for example,in such readily available programs as BLAST, or even simple wordprocessors.

While the exemplary markers provided in the figures and tables hereinare either SNP markers, any of the aforementioned marker types can beemployed in the context of the invention to identify chromosomeintervals encompassing genetic element that contribute to superioragronomic performance (e.g., disease resistance or improved diseasetolerance).

Probes and Primers

In general, synthetic methods for making oligonucleotides, includingprobes, primers, molecular beacons, PNAs, LNAs (locked nucleic acids),etc., are known. For example, oligonucleotides can be synthesizedchemically according to the solid phase phosphoramidite triester methoddescribed. Oligonucleotides, including modified oligonucleotides, canalso be ordered from a variety of commercial sources.

Nucleic acid probes to the marker loci can be cloned and/or synthesized.Any suitable label can be used with a probe of the invention. Detectablelabels suitable for use with nucleic acid probes include, for example,any composition detectable by spectroscopic, radioisotopic,photochemical, biochemical, immunochemical, electrical, optical orchemical means. Useful labels include biotin for staining with labeledstreptavidin conjugate, magnetic beads, fluorescent dyes, radio labels,enzymes, and colorimetric labels. Other labels include ligands whichbind to antibodies labeled with fluorophores, chemiluminescent agents,and enzymes. A probe can also constitute radio labeled PCR primers thatare used to generate a radio labeled amplicon.

It is not intended that the nucleic acid probes of the invention belimited to any particular size.

In some preferred embodiments, the molecular markers of the inventionare detected using a suitable PCR-based detection method, where the sizeor sequence of the PCR amplicon is indicative of the absence or presenceof the marker (e.g., a particular marker allele). In these types ofmethods, PCR primers are hybridized to the conserved regions flankingthe polymorphic marker region. As used in the art, PCR primers used toamplify a molecular marker are sometimes termed “PCR markers” or simply“markers.” It will be appreciated that, although many specific examplesof primers are provided herein, suitable primers to be used with theinvention can be designed using any suitable method. It is not intendedthat the invention be limited to any particular primer or primer pair.In some embodiments, the primers of the invention are radiolabelled, orlabeled by any suitable means (e.g., using a non-radioactive fluorescenttag), to allow for rapid visualization of the different size ampliconsfollowing an amplification reaction without any additional labeling stepor visualization step. In some embodiments, the primers are not labeled,and the amplicons are visualized following their size resolution, e.g.,following agarose gel electrophoresis. In some embodiments, ethidiumbromide staining of the PCR amplicons following size resolution allowsvisualization of the different size amplicons. It is not intended thatthe primers of the invention be limited to generating an amplicon of anyparticular size. For example, the primers used to amplify the markerloci and alleles herein are not limited to amplifying the entire regionof the relevant locus. The primers can generate an amplicon of anysuitable length that is longer or shorter than those disclosed herein.In some embodiments, marker amplification produces an amplicon at least20 nucleotides in length, or alternatively, at least 50 nucleotides inlength, or alternatively, at least 100 nucleotides in length, oralternatively, at least 200 nucleotides in length. Marker alleles inaddition to those recited herein also find use with the presentinvention.

III. Linkage Analysis and QTL Linkage Analysis

“Linkage”, or “genetic linkage,” is used to describe the degree withwhich one marker locus is “associated with” another marker locus or someother locus (for example, a resistance locus). For example, if locus Ahas genes “A” or “a” and locus B has genes “B” or “b” and a crossbetween parent 1 with AABB and parent 2 with aabb will produce fourpossible gametes where the genes are segregated into AB, Ab, aB and ab.The null expectation is that there will be independent equal segregationinto each of the four possible genotypes, i.e. with no linkage ¼ of thegametes will of each genotype. Segregation of gametes into a genotypesdiffering from ¼ is attributed to linkage. As used herein, linkage canbe between two markers, or alternatively between a marker and aphenotype. A marker locus can be associated with (linked to) a trait,e.g., a marker locus can be associated with resistance or improvedtolerance to a plant pathogen when the marker locus is in linkagedisequilibrium (LD) with the resistance trait. The degree of linkage ofa molecular marker to a phenotypic trait (e.g., a QTL) is measured,e.g., as a statistical probability of co-segregation of that molecularmarker with the phenotype.

As used herein, the linkage relationship between a molecular marker anda phenotype is given is the statistical likelihood that the particularcombination of a phenotype and the presence or absence of a particularmarker allele is random. Thus, the lower the probability score, thegreater the likelihood that a phenotype and a particular marker willcosegregate. In some embodiments, a probability score of 0.05 (p=0.05,or a 5% probability) of random assortment is considered a significantindication of co-segregation. However, the present invention is notlimited to this particular standard, and an acceptable probability canbe any probability of less than 50% (p<0.5). For example, a significantprobability can be less than 0.25, less than 0.20, less than 0.15, orless than 0.1. The phrase “closely linked,” in the present application,means that recombination between two linked loci occurs with a frequencyof equal to or less than about 10% (i.e., are separated on a genetic mapby not more than 10 cM). In one aspect, any marker of the invention islinked (genetically and physically) to any other marker that is at orless than 50 cM distant. In another aspect, any marker of the inventionis closely linked (genetically and physically) to any other marker thatis in close proximity, e.g., at or less than 10 cM distant. Two closelylinked markers on the same chromosome can be positioned 20, 19, 18, 17,16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.75, 0.5 or 0.25cM or less from each other.

Classical linkage analysis can be thought of as a statisticaldescription of the relative frequencies of co segregation of differenttraits. Linkage analysis is the well characterized descriptive frameworkof how traits are grouped together based upon the frequency with whichthey segregate together. That is, if two non-allelic traits areinherited together with a greater than random frequency, they are saidto be “linked.” The frequency with which the traits are inheritedtogether is the primary measure of how tightly the traits are linked,i.e., traits which are inherited together with a higher frequency aremore closely linked than traits which are inherited together with lower(but still above random) frequency. The further apart on a chromosomethe genes reside, the less likely they are to segregate together,because homologous chromosomes recombine during meiosis. Thus, thefurther apart on a chromosome the genes reside, the more likely it isthat there will be a crossing over event during meiosis that will resultin the marker and the DNA sequence responsible for the trait the markeris designed to track segregating separately into progeny. A commonmeasure of linkage is the frequency with which traits cosegregate. Thiscan be expressed as a percentage of cosegregation (recombinationfrequency) or, also commonly, in centiMorgans (cM).

Linkage analysis is used to determine which polymorphic marker alleledemonstrates a statistical likelihood of co-segregation with theresistance phenotype (thus, a “resistance marker allele”). Followingidentification of a marker allele for co-segregation with the resistancephenotype, it is possible to use this marker for rapid, accuratescreening of plant lines for the resistance allele without the need togrow the plants through their life cycle and await phenotypicevaluations, and furthermore, permits genetic selection for theparticular resistance allele even when the molecular identity of theactual resistance QTL is unknown. Tissue samples can be taken, forexample, from the endosperm, embryo, or mature/developing plant andscreened with the appropriate molecular marker to rapidly determinedetermined which progeny contain the desired genetics. Linked markersalso remove the impact of environmental factors that can often influencephenotypic expression.

Because chromosomal distance is approximately proportional to thefrequency of crossing over events between traits, there is anapproximate physical distance that correlates with recombinationfrequency. Marker loci are themselves traits and can be assessedaccording to standard linkage analysis by tracking the marker lociduring segregation. Thus, in the context of the present invention, onecM is equal to a 1% chance that a marker locus will be separated fromanother locus (which can be any other trait, e.g., another marker locus,or another trait locus that encodes a QTL), due to crossing over in asingle generation.

When referring to the relationship between two genetic elements, such asa genetic element contributing to resistance and a proximal marker,“coupling” phase linkage indicates the state where the “favorable”allele at the resistance locus is physically associated on the samechromosome strand as the “favorable” allele of the respective linkedmarker locus. In coupling phase, both favorable alleles are inheritedtogether by progeny that inherit that chromosome strand. In “repulsion”phase linkage, the “favorable” allele at the locus of interest (e.g., aQTL for resistance) is physically linked with an “unfavorable” allele atthe proximal marker locus, and the two “favorable” alleles are notinherited together (i.e., the two loci are “out of phase” with eachother).

Quantitative Trait Loci

An allele of a QTL can comprise multiple genes or other genetic factorseven within a contiguous genomic region or linkage group, such as ahaplotype. As used herein, an allele of a disease resistance locus canencompass more than one gene or nucleotide sequence where eachindividual gene or nucleotide sequence is also capable of exhibitingallelic variation and where each gene or nucleotide sequence is alsocapable of eliciting a phenotypic effect on the quantitative trait inquestion. In an aspect of the present invention the allele of a QTLcomprises one or more genes or nucleic acid sequences that are alsocapable of exhibiting allelic variation. The use of the term “an alleleof a QTL” is thus not intended to exclude a QTL that comprises more thanone gene or other genetic factor. Specifically, an “allele of a QTL” inthe present in the invention can denote a haplotype within a haplotypewindow wherein a phenotype can be disease resistance. A haplotype windowis a contiguous genomic region that can be defined, and tracked, with aset of one or more polymorphic markers wherein the polymorphismsindicate identity by descent. A haplotype within that window can bedefined by the unique fingerprint of alleles at each marker. When allthe alleles present at a given locus on a chromosome are the same, thatplant is homozygous at that locus. If the alleles present at a givenlocus on a chromosome differ, that plant is heterozygous at that locus.Plants of the present invention may be homozygous or heterozygous at anyparticular disease locus or for a particular polymorphic marker.

The principles of QTL analysis and statistical methods for calculatinglinkage between markers and useful QTL, or between any loci in a genomeare well known in the art. Exemplary methods include penalizedregression analysis, ridge regression, single point marker analysis,complex pedigree analysis, Bayesian MCMC, identity-by-descent analysis,interval mapping, composite interval mapping, and Haseman-Elstonregression. QTL analyses are often performed with the help of a computerand specialized software available from a variety of public andcommercial sources known to those of skill in the art.

IV. Genetic Mapping

A “genetic map” is the relationship of genetic linkage among loci on oneor more chromosomes (or linkage groups) within a given species,generally depicted in a diagrammatic or tabular form. “Genetic mapping”is the process of defining the linkage relationships of loci through theuse of genetic markers, populations segregating for the markers, andstandard genetic principles of recombination frequency. A “genetic maplocation” is a location on a genetic map relative to surrounding geneticmarkers on the same linkage group where a specified marker can be foundwithin a given species. In contrast, a physical map of the genome refersto absolute distances (for example, measured in base pairs or isolatedand overlapping contiguous genetic fragments, e.g., contigs). A physicalmap of the genome does not take into account the genetic behavior (e.g.,recombination frequencies) between different points on the physical map.A “genetic recombination frequency” is the frequency of a crossing overevent (recombination) between two genetic loci. Recombination frequencycan be observed by following the segregation of markers and/or traitsfollowing meiosis. A genetic recombination frequency can be expressed incentimorgans (cM). In some cases, two different markers can have thesame genetic map coordinates. In that case, the two markers are in suchclose proximity to each other that recombination occurs between themwith such low frequency that it is undetected.

Genetic maps are graphical representations of genomes (or a portion of agenome such as a single chromosome) where the distances between markersare measured by the recombination frequencies between them. Plantbreeders use genetic maps of molecular markers to increase breedingefficiency through Marker assisted selection (MAS), a process whereselection for a trait of interest is not based on the trait itself butrather on the genotype of a marker linked to the trait. A molecularmarker that demonstrates reliable linkage with a phenotypic traitprovides a useful tool for indirectly selecting the trait in a plantpopulation, especially when accurate phenotyping is difficult, slow, orexpensive.

In general, the closer two markers or genomic loci are on the geneticmap, the closer they lie to one another on the physical map. A lack ofprecise proportionality between cM distances and physical distances canexist due to the fact that the likelihood of genetic recombination isnot uniform throughout the genome; some chromosome regions arecross-over “hot spots,” while other regions demonstrate only rarerecombination events, if any.

Genetic mapping variability can also be observed between differentpopulations of the same crop species. In spite of this variability inthe genetic map that may occur between populations, genetic map andmarker information derived from one population generally remains usefulacross multiple populations in identification of plants with desiredtraits, counter-selection of plants with undesirable traits and inguiding MAS.

As one of skill in the art will recognize, recombination frequencies(and as a result, genetic map positions) in any particular populationare not static. The genetic distances separating two markers (or amarker and a QTL) can vary depending on how the map positions aredetermined. For example, variables such as the parental mappingpopulations used, the software used in the marker mapping or QTLmapping, and the parameters input by the user of the mapping softwarecan contribute to the QTL marker genetic map relationships. However, itis not intended that the invention be limited to any particular mappingpopulations, use of any particular software, or any particular set ofsoftware parameters to determine linkage of a particular marker orchromosome interval with the disease resistance phenotype. It is wellwithin the ability of one of ordinary skill in the art to extrapolatethe novel features described herein to any gene pool or population ofinterest, and using any particular software and software parameters.Indeed, observations regarding genetic markers and chromosome intervalsin populations in addition to those described herein are readily madeusing the teaching of the present disclosure.

Association Mapping

Association or LD mapping techniques aim to identify genotype-phenotypeassociations that are significant. It is effective for fine mapping inoutcrossing species where frequent recombination among heterozygotes canresult in rapid LD decay. LD is non-random association of alleles in acollection of individuals, reflecting the recombinational history ofthat region. Thus, LD decay averages can help determine the number ofmarkers necessary for a genome-wide association study to generate agenetic map with a desired level of resolution.

Large populations are better for detecting recombination, while olderpopulations are generally associated with higher levels of polymorphism,both of which contribute to accelerated LD decay. However, smallereffective population sizes tend to show slower LD decay, which canresult in more extensive haplotype conservation. Understanding of therelationships between polymorphism and recombination is useful indeveloping strategies for efficiently extracting information from theseresources. Association analyses compare the plants' phenotypic scorewith the genotypes at the various loci. Subsequently, any suitable maizegenetic map (for example, a composite map) can be used to help observedistribution of the identified QTL markers and/or QTL marker clusteringusing previously determined map locations of the markers.

V. Marker Assisted Selection, Plant Breeding, and Genomic IntrogressionMarker Assisted Selection

“Introgression” refers to the transmission of a desired allele of agenetic locus from one genetic background to another by. For example,introgression of a desired allele at a specified locus can betransmitted to at least one progeny via a sexual cross between twoparents of the same species, where at least one of the parents has thedesired allele in its genome. Alternatively, for example, transmissionof an allele can occur by recombination between two donor genomes, e.g.,in a fused protoplast, where at least one of the donor protoplasts hasthe desired allele in its genome. The desired allele can be, e.g., aselected allele of a marker, a QTL, a transgene, or the like. In anycase, offspring comprising the desired allele can be repeatedlybackcrossed to a line having a desired genetic background and selectedfor the desired allele, to result in the allele becoming fixed in aselected genetic background.

A primary motivation for development of molecular markers in cropspecies is the potential for increased efficiency in plant breedingthrough marker assisted selection (MAS). Genetic markers are used toidentify plants that contain a desired genotype at one or more loci, andthat are expected to transfer the desired genotype, along with a desiredphenotype to their progeny. Genetic markers can be used to identifyplants containing a desired genotype at one locus, or at severalunlinked or linked loci (e.g., a haplotype), and that would be expectedto transfer the desired genotype, along with a desired phenotype totheir progeny. The present invention provides the means to identifyplants that are resistant, exhibit improved resistance or aresusceptible to FSR infection by identifying plants having a specifiedallele that is linked to FSR-3.01 or FSR-8.01.

In general, MAS uses polymorphic markers that have been identified ashaving a significant likelihood of co-segregation with a resistancetrait. Such markers are presumed to map near a gene or genes that givethe plant its resistance phenotype, and are considered indicators forthe desired trait, and are termed QTL markers. Plants are tested for thepresence or absence of a desired allele in the QTL marker.

Identification of plants or germplasm that include a marker locus ormarker loci linked to a resistance trait or traits provides a basis forperforming marker assisted selection. Plants that comprise favorablemarkers or favorable alleles are selected for, while plants thatcomprise markers or alleles that are negatively correlated withresistance can be selected against. Desired markers and/or alleles canbe introgressed into plants having a desired (e.g., elite or exotic)genetic background to produce an introgressed resistant plant orgermplasm. In some aspects, it is contemplated that a plurality ofresistance markers are sequentially or simultaneous selected and/orintrogressed. The combinations of resistance markers that are selectedfor in a single plant is not limited, and can include any combination ofmarkers disclosed herein or any marker linked to the markers disclosedherein, or any markers located within the QTL intervals defined herein.

In some embodiments, the allele that is detected is a favorable allelethat positively correlates with disease resistance or improved diseasetolerance. In the case where more than one marker is selected, an alleleis selected for each of the markers; thus, two or more alleles areselected. In some embodiments, it can be the case that a marker locuswill have more than one advantageous allele, and in that case, eitherallele can be selected. It will be appreciated that the ability toidentify QTL marker loci alleles that correlate with resistance,improved tolerance, or susceptibility of a corn plant to diseaseconditions provides a method for selecting plants that have favorablemarker loci as well. That is, any plant that is identified as comprisinga desired marker locus (e.g., a marker allele that positively correlateswith resistance) can be selected for, while plants that lack the locus,or that have a locus that negatively correlates with resistance, can beselected against.

In some embodiments, a disease resistant first corn plant or germplasm(the donor) can be crossed with a second corn plant or germplasm (therecipient, e.g., an elite or exotic corn, depending on characteristicsthat are desired in the progeny) to create an introgressed corn plant orgermplasm as part of a breeding program designed to improve diseaseresistance of the recipient corn plant or germplasm. In some aspects,the recipient plant can also contain one or more disease resistant loci,which can be qualitative or quantitative trait loci. In another aspect,the recipient plant can contain a transgene.

In some embodiments, the recipient corn plant or germplasm willtypically display reduced resistance to disease conditions as comparedto the first corn plant or germplasm, while the introgressed corn plantor germplasm will display an increased resistance to disease conditionsas compared to the second plant or germplasm. An introgressed corn plantor germplasm produced by these methods are also a feature of thisinvention.

MAS is a powerful shortcut to selecting for desired phenotypes and forintrogressing desired traits into cultivars (e.g., introgressing desiredtraits into elite lines). MAS is easily adapted to high throughputmolecular analysis methods that can quickly screen large numbers ofplant or germplasm genetic material for the markers of interest and ismuch more cost effective than raising and observing plants for visibletraits.

When a population is segregating for multiple loci affecting one ormultiple traits, e.g., multiple loci involved in resistance, or multipleloci each involved in resistance or tolerance to different diseases, theefficiency of MAS compared to phenotypic screening becomes even greater,because all of the loci can be evaluated in the lab together from asingle sample of DNA.

Marker Assisted Backcrossing

One application of MAS is to use the resistance or improved tolerancemarkers to increase the efficiency of an introgression effort aimed atintroducing a resistance QTL into a desired (typically high yielding)background. If the nucleic acids from a plant are positive for a desiredgenetic marker allele, the plant can be self fertilized to create a truebreeding line with the same genotype, or it can be crossed with a plantwith the same marker or with other characteristics to create a sexuallycrossed hybrid generation.

Another use of MAS in plant breeding is to assist the recovery of therecurrent parent genotype by backcross breeding. Backcross breeding isthe process of crossing a progeny back to one of its parents or parentlines. Backcrossing is usually done for the purpose of introgressing oneor a few loci from a donor parent (e.g., a parent comprising desirableresistance marker loci) into an otherwise desirable genetic backgroundfrom the recurrent parent (e.g., an otherwise high yielding line). Themore cycles of back crossing that are done, the greater the geneticcontribution of the recurrent parent to the resulting introgressedvariety. This is often necessary, because resistant plants may beotherwise undesirable, e.g., due to low yield, low fecundity, or thelike. In contrast, strains which are the result of intensive breedingprograms may have excellent yield, fecundity or the like, merely beingdeficient in one desired trait such as resistance to FSR infection.

Moreover, in another aspect, while maintaining the introduced markersassociated with resistance, the genetic contribution of the plantproviding disease resistance can be reduced by back-crossing or othersuitable approaches. In one aspect, the nuclear genetic material derivedfrom the donor material in the plant can be less than or about 50%, lessthan or about 25%, less than or about 13%, less than or about 5%, 3%, 2%or 1%, but that the recipient remains resistant to disease.

Genetic diversity is important for long term genetic gain in anybreeding program. With limited diversity, genetic gain will eventuallyplateau when all of the favorable alleles have been fixed within theelite population. One objective is to incorporate diversity into anelite pool without losing the genetic gain that has already been madeand with the minimum possible investment. MAS provide an indication ofwhich genomic regions and which favorable alleles from the originalancestors have been selected for and conserved over time, facilitatingefforts to incorporate favorable variation from exotic germplasm sources(parents that are unrelated to the elite gene pool) in the hopes offinding favorable alleles that do not currently exist in the elite genepool.

VI. Transgenic Plants Transformation Constructs

Vectors used for plant transformation may include, for example,plasmids, cosmids, YACs (yeast artificial chromosomes), BACs (bacterialartificial chromosomes) or any other suitable cloning system, as well asfragments of DNA therefrom. Thus when the term “vector” or “expressionvector” is used, all of the foregoing types of vectors, as well asnucleic acid sequences isolated therefrom, are included. It iscontemplated that utilization of cloning systems with large insertcapacities will allow introduction of large DNA sequences comprisingmore than one selected gene. In accordance with the present disclosure,this could be used to introduce genes corresponding to, e.g., an entirebiosynthetic pathway, into a plant.

Particularly useful for transformation are expression cassettes whichhave been isolated from such vectors. DNA segments used for transformingplant cells will generally comprise the cDNA, gene, or genes which onedesires to introduce into and have expressed in the host cells. TheseDNA segments can further include structures such as promoters,enhancers, polylinkers, or regulatory genes as desired. The DNA segmentor gene chosen for cellular introduction will often encode a proteinwhich will be expressed in the resultant recombinant cells resulting ina screenable or selectable trait and/or which will impart an improvedphenotype to the resulting transgenic plant.

Regulatory elements such as promoters, leaders, enhancers, introns, andtranscription termination regions (or 3′ UTRs) can play an integral partin the overall expression of genes in living cells. The term “regulatoryelement,” as used herein, refers to a DNA molecule havinggene-regulatory activity. The term “gene-regulatory activity,” as usedherein, refers to the ability to affect the expression of an operablylinked transcribable DNA molecule, for instance by affecting thetranscription and/or translation of the operably linked transcribableDNA molecule. Regulatory elements, such as promoters, leaders,enhancers, and introns that function in plants are therefore useful formodifying plant phenotypes through genetic engineering.

As used herein, the term “intron” refers to a DNA molecule that may beisolated or identified from the genomic copy of a gene and may bedefined generally as a region spliced out during messenger RNA (mRNA)processing prior to translation. Alternately, an intron may be asynthetically produced or manipulated DNA element. An intron may containenhancer elements that effect the transcription of operably linkedgenes. An intron may be used as a regulatory element for modulatingexpression of an operably linked transcribable DNA molecule. A constructmay comprise an intron, and the intron may or may not be heterologouswith respect to the transcribable DNA molecule. Examples of introns inthe art include the rice actin intron and the corn HSP70 intron. Inplants, the inclusion of some introns in constructs leads to increasedmRNA and protein accumulation relative to constructs lacking the intron.This effect has been termed “intron mediated enhancement” (IME) of geneexpression. Introns known to stimulate expression in plants have beenidentified in maize genes (e.g., tubA1, Adh1, Sh1, and Ubi1), in ricegenes (e.g., tpi) and in dicotyledonous plant genes like those frompetunia (e.g., rbcS), potato (e.g., st-ls1) and from Arabidopsisthaliana (e.g., ubq3 and pat1). It has been shown that deletions ormutations within the splice sites of an intron reduce gene expression,indicating that splicing might be needed for IME. However, that splicingper se is not required, as IME in dicotyledonous plants has been shownby point mutations within the splice sites of the pat1 gene from A.thaliana. Multiple uses of the same intron in one plant have been shownto exhibit disadvantages. In those cases, it is necessary to have acollection of basic control elements for the construction of appropriaterecombinant DNA elements.

As used herein, the term “enhancer” or “enhancer element” refers to acis-acting regulatory element, a.k.a. cis-element, which confers anaspect of the overall expression pattern, but is usually insufficientalone to drive transcription, of an operably linked DNA sequence. Unlikepromoters, enhancer elements do not usually include a transcriptionstart site (TSS) or TATA box or equivalent DNA sequence. A promoter orpromoter fragment may naturally comprise one or more enhancer elementsthat affect the transcription of an operably linked DNA sequence. Anenhancer element may also be fused to a promoter to produce a chimericpromoter cis-element, which confers an aspect of the overall modulationof gene expression.

Regulatory elements may be characterized by their gene expressionpattern, e.g., positive and/or negative effects, such as constitutiveexpression or temporal, spatial, developmental, tissue, environmental,physiological, pathological, cell cycle, and/or chemically responsiveexpression, and any combination thereof, as well as by quantitative orqualitative indications. As used herein, a “gene expression pattern” isany pattern of transcription of an operably linked DNA molecule into atranscribed RNA molecule. The transcribed RNA molecule may be translatedto produce a protein molecule or may provide an antisense or otherregulatory RNA molecule, such as a double-stranded RNA (dsRNA), atransfer RNA (tRNA), a ribosomal RNA (rRNA), a microRNA (miRNA), and thelike.

As used herein, the term “protein expression” is any pattern oftranslation of a transcribed RNA molecule into a protein molecule.Protein expression may be characterized by its temporal, spatial,developmental, or morphological qualities, as well as by quantitative orqualitative indications.

A promoter is useful as a regulatory element for modulating theexpression of an operably linked transcribable DNA molecule. As usedherein, the term “promoter” refers generally to a DNA molecule that isinvolved in recognition and binding of RNA polymerase II and otherproteins, such as trans-acting transcription factors, to initiatetranscription. A promoter may originate from the 5′ untranslated region(5′ UTR) of a gene. Alternately, promoters may be synthetically producedor manipulated DNA molecules. Promoters may also be chimeric. As usedherein, the term “chimeric” refers to a single DNA molecule produced byfusing a first DNA molecule to a second DNA molecule, where neither thefirst nor the second DNA molecule would normally be contained in thatconfiguration, i.e., fused to the other. The chimeric DNA molecule isthus a new DNA molecule not otherwise normally contained in nature. Asused herein, the term “chimeric promoter” refers to a promoter producedthrough such manipulation of DNA molecules. A chimeric promoter maycombine two or more DNA fragments, for example, the fusion of a promoterto an enhancer element. Thus, the design, construction, and use ofchimeric promoters according to the methods disclosed herein formodulating the expression of operably linked transcribable DNA moleculesare encompassed by the disclosure.

In specific embodiments, chimeric DNA molecules and any variants orderivatives thereof as described herein, are further defined ascomprising promoter activity, i.e., are capable of acting as a promoterin a host cell, such as in a transgenic plant. In still further specificembodiments, a fragment may be defined as exhibiting promoter activitypossessed by the starting promoter molecule from which it is derived, ora fragment may comprise a “minimal promoter” which provides a basallevel of transcription and is comprised of a TATA box or equivalent DNAsequence for recognition and binding of the RNA polymerase II complexfor initiation of transcription.

Exemplary promoters for expression of a nucleic acid sequence includeplant promoters such as the CaMV 35S promoter, or others such as CaMV19S, nos, Adh, sucrose synthase, α-tubulin, actin, cab, PEPCase or thosepromoters associated with the R gene complex. Tissue-specific promoterssuch as leaf specific promoters, or tissue selective promoters (e.g.,promoters that direct greater expression in leaf primordia than in othertissues), and tissue-specific enhancers are also contemplated to beuseful, as are inducible promoters such as ABA- and turgor-induciblepromoters. Any suitable promoters known in the art may be used toexpress defensin or defensin-like coding sequences in a plant. In anembodiment, the CaMV35S promoter may be used to express defensin ordefensin-like coding sequences in a plant. In yet another embodiment, adisease or pathogen inducible promoter can be used to express defensinor defensin like proteins. Examples of disease or pathogen induciblepromoters can be found in Kooshki et al. Plant Science 165 (2003)213-219, Koschmann et al. Plant Physiology 160 (2012) 178-191, Rushtonet al. The Plant Cell, 14 (2002) 749-762, and Kirsch et al. The PlantJournal (2001) 26 217-227.

The DNA sequence between the transcription initiation site and the startof the coding sequence, i.e., the untranslated leader sequence, can alsoinfluence gene expression. As used herein, the term “leader” refers to aDNA molecule from the untranslated 5′ region (5′ UTR) of a gene anddefined generally as a DNA segment between the transcription start site(TSS) and the protein coding sequence start site. Alternately, leadersmay be synthetically produced or manipulated DNA elements. A leader canbe used as a 5′ regulatory element for modulating expression of anoperably linked transcribable DNA molecule. Leader molecules may be usedwith a heterologous promoter or with their native promoter. One may thuswish to employ a particular leader sequence with a transformationconstruct of the present disclosure. In an embodiment, leader sequencesare contemplated to include those which comprise sequences predicted todirect optimum expression of the attached gene, i.e., to include aconsensus leader sequence which may increase or maintain mRNA stabilityand prevent inappropriate initiation of translation. The choice of suchsequences will be known to those of skill in the art in light of thepresent disclosure. In some embodiments, sequences that are derived fromgenes that are highly expressed in plants may be used for expression ofdefensin or defensin-like coding sequences.

It is envisioned that defensin or defensin-like coding sequences may beintroduced under the control of novel promoters, enhancers, etc., orhomologous or tissue-specific or tissue-selective, or pathogen ordisease promoters or control elements. Vectors for use intissue-specific targeting of genes in transgenic plants will typicallyinclude tissue-specific or tissue-selective promoters and may alsoinclude other tissue-specific or tissue-selective control elements suchas enhancer sequences. Promoters which direct specific or enhancedexpression in certain plant tissues will be known to those of skill inthe art in light of the present disclosure.

Transformation constructs prepared in accordance with the presentdisclosure may further include a 3′ end DNA sequence that acts as asignal to terminate transcription and allow for the polyadenylation ofthe mRNA produced by coding sequences operably linked to a promoter. Asused herein, the term “3′ transcription termination molecule,” “3′untranslated region” or “3′ UTR” herein refers to a DNA molecule that isused during transcription to the untranslated region of the 3′ portionof an mRNA molecule. The 3′ untranslated region of an mRNA molecule maybe generated by specific cleavage and 3′ polyadenylation, also known asa polyA tail. A 3′ UTR may be operably linked to and located downstreamof a transcribable DNA molecule and may include a polyadenylation signaland other regulatory signals capable of affecting transcription, mRNAprocessing, or gene expression. PolyA tails are thought to function inmRNA stability and in initiation of translation. Examples of 3′transcription termination molecules in the art are the nopaline synthase3′ region; wheat hsp17 3′ region, pea rubisco small subunit 3′ region,cotton E6 3′ region, and the coixin 3′ UTR.

3′ UTRs typically find beneficial use for the recombinant expression ofspecific DNA molecules. A weak 3′ UTR has the potential to generateread-through, which may affect the expression of the DNA moleculelocated in the neighboring expression cassettes. Appropriate control oftranscription termination can prevent read-through into DNA sequences(e.g., other expression cassettes) localized downstream and can furtherallow efficient recycling of RNA polymerase to improve gene expression.Efficient termination of transcription (release of RNA Polymerase IIfrom the DNA) is prerequisite for re-initiation of transcription andthereby directly affects the overall transcript level. Subsequent totranscription termination, the mature mRNA is released from the site ofsynthesis and template transported to the cytoplasm. Eukaryotic mRNAsare accumulated as poly(A) forms in vivo, making it difficult to detecttranscriptional termination sites by conventional methods. However,prediction of functional and efficient 3′ UTRs by bioinformatics methodsis difficult in that there are no conserved DNA sequences that wouldallow easy prediction of an effective 3′ UTR. In one embodiment, thenative terminator of a defensin or defensin-like coding sequence may beused. Alternatively, a heterologous 3′ end may enhance the expression ofsense or antisense defensin or defensin-like coding sequences.

Sequences that are joined to the coding sequence of an expressed gene,which are removed post-translationally from the initial translationproduct and which facilitate the transport of the protein into orthrough intracellular or extracellular membranes, are termed transit ortargeting peptide (usually into vacuoles, vesicles, plastids and otherintracellular organelles) and signal peptide or sequences (usually tothe endoplasmic reticulum, Golgi apparatus, and outside of the cellularmembrane). By facilitating the transport of the protein intocompartments inside and outside the cell, these sequences may increasethe accumulation of gene products by protecting them from proteolyticdegradation. These sequences also allow for additional mRNA sequencesfrom highly expressed genes to be attached to the coding sequence of thegenes. Since mRNA being translated by ribosomes is more stable thannaked mRNA, the presence of translatable mRNA in front of the gene mayincrease the overall stability of the mRNA transcript from the gene andthereby increase synthesis of the gene product. Since transit and signalsequences are usually post-translationally removed from the initialtranslation product, the use of these sequences allows for the additionof extra translated sequences that may not appear on the finalpolypeptide. It further is contemplated that targeting of certainproteins may be desirable in order to enhance the stability of theprotein.

Additionally, vectors may be constructed and employed in theintracellular targeting of a specific gene product within the cells of atransgenic plant or in directing a protein to the extracellularenvironment. This generally will be achieved by joining a DNA sequenceencoding a transit or signal peptide sequence to the coding sequence ofa particular gene. The resultant transit or signal peptide willtransport the protein to a particular intracellular or extracellulardestination, respectively, and will then be post-translationallyremoved.

By employing a selectable or screenable marker, one can provide orenhance the ability to identify transformants. “Marker genes” are genesthat impart a distinct phenotype to cells expressing the marker proteinand thus allow such transformed cells to be distinguished from cellsthat do not have the marker. Such genes may encode either a selectableor screenable marker, depending on whether the marker confers a traitwhich one can “select” for by chemical means, i.e., through the use of aselective agent (e.g., a herbicide, antibiotic, or the like), or whetherit is simply a trait that one can identify through observation ortesting, i.e., by “screening” (e.g., the green fluorescent protein). Ofcourse, many examples of suitable marker proteins are known to the artand can be employed in the practice of the present disclosure.

Selectable marker transgenes may also be used with the presentdisclosure. As used herein the term “selectable marker transgene” refersto any transcribable DNA molecule whose expression in a transgenicplant, tissue or cell, or lack thereof, can be screened for or scored insome way. Selectable marker genes, and their associated selection andscreening techniques, for use in the practice of the present disclosureare known in the art and include, but are not limited to, transcribableDNA molecules encoding B-glucuronidase (GUS), green fluorescent protein(GFP), proteins that confer antibiotic resistance, and proteins thatconfer herbicide tolerance.

VII. Plant Cell Transformation Methods

Numerous methods for transforming chromosomes in a plant cell withrecombinant DNA are known in the art and are used in methods ofproducing a transgenic plant cell and plant. Two effective methods forsuch transformation are Agrobacterium-mediated transformation andmicroprojectile bombardment-mediated transformation. Microprojectilebombardment methods are illustrated in U.S. Pat. No. 5,015,580(soybean); U.S. Pat. No. 5,550,318 (corn); U.S. Pat. No. 5,538,880(corn); U.S. Pat. No. 5,914,451 (soybean); U.S. Pat. No. 6,160,208(corn); U.S. Pat. No. 6,399,861 (corn); U.S. Pat. No. 6,153,812 (wheat)and U.S. Pat. No. 6,365,807 (rice). Agrobacterium-mediatedtransformation methods are described in U.S. Pat. No. 5,159,135(cotton); U.S. Pat. No. 5,824,877 (soybean); U.S. Pat. No. 5,463,174(canola); U.S. Pat. No. 5,591,616 (corn); U.S. Pat. No. 5,846,797(cotton); U.S. Pat. No. 6,384,301 (soybean), U.S. Pat. No. 7,026,528(wheat) and U.S. Pat. No. 6,329,571 (rice), and US Patent ApplicationPublication Nos. US 2004/0087030 A1 (cotton), and US 2001/0042257 A1(sugar beet), all of which are incorporated herein by reference in theirentirety. Transformation of plant material is practiced in tissueculture on nutrient media, for example a mixture of nutrients that allowcells to grow in vitro. Recipient cell targets include, but are notlimited to, meristem cells, shoot tips, hypocotyls, calli, immature ormature embryos, and gametic cells such as microspores, pollen, sperm andegg cells. Callus can be initiated from tissue sources including, butnot limited to, immature or mature embryos, hypocotyls, seedling apicalmeristems, microspores and the like. Cells containing a transgenicnucleus are grown into transgenic plants.

In addition to direct transformation of a plant material with arecombinant DNA, a transgenic plant can be prepared by crossing a firstplant comprising a recombinant DNA with a second plant lacking therecombinant DNA. For example, recombinant DNA can be introduced into afirst plant line that is amenable to transformation, which can becrossed with a second plant line to introgress the recombinant DNA intothe second plant line. A transgenic plant with recombinant DNA providingan enhanced trait, for example, enhanced yield, can be crossed with atransgenic plant line having another recombinant DNA that confersanother trait, for example herbicide resistance or pest resistance orenhanced water use efficiency, to produce progeny plants havingrecombinant DNA that confers both traits. Typically, in such breedingfor combining traits the transgenic plant donating the additional traitis the male line and the transgenic plant carrying the base traits isthe female line. The progeny of this cross will segregate such that someof the plants will carry the DNA for both parental traits and some willcarry DNA for one parental trait; such plants can be identified bymarkers associated with parental recombinant DNA, for example, markeridentification by analysis for recombinant DNA or, in the case where aselectable marker is linked to the recombinant DNA, by application usinga selective agent such as a herbicide for use with a herbicide tolerancemarker, or by selection for the enhanced trait. Progeny plants carryingDNA for both parental traits can be crossed back into the female parentline multiple times, for example usually 6 to 8 generations, to producea progeny plant with substantially the same genotype as the originaltransgenic parental line but for the recombinant DNA of the othertransgenic parental line.

In transformation, DNA is typically introduced into only a smallpercentage of target plant cells in any one transformation experiment.Marker genes are used to provide an efficient system for identificationof those cells that are stably transformed by receiving and integratinga recombinant DNA molecule into their genomes. Preferred marker genesprovide selective markers which confer resistance to a selective agent,such as an antibiotic or an herbicide. Any of the herbicides to whichplants of this disclosure can be resistant is an agent for selectivemarkers. Potentially transformed cells are exposed to the selectiveagent. In the population of surviving cells are those cells where,generally, the resistance-conferring gene is integrated and expressed atsufficient levels to permit cell survival. Cells can be tested furtherto confirm stable integration of the exogenous DNA. Commonly usedselective marker genes include those conferring resistance toantibiotics such as kanamycin and paromomycin (nptII), hygromycin B (aphIV), spectinomycin (aadA) and gentamycin (aac3 and aacC4) or resistanceto herbicides such as glufosinate (bar or pat), dicamba (DMO) andglyphosate (aroA or EPSPS). Examples of such selectable markers areillustrated in U.S. Pat. No. 5,550,318; U.S. Pat. No. 5,633,435; U.S.Pat. No. 5,780,708 and U.S. Pat. No. 6,118,047. Markers which provide anability to visually screen transformants can also be employed, forexample, a gene expressing a colored or fluorescent protein such as aluciferase or green fluorescent protein (GFP) or a gene expressing abeta-glucuronidase or uidA gene (GUS) for which various chromogenicsubstrates are known.

VIII. Transgenic Plants and Seeds

Transgenic plants derived from transgenic plant cells having atransgenic nucleus of this disclosure are grown to generate transgenicplants having an enhanced trait as compared to a control plant, andproduce transgenic seed and haploid pollen of this disclosure. Suchplants with enhanced traits are identified by selection of transformedplants or progeny seed for the enhanced trait. For efficiency aselection method is designed to evaluate multiple transgenic plants(events) comprising the recombinant DNA, for example multiple plantsfrom 2 to 20 or more transgenic events. Transgenic plants grown fromtransgenic seeds provided herein demonstrate improved agronomic traits,such as resistance to Fusarium stalk rot in maize.

IX. General Terms and Definitions

Systems, including automated systems for selecting plants that comprisea marker of interest and/or for correlating presence of the marker withresistance are also a feature of the invention. These systems caninclude probes relevant to marker locus detection, detectors fordetecting labels on the probes, appropriate fluid handling elements andtemperature controllers that mix probes and templates and/or amplifytemplates and systems instructions that correlate label detection to thepresence of a particular marker locus or allele.

In an aspect, this invention could be used on any plant. In anotheraspect, the plant is selected from the genus Zea. In another aspect, theplant is selected from the species Zea mays. In a further aspect, theplant is selected from the subspecies Zea mays L. ssp. mays. In anadditional aspect, the plant is selected from the group Zea mays L.subsp. mays Indentata, otherwise known as dent corn. In another aspect,the plant is selected from the group Zea mays L. subsp. mays Indurata,otherwise known as flint corn. In an aspect, the plant is selected fromthe group Zea mays L. subsp. mays Saccharata, otherwise known as sweetcorn. In another aspect, the plant is selected from the group Zea maysL. subsp. mays Amylacea, otherwise known as flour corn. In a furtheraspect, the plant is selected from the group Zea mays L. subsp. maysEverta, otherwise known as pop corn. Zea plants include hybrids,inbreds, partial inbreds, or members of defined or undefinedpopulations.

In a preferred aspect, the present invention provides a plant to beassayed for resistance or susceptibility to disease by any method todetermine whether a plant is resistant, susceptible, or whether itexhibits some degree of resistance or susceptibility. Populations ofplants can be similarly characterized in this manner, or furthercharacterized as segregating for the trait of disease resistance.

It is further understood that a plant of the present invention mayexhibit the characteristics of any relative maturity group. In anaspect, the maturity group is selected from the group consisting ofearly maturing varieties, mid season maturing varieties, and full seasonvarieties.

The present invention also provides for parts of the plants of thepresent invention. Plant parts, without limitation, include seed,endosperm, ovule and pollen. In a particularly preferred aspect of thepresent invention, the plant part is a seed.

In another aspect, the corn seed can be subjected to various treatments.For example, the seeds can be treated to improve germination by primingthe seeds or by disinfection to protect against seed-born pathogens. Inanother aspect, seeds can be coated with any available coating toimprove, for example, plantability, seed emergence, and protectionagainst seed-born pathogens. Seed coating can be any form of seedcoating including, but not limited to, pelleting, film coating, andencrustments.

In another aspect, the corn plant can show a comparative resistancecompared to a non-resistant control corn plant. In this aspect, acontrol corn plant will preferably be genetically similar except for thedisease resistance allele or alleles in question. Such plants can begrown under similar conditions with equivalent or near equivalentexposure to the pathogen.

Various patent and non-patent publications are cited herein, thedisclosures of each of which are incorporated herein by reference intheir entireties.

As various modifications could be made in the constructions and methodsherein described and illustrated without departing from the scope of theinvention, it is intended that all matter contained in the foregoingdescription or shown in the accompanying drawings shall be interpretedas illustrative rather than limiting. The breadth and scope of thepresent invention should not be limited by any of the above-describedexemplary embodiments, but should be defined only in accordance with thefollowing claims appended hereto and their equivalents.

Descriptions of commonly used breeding terms and methods for crossingand producing hybrids that are used to describe present invention can befound in one of several reference books (Allard, “Principles of PlantBreeding,” John Wiley & Sons, NY, U. of CA, Davis, Calif., 50-98, 1960;Simmonds, “Principles of crop improvement,” Longman, Inc., NY, 369-399,1979; Sneep and Hendriksen, “Plant breeding perspectives,” Wageningen(ed), Center for Agricultural Publishing and Documentation, 1979; Fehr,In: Soybeans: Improvement, Production and Uses, 2nd Edition, Monograph.,16:249, 1987; Fehr, “Principles of variety development,” Theory andTechnique, (Vol. 1) and Crop Species Soybean (Vol. 2), Iowa State Univ.,Macmillan Pub. Co., NY, 360-376, 1987).

The definitions and methods provided define the present invention andguide those of ordinary skill in the art in the practice of the presentinvention. Unless otherwise noted, terms are to be understood accordingto conventional usage by those of ordinary skill in the relevant art.Examples of resources describing many of the terms related to molecularbiology used herein can be found in in Alberts et al., Molecular Biologyof The Cell, 5^(th) Edition, Garland Science Publishing, Inc.: New York,2007; Rieger et al., Glossary of Genetics: Classical and Molecular, 5thedition, Springer-Verlag: New York, 1991; King et al, A Dictionary ofGenetics, 6th ed, Oxford University Press: New York, 2002; and Lewin,Genes Icorn, Oxford University Press New York, 2007. The nomenclaturefor DNA bases as set forth at 37 CFR §1.822 is used.

DEFINITIONS

Terms defined herein have meanings as commonly understood by a person ofordinary skill in the areas relevant to the present invention. Termssuch as “a”, “an” and “the” are not intended to refer to only a singularentity, but include the general class of which a specific example may beused for illustration. The terminology herein is used to describespecific embodiments of the invention, but their usage does not delimitthe invention, except as outlined in the claims.

“Adjacent”, when used to describe a nucleic acid molecule thathybridizes to DNA containing a polymorphism, refers to a nucleic acidthat hybridizes to DNA sequences that directly abut the polymorphicnucleotide base position. For example, a nucleic acid molecule that canbe used in a single base extension assay is “adjacent” to thepolymorphism.

“Allele” generally refers to an alternative nucleic acid sequence at aparticular locus; the length of an allele can be as small as 1nucleotide base, but is typically larger. For example, a first allelecan occur on one chromosome, while a second allele occurs on a secondhomologous chromosome, e.g., as occurs for different chromosomes of aheterozygous individual, or between different homozygous or heterozygousindividuals in a population. A favorable allele is the allele at aparticular locus that confers, or contributes to, an agronomicallydesirable phenotype, or alternatively, is an allele that allows theidentification of susceptible plants that can be removed from a breedingprogram or planting. A favorable allele of a marker is a marker allelethat segregates with the favorable phenotype, or alternatively,segregates with susceptible plant phenotype, therefore providing thebenefit of identifying disease prone plants. A favorable allelic form ofa chromosome interval is a chromosome interval that includes anucleotide sequence that contributes to superior agronomic performanceat one or more genetic loci physically located on the chromosomeinterval. “Allele frequency” refers to the frequency (proportion orpercentage) at which an allele is present at a locus within anindividual, within a line, or within a population of lines. For example,for an allele “A,” diploid individuals of genotype “AA,” “Aa,” or “aa”have allele frequencies of 1.0, 0.5, or 0.0, respectively. One canestimate the allele frequency within a line by averaging the allelefrequencies of a sample of individuals from that line. Similarly, onecan calculate the allele frequency within a population of lines byaveraging the allele frequencies of lines that make up the population.For a population with a finite number of individuals or lines, an allelefrequency can be expressed as a count of individuals or lines (or anyother specified grouping) containing the allele. An allele positivelycorrelates with a trait when it is linked to it and when presence of theallele is an indictor that the desired trait or trait form will occur ina plant comprising the allele. An allele negatively correlates with atrait when it is linked to it and when presence of the allele is anindicator that a desired trait or trait form will not occur in a plantcomprising the allele.

“Crossed” or “cross” means to produce progeny via fertilization (e.g.cells, seeds or plants) and includes crosses between plants (sexual) andself fertilization (selfing).

“Elite line” means any line that has resulted from breeding andselection for superior agronomic performance. Numerous elite lines areavailable and known to those of skill in the art of corn breeding. An“elite population” is an assortment of elite individuals or lines thatcan be used to represent the state of the art in terms of agronomicallysuperior genotypes of a given crop species, such as corn. Similarly, an“elite germplasm” or elite strain of germplasm is an agronomicallysuperior germplasm, typically derived from and/or capable of giving riseto a plant with superior agronomic performance, such as an existing ornewly developed elite line of corn. In contrast, an “exotic line” or“exotic germplasm” is a line or germplasm derived from a plant notbelonging to an available elite line or strain of germplasm. In thecontext of a cross between two plants or lines of germplasm, an exoticgermplasm is not closely related by descent to the elite germplasm withwhich it is crossed. Most commonly, the exotic germplasm is not derivedfrom any known elite line of a crop, but rather is selected to introducegenetic elements (typically desired alleles) into a breeding program.

“Exogenous nucleic acid” is a nucleic acid that is not native to aspecified system (e.g., a germplasm, plant, variety, etc.), with respectto sequence, genomic position, or both. As used herein, the terms“exogenous” or “heterologous” as applied to polynucleotides orpolypeptides typically refers to molecules that have been artificiallysupplied to a biological system (e.g., a plant cell, a plant gene, aparticular plant species or variety or a plant chromosome under study)and are not native to that particular biological system. The terms canindicate that the relevant material originated from a source other thana naturally occurring source, or can refer to molecules having anon-natural configuration, genetic location or arrangement of parts. Incontrast, for example, a “native” or “endogenous” gene is a gene thatdoes not contain nucleic acid elements encoded by sources other than thechromosome or other genetic element on which it is normally found innature. An endogenous gene, transcript or polypeptide is encoded by itsnatural chromosomal locus, and not artificially supplied to the cell.

“Genetic element” or “gene” refers to a heritable sequence of DNA, i.e.,a genomic sequence, with functional significance. The term “gene” canalso be used to refer to, e.g., a cDNA and/or a mRNA encoded by agenomic sequence, as well as to that genomic sequence.

“Genotype” is the genetic constitution of an individual (or group ofindividuals) at one or more genetic loci, as contrasted with theobservable trait (the phenotype). Genotype is defined by the allele(s)of one or more known loci that the individual has inherited from itsparents. The term genotype can be used to refer to an individual'sgenetic constitution at a single locus, at multiple loci, or, moregenerally, the term genotype can be used to refer to an individual'sgenetic make-up for all the genes in its genome. A “haplotype” is thegenotype of an individual at a plurality of genetic loci. Typically, thegenetic loci described by a haplotype are physically and geneticallylinked, i.e., on the same chromosome interval. The terms “phenotype,” or“phenotypic trait” or “trait” refers to one or more trait of anorganism. The phenotype can be observable to the naked eye, or by anyother means of evaluation known in the art, e.g., microscopy,biochemical analysis, genomic analysis, an assay for a particulardisease resistance, etc. In some cases, a phenotype is directlycontrolled by a single gene or genetic locus, i.e., a “single genetrait.” In other cases, a phenotype is the result of several genes.

“Germplasm” refers to genetic material of or from an individual (e.g., aplant), a group of individuals (e.g., a plant line, variety or family),or a clone derived from a line, variety, species, or culture. Thegermplasm can be part of an organism or cell, or can be separate fromthe organism or cell. In general, germplasm provides genetic materialwith a specific molecular makeup that provides a physical foundation forsome or all of the hereditary qualities of an organism or cell culture.As used herein, germplasm includes cells, seed or tissues from which newplants may be grown, or plant parts, such as leafs, stems, pollen, orcells that can be cultured into a whole plant.

“Linkage disequilibrium” or “LD” refers to a non-random segregation ofgenetic loci or traits (or both). In either case, linkage disequilibriumimplies that the relevant loci are within sufficient physical proximityalong a length of a chromosome so that they segregate together withgreater than random (i.e., non-random) frequency (in the case ofco-segregating traits, the loci that underlie the traits are insufficient proximity to each other). Linked loci co-segregate more than50% of the time, e.g., from about 51% to about 100% of the time. Theterm “physically linked” is sometimes used to indicate that two loci,e.g., two marker loci, are physically present on the same chromosome.Advantageously, the two linked loci are located in close proximity suchthat recombination between homologous chromosome pairs does not occurbetween the two loci during meiosis with high frequency, e.g., such thatlinked loci cosegregate at least about 90% of the time, e.g., 91%, 92%,93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.75%, or more of the time.

“Locus” a chromosome region where a polymorphic nucleic acid, traitdeterminant, gene or marker is located. The loci of this inventioncomprise one or more polymorphisms in a population; i.e., alternativealleles are present in some individuals. A “gene locus” is a specificchromosome location in the genome of a species where a specific gene canbe found.

“Marker Assay” means a method for detecting a polymorphism at aparticular locus using a particular method, e.g. measurement of at leastone phenotype (such as seed color, flower color, or other visuallydetectable trait), restriction fragment length polymorphism (RFLP),single base extension, electrophoresis, sequence alignment, allelicspecific oligonucleotide hybridization (ASO), random amplifiedpolymorphic DNA (RAPD), microarray-based technologies, and nucleic acidsequencing technologies, etc. “Marker Assisted Selection” (MAS) is aprocess by which phenotypes are selected based on marker genotypes.

“Molecular phenotype” is a phenotype detectable at the level of apopulation of one or more molecules. Such molecules can be nucleicacids, proteins, or metabolites. A molecular phenotype could be anexpression profile for one or more gene products, e.g., at a specificstage of plant development, in response to an environmental condition orstress, etc.

“Operably linked” refers to the association of two or more nucleic acidelements in a recombinant DNA construct, e.g. as when a promoter isoperably linked with DNA that is transcribed to RNA whether forexpressing or suppressing a protein. Recombinant DNA constructs can bedesigned to express a protein which can be an endogenous protein, anexogenous homologue of an endogenous protein or an exogenous proteinwith no native homologue. Alternatively, recombinant DNA constructs canbe designed to suppress the level of an endogenous protein, e.g. bysuppression of the native gene. Such gene suppression can be effectivelyemployed through a native RNA interference (RNAi) mechanism in whichrecombinant DNA comprises both sense and anti-sense oriented DNA matchedto the gene targeted for suppression where the recombinant DNA istranscribed into RNA that can form a double-strand to initiate an RNAimechanism. Gene suppression can also be effected by recombinant DNA thatcomprises anti-sense oriented DNA matched to the gene targeted forsuppression. Gene suppression can also be effected by recombinant DNAthat comprises DNA that is transcribed to a microRNA matched to the genetargeted for suppression.

“Percent identity” or “% identity” means the extent to which twooptimally aligned DNA or protein segments are invariant throughout awindow of alignment of components, for example nucleotide sequence oramino acid sequence. An “identity fraction” for aligned segments of atest sequence and a reference sequence is the number of identicalcomponents that are shared by sequences of the two aligned segmentsdivided by the total number of sequence components in the referencesegment over a window of alignment which is the smaller of the full testsequence or the full reference sequence.

“Phenotype” means the detectable characteristics of a cell or organismwhich can be influenced by genotype.

“Plant” refers to a whole plant any part thereof, or a cell or tissueculture derived from a plant, comprising any of: whole plants, plantcomponents or organs (e.g., leaves, stems, roots, etc.,), plant tissues,seeds, plant cells, and/or progeny of the same. A plant cell is abiological cell of a plant, taken from a plant or derived throughculture from a cell taken from a plant.

“Polymorphism” means the presence of one or more variations in apopulation. A polymorphism may manifest as a variation in the nucleotidesequence of a nucleic acid or as a variation in the amino acid sequenceof a protein. Polymorphisms include the presence of one or morevariations of a nucleic acid sequence or nucleic acid feature at one ormore loci in a population of one or more individuals. The variation maycomprise but is not limited to one or more nucleotide base changes, theinsertion of one or more nucleotides or the deletion of one or morenucleotides. A polymorphism may arise from random processes in nucleicacid replication, through mutagenesis, as a result of mobile genomicelements, from copy number variation and during the process of meiosis,such as unequal crossing over, genome duplication and chromosome breaksand fusions. The variation can be commonly found or may exist at lowfrequency within a population, the former having greater utility ingeneral plant breeding and the latter may be associated with rare butimportant phenotypic variation. Useful polymorphisms may include singlenucleotide polymorphisms (SNPs), insertions or deletions in DNA sequence(Indels), simple sequence repeats of DNA sequence (SSRs), a restrictionfragment length polymorphism, and a tag SNP. A genetic marker, a gene, aDNA-derived sequence, a RNA-derived sequence, a promoter, a 5′untranslated region of a gene, a 3′ untranslated region of a gene,microRNA, siRNA, a resistance locus, a satellite marker, a transgene,mRNA, ds mRNA, a transcriptional profile, and a methylation pattern mayalso comprise polymorphisms. In addition, the presence, absence, orvariation in copy number of the preceding may comprise polymorphisms.

A “population of plants” or “plant population” means a set comprisingany number, including one, of individuals, objects, or data from whichsamples are taken for evaluation, e.g. estimating QTL effects. Mostcommonly, the terms relate to a breeding population of plants from whichmembers are selected and crossed to produce progeny in a breedingprogram. A population of plants can include the progeny of a singlebreeding cross or a plurality of breeding crosses, and can be eitheractual plants or plant derived material, or in silico representations ofthe plants. The population members need not be identical to thepopulation members selected for use in subsequent cycles of analyses orthose ultimately selected to obtain final progeny plants. Often, a plantpopulation is derived from a single biparental cross, but may alsoderive from two or more crosses between the same or different parents.Although a population of plants may comprise any number of individuals,those of skill in the art will recognize that plant breeders commonlyuse population sizes ranging from one or two hundred individuals toseveral thousand, and that the highest performing 5-20% of a populationis what is commonly selected to be used in subsequent crosses in orderto improve the performance of subsequent generations of the population.

“Resistance” or “improved resistance” in a plant to disease conditionsis an indication that the plant is more able to reduce disease burdenthan a non-resistant or less resistant plant. Resistance is a relativeterm, indicating that a “resistant” plant is more able to reduce diseaseburden compared to a different (less resistant) plant (e.g., a differentcorn line) grown in similar disease conditions. One of skill willappreciate that plant resistance to disease conditions varies widely,and can represent a spectrum of more-resistant or less-resistantphenotypes. However, by simple observation, one of skill can generallydetermine the relative resistance of different plants, plant lines, orplant families under disease conditions, and furthermore, will alsorecognize the phenotypic gradations of “resistant.”

“Resistance locus” means a locus that contributes resistance, tolerance,or susceptibility to Fusarium stalk rot.

“Resistance allele” means the nucleic acid sequence associated withresistance or tolerance to disease.

“Recombinant” in reference to a nucleic acid or polypeptide indicatesthat the material (e.g., a recombinant nucleic acid, gene,polynucleotide, polypeptide, etc.) has been altered by humanintervention. The term recombinant can also refer to an organism thatharbors recombinant material, e.g., a plant that comprises a recombinantnucleic acid is considered a recombinant plant.

“Tolerance” or “improved tolerance” in a plant to disease conditions isan indication that the plant is less affected by disease conditions withrespect to yield, survivability and/or other relevant agronomicmeasures, compared to a less resistant, more “susceptible” plant.Tolerance is a relative term, indicating that a “tolerant” plantsurvives and/or produces better yields in disease conditions compared toa different (less tolerant) plant (e.g., a different corn line strain)grown in similar disease conditions. One of skill will appreciate thatplant tolerance to disease conditions varies widely, and can represent aspectrum of more-tolerant or less-tolerant phenotypes. However, bysimple observation, one of skill can generally determine the relativetolerance or susceptibility of different plants, plant lines or plantfamilies under disease conditions, and furthermore, will also recognizethe phenotypic gradations of “tolerant.”

“Transgenic plant” refers to a plant that comprises within its cells aheterologous polynucleotide. Generally, the heterologous polynucleotideis stably integrated within the genome such that the polynucleotide ispassed on to successive generations. The heterologous polynucleotide maybe integrated into the genome alone or as part of a recombinantexpression cassette. “Transgenic” is used herein to refer to any cell,cell line, callus, tissue, plant part or plant, the genotype of whichhas been altered by the presence of heterologous nucleic acid includingthose transgenic organisms or cells initially so altered, as well asthose created by crosses or asexual propagation from the initialtransgenic organism or cell. The term “transgenic” as used herein doesnot encompass the alteration of the genome (chromosomal orextrachromosomal) by conventional plant breeding methods (e.g., crosses)or by naturally occurring events such as random cross-fertilization,non-recombinant viral infection, non-recombinant bacterialtransformation, non-recombinant transposition, or spontaneous mutation.

“Vector” is a polynucleotide or other molecule that transfers nucleicacids between cells. Vectors are often derived from plasmids,bacteriophages, or viruses and optionally comprise parts which mediatevector maintenance and enable its intended use. A “cloning vector” or“shuttle vector” or “sub cloning vector” contains operably linked partsthat facilitate subcloning steps (e.g., a multiple cloning sitecontaining multiple restriction endonuclease sites). The term“expression vector” as used herein refers to a vector comprisingoperably linked polynucleotide sequences that facilitate expression of acoding sequence in a particular host organism (e.g., a bacterialexpression vector or a plant expression vector).

“Yield” is the culmination of all agronomic traits as determined by theproductivity per unit area of a particular plant product of commercialvalue. “Agronomic traits,” include the underlying genetic elements of agiven plant variety that contribute to yield over the course of growingseason.

EXAMPLES Example 1 Inoculation and Assessment of FSR ResistancePhenotypes

Corn plants were inoculated 10 days after flowering by injecting aninternode of each stalk with 2×10⁵ Fusarium moniliforme spores suspendedin 2 mL of distilled water. Six weeks after inoculation, the severity ofFusarium stalk rot in each plant was visually assessed by splittingstalks longitudinally to expose the pith. Internodes at the site ofinoculation were examined to determine the percent of the internodeshowing visual lesions characteristic of the disease (necrosis), assummarized in Table 2. The individual plant scores of each row were thenaveraged to generate a final score for the row.

TABLE 2 Rating Scale of relative FSR infection resistance phenotypes. %Internode Necrosis Score Rating  0-10% 1 Highly resistant 10-20% 2Highly resistant 20-30% 3 Resistant 30-40% 4 Resistant 40-50% 5Intermediate 50-60% 6 Susceptible 60-70% 7 Susceptible >80% 8 Highlysusceptible >80% and necrosis expands 9 Highly susceptible to adjacentinternode

Example 2 Assays Useful for Detecting FSR Resistance Genotypes

For convenience, primer sequences for amplifying SNP marker loci linkedto FSR-3.01 or FSR-8.01 and the probes used to genotype thecorresponding SNP sequences are provided in Table 3. The SNP positionwithin the SEQ ID NO. is given in the second column. Primer and probesynthesis is within the skill of the art once the SNP position in thecorn genome is provided. One of skill in the art will also immediatelyrecognize that other sequences to either side of the given primers canbe used in place of the given primers, so long as the primers canamplify a region that includes the allele to be detected. Further, itwill be appreciated that the precise probe to be used for detection canvary, e.g., any probe that can identify the region of a marker ampliconto be detected can be substituted for those examples provided herein.Also, configuration of the amplification primers and detection probescan, of course, vary. Thus, the invention is not limited to the primers,probes, or marker sequences specifically recited herein.

TABLE 3 Primers and probes useful for detecting FSR resistance. SEQ IDNO. SEQ ID SNP Fwd Rev NO. Pos Primer Primer Probe 1 Probe 2 1 81 18 3552 69 2 46 19 36 53 70 3 49 20 37 54 71 4 319 21 38 55 72 5 96 22 39 5673 6 180 23 40 57 74 7 263 24 41 58 75 8 132 25 42 59 76 9 538 26 43 6077 10 49 27 44 61 78 11 139 28 45 62 79 12 101 29 46 63 80 13 551 30 4764 81 14 101 31 48 65 82 15 968 32 49 66 83 16 58 33 50 67 84 17 101 3451 68 85

Illustrative FSR resistance marker DNA sequences SEQ ID NOs: 1, 5, or 14can be amplified using the primers indicated in Table 3 using SEQ IDNOs: 18 and 35, 22 and 39, or 31 and 48, respectively, and detected withprobes indicated in Table 3 as SEQ ID NOs: 52 and 69, 56 and 73, or 65and 82, respectively.

Example 3 Marker-Trait Association Study

Two replicates of F2-derived F3 individuals and F4 individuals derivedfrom F3 heterozygotes by the cross between a resistant inbred maize lineand a susceptible inbred maize line were inoculated and phenotyped forFSR resistance at La Charca and Tlajomulco, Mexico using methodsdescribed in Example 1.

DNA was also extracted from each individual in each replicate at eachlocation and genotyped with over 100 SNP markers that were selected tocollectively span the maize genome (Table 4). Loci were eliminated fromfurther analysis where they were monomorphic in the subject populationstudied.

Marker-trait association studies were performed using both single-markeranalysis (SMA) and composite interval mapping (CIM). For each marker,the thresholds of Likelihood ratio between full and null models for CIMwere based on 1000 permutation tests and the thresholds (p-value) forSMA were based on 10,000 permutation tests (Churchill and Doerge 1994).

TABLE 4 Two replicates of two independent populations derived from FSRresistant and FSR susceptible parents were inoculated with FSR spores,phenotyped for FSR resistance, and genotyped with either 141 or 144 SNPmarkers. No. of No. of Population Location Individuals SNP 1 La Charca,Tlajomulco 299 141 2 La Charca, Tlajomulco 179 144

Detection of FSR-3.01

Table 5 lists the effect estimates on FSR resistance phenotype ratingsassociated with markers that revealed significant associations withFSR-3.01. Each row provides the SEQ ID NO. of the marker, and theestimated effect that the marker polymorphism had on the FSR phenotype.The statistical significance (p-value) of the association between themarker and the FSR resistance rating in each case was p-val≦0.001.

TABLE 5 Effect estimates on FSR ratings of example markers associatedwith FSR-3.01. SEQ ID NO. Effect Estimate 1 0.56 2 0.67 3 0.2 4 0.55 50.52

For example, SEQ ID NO: 1 was associated with a 0.56 change in FSRresistance rating by one copy of the favorable allele. SEQ ID NO: 3 wasassociated with a 0.2 change in FSR resistance rating by one copy of thefavorable allele. FSR resistance ratings were generated using themethods described in Example 1.

Table 6 describes the chromosome 3 profile of the FSR-3.01 QTL revealedby the CIM analysis, including the chromosome interval where theLikelihood ratio was within the threshold of p-value≦0.01.

TABLE 6 Results of the composite interval mapping (CIM) analysis onchromosome 3. SEQ ID NO: 1 and SEQ ID NO: 5 mark the ends of the regionof the chromosome where the CIM Likelihood ratio remained within thethreshold of p-value ≦0.01. The peak of the Likelihood ratio correspondsto the FSR-3.01 locus. CIM Profile Position Locus/Marker Position† CIMProfile on MON Locus/Nearest MON Map IBM2 Map Feature Map (cM) Marker(cM) (IcM) Left Border 102.2 SEQ ID NO: 1 102.2 352.2 Likelihood 112.9FSR-3.01 112.9 392.8 ratio peak Right Border 122.8 SEQ ID NO: 5 122.8426.3 †cM = centiMorgans, IcM = map units of the IBM2 2008 NeighborsGenetic Map.

Thus, the CIM analysis revealed that markers within the interval flankedby and including markers SEQ ID NO: 1 and SEQ ID NO: 5 were highlyassociated with FSR-3.01 (p-value≦0.01). Markers bordering FSR-3.01 alsofind utility with this invention, but their associations with thatinterval tend to decrease as their locations become further removed fromFSR-3.01.

Detection of FSR-8.01

Table 7 lists the effect estimates on FSR resistance phenotype ratingsassociated with markers that revealed significant associations withFSR-8.01. Each row provides the SEQ ID NO. of the marker, and theestimated effect that the marker polymorphism had on the FSR phenotype.The statistical significance (p-value) of the association between themarker and the FSR resistance rating in each case was p-val≦0.001.

TABLE 7 Effect estimates on FSR ratings of example markers associatedwith FSR-8.01. SEQ ID NO. Effect Estimate 7 1.177 8 1.181 9 1.237 101.161 11 1.142 12 1.175 13 1.19 14 1.172 15 1.202 16 1.109 17 1.141

For example, SEQ ID NO: 9 was associated with a 1.237 change in FSRresistance rating by one copy of the favorable allele. SEQ ID NO: 14 wasassociated with a 1.172 change in FSR resistance rating by one copy ofthe favorable allele. FSR resistance ratings were generated using themethods described in Example 1.

Table 8 describes the chromosome 8 profile of the FSR-8.01 QTL revealedby the CIM analysis, including the chromosome interval where theLikelihood ratio was within the threshold of p-value≦0.01.

TABLE 8 Results of the composite interval mapping (CIM) analysis onchromosome 8. SEQ ID NO: 6 and SEQ ID NO: 17 mark the ends of the regionof the chromosome where the CIM Likelihood ratio remained within thethreshold of p-value ≦0.01. The peak of the Likelihood ratio correspondsto the FSR-8.01 locus. CIM Profile Position Locus/Marker Position† CIMProfile on MON Locus/Nearest MON Map IBM2 Map Feature Map (cM) Marker cMIcM Left Boarder 69.0 SEQ ID NO: 6 69.0 233.6 Likelihood 71.0 FSR-8.0171.0 214.9 ratio peak Right 81.7 SEQ ID 81.7 285.3 Boarder NO: 17 †cM =centiMorgans, IcM = map units of the IBM2 2008 Neighbors Genetic Map.

Thus, the CIM analysis revealed that markers within the interval flankedby and including markers SEQ ID NO: 6 and SEQ ID NO: 17 were highlyassociated with FSR-8.01 (p-value≦0.01). Markers bordering FSR-8.01 alsofind utility with this invention, but their associations with thatinterval tend to decrease as their locations become further removed fromFSR-8.01.

Example 4 Detecting FSR Resistance in a Population of Plants andMonitoring the Introgression of FSR Resistance Loci from One Plant Lineinto Another Via MAS

A population of corn plants can be phenotyped using any method thatgauges the effect of FSR infection on a plant trait, including themethods described herein. The genotypes of the plants in the populationat one or more markers that map to the FSR-3.01 or FSR-8.01 chromosomeintervals, or at one or more markers closely linked to one of thoseintervals, can also be determined. In one embodiment, statisticalassociations can then be made between the recorded phenotypes and thegenotypes using a variety of methods known in the art, including thosedescribed herein.

In one embodiment, genotypes of offspring derived from one or moreindividuals in the population can be compared to the genotypes of theparents at one or more marker loci linked to the FSR-3.01 or FSR-8.01genotypes of the parents at those same loci. Individuals that sharemarker genotypes with the resistant parent at one or more markers canthen be selected for advancement in the breeding program. Individualsthat do not share marker genotypes with the resistant parent, orindividuals that do share marker genotypes with the susceptible parent,can be discarded. This process saves the laborious and time consumingprocess of phenotyping plants to verify which are resistant orsusceptible.

In some embodiments, useful markers comprise any marker that is withinor genetically linked to FSR-3.01 or FSR-8.01. In other embodiments, usemarkers comprise any marker that is between publically available markerspsk2 and gpm753d. In other embodiments, useful markers comprise anymarker that is between publically available markers umc1790 andmHbrBC384-Mo17. In other embodiments, associations are made betweengenotypes for one or more SNP markers that map between publicallyavailable markers psk2 and gpm753d or umc1790 and mHbrBC384-Mo17.

Selections and assays may be performed on single loci, or simultaneouslyon multiple loci. For example, a breeder skilled in the art could baseadvancement decisions on the genotypes of markers linked to FSR-3.01 orFSR-8.01 and genotypes of markers linked to other loci, simultaneously.For instance, a breeder may require that the same plant must exhibitgenotypes at one or more markers linked to FSR-3.01 or FSR-8.01 and/orat one or more markers linked to any other locus in order to beadvanced. In one embodiment, a breeder may require that the same plantmust exhibit genotypes at one or more markers linked to FSR-3.01 andFSR-8.01 in order to be advanced. In other embodiments, a singlegenotype at only one locus may be sufficient for advancement.

By selecting only those individuals with the desired genotype foradvancement in the breeding program, the frequency of desired allelesand desired phenotypes can be increased in future generations.

The introgression of one or more desired loci from a donor line intoanother is achieved via repeated backcrossing to a recurrent parentaccompanied by selection to retain one or more FSR resistance loci fromthe donor parent. Markers associated with FSR resistance are assayed inprogeny and those progeny with one or more FSR resistance markers areselected for advancement. In another aspect, one or more markers can beassayed in the progeny to select for plants with the genotype of theagronomically elite parent. This invention anticipates that traitintrogression activities will require more than one generation, whereinprogeny are crossed to the recurrent (agronomically elite) parent orselfed. Selections are made based on the presence of one or more FSRresistance markers and can also be made based on the recurrent parentgenotype, wherein screening is performed on a genetic marker and/orphenotype basis. In another embodiment, markers of this invention can beused in conjunction with other markers, ideally at least one on eachchromosome of the corn genome, to track the introgression of otherdesired traits as well as FSR resistance loci into elite germplasm. Inyet another embodiment, at least 100 SNP markers assorted across the 10chromosomes of corn will be useful in conjunction with the SNP molecularmarkers of the present invention to follow the introgression of otherdesired traits as well as FSR resistance into elite germplasm. In apreferred embodiment, about three hundred fifty SNP markers, distributedevery 5 centimorgans across the 10 chromosomes of the corn geneticlinkage map, will be useful in conjunction with the SNP molecularmarkers of the present invention to follow the introgression of otherdesired traits as well as FSR resistance into elite germplasm. Inanother embodiment, QTLs associated with FSR resistance will be usefulin conjunction with SNP molecular markers of the present invention tocombine quantitative and qualitative FSR resistance in the same plant.It is within the scope of this invention to utilize the methods andcompositions for trait integration of FSR resistance. It is contemplatedthat the present invention will be useful for developing commercialvarieties with FSR resistance and an agronomically elite phenotype.

For example, one skilled in the art can use one or more markers linkedto FSR-3.01 or FSR-8.01, for example, those listed in Table 1a or Table1b, to select plants for FSR resistance genotypes arising from the donorwhile selecting for the recipient genotypes in adjacent chromosomeregions. In practice, this reduces the amount of linkage drag from thedonor genome that maybe associated with undesirable agronomicproperties. This backcrossing procedure is implemented at any stage inline development and occurs in conjunction with breeding for superioragronomic characteristics or one or more traits of interest, includingtransgenic and nontransgenic traits.

Alternatively, a forward breeding approach is employed wherein one ormore FSR resistance loci can be monitored for successful introgressionfollowing a cross with a susceptible parent with subsequent generationsgenotyped for one or more FSR resistance loci and for one or moreadditional traits of interest, including transgenic and nontransgenictraits.

This invention can be used on populations other than those specificallydescribed in this application without altering the methods describedherein. Although different parents may have different genotypes atdifferent markers, the method of using this invention is fundamentallyidentical. Parents are first phenotyped for FSR resistance, genotyped ateach marker, and then those genotypes are used to infer resistant orsusceptible phenotypes in progeny derived from those parents or in anyother population where the genotypes are associated with the samephenotypes.

Example 5 Fine-Mapping of FSR-3.01 by Joint Linkage Analysis

The original QTL identification was from three bi-parental mappingpopulations. The three bi-parental populations were merged for jointlinkage mapping (Table 9). CV056629/CV344635, CV334995/1180580 andCV374246/CV344635. CV344635 is described in U.S. Pat. No. 8,471,127issued Jun. 25, 2013, which is incorporated herein by reference in itsentirety. CV334995 is described in U.S. Pat. No. 7,709,709 issued May 4,2010, which is incorporated herein by reference in its entirety. I180580is described in U.S. Pat. No. 7,173,171 issued Feb. 6, 2007, which isincorporated herein by reference in its entirety. CV374246 is describedin U.S. Pat. No. 7,875,775 issued Jan. 25, 2011, which is incorporatedherein by reference in its entirety. CV344635 was derived from I180580.

TABLE 9 Mapping Populations Project Population Population ID Mappingpopulation Resistant Line Susceptible Line Type Size A CV056629/CV344635CV344635 CV056629 F4 179 B CV334995/I180580 I180580 CV334995 F3 236 CCV374246/CV344635 CV344635 CV374246 DH 242 JOINT Multi-OriginI180580_CV344635 CV056629_CV374246_CV334995 JNT 644

To increase the marker density in the chromosome interval associatedwith FSR-3.01, 16 new SNP markers were developed (see Table 10) thatcollectively spanned 100.8-112.2 cM on chromosome 3 in the maize genome.The SNP position within the SEQ ID NO. is given in the second column.One of skill in the art will recognize that other sequences to eitherside of the given primers can also be effectively used, so long as theyprimers can amplify a region that includes the allele to be detected.Further, it will be appreciated that the precise probe to be used fordetection can vary, e.g., any probe that can identify the region of amarker amplicon to be detected can be substituted for those examplesprovided herein. Configuration of the amplification primers anddetection probes may also vary. Thus, the invention is not limited tothe primers, probes, or marker sequences specifically recited herein.

TABLE 10 Primers and probes useful for fine-mapping of FSR-3.01 SEQ IDNO. SEQ ID SNP Fwd Rev Probe NO. Position Primer Primer 1 Probe 2 86 216102 118 134 150 1 81 18 35 52 69 87 151 103 119 135 151 88 151 104 120136 152 89 194 105 121 137 153 90 101 106 122 138 154 91 151 107 123 139155 92 151 108 124 140 156 93 151 109 125 141 157 94 101 110 126 142 15895 151 111 127 143 159 96 180 112 128 144 160 97 151 113 129 145 161 98101 114 130 146 162 99 101 115 131 147 163 2 46 19 36 53 70 100 101 116132 148 164 101 200 117 133 149 165

644 plants were genotyped using the SNP markers shown in Table 10, andthe data was combined into previous genotyping information. Marker-traitassociation studies were performed using both least absolute shrinkageand selection operator (LASSO) (Robert Tibshirani, 1995) model andsingle-marker association (SMA) analysis. Table 11 provides the ProjectID, population type, number of markers used, chromosome position, leftand right flanking positions of this QTL on Monsanto's internalconsensus genetic map, additive effect, and phenotypic variance of thisQTL or Total (R²). The QTL associated with FSR-3.01 was fine-mapped to a5.9 cM interval (105-110.9 cM). The QTL effect for one copy of favorableallele was 0.542 rating score. FSR resistance ratings were generatedusing the methods described in Example 1. The phenotypic varianceexplained (R²) by this QTL was 21%.

TABLE 11 Summary of LASSO result in fine-mapping of FSR-3.01 ProjectPopulation ID Type #Mk Chr Left Right p-value Additive QTL R² Total R²Joint Inbred 329 3 105 110.9 0.01 0.542 0.213 0.413

Table 12 lists the estimated effects of markers associated with FSR-3.01in joint linkage mapping by single-marker association (SMA) analysis.Each row provides the SEQ ID NO of the marker, genetic map loci arerepresented in cM, with position zero being the first (most distal)marker known at the beginning of the chromosome on Monsanto's internalconsensus genetic map, F statistical value, p-value and the estimatedeffect that the marker polymorphism had on the FSR phenotype.

TABLE 12 Allele effects of markers associated with FSR-3.01 in jointlinkage mapping. Permutation testing Single Allele SEQ ID NO. MON Map cMFstat Probability Effect 86 100.8 182.3 0.001 0.56 1 102.2 180.9 0.0010.56 87 102.5 185.1 0.001 0.56 88 104.0 188.3 0.001 0.57 89 104.2 187.40.001 0.56 90 105.0 190.5 0.001 0.57 91 106.2 163.1 0.001 0.54 92 106.2163.7 0.001 0.54 93 107.1 174.7 0.001 0.56 94 108.5 174.0 0.001 0.56 95108.7 173.1 0.001 0.55 96 108.8 163.1 0.001 0.54 97 109.0 167.4 0.0010.55 98 109.3 164.6 0.001 0.55 99 110.3 173.0 0.001 0.56 2 110.9 181.80.001 0.57 100 111.8 173.1 0.001 0.55 101 112.2 161.9 0.001 0.53*P-value is based on 10,000 permutation tests.

For example, SEQ ID NO: 86 was associated with a 0.56 change in FSRresistance rating by one copy of the favorable allele.

Example 6 Annotated Genes within FSR-3.01 or FSR-8.01

Table 13 lists annotated coding sequences within the FSR-3.01 andFSR-8.01 regions. Each row provides gene ID, gene annotation, chromosomelocation, genetic position on Monsanto internal consensus map andphysical position based on Arizona Genomics Institute B73 RefGen_v2sequence which is publicly available. Transgenic maize resistant toFusarium stalk rot can be generated using these annotated genes asdescribed herein.

TABLE 13 Annotated coding sequences within the FSR-3.01 and FSR-8.01regions. MON Physical Map Position Gene Map bp†† ID Annotation Chr cM†Start End 1 Histidine kinase 2 n = 1 Tax = Zea mays RepID = Q76H00_MAIZE(0.0); CHASE: CHASE domain (3.9e−68); HisKA: His Kinase A(phosphoacceptor) 3 102.2 158889193 158896487 domain (1.2e−24);HATPase_c: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase(2.9e−31); Response_reg: Response regulator receiver domain (4.5e−25);GO_MF:GO:0016772, transferase activity, transferringphosphorus-containing groups# (0.0); GO_BP:GO:0018106,peptidyl-histidine phosphorylation# (0.0); GO_CC:GO:0016020, membrane#(0.0) 2 OSJNBa0053B21.7 protein n = 3 Tax = Oryza sativa RepID =Q7XKR4_ORYSJ (1e−145); CG-1: CG-1 domain (3.6e−78); Ank: Ankyrin repeat(13); Ank: 3 102.2 158894821 158901970 Ankyrin repeat (5e−06);Topo-VIb_trans: Topoisomerase VI B subunit, transducer (0.08); IQ: IQcalmodulin-binding motif (18); IQ: IQ calmodulin-binding motif (0.004);IQ: IQ calmodulin-binding motif (0.0036); GO_MF:GO:0030528,transcription regulator activity# (0.0); GO_BP:GO:0045449, regulation oftranscription# (0.0); GO_CC:GO:0005634, nucleus# (0.0) 3 HAT familydimerisation domain containing protein n = 4 Tax = Oryza sativa JaponicaGroup RepID = Q7G4F1_ORYSJ (1e−176); hATC: hAT 3 102.2 158915841158918155 family dimerisation domain (2.9e−32); GO_MF:GO:0046983,protein dimerization activity# (1e−176); GO_BP:GO:0032196,transposition# (1e−136) 4 CBS domain containing protein n = 5 Tax =Poaceae RepID = B6U1W0_MAIZE (0.0); CBS: CBS domain (5.7e−25); CBS: CBSdomain (3e−20); PB1: 3 102.2 158974015 158978428 PB1 domain (6.3e−16);GO_MF:GO:0003824, catalytic activity# (3e−55); GO_BP:GO:0008152,metabolic process# (3e−55) 5 MADS-box transcription factor 8 n = 3 Tax =Zea mays RepID = B6T9L2_MAIZE (3e−64); SRF-TF: SRF-type transcriptionfactor (DNA-binding and 3 102.2 158979849 159007027 dimerisation domain)(1.8e−25); GO_MF:GO:0043565, sequence-specific DNA binding# (3e−64);GO_BP:GO:0045449, regulation of transcription# (3e−64);GO_CC:GO:0005634, nucleus# (3e−64) 6 Transposase n = 1 Tax = Zea maysRepID = A5X2G8_MAIZE (1e−176); hATC: hAT family dimerisation domain(7.1e−29); GO_MF:GO:0046983, 3 102.2 159003437 159006179 proteindimerization activity# (0.0); GO_BP:GO:0006468, protein amino acidphosphorylation# (1e−43) 7 Putative uncharacterized protein n = 1 Tax =Zea mays RepID = B6T0P1_MAIZE (9e−21) 3 102.2 159169715 159170268 8Squamosa promoter-binding-like protein 2 n = 2 Tax = Oryza sativa RepID= SPL2_ORYSJ (6e−67); SBP: SBP domain (1.9e−48); 3 102.3 159377754159381056 GO_MF:GO:0046872, metal ion binding# (6e−67);GO_BP:GO:0045449, regulation of transcription# (6e−67);GO_CC:GO:0005634, nucleus# (6e−67) 9 C/VIF2 n = 2 Tax = Zea mays RepID =B6TMN1_MAIZE (9e−16); PMEI: Plant invertase/pectin methylesteraseinhibitor (1.5e−19); 3 102.3 159479564 159480232 GO_MF:GO:0030599,pectinesterase activity# (4e−28); GO_BP:GO:0004857, enzyme inhibitoractivity# (4e−28) 10 Putative basic helix-loop-helix protein BHLH12 n =1 Tax = Lotus japonicus RepID = C0JP17_LOTJA (6e−32); HLH:Helix-loop-helix DNA-binding domain 3 102.3 159485166 159485874(0.0003); GO_MF:GO:0030528, transcription regulator activity# (3e−39);GO_BP:GO:0045449, regulation of transcription# (3e−39);GO_CC:GO:0005634, nucleus# (3e−39) 11 Nucleobase ascorbate transporter n= 2 Tax = Populus trichocarpa RepID = B9MYJ5_POPTR (2e−39);Xan_ur_permease: 3 102.3 159604854 159607076 Permease family (0.069);GO_MF:GO:0005215, transporter activity# (3e−52); GO_BP:GO:0055085,transmembrane transport# (3e−52); GO_CC:GO:0016020, membrane# (3e−52) 1240S ribosomal protein S7 n = 2 Tax = Poaceae RepID = RS7_SECCE (3e−98);Ribosomal_S7e: Ribosomal protein S7e (3.3e−84); 3 102.3 159608077159610141 GO_MF:GO:0003735, structural constituent of ribosome# (3e−98);GO_BP:GO:0006412, translation# (3e−98); GO_CC:GO:0030529,ribonucleoprotein complex# (3e−98) 13 Helicase-like protein n = 1 Tax =Oryza sativa Japonica Group RepID = Q5VR06_ORYSJ (1e−114);GO_MF:GO:0004386, helicase activity# (1e−114) 3 102.3 159666647159667759 14 Putative retrotransposon protein n = 1 Tax = Phyllostachysedulis RepID = D3IVP0_9POAL (3e−86); DUF889: Eukaryotic protein 3 102.3159669204 159670250 of unknown function (DUF889) (1.2e−54);GO_MF:GO:0004386, helicase activity# (7e−83) 15 Retrotransposon proteinn = 1 Tax = Zea mays RepID = B6U894_MAIZE (8e−44); GO_MF:GO:0004386,helicase activity# (1e−28) 3 102.3 159670783 159673146 16 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6UBS8_MAIZE(4e−91) 3 102.3 159677432 159679346 17 HAT dimerisationdomain-containing protein, putative n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q84MT5_ORYSJ (3e−32); 3 102.3 159685435 159690179GO_MF:GO:0046983, protein dimerization activity# (3e−30) 18 Putativereverse transcriptase n = 1 Tax = Sorghum bicolor RepID = Q8LJX1_SORBI(2e−49); GO_MF:GO:0003964, RNA-directed DNA polymerase, 3 102.5159704324 159705004 group II intron encoded# (2e−49); GO_BP:GO:0006278,RNA-dependent DNA replication# (2e−49) 19 Acyl-[acyl-carrier-protein]desaturase, chloroplastic n = 1 Tax = Elaeis guineensis RepID =STAD_ELAGV (1e−175); FA_desaturase_2: 3 103.3 160666708 160671413 Fattyacid desaturase (4.5e−244); GO_MF:GO:0046914, transition metal ionbinding# (0.0); GO_BP:GO:0055114, oxidation reduction# (0.0);GO_CC:GO:0009536, plastid# (1e−175) 20 Hydrolase-like protein n = 1 Tax= Zea mays RepID = B6SRX1_MAIZE (1e−163); Abhydrolase_1: alpha/betahydrolase fold (4.4e−09); 3 103.3 160750607 160754718 GO_MF:GO:0016787,hydrolase activity# (1e−163) 21 Probable auxin efflux carrier component6 n = 4 Tax = Poaceae RepID = PIN6_ORYSJ (1e−159); Mem_trans: Membranetransport protein 3 103.35 160753025 160757157 (1.1e−72);GO_MF:GO:0010329, IDA#auxin efflux transmembrane transporter activity#(3e−43); GO_BP:GO:0055085, transmembrane transport# (1e−159);GO_CC:GO:0016021, integral to membrane# (1e−159) 22 OJ1485_B09.11protein n = 3 Tax = Oryza sativa RepID = Q8RZI5_ORYSJ (7e−87); F-box:F-box domain (0.01); Sell: Sell repeat (5.9); Sell: 3 103.45 159817059159819103 Sell repeat (0.0093); Sell: Sell repeat (0.079); zf-MYND: MYNDfinger (8.7e−09); GO_MF:GO:0008270, zinc ion binding# (7e−87);GO_CC:GO:0005634, nucleus# (9e−55) 23 Putative transformerserine/arginine-rich ribonucleoprotein n = 2 Tax = Oryza sativa RepID =Q84QA6_ORYSJ (1e−26); RRM_1: RNA 3 103.7 160563885 160566370 recognitionmotif. (a.k.a. RRM, RB (7.9e−10); GO_MF:GO:0003676, nucleic acidbinding# (5e−29); GO_BP:GO:0008380, RNA splicing# (1e−17);GO_CC:GO:0030529, ribonucleoprotein complex# (1e−17) 24 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6UD01_MAIZE(6e−12) 3 103.75 160559981 160560684 25 Hydrolase-like protein n = 1 Tax= Zea mays RepID = B6SRX1_MAIZE (3e−40); GO_MF:GO:0016787, hydrolaseactivity# (3e−40) 3 103.8 160785443 160785875 26 FK506-binding protein2-1 n = 3 Tax = Andropogoneae RepID = B4FUK2_MAIZE (3e−69); FKBP_C:FKBP-type peptidyl-prolyl cis-trans isomeras 3 103.8 161953049 161955764(3.5e−55); GO_MF:GO:0016853, isomerase activity# (3e−69);GO_BP:GO:0006457, protein folding# (3e−69); GO_CC:GO:0005788,endoplasmic reticulum lumen# (8e−54) 27 Transcription elongation factor1 homolog n = 7 Tax = Poaceae RepID = ELOF1_ORYSJ (4e−35); Elf1:Transcription elongation factor Elf1 like 3 103.9 160787852 160788759(9.3e−48); GO_MF:GO:0046872, metal ion binding# (4e−35);GO_BP:GO:0045449, regulation of transcription# (4e−35);GO_CC:GO:0005634, nucleus# (4e−35) 28 Loricrin n = 2 Tax = Zea maysRepID = B6SSB2_MAIZE (9e−50) 3 104 161893091 161894313 29 AP-1 complexsubunit gamma-2, putative n = 1 Tax = Ricinus communis RepID =B9S1S1_RICCO (0.0); Adaptin_N: Adaptin N terminal 3 104.1 161847000161856031 region (2.7e−57); HEAT: HEAT repeat (9.8e−05); HEAT: HEATrepeat (12); GO_MF:GO:0005515, protein binding# (0.0); GO_BP:GO:0016192,vesicle-mediated transport# (0.0); GO_CC:GO:0030117, membrane coat#(0.0) 30 E2 protein isoform 5 n = 2 Tax = Zea mays RepID = B6TAW6_MAIZE(2e−48); EnY2: Transcription factor e(y)2 (1.8e−36); GO_MF:GO:0030374, 3104.3 160512467 160515039 IDA#ligand-dependent nuclear receptortranscription coactivator activity# (4e−17); GO_BP:GO:0051028, mRNAtransport# (4e−17); GO_CC:GO:0009941, IDA#chloroplast envelope# (2e−24)31 Putative uncharacterized protein Sb03g043990 n = 2 Tax =Andropogoneae RepID = C5XGA1_SORBI (6e−80); GO_MF:GO:0005515, 3 104.3160515583 160518688 protein binding# (6e−10); GO_CC:GO:0005886, plasmamembrane# (6e−10) 32 Dynamin, putative n = 1 Tax = Ricinus communisRepID = B9T3E4_RICCO (0.0); MMR_HSR1: GTPase of unknown function(0.0016); 3 104.4 160397321 160408459 Dynamin_N: Dynamin family(1.6e−89); Dynamin_M: Dynamin central region (3.7e−134); GED: DynaminGTPase effector domain (6e−36); GO_MF:GO:0005525, GTP binding# (0.0);GO_BP:GO:0051301, cell division# (0.0); GO_CC:GO:0016020, membrane#(0.0) 33 Catalytic/protein phosphatase type 2C n = 2 Tax = Zea maysRepID = B6TWB0_MAIZE (1e−15); GO_MF:GO:0003824, catalytic activity#(1e−15); 3 104.4 160430829 160431484 GO_BP:GO:0004721, phosphoproteinphosphatase activity# (2e−11) 34 Catalytic/protein phosphatase type 2C n= 2 Tax = Zea mays RepID = B6TWB0_MAIZE (1e−12); GO_MF:GO:0003824,catalytic activity# (1e−12) 3 104.4 160431696 160432351 35 Putativeuncharacterized protein 9C20.7 n = 1 Tax = Zea mays RepID = Q5NKP3_MAIZE(4e−09) 3 104.4 160432563 160433166 36 Catalytic/protein phosphatasetype 2C n = 2 Tax = Zea mays RepID = B6TWB0_MAIZE (2e−14);GO_MF:GO:0003824, catalytic activity# (2e−14); 3 104.4 160433431160434086 GO_BP:GO:0004721, phosphoprotein phosphatase activity# (3e−11)37 Hypersensitivity-induced response-like protein n = 1 Tax = Cenchrusciliaris RepID = Q9ATP0_CENCI (8e−20); GO_MF:GO:0005515, protein 3 104.4161765290 161765490 binding# (4e−18); GO_CC:GO:0016020, membrane#(2e−18) 38 OJ1485_B09.2 protein n = 2 Tax = Oryza sativa RepID =Q8RUF2_ORYSJ (8e−92); Nucleotid_trans: Nucleotide-diphospho-sugartransferas 3 104.5 160303613 160305557 (0.036); GO_MF:GO:0003690,IDA#double-stranded DNA binding# (3e−90); GO_BP:GO:0006265, DNAtopological change# (3e−90); GO_CC:GO:0005634, nucleus# (9e−58) 39OJ1485_B09.3 protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q8RZJ3_ORYSJ (1e−82); GO_MF:GO:0003677, DNA 3 104.5 160327891 160329710binding# (4e−64); GO_BP:GO:0045449, regulation of transcription#(4e−64); GO_CC:GO:0005634, nucleus# (4e−64) 40 Putative transposase n =1 Tax = Zea mays RepID = Q8W0Y1_MAIZE (4e−25) 3 104.5 160331611160332429 41 Putative uncharacterized protein n = 1 Tax = Zea mays RepID= B6SP05_MAIZE (3e−14) 3 104.6 161695642 161697306 42 Putativeuncharacterized protein Sb03g043790 n = 1 Tax = Sorghum bicolor RepID =C5XG81_SORBI (0.0); GO_MF:GO:0003824, catalytic 3 104.6 161700194161703481 activity# (1e−115) 43 Mannosyl-oligosaccharide glucosidase,putative n = 1 Tax = Ricinus communis RepID = B9RMG4_RICCO (0.0);Glyco_hydro_63: Mannosyl 3 104.7 160074262 160087237 oligosaccharideglucosidase (1.9e−60); GO_MF:GO:0004573, mannosyl-oligosaccharideglucosidase activity# (0.0); GO_BP:GO:0009311, IDA#oligosaccharidemetabolic process# (0.0); GO_CC:GO:0005783, IDA#endoplasmic reticulum#(1e−177) 44 Putative retrotransposon protein n = 1 Tax = Phyllostachysedulis RepID = D3IVT4_9POAL (1e−19); GO_MF:GO:0008270, 3 104.7 160113302160113547 zinc ion binding# (6e−19); GO_BP:GO:0015074, DNA integration#(6e−19) 45 Putative uncharacterized protein Sb04g006710 n = 1 Tax =Sorghum bicolor RepID = C5XXI6_SORBI (1e−124); GO_MF:GO:0003964,RNA-directed 3 104.7 160141479 160143446 DNA polymerase, group II intronencoded# (6e−11); GO_BP:GO:0007165, signal transduction# (5e−12);GO_CC:GO:0005886, plasma membrane# (1e−12) 46 OSJNBa0083I11.5 protein n= 1 Tax = Oryza sativa Japonica Group RepID = Q7XM33_ORYSJ (9e−57);RVT_2: Reverse transcriptase 3 104.7 160167145 160167771 (RNA-dependentDNA pol (2.4e−06); GO_MF:GO:0008270, zinc ion binding# (5e−58);GO_BP:GO:0015074, DNA integration# (5e−58); GO_CC:GO:0016021, integralto membrane# (2e−53) 47 Protein kinase APK1B, chloroplast, putative n =1 Tax = Ricinus communis RepID = B9S1V9_RICCO (3e−10); GO_MF:GO:0005524,ATP binding# (4e−10); 3 104.7 161650237 161650694 GO_BP:GO:0006468,protein amino acid phosphorylation# (4e−10) 48 Zinc finger CCCH typedomain-containing protein ZFN-like 2 n = 3 Tax = Zea mays RepID =B6TK84_MAIZE (1e−166); zf-CCCH: 3 104.75 161643342 161649531 Zinc fingerC-x8-C-x5-C-x3 H type (and similar) (1.8e−09); zf-CCCH: Zinc fingerC-x8-C-x5-C-x3-H type (and similar) (3.6e−08); zf-CCCH: Zinc fingerC-x8-C-x5-C-x3-H type (and similar) (8.5e−11); zf-CCCH: Zinc fingerC-x8-C-x5-C-x3-H type (and similar) (3.3e−11); GO_MF:GO:0046872, metalion binding# (1e−166); GO_BP:GO:0009416, IEP#response to light stimulus#(5e−58); GO_CC:GO:0005634, nucleus# (1e−155) 49 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = B6SUB9_MAIZE (1e−69) 3 104.8159990193 160008489 50 Exostosin-like n = 2 Tax = Andropogoneae RepID =B6UFM1_MAIZE (0.0); Exostosin: Exostosin family (7.5e−96);GO_MF:GO:0016740, 3 104.8 160013230 160017242 transferase activity#(1e−75); GO_BP:GO:0048868, IMP#pollen tube development# (5e−65);GO_CC:GO:0016020, membrane# (0.0) 51 Transposon protein CACTA, En/Spmsub-class n = 1 Tax = Zea mays RepID = B6U6W8_MAIZE (2e−43);GO_MF:GO:0046872, metal ion binding# (9e−09); 3 104.8 160020221160021299 GO_BP:GO:0016070, TAS#RNA metabolic process# (9e−09) 52Putative DNA repair protein rhp54 n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q7XI63_ORYSJ (3e−34); GO_MF:GO:0005524, ATP binding#(7e−35); 3 104.8 161456374 161456877 GO_BP:GO:0045449, regulation oftranscription# (3e−20); GO_CC:GO:0005634, nucleus# (3e−20) 53 Putativeauxin-independent growth promoter n = 2 Tax = Oryza sativa RepID =Q6Z341_ORYSJ (7e−25) 3 104.8 161464517 161466445 54 ER lumen proteinretaining receptor n = 1 Tax = Oryza sativa Japonica Group RepID =B9FES2_ORYSJ (4e−19); GO_MF:GO:0005515, protein binding# (2e−27); 3104.8 161558818 161562477 GO_BP:GO:0010165, response to X-ray# (2e−27);GO_CC:GO:0005634, nucleus# (2e−27) 55 Putative leucine zipper protein n= 1 Tax = Oryza sativa Japonica Group RepID = Q8RZJ0_ORYSJ (0.0); Exo70:Exo70 exocyst complex subunit (5.5e−164); 3 104.85 159939500 159942907GO_BP:GO:0006887, exocytosis# (0.0); GO_CC:GO:0000145, NAS#exocyst#(0.0) 56 Mitochondrial import receptor subunit TOM20 n = 2 Tax =Andropogoneae RepID = B6SZD1_MAIZE (1e−101); TOM20_plant: Plant specific3 104.9 159873679 159879241 mitochondrial import recep (9.6e−128);GO_MF:GO:0005515, protein binding# (1e−101); GO_BP:GO:0045040, proteinimport into mitochondrial outer membrane# (1e−101); GO_CC:GO:0005742,mitochondrial outer membrane translocase complex# (1e−101) 57OJ1485_B09.7 protein n = 3 Tax = Oryza sativa RepID = Q8RZI9_ORYSJ(2e−48); GO_MF:GO:0016301, kinase activity# (4e−29); GO_BP:GO:006301, 3104.9 159936961 159938372 kinase activity# (4e−29); GO_CC:GO:0009505,IDA#expansin# (6e−29) 58 Putative uncharacterized protein n = 1 Tax =Zea mays RepID = C4JC41_MAIZE (1e−112); FYVE: FYVE zinc finger (0.083);zf-MIZ: MIZ zinc finger (0.079); 3 104.95 160932864 160936118 zf-C3HC4:Zinc finger, C3HC4 type (RING finger) (6e−09); GO_MF:GO:0046872, metalion binding# (1e−112) 59 Sucrose-phosphate synthase n = 3 Tax =Andropogoneae RepID = SPS_MAIZE (0.0); Glycos_transf_1: Glycosyltransferases group 1 3 104.95 161252328 161258511 (8.1e−22); S6PP:Sucrose-6F-phosphate phosphohydrolase (1.1e−05); GO_MF:GO:0046524,sucrose-phosphate synthase activity# (0.0); GO_BP:GO:0009058,biosynthetic process# (0.0); GO_CC:GO:0005886, plasma membrane# (0.0) 60Putative extra sporogenous cells n = 2 Tax = Oryza sativa RepID =Q7F8Q9_ORYSJ (0.0); LRRNT_2: Leucine rich repeat N-terminal domain 3104.95 161580031 161583912 (3.8e−06); LRR_1: Leucine Rich Repeat (0.94);LRR_1: Leucine Rich Repeat (0.002); LRR_1: Leucine Rich Repeat (4.3);LRR_1: Leucine Rich Repeat (2.3); LRR_1: Leucine Rich Repeat (0.99);LRR_1: Leucine Rich Repeat (8.3); LRR_1: Leucine Rich Repeat (1.7);LRR_1: Leucine Rich Repeat (6.3); LRR_1: Leucine Rich Repeat (12);LRR_1: Leucine Rich Repeat (0.75); LRR_1: Leucine Rich Repeat (5.6);LRR_1: Leucine Rich Repeat (0.64); LRR_1: Leucine Rich Repeat (3.7);LRR_1: Leucine Rich Repeat (2.6); LRR_1: Leucine Rich Repeat (0.91);LRR_1: Leucine Rich Repeat (0.11); LRR_1: Leucine Rich Repeat (54);LRR_1: Leucine Rich Repeat (1.7); LRR_1: Leucine Rich Repeat (1.2);LRR_1: Leucine Rich Repeat (0.34); LRR_1: Leucine Rich Repeat (0.02);LRR_1: Leucine Rich Repeat (8.5); LRR_1: Leucine Rich Repeat (0.78);LRR_1: Leucine Rich Repeat (11); LRR_1: Leucine Rich Repeat (1.8);LRR_1: Leucine Rich Repeat (0.069); LRR_1: Leucine Rich Repeat (14);Pkinase: Protein kinase domain (7.5e−36); Pkinase_Tyr: Protein tyrosinekinase (1.9e−24); GO_MF:GO:0016301, kinase activity# (0.0);GO_BP:GO:0048658, PMID:17727613#tapetal layer development# (0.0);GO_CC:GO:0016021, integral to membrane# (0.0) 61 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = COPM38_MAIZE(3e−10); GO_MF:GO:0017111, nucleoside-triphosphatase activity# (3e−10);3 105 160872388 160877158 GO_BP:GO:0009432, SOS response# (3e−10);GO_CC:GO:0005737, cytoplasm# (3e−10) 62 Lysine ketoglutarate reductasetrans-splicing related 1-like n = 3 Tax = Oryza sativa RepID =Q5JLN0_ORYSJ (1e−148); DUF707: 3 105 160899188 160902589 Protein ofunknown function (DUF707) (2.8e−205) 63 Putative uncharacterized proteinn = 1 Tax = Zea mays RepID = B6T227_MAIZE (1e−09) 3 105 161259756161260190 64 Zgc:162613 protein n = 3 Tax = Danio rerio RepID =A3KNW8_DANRE (1e−39); DUF647: Protein of unknown function, DUF647(1.5e−177); 3 105 161363754 161369525 GO_MF:GO:0016740, transferaseactivity# (5e−38); GO_BP:GO:0032502, IMP#developmental process#(1e−166); GO_CC:GO:0005576, extracellular region# (0.0) 65 Zincfinger-like protein (Fragment) n = 1 Tax = Phaseolus vulgaris RepID =Q84U29_PHAVU (8e−15); zf-C3HC4: Zinc finger, C3HC4 type (RING finger) 3105 161565312 161566687 (9.1e−05); GO_MF:GO:0046872, metal ion binding#(1e−102) 66 Wiscott-Aldrich syndrome, C-terminal n = 1 Tax = Zea maysRepID = B6U4M1_MAIZE (7e−48); B12D: B12D protein (2.8e−06); 3 105161567574 161574608 PBD: P21-Rho-binding domain (3.2e−09) 67 TA1 protein(Fragment) n = 1 Tax = Oryza sativa Japonica Group RepID = Q70KS8_ORYSJ(1e−85); HLH: Helix-loop-helix DNA-binding domain (1.1e−07); 3 107.15162063107 162066385 GO_MF:GO:0030528, transcription regulator activity#(0.0); GO_BP:GO:0045449, regulation of transcription# (0.0);GO_CC:GO:0005634, nucleus# (0.0) 68 Os01g0178200 protein n = 2 Tax =Oryza sativa RepID = Q5VRD8_ORYSJ (1e−22); DUF92: Integral membraneprotein DUF92 (4.7e−05) 3 107.6 162086845 162091358 69 Putativeuncharacterized protein Sb03g043660 n = 1 Tax = Sorghum bicolor RepID =C5XG65_SORBI (8e−63); DUF506: Protein of unknown 3 107.6 162095278162096162 function (DUF506) (3.8e−50) 70 Minor histocompatibilityantigen H13, putative n = 1 Tax = Ricinus communis RepID = B9SUL7_RICCO(1e−88); PA: PA domain (1.4e−19); 3 107.6 162175457 162180323Peptidase_A22B: Signal peptide peptidase (2.3e−26); GO_MF:GO:0004190,penicillopepsin activity# (1e−160); GO_BP:GO:0050819, negativeregulation of coagulation# (8e−23); GO_CC:GO:0016021, integral tomembrane# (1e−160) 71 SH3 domain-containing protein n = 1 Tax =Trifolium repens RepID = D3YBF4_TRIRP (1e−16); GO_MF:GO:0005515, proteinbinding# (2e−14); 3 107.6 162280029 162280752 GO_CC:GO:0005737,cytoplasm# (2e−14) 72 Pentatricopeptide repeat-containing protein,putative n = 1 Tax = Ricinus communis RepID = B9T3T5_RICCO (1e−112);PPR: PPR repeat (3.4); PPR: 3 107.6 162296687 162298725 PPR repeat(0.00045); PPR: PPR repeat (3.8e−11); PPR: PPR repeat (3.3e−06); PPR:PPR repeat (1.9); PPR: PPR repeat (2.5); PPR: PPR repeat (0.59);GO_MF:GO:0005488, binding# (1e−109); GO_BP:GO:0016556, IMP#mRNAmodification# (2e−96); GO_CC:GO:0009536, plastid# (2e−96) 73 Putativeuncharacterized protein Sb03g043620 n = 1 Tax = Sorghum bicolor RepID =C5XG62_SORBI (3e−50); GO_BP:GO:0010375, 3 107.6 162312872 162314047IMP#stomatal complex patterning# (3e−18) 74 Protein MYG1, putative n = 1Tax = Ricinus communis RepID = B9RYS8_RICCO (2e−45); UPF0160:Uncharacterised protein family (UPF0160) (4.9e−11); 3 107.6 162317907162323226 Tryp_alpha_amyl: Protease inhibitor/seed storage/LTP f(0.059); GO_MF:GO:0016151, nickel ion binding# (8e−20);GO_BP:GO:0043473, IMP#pigmentation# (1e−19); GO_CC:GO:0005739,mitochondrion# (2e−48) 75 Protease inhibitor/seed storage/LTP familyprotein n = 4 Tax = Zea mays RepID = B6ST99_MAIZE (2e−14);Metallothio_Pro: Prokaryotic metallothionein (0.05); 3 107.6 162372177162372497 Tryp_alpha_amyl: Protease inhibitor/seed storage/LTP f (0.07);GO_MF:GO:0008233, peptidase activity# (2e−14) 76 OSJNBa0086O06.17protein n = 2 Tax = Oryza sativa Japonica Group RepID = Q7XLZ2_ORYSJ(2e−99); GO_MF:GO:0004803, transposase activity# (4e−48); 3 107.6162388373 162390026 GO_BP:GO:0006313, transposition, DNA-mediated#(4e−48) 77 Xyloglucan endotransglucosylase/hydrolase protein 32 n = 2Tax = Zea mays RepID = B6T9H5_MAIZE (1e−22); GO_MF:GO:0016787, hydrolaseactivity# (1e−22); 3 107.6 162390710 162391087 GO_BP:GO:0006073,cellular glucan metabolic process# (1e−22); GO_CC:GO:0048046,IDA#apoplast# (1e−22) 78 Putative uncharacterized protein Sb03g043580 n= 1 Tax = Sorghum bicolor RepID = C5XG58_SORBI (4e−11) 3 107.7 162527626162527856 79 cDNA clone: J013092F14, full insert sequence n = 2 Tax =Poaceae RepID = B7ECT5_ORYSJ (3e−24); GO_CC:GO:0005886, plasma membrane#(4e−14) 3 107.7 162559310 162559546 80 Putative uncharacterized proteinn = 1 Tax = Zea mays RepID = B6TFE5_MAIZE (2e−65); DUF538: Protein ofunknown function, DUF538 (3.1e−47); GO_MF: 3 107.7 162599043 162600050GO:0043565, sequence-specific DNA binding# (5e−20); GO_BP:GO:0045449,regulation of transcription# (5e−20); GO_CC:GO:0005773, IDA#vacuole#(2e−29) 81 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =C4IYL7_MAIZE (9e−62) 3 107.7 162600661 162601008 82 Pentatricopeptiderepeat-containing protein, putative n = 1 Tax = Ricinus communis RepID =B9RTF6_RICCO (2e−82); TPR_4: Tetratricopeptide repeat (3.8); 3 107.7162602021 162603645 PPR: PPR repeat (0.0044); TPR_4: Tetratricopeptiderepeat (6.6); PPR: PPR repeat (2.1e−07); TPR_4: Tetratricopeptide repeat(21); PPR: PPR repeat (8.3e−06); PPR: PPR repeat (0.91); PPR: PPR repeat(0.49); GO_MF:GO:0005488, binding# (9e−85); GO_BP:GO:0032259,methylation# (7e−78); GO_CC:GO:0005739, mitochondrion# (3e−77) 83Uncharacterized ACR, COG1565 family protein n = 2 Tax = Zea mays RepID =B6TEQ7_MAIZE (0.0); DUF566: Family of unknown function (DUF566)(4.7e−06); 3 107.7 162692864 162701741 Malic_M: Malic enzyme, NADbinding domain (1.2e−06); DUF185: Uncharacterized ACR, COG1565(1.9e−49); GO_MF:GO:0051287, NAD or NADH binding# (1e−35);GO_BP:GO:0055114, oxidation reduction# (1e−35); GO_CC:GO:0005622,intracellular# (2e−34) 84 Heavy meromyosin-like n = 2 Tax = Oryza sativaRepID = Q8S0A4_ORYSJ (0.0) 3 107.7 162701976 162709719 85 Mitochondrialimport inner membrane translocase subunit TIM16 n = 4 Tax =Andropogoneae RepID = B6TGT3_MAIZE (1e−60); Pam16: Pam16 (1.4e−13); 3107.7 162741183 162743797 GO_MF:GO:0005215, transporter activity#(4e−52); GO_BP:GO:0006857, oligopeptide transport# (4e−52);GO_CC:GO:0016020, membrane# (4e−52) 86 Regulatory protein viviparous-1 n= 2 Tax = Zea mays RepID = VIV1_MAIZE (0.0); B3: B3 DNA binding domain(2.6e−21); GO_MF:GO:0003677, 3 107.7 162800024 162804983 DNA binding#(0.0); GO_BP:GO:0045449, regulation of transcription# (0.0);GO_CC:GO:0005737, cytoplasm# (0.0) 87 Abc transporter, putative n = 1Tax = Ricinus communis RepID = B9SPK8_RICCO (0.0); ABC_membrane: ABCtransporter transmembrane region 3 107.7 162813868 162818612 (4.7e−29);SMC_N: RecF/RecN/SMC N terminal domain (0.03); ABC_tran: ABC transporter(1.5e−57); GO_MF:GO:0042626, ATPase activity, coupled to transmembranemovement of substances# (0.0); GO_BP:GO:0055085, transmembranetransport# (0.0); GO_CC:GO:0016021, integral to membrane# (0.0) 88Ribophorin II, putative n = 1 Tax = Ricinus communis RepID =B9SV32_RICCO (0.0); Ribophorin_II: Ribophorin II (RPN2) (6.7e−201);GO_MF:GO:0004579, 3 107.7 162825595 162831853dolichyl-diphosphooligosaccharide-protein glycotransferase activity#(0.0); GO_BP:GO:0018279, protein amino acid N-linked glycosylation viaasparagine# (0.0); GO_CC:GO:0008250, oligosaccharyltransferase complex#(0.0) 89 Calcium binding atopy-related autoantigen 1 n = 2 Tax =Andropogoneae RepID = B6SL16_MAIZE (6e−35); efhand: EF hand (0.0033);GO_MF:GO:0005509, 3 107.8 163070674 163078478 calcium ion storageactivity# (6e−35) 90 F-box domain containing protein n = 1 Tax = Zeamays RepID = B6U9Q3_MAIZE (0.0); F-box: F-box domain (0.001); FBD: FBD(0.0025) 3 107.8 163085351 163088065 91 Putative uncharacterized proteinn = 2 Tax = Zea mays RepID = B6SQJ7_MAIZE (6e−36) 3 107.8 163330774163332783 92 Putative uncharacterized protein n = 2 Tax = Zea mays RepID= B6SQJ7_MAIZE (6e−36) 3 107.8 163443609 163445618 93 Putative gag-polpolyprotein n = 1 Tax = Zea mays RepID = Q8SA91_MAIZE (4e−25);GO_MF:GO:0004190, penicillopepsin activity# (4e−25); 3 107.9 163652562163657461 integration# (4e−25); GO_CC:GO:0005634, nucleus# (4e−25) 94Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6T8G3_MAIZE (2e−18) 3 107.9 163658731 163660184 95 DEAD-boxATP-dependent RNA helicase 30 n = 2 Tax = Oryza sativa Japonica GroupRepID = RH30_ORYSJ (0.0); GRP: Glycine rich 3 107.9 163743628 163748000protein family (0.0024); ResIII: Type III restriction enzyme, ressubunit (0.048); DEAD: DEAD/DEAH box helicase (1.2e−70); Helicase_C:Helicase conserved C-terminal domain (3.4e−34); GO_MF:GO:0016787,hydrolase activity# (0.0); GO_BP:GO:0042254, ribosome biogenesis# (0.0);GO_CC:GO:0005634, nucleus# (0.0) 96 Putative uncharacterized protein n =2 Tax = Zea mays RepID = B4FJK2_MAIZE (2e−44); GO_MF:GO:0003677, DNAbinding# (2e−44); 3 107.9 163748547 163749188 GO_BP:GO:0045449,regulation of transcription# (2e−44); GO_CC:GO:0005634, nucleus# (2e−44)97 SCAR-like protein 2 n = 3 Tax = Oryza sativa Japonica Group RepID =SCRL2_ORYSJ (2e−24); GO_MF:GO:0003779, actin binding# (2e−24); 3 107.9163751314 163751773 GO_CC:GO:0005856, cytoskeleton# (2e−24) 98 Putativeuncharacterized protein Sb05g000810 n = 1 Tax = Sorghum bicolor RepID =C5Y329_SORBI (4e−20); GO_MF:GO:0003725, 3 107.9 163754714 163755373IDA#double-stranded RNA binding# (1e−15); GO_CC:GO:0005622,intracellular# (1e−15) 99 EH-domain-containing protein 1 n = 3 Tax =Andropogoneae RepID = B6U193_MAIZE (1e−52); GO_MF:GO:0005525, GTPbinding# (1e−52); 3 107.9 163862316 163863436 GO_BP:GO:0004872, receptoractivity# (5e−40); GO_CC:GO:0016020, membrane# (8e−25) 100 Lipoprotein n= 1 Tax = Zea mays RepID = B6SKL9_MAIZE (5e−60) 3 108 163993324163995044 101 Lysine ketoglutarate reductase trans-splicing related 1 n= 3 Tax = Andropogoneae RepID = B6TPP1_MAIZE (0.0); DUF707: 3 108163995909 164000076 Protein of unknown function (DUF707) (6e−235) 102Protein phosphatase 2c, putative n = 1 Tax = Ricinus communis RepID =B9SVM2_RICCO (2e−78); DUF868: Plant protein of 3 108 164171419 164172810unknown function (DUF868) (6.5e−117); O_MF:GO:0046872, metal ionbinding# (2e−78); GO_BP:GO:0006470, protein amino cid dephosphorylation#(2e−78); GO_CC:GO:0008287, protein serine/threonine phosphatase complex#(2e−78) 103 Putative uncharacterized protein Sb03g043370 n = 1 Tax =Sorghum bicolor RepID = C5XFK5_SORBI (1e−104) 3 108 164174099 164192802104 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6TXB0_MAIZE (0.0); MRP-S28: Mitochondrial ribosomal subunit protein(2.4e−08); 3 108 164251551 164255647 GO_CC:GO:0005829, DA#cytosol#(1e−120) 105 Putative uncharacterized protein Sb10g021032 (Fragment) n =1 Tax = Sorghum bicolor RepID = C5Z404_SORBI (4e−65) 3 108 164319175164320946 106 Retrotransposon gag protein n = 1 Tax = Asparagusofficinalis RepID = Q2AA53_ASPOF (9e−49); Retrotrans_gag:Retrotransposon gag protein (2.3e−09); 3 108 164343946 164347214GO_MF:GO:0004523, ribonuclease H activity# (1e−44); GO_BP:GO:0015074,DNA integration# (1e−44); GO_CC:GO:0005634, nucleus# (1e−44) 107OSJNBa0091D06.8 protein n = 1 Tax = Oryza sativa RepID = Q7XU13_ORYSA(2e−30); IRF: Interferon regulatory factor transcription factor (0.1); 3108 164347526 164348056 GO_MF:GO:0004523, ribonuclease H activity#(1e−30); GO_BP:GO:0015074, DNA integration# (1e−30); GO_CC:GO:0005634,nucleus# (2e−30) 108 Endoribonuclease Dicer homolog 3a n = 1 Tax = Oryzasativa Japonica Group RepID = DCL3A_ORYSJ (0.0); Helicase_C: Helicaseconserved 3 108 164365769 164375526 C-terminal domain (7.3e−18); dsrm:Double-stranded RNA binding motif (0.032); dsRNA_bind: Double strandedRNA binding domain (1.2e−18); PAZ: PAZ domain (1.8e−07); Ribonuclease_3:RNase3 domain (8.5e−34); Ribonuclease_3: RNase3 domain (2.6e−43); dsrm:Double-stranded RNA binding motif (0.23); dsrm: Double-stranded RNAbinding motif (0.31); GO_MF:GO:0046872, metal ion binding# (0.0);GO_BP:GO:0031047, IMP#gene silencing by RNA# (0.0); GO_CC:GO:0005634,nucleus# (0.0) 109 HD2 type histone deacetylase HDA106 n = 1 Tax = Zeamays RepID = Q94F81_MAIZE (1e−109); GO_MF:GO:0046872, metal ion binding#(1e−28); 3 108 164377535 164380957 GO_BP:GO:0045449, regulation oftranscription# (1e−28); GO_CC:GO:0005730, IDA#nucleolus# (1e−28) 110Harpin-induced protein n = 1 Tax = Zea mays RepID = B6UDA2_MAIZE(1e−124); Hin1: Harpin-induced protein 1 (Hin1) (7.5e−27) 3 108164390945 164392443 111 Integrator complex subunit 9 homolog n = 1 Tax =Nematostella vectensis RepID = INT9_NEMVE (8e−38); GO_MF:GO:0005515,protein binding# (1e−32); 3 108.1 164476703 164482132 GO_BP:GO:0016180,snRNA processing# (1e−32); GO_CC:GO:0005634, nucleus# (8e−38) 112Mitochondrial transcription termination factor-like n = 2 Tax = Oryzasativa Japonica Group RepID = Q67UH1_ORYSJ (2e−79); mTERF: mTERF(5.4e−09); 3 108.1 164502626 164504224 GO_MF:GO:0005524, ATP binding#(5e−71); GO_BP:GO:0006468, protein amino acid phosphorylation# (5e−71)113 Copine III-like n = 3 Tax = Oryza sativa RepID = Q5N6Z8_ORYSJ (0.0);Copine: Copine (3e−93); zf-P11: P-11 zinc finger (0.093); zf-C3HC4: 3108.1 164580643 164584828 Zinc finger, C3HC4 type (RING finger)(0.0026); GO_MF:GO:0046872, metal ion binding# (0.0); GO_BP:GO:0009850,IMP#auxin metabolic process# (1e−156); GO_CC:GO:0016020, membrane#(1e−156) 114 Proline transport protein-like n = 2 Tax = Oryza sativaRepID = Q8L431_ORYSJ (0.0); Aa_trans: Transmembrane amino acidtransporter protein (3e−50); 3 108.1 164586510 164589297GO_MF:GO:0015193, IGI#L-proline transmembrane transporter activity#(1e−89); GO_BP:GO:0015824, proline transport# (1e−89); GO_CC:GO:0016021,integral to membrane# (0.0) 115 Putative uncharacterized protein n = 1Tax = Zea mays RepID = B6SP05_MAIZE (2e−14) 3 108.1 164663433 164687249116 Retrotransposon protein, putative, unclassified n = 1 Tax = Oryzasativa Japonica Group RepID = Q2QUP3_ORYSJ (4e−40); zf-CCHC: Zincknuckle 3 108.1 164765422 164771885 (3.8e−05); zf-CCHC: Zinc knuckle(4.2e−06); GO_MF:GO:0003964, RNA-directed DNA polymerase, group IIintron encoded# (4e−40); GO_BP:GO:0006278, RNA-dependent DNAreplication# (4e−40) 117 Hsp70 molecular chaperone (Fragment) n = 72 Tax= Bradyrhizobium RepID = A5A9H9_9BRAD (2e−23); GO_MF:GO:0005524, ATPbinding# (8e−26); 3 108.1 164773452 164773959 GO_BP:GO:0006950, responseto stress# (4e−25); GO_CC:GO:0005739, mitochondrion# (4e−23) 118P0497A05.17 protein n = 2 Tax = Oryza sativa RepID = Q8L4S2_ORYSJ(1e−116); CorA: CorA-like Mg2+ transporter protein (2.4e−31); 3 108.15164884487 164898704 GO_MF:GO:0046873, metal ion transmembranetransporter activity# (8e−66); GO_BP:GO:0055085, transmembranetransport# (8e−66); GO_CC:GO:0016020, membrane# (8e−66) 119 HAT familydimerisation domain containing protein n = 3 Tax = Oryza sativa JaponicaGroup RepID = Q2QPA8_ORYSJ (5e−11); GO_MF:GO:0046983, 3 108.2 164907013164907497 protein dimerization activity# (3e−15); GO_BP:GO:0005975,carbohydrate metabolic process# (1e−13); GO_CC:GO:0005622,intracellular# (1e−13) 120 Putative uncharacterized protein Sb02g031755(Fragment) n = 1 Tax = Sorghum bicolor RepID = C5X711_SORBI (1e−40);GO_MF:GO:0003677, 3 108.2 164918544 164918859 DNA binding# (4e−36);GO_BP:GO:0015074, DNA integration# (2e−09) 121 Putative uncharacterizedprotein n = 2 Tax = Zea mays RepID = B4FIS6_MAIZE (1e−22);zf-C2HC_plant: Protein of unknown function, DUF1544 (2.5e−15); 3 108.2164918884 164960006 GO_MF:GO:0003677, DNA binding# (1e−22) 122Protein-S-isoprenylcysteine O-methyltransferase n = 2 Tax = Zea maysRepID = B6TWA2_MAIZE (1e−107); ICMT: Isoprenylcysteine carboxyl 3 108.2164967420 164969981 methyltransferase (ICMT) family (6e−39);GO_MF:GO:0016740, transferase activity# (1e−107); GO_BP:GO:0006481,C-terminal protein amino acid methylation# (1e−107); GO_CC:GO:0016021,integral to membrane# (1e−107) 123 Protein binding protein, putative n =1 Tax = Ricinus communis RepID = B9RAQ1_RICCO (2e−56); zf-TAZ: TAZ zincfinger (0.099); GO_MF:GO:0008270, 3 108.2 164974619 164978606 zinc ionbinding# (1e−114); GO_BP:GO:0006355, regulation of transcription,DNA-dependent# (1e−114); GO_CC:GO:0005634, nucleus# (1e−114) 124Putative uncharacterized protein Sb05g019060 n = 1 Tax = Sorghum bicolorRepID = C5Y2V6_SORBI (6e−18) 3 108.2 165056085 165059577 125 Proteinterminal ear1 n = 1 Tax = Zea mays RepID = TE1_MAIZE (0.0); RRM_1: RNArecognition motif. (a.k.a. RRM, RB (0.0065); RRM_1: 3 108.2 165174172165178071 RNA recognition motif. (a.k.a. RRM, RB (0.00023); RRM_2: RNArecognition motif 2 (4e−39); GO_MF:GO:0003723, RNA binding# (0.0);GO_BP:GO:0007275, TAS#multicellular organismal development# (0.0) 126B1358B12.21 protein n = 4 Tax = Oryza sativa RepID = Q7XUS3_ORYSJ(3e−22); GO_MF:GO:0004803, transposase activity# (3e−21);GO_BP:GO:0006313, 3 108.3 165300798 165301802 transposition,DNA-mediated# (3e−21) 127 Putative polyprotein n = 1 Tax = Oryza sativaJaponica Group RepID = Q6UUL5_ORYSJ (6e−39); GO_MF:GO:0003700,transcription factor activity# (6e−39); 3 108.3 165394588 165395472GO_BP:GO:0045449, regulation of transcription# (6e−39) 128 P0497A05.6protein n = 1 Tax = Oryza sativa Japonica Group RepID = Q8LIW5_ORYSJ(2e−14) 3 108.4 165456958 165457869 129 Nucleic acid binding protein,putative n = 1 Tax = Ricinus communis RepID = B9RAP0_RICCO (1e−140);JmjN: jmjN domain (1.3e−15); JmjC: 3 108.5 165450429 165455337 JmjCdomain (5e−54); DUF1126: Repeat of unknown function (DUF1126) (0.019);GO_MF:GO:0008270, zinc ion binding# (0.0); GO_BP:GO:0048366, TAS#leafdevelopment# (1e−139); GO_CC:GO:0005622, intracellular# (0.0) 130 BCL-2binding anthanogene-1 n = 3 Tax = Andropogoneae RepID = B6TXB6_MAIZE(2e−66); ubiquitin: Ubiquitin family (0.014); GO_MF:GO:0005515, protein3 108.5 165460350 165461480 binding# (2e−26); GO_BP:GO:0006915,apoptosis# (2e−26) 131 P0497A05.3 protein n = 3 Tax = Oryza sativa RepID= Q8LIW8_ORYSJ (0.0); TPR_1: Tetratricopeptide repeat (0.0011); TPR_2:Tetratricopeptide repeat 3 108.5 165463587 165478472 (8.1e−05); TPR_4:Tetratricopeptide repeat (0.87); TPR_1: Tetratricopeptide repeat (0.42);TPR_2: Tetratricopeptide repeat (0.39); TPR_1: Tetratricopeptide repeat(0.015); TPR_2: Tetratricopeptide repeat (0.002); TPR_1:Tetratricopeptide repeat (2e−06); TPR_2: Tetratricopeptide repeat(1.4e−06); TPR_4: Tetratricopeptide repeat (9.2); TPR_1:Tetratricopeptide repeat (3.4); TPR_2: Tetratricopeptide repeat (1.3);TPR_1: Tetratricopeptide repeat (4.7); TPR_2: Tetratricopeptide repeat(26); TPR_1: Tetratricopeptide repeat (0.0017); TPR_2: Tetratricopeptiderepeat (0.006); TPR_1: Tetratricopeptide repeat (0.0017); TPR_2:Tetratricopeptide repeat (0.00013); TPR_4: Tetratricopeptide repeat(2.8); GO_MF:GO:0005488, binding# (0.0); GO_BP:GO:0006508, proteolysis#(7e−21); GO_CC:GO:0005622, intracellular# (7e−21) 132 Ceramideglucosyltransferase, putative n = 1 Tax = Ricinus communis RepID =B9RKH1_RICCO (2e−20); GO_MF:GO:0016757, 3 108.55 165653906 165669525transferase activity, transferring glycosyl groups# (4e−21) 133 Cysteineendopeptidase n = 5 Tax = Oryza sativa RepID = Q7F3A8_ORYSJ (1e−148);Inhibitor_I29: Cathepsin propeptide inhibitor domain 3 108.6 165445237165446893 ((2.9e−14); DUF1918: Domain of unknown function (DUF1918)(0.073); Peptidase C1: Papain family cysteine protease (1.1e−129);GO_MF:GO:0016787, hydrolase activity# (1e−177); GO_BP:GO:0006508,proteolysis# (1e−177); GO_CC:GO:0005788, endoplasmic reticulum lumen#(1e−117) 134 Nucleic acid binding protein, putative n = 1 Tax = Ricinuscommunis RepID = B9RAP0_RICCO (1e−15); GO_MF:GO:0008270, zinc ionbinding# (3e−33); 3 108.6 165448724 165450566 GO_BP:GO:0048366, TAS#leafdevelopment# (6e−12); GO_CC:GO:0005622, intracellular# (3e−33) 135Putative uncharacterized protein n = 2 Tax = Zea mays RepID =Q5GAU8_MAIZE (7e−17) 3 108.6 165551858 165552144 136 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C0PLF6_MAIZE(1e−13) 3 108.6 165668072 165668383 137 Fructose-bisphosphate aldolasecytoplasmic isozyme n = 18 Tax = commelinids RepID = ALF_ORYSJ (0.0);Glycolytic: Fructose-bisphosphate aldolase class-I 3 108.8 165723021165725581 (2.1e−259); GO_MF:GO:0016829, lyase activity# (0.0);GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0005737, cytoplasm#(0.0) 138 PSRP4 n = 2 Tax = Andropogoneae RepID = B6T2D1_MAIZE (8e−18) 3108.8 165725766 165728006 139 Protein binding protein, putative n = 1Tax = Ricinus communis RepID = B9RMQ0_RICCO (4e−68); zf-C3HC4: Zincfinger, C3HC4 type (RING finger) (0.0001); 3 108.8 165728771 165731884GO_MF:GO:0046872, metal ion binding# (1e−122) 140 Putativeuncharacterized protein Sb03g043110 n = 1 Tax = Sorghum bicolor RepID =C5XFH4_SORBI (0.0); B3: B3 DNA binding domain 3 109 165855198 165857792(1.4e−10); B3: B3 DNA binding domain (0.00015); GO_MF:GO:0003677, DNAbinding# (1e−125); GO_BP:GO:0045449, regulation of transcription#(1e−125); GO_CC:GO:0005634, nucleus# (1e−125) 141 Exo70 exocyst complexsubunit family protein n = 2 Tax = Zea mays RepID = B6SWM6_MAIZE (0.0);Exo70: Exo70 exocyst complex subunit (7.5e−78); 3 109 165857827165860040 GO_BP:GO:0006887, exocytosis# (0.0); GO_CC:GO:0000145,NAS#exocyst# (0.0) 142 Putative transposase n = 1 Tax = Oryza sativaJaponica Group RepID = Q8L516_ORYSJ (1e−84); Plant_tran: Planttransposon protein (5e−05); 3 109.1 165874313 165876275GO_MF:GO:0005524, ATP binding# (1e−78); GO_BP:GO:0006468, protein aminoacid phosphorylation# (1e−78) 143 Two-component response regulator ARR11n = 2 Tax = Andropogoneae RepID = B6UC09_MAIZE (0.0); Response_reg:Response regulator 3 109.1 165897223 165901241 receiver domain(1.2e−27); Myb_DNA-binding: Myb-like DNA-binding domain (7.9e−11);GO_MF:GO:0003677, DNA binding# (0.0); GO_BP:GO:0045449, regulation oftranscription# (0.0); GO_CC:GO:0005634, nucleus# (0.0) 144 Putativeuncharacterized protein Sb03g043070 n = 1 Tax = Sorghum bicolor RepID =C5XFH0_SORBI (0.0) 3 109.1 165903035 165912074 145 E3 SUMO-proteinligase SIZ2 n = 2 Tax = Oryza sativa RepID = SIZ2_ORYSJ (3e−11);GO_MF:GO:0046872, metal ion binding# (3e−11); 3 109.1 165921011165921870 GO_CC:GO:0005634, nucleus# (3e−11) 146 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C4IYH0_MAIZE(2e−21) 3 109.3 165985310 165986062 147 B1065G12.33 protein n = 4 Tax =Oryza sativa RepID = Q8RZ62_ORYSJ (4e−32); DUF971: Protein of unknownfunction (DUF971) (2.3e−41); 3 109.3 166019231 166033766GO_MF:GO:0016853, isomerase activity# (3e−21) 148 Protein kinase n = 3Tax = Andropogoneae RepID = B6SS49_MAIZE (0.0); ABC1: ABC1 family(2.9e−43); GO_MF:GO:0020037, heme binding# (0.0); 3 109.3 166071796166094047 GO_BP:GO:0016301, kinase activity# (0.0); GO_CC:GO:0005886,plasma membrane# (8e−39) 149 Gibberellin response modulator-like proteinn = 2 Tax = Oryza sativa RepID = Q8RZ73_ORYSJ (1e−174); GRAS: GRASfamily transcription factor (1.2e−119); 3 109.5 166151703 166153319GO_MF:GO:0005515, protein binding# (2e−47); GO_BP:GO:0045449, regulationof transcription# (1e−174); GO_CC:GO:0005634, nucleus# (8e−50) 150Transposon protein, putative, CACTA, En/Spm sub-class n = 1 Tax = Oryzasativa Japonica Group RepID = Q2QWY8_ORYSJ (3e−59); GO_MF:GO:0004803, 3109.7 166196959 166197693 transposase activity# (3e−36);GO_BP:GO:0006313, transposition, DNA-mediated# (3e−36) 151 Peptidetransporter PTR2 n = 2 Tax = Zea mays RepID = B6SXM6_MAIZE (0.0); MFS_1:Major Facilitator Superfamily (5.7e−05); PTR2: POT family (2.4e−118); 3109.7 166199666 166202898 GO_MF:GO:0005215, transporter activity# (0.0);GO_BP:GO:0006857, oligopeptide transport# (0.0); GO_CC:GO:0016021,integral to membrane# (0.0) 152 B1065G12.16 protein n = 2 Tax = Oryzasativa RepID = Q8RZ79_ORYSJ (0.0); Abhydrolase_3: alpha/beta hydrolasefold (3.1e−75); 3 109.8 166243856 166246747 GO_MF:GO:0016787, hydrolaseactivity# (0.0); GO_BP:GO:0008152, metabolic process# (0.0);GO_CC:GO:0005634, nucleus# (9e−30) 153 Endopeptidase-like protein n = 1Tax = Oryza sativa Japonica Group RepID = Q8RZ80_ORYSJ (0.0);Peptidase_M3: Peptidase family M3 (1.1e−91); 3 109.85 166247016166254397 GO_MF:GO:0016787, hydrolase activity# (0.0); GO_BP:GO:0006508,proteolysis# (0.0); GO_CC:GO:0005737, cytoplasm# (1e−109) 154 Acidphosphatase/vanadium-dependent haloperoxidase related n = 3 Tax =Andropogoneae RepID = B6SYG4_MAIZE (2e−52); 3 110 166377306 166379640DUF212: Divergent PAP2 family (7.6e−44); GO_MF:GO:0004601, peroxidaseactivity# (2e−52) 155 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = B6SGD8_MAIZE (9e−28) 3 110.1 166319780 166320127 156 VTC2 n= 2 Tax = Zea mays RepID = B6T940_MAIZE (0.0); GO_MF:GO:0080048,IDA#GDP-D-glucose phosphorylase activity# (1e−105); 3 110.3 166381511166384098 GO_BP:GO:0019853, L-ascorbic acid biosynthetic process#(1e−105) 157 DNA repair protein recA n = 3 Tax = Andropogoneae RepID =B6TNM4_MAIZE (0.0); RecA: recA bacterial DNA recombination protei(4.6e−179); 3 110.3 166384292 166391067 Rad51: Rad51 (8e−05); MipZ:ATPase MipZ (0.089); GO_MF:GO:0017111, nucleoside-triphosphataseactivity# (0.0); GO_BP:GO:0009432, SOS response# (0.0);GO_CC:GO:0005737, cytoplasm# (0.0) 158 B1065G12.5 protein n = 1 Tax =Oryza sativa Japonica Group RepID = Q7F1X9_ORYSJ (0.0); U-box: U-boxdomain (1.6e−20); Arm: 3 110.3 166431215 166436430Armadillo/beta-catenin-like repeat (0.24); Arm:Armadillo/beta-catenin-like repeat (1.7); Arm:Armadillo/beta-catenin-like repeat (2.2); GO_MF:GO:0005488, binding#(0.0); GO_BP:GO:0016567, IGI#protein ubiquitination# (0.0);GO_CC:GO:0000151, ubiquitin ligase complex# (0.0) 159 Cysteine-typepeptidase, putative n = 1 Tax = Ricinus communis RepID = B9T7Q4_RICCO(3e−54); OTU: OTU-like cysteine protease (2.1e−30); 3 110.6 166446402166449258 GO_MF:GO:0016874, ligase activity# (1e−56); GO_BP:GO:0009058,biosynthetic process# (1e−56) 160 Helix-loop-helix DNA-binding domaincontaining protein n = 1 Tax = Zea mays RepID = B6TUA5_MAIZE (1e−160);HLH: Helix-loop-helix 3 110.6 166448058 166452009 DNA-binding domain(3.4e−08); GO_MF:GO:0030528, transcription regulator activity# (1e−160);GO_BP:GO:0045449, regulation of transcription# (1e−160);GO_CC:GO:0005634, nucleus# (1e−160) 161 Transposon protein Pongsub-class n = 1 Tax = Zea mays RepID = B6TCG7_MAIZE (0.0); Plant_tran:Plant transposon protein (4.3e−56); 3 110.7 166486192 166487676GO_MF:GO:0016740, transferase activity# (1e−105); GO_CC:GO:0005840,ribosome# (1e−107) 162 L-asparaginase 2 n = 1 Tax = Phaseolus vulgarisRepID = Q2PW34_PHAVU (2e−19); GO_MF:GO:0016787, hydrolase activity#(6e−57) 3 110.8 166526151 166529874 163 Putative DAD1 n = 3 Tax = Oryzasativa RepID = Q8S1D9_ORYSJ (0.0); Lipase_3: Lipase (class 3) (5.3e−53);Thioesterase: Thioesterase domain (0.096); 3 110.9 166596752 166598153GO_MF:GO:0004806, triglyceride lipase activity# (1e−171);GO_BP:GO:0006629, lipid metabolic process# (1e−171); GO_CC:GO:0009507,chloroplast# (1e−108) 164 Glycosyltransferase n = 3 Tax = AndropogoneaeRepID = Q5QPY6_SORBI (0.0); DUF563: Protein of unknown function (DUF563)(1.5e−133); 3 110.9 166689883 166692410 GO_MF:GO:0016757, transferaseactivity, transferring glycosyl groups# (0.0) 165 Streptococcalhemagglutinin-like protein n = 2 Tax = Oryza sativa Japonica Group RepID= Q6EN70_ORYSJ (1e−116); DUF566: Family of 3 110.9 166997433 167001364unknown function (DUF566) (9.5e−81); GO_MF:GO:0008017, IDA#microtubulebinding# (3e−27); GO_BP:GO:0051301, cell division# (3e−27);GO_CC:GO:0005880, IDA#nuclear microtubule# (3e−27) 166S-adenosylmethionine-dependent methyltransferase, putative n = 1 Tax =Ricinus communis RepID = B9SX25_RICCO (1e−128); DUF248: 3 110.9167004322 167007358 Putative methyltransferase (9.1e−213);methyltransf_11: Methyltransferase domain (0.00061); GO_MF:GO:0016740,transferase activity# (1e−128); GO_BP:GO:0016301, kinase activity#(1e−119); GO_CC:GO:0005794, IDA#Golgi apparatus# (1e−112) 167 PutativePto kinase interactor 1 n = 1 Tax = Oryza sativa Japonica Group RepID =Q69IN5_ORYSJ (1e−138); Pkinase_Tyr: 3 110.9 167008810 167013529 Proteintyrosine kinase (2.6e−25); Pkinase: Protein kinase domain (2.6e−19);GO_MF:GO:0005524, ATP binding# (1e−169); GO_BP:GO:0006468, protein aminoacid phosphorylation# (1e−169); GO_CC:GO:0005886, plasma membrane#(1e−139) 168 Probable mannan synthase 2 n = 1 Tax = Oryza sativaJaponica Group RepID = CSLA2_ORYSJ (1e−26); GO_MF:GO:0016757, 3 110.9167017762 167018749 transferase activity, transferring glycosyl groups#(1e−26); GO_BP:GO:0007047, cellular cell wall organization# (1e−26);GO_CC:GO:0016021, integral to membrane# (1e−26) 169 OSJNBa0071G03.3protein n = 3 Tax = Oryza sativa RepID = Q7XX26_ORYSJ (8e−14); DUF1685:Protein of unknown function (DUF1685) (1.7e−34) 3 110.9 167049453167052175 170 Ovule development aintegumenta-like protein BNM3 n = 1 Tax= Oryza sativa RepID = Q8LGQ3_ORYSA (1e−135); AP2: AP2 domain (1.2e−09);3 110.95 166793205 166797397 AP2: AP2 domain (1.6e−14);GO_MF:GO:0003700, transcription factor activity# (1e−128);GO_BP:GO:0045449, regulation of transcription# (1e−128);GO_CC:GO:0005634, nucleus# (1e−128) 171 diacylglycerol lipase beta n = 2Tax = Gallus gallus RepID = UPI0000ECAA58 (4e−12); Lipase 3: Lipase(class 3) (3.3e−06); GO_MF:GO:0004806, 3 111 166663041 166670528triglyceride lipase activity# (0.0); GO_BP:GO:0006629, lipid metabolicprocess# (0.0) 172 Protein binding protein, putative n = 1 Tax = Ricinuscommunis RepID = B9S8S6_RICCO (1e−18); GO_MF:GO:0005515, proteinbinding# (3e−55) 3 111 166675147 166675905 173 Putative uncharacterizedprotein Sb03g042790 n = 3 Tax = Andropogoneae RepID = C5XFE3_SORBI (0.0)3 111 166811247 166817387 174 Putative uncharacterized proteinSb03g042800 n = 1 Tax = Sorghum bicolor RepID = C5XFE4_SORBI (3e−78);Pollen_Ole_e_I: Pollen 3 111.05 166798399 166800620 proteins Ole e Ifamily (5.7e−10); Extensin_2: Extensin-like region (0.48); Extensin_2:Extensin-like region (0.65); Extensin_2: Extensin-like region (0.4) 175Patatin, putative n = 1 Tax = Ricinus communis RepID = B9R7G2_RICCO(1e−76); Patatin: Patatin-like phospholipase (4.1e−05);GO_MF:GO:0016787, 3 111.05 167072867 167075108 hydrolase activity#(1e−177); GO_BP:GO:0008152, metabolic process# (1e−177);GO_CC:GO:0016020, membrane# (6e−60) 176 Putative integral membraneprotein n = 1 Tax = Zea mays RepID = Q5GAV3_MAIZE (1e−27);GO_MF:GO:0016758, transferase activity, transferring 3 111.1 166801413166807981 hexosyl groups# (6e−31); GO_BP:GO:0008152, metabolic process#(6e−31); GO_CC:GO:0016020, membrane# (1e−27) 177 Kiaa0078 protein(Fragment) n = 1 Tax = Oryza sativa RepID = Q9XFD8_ORYSA (6e−92);Rad21_Rec8_N: N terminus of Rad21/Rec8 like 3 111.2 167362932 167372276protein (3.1e−66); Rad21_Rec8: Conserved region of Rad21/Rec8 likeprotein (4.6e−14); GO_MF:GO:0005515, protein binding# (2e−33);GO_BP:GO:0007062, NAS#sister chromatid cohesion# (1e−83);GO_CC:GO:0000228, nuclear chromosome# (0.0) 178 Formin-like protein 1 n= 1 Tax = Oryza sativa Japonica Group RepID = FH1_ORYSJ (0.0); FH2:Formin Homology 2 Domain (1.8e−167); GO_MF:GO:0003779, 3 111.4 167430557167434529 actin binding# (0.0); GO_BP:GO:0030036, actin cytoskeletonorganization# (0.0); GO_CC:GO:0016021, integral to membrane# (0.0) 179Beta-glucosidase 4 n = 2 Tax = Oryza sativa RepID = BGL04_ORYSJ (8e−45);GO_MF:GO:0043169, cation binding# (3e−51); GO_BP:GO:0005975, 3 111.75167437055 167437918 carbohydrate metabolic process# (3e−51);GO_CC:GO:0022626, IDA#cytosolic ribosome# (1e−22) 180 Beta-glucosidase 4n = 2 Tax = Oryza sativa RepID = BGL04_ORYSJ (0.0); Glyco_hydro_1:Glycosyl hydrolase family 1 (1.6e−130); GO_MF:GO:0043169, 3 111.8167442292 167446542 cation binding# (0.0); GO_BP:GO:0005975,carbohydrate metabolic process# (0.0); GO_CC:GO:0005576, extracellularregion# (2e−98) 181 Retrotransposon protein, putative, Tyl-copiasubclass n = 1 Tax = Oryza sativa Japonica Group RepID = Q2QSG2_ORYSJ(4e−55); Retrotrans_gag: Retrotransposon 3 111.8 167449115 167449852 gagprotein (0.0017); GO_MF:GO:0003677, DNA binding# (4e−55);GO_BP:GO:0015074, DNA integration# (4e−55) 182 Pentatricopeptide repeatprotein n = 2 Tax = Oryza sativa RepID = A0JBX0_ORYSJ (1e−142); PPR: PPRrepeat (0.14); PPR: PPR repeat (0.82); 3 111.8 167472698 167474938 PPR:PPR repeat (0.62); PPR: PPR repeat (0.00036); PPR: PPR repeat (5.4e−07);PPR: PPR repeat (7.9e−07); PPR: PPR repeat (2e−11); GO_MF:GO:0005488,binding# (1e−142); GO_BP:GO:0006952, defense response# (1e−26);GO_CC:GO:0005739, mitochondrion# (2e−99) 183 50S ribosomal protein L13 n= 2 Tax = Andropogoneae RepID = B6TQ75_MAIZE (4e−09); GO_MF:GO:0003735,structural constituent of ribosome# (3e−09); 3 111.8 167479089 167479298GO_BP:GO:0006412, translation# (3e−09); GO_CC:GO:0005840, ribosome#(3e−09) 184 50S ribosomal protein L13 n = 2 Tax = Andropogoneae RepID =B6TQ75_MAIZE (1e−49); Ribosomal_L13: Ribosomal protein L13 (2.3e−07); 3111.8 167486399 167488255 GO_MF:GO:0003735, structural constituent ofribosome# (1e−49); GO_BP:GO:0006412, translation# (1e−49);GO_CC:GO:0005840, ribosome# (1e−49) 185 Ribonuclease 2 n = 2 Tax = Zeamays RepID = B6TDK4_MAIZE (1e−149); Ribonuclease_T2: Ribonuclease T2family (8.8e−55); GO_MF:GO:0033897, 3 111.8 167682817 167685987ribonuclease T2 activity# (1e−149); GO_BP:GO:0006950, response tostress# (2e−60); GO_CC:GO:0005773, IDA#vacuole# (2e−60) 186 Ribonuclease2 n = 2 Tax = Zea mays RepID = B6TGK1_MAIZE (1e−146); Ribonuclease_T2:Ribonuclease T2 family (3.1e−57); GO_MF:GO:0033897, 3 111.8 167686841167690777 ribonuclease T2 activity# (1e−146); GO_BP:GO:0006950, responseto stress# (6e−69); GO_CC:GO:0005773, IDA#vacuole# (6e−69) 187Glycosyltransferase n = 1 Tax = Populus trichocarpa RepID = B9N4D7_POPTR(1e−120); Glyco_transf_8: Glycosyl transferase family 8 3 111.8167711919 167713615 (1.5e−38); GO_MF:GO:0016757, transferase activity,transferring glycosyl groups# (1e−137); GO_BP:GO:0006468, protein aminoacid phosphorylation# (3e−58); GO_CC:GO:0005886, plasma membrane#(6e−65) 188 OSJNBa0063C18.6 protein n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q7X6Q9_ORYSJ (3e−14); GO_MF:GO:0008234, cysteine-type 3111.8 167715238 167717735 peptidase activity# (3e−14); GO_BP:GO:0006508,proteolysis# (3e−14); GO_CC:GO:0030529, ribonucleoprotein complex#(5e−10) 189 DNA helicase homolog, putative n = 1 Tax = Musa acuminataRepID = Q1EPC6_MUSAC (0.0); DUF889: Eukaryotic protein of unknown 3111.8 167729335 167732914 function (DUF889) (7.1e−70); GO_MF:GO:0004386,helicase activity# (0.0) 190 Helicase-like protein n = 1 Tax = Oryzasativa Japonica Group RepID = Q9AYF0_ORYSJ (7e−24); GO_MF:GO:0004386,helicase activity# (7e−24) 3 111.8 167732923 167734044 191 Sec20 familyprotein n = 4 Tax = Andropogoneae RepID = B6TQD1_MAIZE (4e−46) 3 111.8167734979 167735405 192 MPPN domain containing protein n = 1 Tax = Zeamays RepID = B6UAH6_MAIZE (4e−17); Sec20: Sec20 (0.03); MPPN: MPPN(rrm-like) domain (0.022); 3 111.8 167744313 167750012 GO_MF:GO:0004576,oligosaccharyl transferase activity# (1e−12); GO_BP:GO:0006486, proteinamino acid glycosylation# (1e−12); GO_CC:GO:0016020, membrane# (1e−12)193 P0696G06.27 protein n = 2 Tax = Oryza sativa RepID = Q8L4W8_ORYSJ(0.0); Rhodanese: Rhodanese-like domain (0.0041); GO_MF:GO:0004872, 3111.8 168290364 168298568 receptor activity# (1e−14); GO_BP:GO:0004872,receptor activity# (1e−14); GO_CC:GO:0009507, chloroplast# (4e−63) 194Chromatin assembly factor-1 n = 3 Tax = Oryza sativa RepID =B2ZX90_ORYSJ (0.0); TolA: TolA protein (0.0032); DUF1154: Protein ofunknown 3 111.8 168301241 168308101 function (DUF1154) (0.023);GO_MF:GO:0005524, ATP binding# (6e−36); GO_BP:GO:0048366, TAS#leafdevelopment# (8e−91); GO_CC:GO:0005678, IPI#chromatin assembly complex#(8e−91) 195 Putative mutator-like transposase n = 2 Tax = Oryza sativaJaponica Group RepID = Q75IN8_ORYSJ (1e−27) 3 111.8 168301382 168301870196 Putative ribosomal protein n = 1 Tax = Oryza sativa RepID =Q8SB40_ORYSA (5e−48); Plant_tran: Plant transposon 3 111.8 168363189168363859 protein (5.9e−23); GO_MF:GO:0016740, transferase activity#(5e−25); GO_CC:GO:0005840, ribosome# (5e−48) 197 Putative ribosomalprotein n = 1 Tax = Oryza sativa RepID = Q8SB40_ORYSA (3e−09);GO_CC:GO:0005840, ribosome# (3e−09) 3 111.8 168363866 168364830 198Putative uncharacterized protein n = 2 Tax = Zea mays RepID =B6TTS0_MAIZE (3e−46) 3 111.8 168429303 168429952 199 ATP binding proteinn = 3 Tax = Andropogoneae RepID = B6SYS4_MAIZE (3e−13); DUF1296: Proteinof unknown function (DUF1296) (0.0017); 3 111.8 168430535 168434782GO_MF:GO:0005524, ATP binding# (3e−13); GO_BP:GO:0006468, protein aminoacid phosphorylation# (3e−13) 200 Alpha-2,8-sialyltransferase 8b,putative n = 1 Tax = Ricinus communis RepID = B9RA86_RICCO (1e−70);GO_MF:GO:0008373, 3 111.8 168438932 168440345 sialyltransferaseactivity# (1e−103); GO_BP:GO:0006486, protein amino acid glycosylation#(1e−103); GO_CC:GO:0030173, integral to Golgi membrane# (1e−103) 201Zinc finger protein n = 1 Tax = Zea mays RepID = B6SJ02_MAIZE (1e−80);Trigger_N: Bacterial trigger factor protein 3 111.8 168480872 168487707(TF) (0.019); zf-C3HC4: Zinc finger, C3HC4 type (RING finger) (2.2e−15);GO_MF:GO:0008270, zinc ion binding# (1e−80); GO_BP:GO:0015031, proteintransport# (1e−109); GO_CC:GO:0009570, IDA#chloroplast stroma# (1e−21)202 Selenoprotein n = 4 Tax = Andropogoneae RepID = B6T325_MAIZE(5e−81); Sep15_SelM: Sep15/SelM redox domain (6.7e−40); 3 111.8168489946 168492960 GO_MF:GO:0008430, selenium binding# (9e−16);GO_BP:GO:0051084, IDA#‘de novo’ posttranslational protein folding#(9e−16); GO_CC:GO:0005788, endoplasmic reticulum lumen# (9e−16) 203Fructokinase-1 n = 8 Tax = Poaceae RepID = SCRK1_ORYSJ (1e−166); PfkB:pfkB family carbohydrate kinase (4.3e−107); GO_MF:GO:0016740, 3 111.8168523210 168526249 transferase activity# (1e−166); GO_BP:GO:0016301,kinase activity# (1e−166); GO_CC:GO:0005886, plasma membrane# (1e−136)204 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B8A0T9_MAIZE (1e−38) 3 111.9 167786045 167789878 205 Retrotransposonprotein, putative, unclassified n = 1 Tax = Oryza sativa Japonica GroupRepID = Q1OPF2_ORYSJ (0.0); zf-H2C2: His(2)-Cys(2) zinc finger 3 111.9167790427 167792988 (0.011); rve: Integrase core domain (6.9e−18);Chromo: ‘chromo’ (CHRromatin Organisation MOd (5.5e−11);GO_MF:GO:0003964, RNA-directed DNA polymerase, group II intron encoded#(0.0); GO_BP:GO:0015074, DNA integration# (0.0); GO_CC:GO:0005634,nucleus# (0.0) 206 Putative polyprotein, 5′-partial (Fragment) n = 1 Tax= Oryza sativa RepID = Q94GF8_ORYSA (4e−54); RVP_2: Retroviral aspartylprotease 3 111.9 167793124 167793873 (0.00023); GO_MF:GO:0003964,RNA-directed DNA polymerase, group II intron encoded# (2e−56);GO_BP:GO:0015074, DNA integration# (2e−56); GO_CC:GO:0005634, nucleus#(2e−56) 207 Putative uncharacterized protein n = 1 Tax = Zea mays RepID= B8A0T9_MAIZE (2e−73); PPR: PPR repeat (1.9); PPR: PPR repeat (0.089);3 111.9 167797246 167802786 GO_MF:GO:0008270, zinc ion binding# (7e−29);GO_CC:GO:0009507, chloroplast# (7e−29) 208 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = C0PFS4_MAIZE (0.0);GO_MF:GO:0043565, sequence-specific DNA binding# (4e−46); 3 111.9167805773 167810540 GO_BP:GO:0006355, regulation of transcription,DNA-dependent# (4e−46) 209 Membrane-associated 30 kDa protein n = 3 Tax= Andropogoneae RepID = B6T6V3_MAIZE (1e−122); PspA_IM30: PspA/D430family (2.3e−25); 3 111.9 168368220 168373431 Snf7: Snf7 (0.096);GO_BP:GO:0044419, interspecies interaction between organisms# (1e−115);GO_CC:GO:0016020, membrane# (1e−115) 210 HAT family dimerisation domaincontaining protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QRD1_ORYSJ (8e−22); GO_MF:GO:0046983, 3 111.9 168405403 168405915protein dimerization activity# (2e−23) 211 Eukaryotic translationinitiation factor 3 subunit 2 n = 2 Tax = Andropogoneae RepID =B6SSB6_MAIZE (1e−09); GO_MF:GO:0003743, protein-synthesizing 3 111.9168609417 168609826 GTPase activity, initiation# (1e−09);GO_BP:GO:0003743, protein-synthesizing GTPase activity, initiation#(1e−09) 212 AP-3 complex subunit sigma-2 n = 3 Tax = Andropogoneae RepID= B4FA26_MAIZE (4e−26); Clat_adaptor_s: Clathrin adaptor complex smallchain (3.2e−09); 3 111.9 168611405 168613025 GO_MF:GO:0008565, proteintransporter activity# (4e−26); GO_BP:GO:0016192, vesicle-mediatedtransport# (4e−26); GO_CC:GO:0030117, membrane coat# (4e−26) 213Phenylalanine ammonia-lyase n = 22 Tax = Poaceae RepID = PAL2_ORYSJ(4e−46); PAL: Phenylalanine and histidine ammonia-lyase (0.0036); 3111.9 168620337 168620882 GO_MF:GO:0016841, ammonia-lyase activity#(4e−46); GO_BP:GO:0009698, phenylpropanoid metabolic process# (4e−46);GO_CC:GO:0005737, cytoplasm# (4e−46) 214 Leukotriene A-4 hydrolase,putative n = 1 Tax = Ricinus communis RepID = B9SD61_RICCO (1e−17);GO_MF:GO:0016787, hydrolase activity# (6e−36); 3 111.9 168661556168662161 GO_BP:GO:0019370, leukotriene biosynthetic process# (6e−36)215 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =C0PAB7_MAIZE (1e−14) 3 112 167912639 167932679 216 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C0PAB7_MAIZE(7e−18) 3 112 167933809 167934006 217 Putative HOBBIT n = 1 Tax = Oryzasativa Japonica Group RepID = Q69XV2_ORYSJ (0.0); TPR_1:Tetratricopeptide repeat (0.0014); TPR_2: 3 112 168003055 168013349Tetratricopeptide repeat (2.1e−05); TPR_1: Tetratricopeptide repeat(1.5); TPR_2: Tetratricopeptide repeat (5.6); TPR_1: Tetratricopeptiderepeat (9.8e−06); TPR_2: Tetratricopeptide repeat (0.002); TPR_1:Tetratricopeptide repeat (2.6e−05); TPR_2: Tetratricopeptide repeat(0.21); TPR_1: Tetratricopeptide repeat (4e−08); TPR_2:Tetratricopeptide repeat (1.8e−06); TPR_1: Tetratricopeptide repeat(0.013); TPR_2: Tetratricopeptide repeat (0.0028); TPR_1:Tetratricopeptide repeat (3.8); TPR_2: Tetratricopeptide repeat (1.6);TPR_1: Tetratricopeptide repeat (0.12); TPR_2: Tetratricopeptide repeat(0.65); GO_MF:GO:0005488, binding# (0.0); GO_BP:GO:0051510,IGI#regulation of unidimensional cell growth# (0.0); GO_CC:GO:0009504,IDA#cell plate# (0.0) 218 Putative transposable element n = 1 Tax =Oryza sativa Japonica Group RepID = Q8LNC1_ORYSJ (1e−151); MuDR: MuDRfamily transposase 3 112.1 168015671 168017677 (5.6e−27);Transposase_mut: Transposase, Mutator family (0.05); MULE: MULEtransposase domain (2.2e−11); SWIM: SWIM zinc finger (3.4e−08);GO_MF:GO:0008270, zinc ion binding# (0.0); GO_BP:GO:0006313,transposition, DNA-mediated# (0.0) 219 DNA-directed RNA polymerase n = 2Tax = Oryza sativa Japonica Group RepID = Q0DKN9_ORYSJ (2e−35);RNA_pol_Rpb1_2: 3 112.1 168021045 168024149 RNA polymerase Rpb1, domain2 (1.4e−05); GO_MF:GO:0016779, nucleotidyltransferase activity# (2e−35);GO_BP:GO:0006366, transcription from RNA polymerase II promoter#(2e−35); GO_CC:GO:0005665, DNA-directed RNA polymerase II, core complex#(2e−35) 220 Putative uncharacterized protein Sb04g025980 n = 1 Tax =Sorghum bicolor RepID = C5XXM9_SORBI (2e−32) 3 112.1 168854887 168856209221 P0696G06.7 protein n = 3 Tax = Oryza sativa RepID = Q7F447_ORYSJ(0.0); Metallophos: Calcineurin-like phosphoesterase (4.9e−09); 3 112.1168858815 168863872 GO_MF:GO:0016787, hydrolase activity# (0.0);GO_BP:GO:0006396, RNA processing# (0.0); GO_CC:GO:0005622,intracellular# (0.0) 222 Putative uncharacterized protein Sb10g008520 n= 1 Tax = Sorghum bicolor RepID = C5Z7H3_SORBI (1e−10) 3 112.1 168866726168869485 223 Aspartokinase n = 1 Tax = Sorghum bicolor RepID =C5XH02_SORBI (6e−30); AA_kinase: Amino acid kinase family (0.016); 3112.2 168901191 168909642 GO_MF:GO:0016740, transferase activity#(9e−30); GO_BP:GO:0016301, kinase activity# (9e−30); GO_CC:GO:0009536,plastid# (2e−26) 224 Protein binding protein, putative n = 1 Tax =Ricinus communis RepID = B9T840_RICCO (1e−109); BTB: BTB/POZ domain(0.00015); 3 112.2 168918994 168923530 zf-TAZ: TAZ zinc finger (0.0094);GO_MF:GO:0008270, zinc ion binding# (0.0); GO_BP:GO:0006355, regulationof transcription, DNA-dependent# (0.0); GO_CC:GO:0005634, nucleus# (0.0)225 DNA binding protein n = 1 Tax = Zea mays RepID = B6T3S3_MAIZE(3e−28); SRF-TF: SRF-type transcription factor (DNA-binding 3 112.2168925201 168926167 and dimerisation domain) (6.5e−15);GO_MF:GO:0043565, sequence-specific DNA binding# (2e−63);GO_BP:GO:0045449, regulation of transcription# (2e−63);GO_CC:GO:0005634, nucleus# (2e−63) 226 Ankyrin-kinase, putative n = 1Tax = Ricinus communis RepID = B9SV01_RICCO (1e−164); Ank: Ankyrinrepeat (4.3e−11); 3 112.2 168959249 168962676 Pkinase: Protein kinasedomain (7.3e−25); Pkinase_Tyr: Protein tyrosine kinase (9.1e−17);GO_MF:GO:0016301, kinase activity# (0.0); GO_BP:GO:0016301, kinaseactivity# (0.0); GO_CC:GO:0005737, cytoplasm# (1e−95) 227 PAE n = 1 Tax= Litchi chinensis RepID = B3V945_LITCN (6e−50); PAE:Pectinacetylesterase (8.5e−71); GO_MF:GO:0016787, hydrolase activity#(1e−56); 3 112.3 169031162 169033726 GO_CC:GO:0016020, membrane# (2e−50)228 PAE n = 1 Tax = Litchi chinensis RepID = B3V945_LITCN (1e−33); PAE:Pectinacetylesterase (7e−10); GO_MF:GO:0016787, hydrolase activity#(2e−41) 3 112.3 169034338 169035344 229 PAE n = 1 Tax = Litchi chinensisRepID = B3V945_LITCN (1e−92); PAE: Pectinacetylesterase (3.1e−190);GO_MF:GO:0016787, hydrolase activity# (1e−123); 3 112.3 169044021169046721 GO_CC:GO:0016020, membrane# (1e−93) 230 NADP-dependentoxidoreductase P1 n = 3 Tax = Andropogoneae RepID = B6TFG1_MAIZE(1e−110); Glyco_hydro_28: Glycosyl hydrolases family 28 (3.8e−13); 3112.3 169071070 169080582 GO_MF:GO:0016798, hydrolase activity, actingon glycosyl bonds# (0.0); GO_BP:GO:0055114, oxidation reduction# (0.0)231 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6UD01_MAIZE (5e−12) 3 112.3 169081463 169082314 232 Putativetransformer serine/arginine-rich ribonucleoprotein n = 2 Tax = Oryzasativa RepID = Q84QA6_ORYSJ (6e−28); RRM_1: RNA recognition motif. 3112.3 169085515 169087999 (a.k.a. RRM, RB (1.2e−09); GO_MF:GO:0003676,nucleic acid binding# (1e−30); GO_BP:GO:0008380, RNA splicing# (4e−19);GO_CC:GO:0030529, ribonucleoprotein complex# (4e−19) 233 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B4FSH8_MAIZE(2e−51); IQ: IQ calmodulin-binding motif (0.00029); 3 112.35 169093629169095183 IQ: IQ calmodulin-binding motif (0.8) 234 TPR repeat n = 1 Tax= Medicago truncatula RepID = A2Q5X4_MEDTR (4e−41); TPR_2:Tetratricopeptide repeat (18); SPO22: Meiosis protein SPO22/ZIP4 3 112.4169113781 169117194 like (1e−49); TPR_2: Tetratricopeptide repeat (14);TPR_2: Tetratricopeptide repeat (11); TPR_2: Tetratricopeptide repeat(2.3); GO_MF:GO:0005488, binding# (0.0) 235 Protein IAL1 n = 3 Tax = Zeamays RepID = IAL1_MAIZE (3e−98); DUF260: Protein of unknown functionDUF260 (3.6e−64); GO_MF:GO:0005515, 3 112.5 169217653 169220962 proteinbinding# (7e−44); GO_BP:GO:0007275, TAS#multicellular organismaldevelopment# (3e−98); GO_CC:GO:0005634, nucleus# (3e−98) 236 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = COP2N4_MAIZE(3e−38) 3 112.6 169313187 169317616 237 Retrotransposon protein,putative, LINE subclass n = 1 Tax = Oryza sativa Japonica Group RepID =Q7XET4_ORYSJ (1e−09); GO_MF:GO:0004190, 3 112.6 169348963 169349896penicillopepsin activity# (4e−09); GO_BP:GO:0015074, DNA integration#(4e−09); GO_CC:GO:0005634, nucleus# (4e−09) 238 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = B7ZZD5_MAIZE (2e−56) 3 113.2169538753 169539943 239 Mitogen-activated protein kinase kinase-like n =1 Tax = Oryza sativa Japonica Group RepID = Q5ZE15_ORYSJ (6e−22);GO_MF:GO:0016301, 3 113.2 169539810 169540145 kinase activity# (6e−22);GO_BP:GO:0016301, kinase activity# (6e−22); GO_CC:GO:0005737, cytoplasm#(6e−11) 240 Protein ycf2 n = 2 Tax = Asteraceae RepID = YCF2_GUIAB(2e−32); GO_MF:GO:0017111, nucleoside-triphosphatase activity# (2e−32);3 113.3 169573423 169574052 GO_BP:GO:0032259, methylation# (2e−31);GO_CC:GO:0005739, mitochondrion# (8e−64) 241 DNA binding protein,putative n = 1 Tax = Ricinus communis RepID = B9R9V4_RICCO (1e−26);RNA_pol_Rpc4: RNA polymerase III RPC4 3 113.3 169576810 169579768(4.4e−23); GO_MF:GO:0003899, DNA-directed RNA polymerase III activity#(1e−158); GO_BP:GO:0006383, transcription from RNA polymerase IIIpromoter# (1e−158); GO_CC:GO:0005666, DNA-directed RNA polymerase IIIcomplex# (1e−158) 242 Mitogen-activated protein kinase kinase-like n = 1Tax = Oryza sativa Japonica Group RepID = Q5ZE15_ORYSJ (4e−62); Pkinase:Protein kinase domain 3 113.3 169582103 169583032 (1.8e−25);Pkinase_Tyr: Protein tyrosine kinase (1e−09); GO_MF:GO:0005524, ATPbinding# (3e−62); GO_BP:GO:0006468, protein amino acid phosphorylation#(3e−62); GO_CC:GO:0005886, plasma membrane# (1e−29) 243 Signalrecognition particle subunit srp72, putative n = 1 Tax = Ricinuscommunis RepID = B9RMK3_RICCO (1e−113); GO_MF:GO:0008312, 7S 3 113.45169617037 169619993 RNA binding# (1e−179); GO_BP:GO:0006614,SRP-dependent cotranslational protein targeting to membrane# (1e−179);GO_CC:GO:0048500, signal recognition particle# (1e−179) 244Serine/threonine-protein kinase RIO2 n = 1 Tax = Zea mays RepID =B6SHM1_MAIZE (0.0); Rio2_N: Rio2, N-terminal (9.9e−52); Kdo: 3 113.6169743475 169748165 Lipopolysaccharide kinase (Kdo) (0.0053); APH:Phosphotransferase enzyme family (0.0048); RIO1: RIO1 family (1.6e−72);GO_MF:GO:0016301, kinase activity# (0.0); GO_BP:GO:0016301, kinaseactivity# (0.0); GO_CC:GO:0005737, cytoplasm# (1e−110) 245 MLO-likeprotein 1 n = 1 Tax = Zea mays RepID = Q94CG7_MAIZE (1e−174); Mlo: Mlofamily (3.1e−231); GO_MF:GO:0005516, 3 113.7 169873245 169877270IDA#calmodulin binding# (1e−151); GO_BP:GO:0008219, TAS#cell death#(0.0); GO_CC:GO:0016021, integral to membrane# (0.0) 246 NACdomain-containing protein, putative n = 1 Tax = Ricinus communis RepID =B9S056_RICCO (9e−49); NAM: No apical meristem (NAM) protein (8.6e−35); 3113.7 170022867 170025687 GO_MF:GO:0003677, DNA binding# (1e−130);GO_BP:GO:0045449, regulation of transcription# (1e−130);GO_CC:GO:0005634, nucleus# (2e−27) 247 ATRAD3, putative n = 1 Tax =Ricinus communis RepID = B9S061_RICCO (1e−110); Methyltransf_11:Methyltransferase domain (1.8e−07); 3 113.8 170063542 170064940Methyltransf_12: Methyltransferase domain (3.1e−06); GO_MF:GO:0008168,methyltransferase activity# (1e−107); GO_BP:GO:0008152, metabolicprocess# (1e−107); GO_CC:GO:0005886, plasma membrane# (2e−44) 248 PHDfinger protein n = 4 Tax = Zea mays RepID = B6TYP6_MAIZE (1e−110); C1_3:C1-like domain (0.063); PHD: PHD-finger (1.7e−11); 3 113.9 170106397170110892 GO_MF:GO:0046872, metal ion binding# (1e−110);GO_BP:GO:0046961, proton-transporting ATPase activity, rotationalmechanism# (2e−72); GO_CC:GO:0005634, nucleus# (5e−74) 249 Peptide chainrelease factor, putative n = 1 Tax = Ricinus communis RepID =B9S081_RICCO (8e−54); RF-1: Peptidyl-tRNA hydrolase domain 3 113.9170111448 170114501 (1.9e−06); GO_MF:GO:0003747, translation releasefactor activity# (1e−78); GO_BP:GO:0006415, translational termination#(1e−78); GO_CC:GO:0005737, cytoplasm# (2e−12) 250 Putative2-C-methyl-D-erythritol 4-phosphate cytidyltransferase n = 2 Tax = Oryzasativa RepID = Q5N8G1_ORYSJ (1e−125); IspD: 3 113.9 170115790 170118780Uncharacterized protein family UPF0007 (1.2e−55); NTP_transferase:Nucleotidyl transferase (0.1); GO_MF:GO:0050518, 2-C-methyl-D-erythritol4-phosphate cytidylyltransferase activity# (1e−125); GO_BP:GO:0016114terpenoid biosynthetic process# (1e−125); GO_CC:GO:0009570,IDA#chloroplast stroma# (5e−91) 251 Zgc:162613 protein n = 3 Tax = Daniorerio RepID = A3KNW8_DANRE (3e−30); DUF647: Protein of unknown function,DUF647 3 113.9 170119830 170125020 (9.6e−153); GO_MF:GO:0003674,ND#molecular function# (3e−26); GO_BP:GO:0010224, response to UV-B#(2e−29); GO_CC:GO:0016021, integral to membrane# (6e−30) 252 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C0HGN3_MAIZE(1e−23) 3 113.9 170144285 170144599 253 Putative uncharacterized proteinn = 1 Tax = Zea mays RepID = Q6JAD6_MAIZE (3e−09) 3 114 170148931170218903 254 AP2 domain containing protein n = 1 Tax = Zea mays RepID =B6UDE8_MAIZE (1e−103); AP2: AP2 domain (2.5e−20); 3 114.1 170221982170223800 GO_MF:GO:0003700, transcription factor activity# (1e−103);GO_BP:GO:0045449, regulation of transcription# (1e−103);GO_CC:GO:0005634, nucleus# (1e−103) 255 Putative uncharacterized proteinSb03g042100 n = 2 Tax = Andropogoneae RepID = C5XEN6_SORBI (1e−26); 3114.1 170248244 170251095 GO_MF:GO:0051082, unfolded protein binding#(3e−20); GO_BP:GO:0006457, protein folding# (3e−20) 256 Nucleic acidbinding protein, putative n = 1 Tax = Ricinus communis RepID =B9SKB5_RICCO (9e−97); 3 114.1 170277042 170281693 GO_MF:GO:0003723, RNAbinding# (1e−132); GO_BP:GO:0030154, cell differentiation# (4e−30);GO_CC:GO:0005634, nucleus# (7e−81) 257 M25 protein (Fragment) n = 2 Tax= Zea mays RepID = Q84V73_MAIZE (1e−112); SRF-TF: SRF-type 3 114.3170365225 170381998 transcription factor (DNA-binding and dimerisationdomain) (6.2e−31); K-box: K-box region (3.6e−42); GO_MF:GO:0043565,sequence-specific DNA binding# (1e−112); GO_BP:GO:0045449, regulation oftranscription# (1e−112); GO_CC:GO:0005634, nucleus# (1e−112) 258Pentatricopeptide repeat protein PPR986-12 n = 1 Tax = Zea mays RepID =B6SPB1_MAIZE (7e−29); 3 114.3 170382014 170383688 PPR: PPR repeat(0.01); GO_MF:GO:0004553, hydrolase activity, hydrolyzing O-glycosylcompounds# (2e−43); GO_BP:GO:0005975, carbohydrate metabolic process#(2e−43); GO_CC:GO:0009507, chloroplast# (2e−15) 259 Transcriptionalfactor TINY n = 1 Tax = Zea mays RepID = B6SP65_MAIZE (2e−67); AP2: AP2domain (5.6e−17); 3 114.4 170423568 170424380 GO_MF:GO:0003700,transcription factor activity# (2e−67); GO_BP:GO:0045449, regulation oftranscription# (2e−67); GO_CC:GO:0005634, nucleus# (2e−67) 260Leucoanthocyanidin reductase n = 1 Tax = Zea mays RepID = B6T842_MAIZE(4e−65); GO_MF:GO:0050662, 3 114.4 170426281 170428946 coenzyme binding#(4e−65); GO_BP:GO:0044237, cellular metabolic process# (4e−65);GO_CC:GO:0005694, chromosome# (2e−40) 261 Phosphoinositide5-phosphatase, putative n = 1 Tax = Ricinus communis RepID =B9RR38_RICCO (2e−52) 3 114.4 170431126 170433790 262 Hydrolase-likeprotein n = 2 Tax = Oryza sativa RepID = Q5N8H1_ORYSJ (1e−164); PGAP1:PGAP1-like protein (0.016); 3 114.4 170440239 170445382 Thioesterase:Thioesterase domain (0.0067); Abhydrolase_1: alpha/beta hydrolase fold(1.3e−07); GO_MF:GO:0016788, hydrolase activity, acting on ester bonds#(1e−164); GO_BP:GO:0009058, biosynthetic process# (1e−164);GO_CC:GO:0005739, mitochondrion# (8e−42) 263 Cytochrome c n = 5 Tax =Magnoliophyta Rep ID = CYC_ORYSJ (4e−58); Cytochrom_C: Cytochrome c(8.1e−34); 3 114.5 170498763 170503087 GO_MF:GO:0046872, metal ionbinding# (4e−58); GO_BP:GO:0022900, electron transport chain# (4e−58);GO_CC:GO:0070469, respiratory chain# (4e−58) 264 DNA glycosylase n = 1Tax = Micromonas pusilla CCMP1545 RepID = C1MR64_9CHLO (2e−47);SNARE_assoc: SNARE associated Golgi protein (1.5e−48); 3 114.5 170535675170538882 GO_MF:GO:0005524, ATP binding# (3e−19); GO_BP:GO:0006915,apoptosis# (3e−19); GO_CC:GO:0009507, chloroplast# (2e−77) 265Pentatricopeptide (PPR) repeat-containing protein-like protein n = 2 Tax= Oryza sativa Japonica Group RepID = Q6ZIP5_ORYSJ (1e−101); TPR_4: 3114.5 170538931 170540493 Tetratricopeptide repeat (12); PPR: PPR repeat(2e−08); PPR: PPR repeat (1.5e−06); PPR: PPR repeat (6.5e−09); TPR_4:Tetratricopeptide repeat (1.4); PPR: PPR repeat (2.5); PPR: PPR repeat(2.6); GO_MF:GO:0005488, binding# (1e−141); GO_CC:GO:0005739,mitochondrion# (1e−102) 266 Putative uncharacterized protein n = 1 Tax =Zea mays RepID = B6SSE8_MAIZE (7e−82) 3 114.5 170545652 170548181 267Transposon protein Pong sub-class n = 1 Tax = Zea mays RepID =B6TCG7_MAIZE (1e−29); Plant_tran: Plant transposon protein (1.1e−06); 3114.6 170678312 170678644 GO_CC:GO:0005840, ribosome# (2e−28) 268Putative polyprotein n = 1 Tax = Zea mays RepID = Q8SA93_MAIZE (1e−118);GO_MF:GO:0003964, RNA-directed DNA polymerase, group II 3 114.6170703404 170704531 intron encoded# (1e−118); GO_BP:GO:0015074, DNAintegration# (1e−118); GO_CC:GO:0005634, nucleus# (1e−118) 269 Proteinbinding protein, putative n = 1 Tax = Ricinus communis RepID =B9SS21_RICCO (5e−27); SWIB: SWIB/MDM2 domain (0.0017); Plus-3: 3 114.6170707230 170710209 Plus-3 domain (2.3e−24); GO_MF:GO:0046872, metal ionbinding# (7e−84); GO_BP:GO:0016570, histone modification# (7e−84);GO_CC:GO:0005634, nucleus# (7e−84) 270 Putative uncharacterized proteinSb03g041940 n = 1 Tax = Sorghum bicolor RepID = C5XEM1_SORBI (1e−44);GYF: GYF domain (1.2e−05); 3 114.6 170732741 170733199 GO_MF:GO:0046872,metal ion binding# (6e−11); GO_BP:GO:0016570, histone modification#(6e−11); GO_CC:GO:0005634, nucleus# (6e−11) 271 Ubiquitin-proteinligase, putative n = 1 Tax = Ricinus communis RepID = B9SG36_RICCO(1e−157); U-box: U-box domain (9.8e−24); 3 114.7 170771543 170775469Arm: Armadillo/beta-catenin-like repeat (4.9e−09); Arm:Armadillo/beta-catenin-like repeat (8e−05); Arm:Armadillo/beta-catenin-like repeat (1.9e−09); Arm:Armadillo/beta-catenin-like repeat (0.003); Arm:Armadillo/beta-catenin-like repeat (0.01); Arm:Armadillo/beta-catenin-like repeat (0.66); GO_MF:GO:0005488, binding#(0.0); GO_BP:GO:0016567, IGI#protein ubiquitination# (0.0);GO_CC:GO:0000151, ubiquitin ligase complex# (0.0) 272 NACdomain-containing protein 48 n = 1 Tax = Zea mays RepID = B6TI55_MAIZE(1e−153); NAM: No apical meristem (NAM) protein 3 114.7 170820468170822609 (1.9e−83); GO_MF:GO:0003677, DNA binding# (1e−151);GO_BP:GO:0045449, regulation of transcription# (1e−151);GO_CC:GO:0005634, nucleus# (1e−130) 273 Ankyrin-like protein n = 1 Tax =Zea mays RepID = B6U1Y2_MAIZE (2e−35); GO_MF:GO:006740, transferaseactivity# (7e−32); 3 114.8 170867201 170867947 GO_BP:GO:0055114,oxidation reduction# (9e−23); GO_CC:GO:0005794, IDA#Golgi apparatus#(5e−36) 274 Gibberellin 20 oxidase 2 n = 16 Tax = Oryza RepID =GAOX2_ORYSJ (1e−162); 2OG-FeII_Oxy: 2OG-Fe(II) oxygenase superfamily(1.4e−48); 3 114.8 170899605 170902451 GO_MF:GO:0046872, metal ionbinding# (1e−162); GO_BP:GO:0055114, oxidation reduction# (1e−162);GO_CC:GO:0005737, cytoplasm# (1e−103) 275 Retrotransposon protein,putative, unclassified n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QSH2_ORYSJ (5e−13); GO_MF:GO:0004523, 3 114.8 170908908 170925761ribonuclease H activity# (5e−13); GO_BP:GO:0006278, RNA-dependent DNAreplication# (5e−13) 276 B1248C03.3 protein n = 1 Tax = Oryza sativaJaponica Group RepID = Q7FAL2_ORYSJ (1e−63); GO_MF:GO:0003964,RNA-directed DNA polymerase, 3 114.8 170909162 170909755 group II intronencoded# (1e−63); GO_BP:GO:0006278, RNA-dependent DNA replication#(1e−63) 277 Exosome complex exonuclease rrp45, putative n = 1 Tax =Ricinus communis RepID = B9SYQ9_RICCO (2e−49); RNase_PH_C: 3 114.8170912362 170916073 3′ exoribonuclease family, domain 2 (1.5e−09);GO_MF:GO:0003723, RNA binding# (2e−67); GO_BP:GO:0006396, RNAprocessing# (2e−67); GO_CC:GO:0005737, cytoplasm# (3e−48) 278 PHD fingerprotein-like n = 2 Tax = Oryza sativa RepID = Q5N7H9_ORYSJ (4e−23); PHD:PHD-finger (2.3e−05); zf-C3HC4: Zinc finger, 3 114.9 171032370 171032891C3HC4 type (RING finger) (0.014); PHD: PHD-finger (3.1e−07);GO_MF:GO:0046872, metal ion binding# (9e−28); GO_CC:GO:0016021, integralto membrane# (1e−20) 279 Unknow protein n = 1 Tax = Oryza sativaJaponica Group RepID = Q60EP7_ORYSJ (1e−40) 3 114.9 171147903 171155837280 Unknow protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q60EP7_ORYSJ (3e−29) 3 114.9 171149713 171151352 281 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = 048967_MAIZE(2e−17) 3 114.9 171379246 171381120 282 MADS-box transcription factor 2n = 5 Tax = Poaceae RepID = MADS2_ORYSJ (1.e−110); SRF-TF: SRF-typetranscription factor (DNA-binding 3 114.9 171427699 171430664 anddimerisation domain) (9.6e−31); K-box: K-box region (1.5e−28);GO_MF:GO:0043565, sequence-specific DNA binding# (1.e−110);GO_BP:GO:0045449, regulation of transcription# (1.e−110);GO_CC:GO:0005634, nucleus# (1e−110) 283 Amino acid carrier n = 2 Tax =Andropogoneae RepID = B6T9X6_MAIZE (0.0); Aa_trans: Transmembrane aminoacid transporter protein 3 114.9 171435067 171438501 (1.4e−146);Phage_holin_3: Phage holin family (Lysis protein S) (0.063);GO_MF:GO:0015293, symporter activity# (0.0); GO_BP:GO:0015804, neutralamino acid transport# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 284 Putative dual-specific kinase DSK1 n = 1 Tax = Oryza sativaJaponica Group RepID = Q5N7J0_ORYSJ (0.0); Pkinase_Tyr: Protein 3 114.9171639409 171646343 tyrosine kinase (7.5e−18); Pkinase: Protein kinasedomain (1.5e−26); GO_MF:GO:0005524, ATP binding# (0.0);GO_BP:GO:0006468, protein amino acid phosphorylation# (0.0);GO_CC:GO:0005886, plasma membrane# (4e−85) 285 F-box/LRR-repeat protein2 n = 2 Tax = Andropogoneae RepID = B4G0B1_MAIZE (0.0); LRR_1: LeucineRich Repeat (71); 3 114.9 171709202 171713046 LRR_1: Leucine Rich Repeat(17); LRR_1: Leucine Rich Repeat (8.1); LRR_1: Leucine Rich Repeat (94);LRR_1: Leucine Rich Repeat (1e+02); GO_MF:GO:0005515, protein binding#(1e−158); GO_BP:GO:0051603, proteolysis involved in cellular proteincatabolic process# (1e−106); GO_CC:GO:0000151, ubiquitin ligase complex#(2e−13) 286 DNA ligase-like n = 1 Tax = Oryza sativa Japonica GroupRepID = Q8RZQ7_ORYSJ (0.0); DRMBL: DNA repair metallo-beta-lactamase 3114.9 171714003 171716849 (7.1e−11); GO_MF:GO:0016874, ligase activity#(0.0); GO_BP:GO:0006468, protein amino acid phosphorylation# (1e−105);GO_CC:GO:0005634, nucleus# (4e−20) 287 NADH dehydrogenase I subunit N n= 2 Tax = Andropogoneae RepID = B4FKX8_MAIZE (7e−93); GO_MF:GO:0016655,3 115 171441464 171443435 oxidoreductase activity, acting on NADH orNADPH, quinone or similar compound as acceptor# (1e−121);GO_BP:GO:0055114, oxidation reduction# (1e−121); GO_CC:GO:0016020,membrane# (1e−121) 288 Non-imprinted in Prader-Willi/Angelman syndromeregion protein, putative n = 1 Tax = Ricinus communis RepID =B9SSN6_RICCO 3 115 171443315 171448732 (1e−134); DUF6: Integral membraneprotein DUF6 (0.055); DUF803: Protein of unknown function (DUF803)(6e−208); GO_CC:GO:0005886, plasma membrane# (2e−90) 289 Putativeleucine-rich repeat receptor-like kinase n = 1 Tax = Oryza sativa IndicaGroup RepID = Q66QA8_ORYSI (2e−84); 3 115 171466052 171469839 LRR_1:Leucine Rich Repeat (2.1); LRR_1: Leucine Rich Repeat (0.23); LRR_1:Leucine Rich Repeat (0.026); LRR_1: Leucine Rich Repeat (4.5); LRR_1:Leucine Rich Repeat (9.6); LRR_1: Leucine Rich Repeat (26); LRR_1:Leucine Rich Repeat (0.14); LRR_1: Leucine Rich Repeat (1.2e+02);GO_MF:GO:0005524, ATP binding# (1e−86); GO_BP:GO:0006468, protein aminoacid phosphorylation# (1e−86) 290 Seven-transmembrane-domain protein 1 n= 2 Tax = Zea mays RepID = B6SU55_MAIZE (7e−72); MtN3_slv: MtN3/sativafamily (3.5e−22); MtN3_slv: MtN3/sativa 3 115 171748802 171752467 family(2.3e−15); GO_MF:GO:0008270, zinc ion binding# (7e−39);GO_BP:GO:0010208, IMP#pollen wall assembly# (6e−37); GO_CC:GO:0016021,integral to membrane# (1.e−105) 291 CDPK-related protein kinase n = 5Tax = Poaceae RepID = B6SHU9_MAIZE (1e−55); GO_MF:GO:0005524, ATPbinding# (3e−57); GO_BP:GO:006301, 3 115 171777495 171778056 kinaseactivity# (1e−55); GO_CC:GO:0016020, membrane# (2e−39) 292Pentatricopeptide repeat protein PPR868-14 n = 2 Tax = AndropogoneaeRepID = B6U7N3_MAIZE (0.0); PPR: PPR repeat (0.00083); TPR_4: 3 115.1171779387 171781432 Tetratricopeptide repeat (6.3); TPR_4:Tetratricopeptide repeat (5.4); PPR: PPR repeat (1.2e−05); TPR_4:Tetratricopeptide repeat (1.5); PPR: PPR repeat (1.2e−08); PPR: PPRrepeat (0.79); PPR: PPR repeat (2.5e−10); PPR: PPR repeat (0.18); TPR_4:Tetratricopeptide repeat (41); PPR: PPR repeat (0.057); PPR: PPR repeat(5.2); GO_MF:GO:0005488, binding# (4e−91); GO_CC:GO:0009536, plastid#(8e−89) 293 Importin beta-1, putative n = 1 Tax = Ricinus communis RepID= B9SKY7_RICCO (2e−13); GO_MF:GO:0008565, protein transporter activity#(8e−17); 3 115.1 171815263 171816887 GO_BP:GO:0008565, proteintransporter activity# (8e−17); GO_CC:GO:0009507, chloroplast# (6e−15)294 Phytochrome B n = 8 Tax = Sorghum RepID = PHYB SORBI (2e−49);GO_MF:GO:0042803, protein homodimerization activity# (2e−49); 3 115.15171817584 171822908 GO_BP:GO:0050896, response to stimulus# (2e−49);GO_CC:GO:0016020, membrane# (2e−49) 295 Pectinesterase n = 2 Tax = Oryzasativa RepID = Q8LJK2_ORYSJ (0.0); Pectinesterase: Pectinesterase(3e−143); GO_MF:GO:0045330, 3 115.3 171894912 171898201 aspartylesterase activity# (0.0); GO_BP:GO:0042545, cell wall modification#(0.0); GO_CC:GO:0005618, IDA#cell wall# (0.0) 296 Nitroreductase familyprotein, putative n = 1 Tax = Oryza sativa Japonica Group RepID =Q8LMV1_ORYSJ (0.0); GO_MF:GO:0016491, oxidoreductase 3 115.3 171898843171903374 activity# (0.0) 297 Glycogenin-like protein n = 4 Tax = Oryzasativa RepID = Q5NA53_ORYSJ (0.0); Glyco_transf_8: Glycosyl transferasefamily 8 (2.7e−61); GO_MF:GO:0016757, 3 115.7 172050750 172055326transferase activity, transferring glycosyl groups# (0.0) 298OSJNBa0095H06.12 protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q7XS07_ORYSJ (3e−51); GO_MF:GO:0004386, helicase activity# (8e−47) 3115.9 172179901 172180535 299 Putative retrotransposon protein n = 1 Tax= Phyllostachys edulis RepID = D3IVP0_9POAL (1e−123); GO_MF:GO:0004386,helicase activity# (1e−115) 3 115.9 172188829 172189985 300 AT hookmotif-containing protein, putative n = 2 Tax = Oryza sativa JaponicaGroup RepID = Q2R0Z1_ORYSJ (0.0); DUF889: Eukaryotic protein of unknown3 115.9 172189989 172196219 function (DUF889) (6.4e−84);GO_MF:GO:0004386, helicase activity# (0.0) 301 Putative uncharacterizedprotein Sb03g041650 n = 1 Tax = Sorghum bicolor RepID = C5XRE3_SORBI(4e−52); DUF506: Protein of unknown function (DUF506) 3 115.9 172198615172200295 (3.7e−36); GO_MF:GO:0005515, protein binding# (8e−20);GO_CC:GO:0005634, nucleus# (8e−20) 302 Beta-glucanase-like protein n = 1Tax = Oryza sativa Japonica Group RepID = Q5N9F8_ORYSJ (0.0);Glyco_hydro_43: Glycosyl hydrolases family 43 (0.002); 3 115.9 172257039172260656 GO_MF:GO:0004553, hydrolase activity, hydrolyzing O-glycosylcompounds# (0.0); GO_BP:GO:0005975, carbohydrate metabolic process#(0.0); GO_CC:GO:0030529, ribonucleoprotein complex# (0.0) 303 Putativeuncharacterized protein Sb07g027290 n = 1 Tax = Sorghum bicolor RepID =C5YTM6_SORBI (9e−16) 3 116 172150745 172150990 304 4,5-DOPA dioxygenaseextradiol n = 2 Tax = Zea mays RepID = B4FQS1_MAIZE (1e−142); LigB:Catalytic LigB subunit of aromatic ring-opening 3 116 172333476172336038 dioxygenase (5e−96); GO_MF:GO:0016702, oxidoreductaseactivity, acting on single donors with incorporation of molecularoxygen, incorporation of two atoms of oxygen# (1e−142);GO_BP:GO:0055114, oxidation reduction# (1e−142); GO_CC:GO:0005737,cytoplasm# (2e−72) 305 Catalytic/protein phosphatase type 2C n = 2 Tax =Zea mays RepID = B6TWB0_MAIZE (6e−21); GO_MF:GO:0003824, catalyticactivity# (6e−21); 3 116 172348756 172349196 GO_BP:GO:0004721,phosphoprotein phosphatase activity# (2e−17); GO_CC:GO:0005886, plasmamembrane# (2e−11) 306 Leucine-rich receptor protein kinase-like proteinn = 1 Tax = Oryza sativa Japonica Group RepID = Q5N9G1_ORYSJ (1e−149);LRR_1: Leucine Rich Repeat (5.5); 3 116 172374729 172376996 LRR_1:Leucine Rich Repeat (0.11); LRR_1: Leucine Rich Repeat (13); Pkinase:Protein kinase domain (3.8e−40); Pkinase_Tyr: Protein tyrosine kinase(6.1e−18); LRR_1: Leucine Rich Repeat (2.3e+02); GO_MF:GO:0016301,kinase activity# (0.0); GO_BP:GO:0016301, kinase activity# (0.0);GO_CC:GO:0016021, integral to membrane# (1e−120) 307 Putativeuncharacterized protein n = 2 Tax = Zea mays RepID = B6TK95_MAIZE(1e−52) 3 116.1 172441684 172443173 308 Beta-1,3-galactosyltransferasesqv-2 n = 1 Tax = Zea mays RepID = B6TIX7_MAIZE (1e−47);GO_MF:GO:0016757, transferase activity, transferring glycosyl 3 116.1172444363 172445137 groups# (1e−47); GO_BP:GO:0006486, protein aminoacid glycosylation# (1e−47); GO_CC:GO:0016021, integral to membrane#(1e−47) 309 Beta-1,3-galactosyltransferase sqv-2 n = 1 Tax = Zea maysRepID = B6TIX7_MAIZE (3e−26); GO_MF:GO:0016757, transferase activity,transferring glycosyl 3 116.1 172445592 172446433 groups# (3e−26);GO_BP:GO:0006486, protein amino acid glycosylation# (3e−26);GO_CC:GO:0016021, integral to membrane# (3e−26) 310 Mitotic checkpointprotein n = 1 Tax = Zea mays RepID = B6U4F6_MAIZE (0.0); MAD: Mitoticcheckpoint protein (3.1e−128); Pox_A_type_inc: 3 116.15 172447355172477549 Viral A-type inclusion protein repeat (20); Pox_A_type_inc:Viral A-type inclusion protein repeat (69); Pox_A_type_inc: Viral A-typeinclusion protein repeat (14); Pox_A_type_inc: Viral A-type inclusionprotein repeat (5.7); Pox_A_type_inc: Viral A-type inclusion proteinrepeat (19); GO_MF:GO:0005515, protein binding# (1e−16);GO_BP:GO:0051301, cell division# (1e−16); GO_CC:GO:0005856,cytoskeleton# (1e−16) 311 Putative uncharacterized protein n = 1 Tax =Zea mays RepID = C0PES4_MAIZE (1e−130) 3 116.3 172522488 172546848 312SnRK1-interacting protein 1 n = 2 Tax = Zea mays RepID = B6TIS1_MAIZE(1e−103); GO_MF:GO:0050897, IDA#cobalt ion binding# (2e−53); 3 116.35172566013 172574173 GO_CC:GO:0009507, chloroplast# (7e−31) 313 PutativeRRM-containing protein SEB-4 n = 2 Tax = Oryza sativa RepID =Q5N8W4_ORYSJ (1e−139); RRM_1: RNA recognition motif. 3 116.5 172624870172629189 (a.k.a. RRM, RB (2.1e−17); GO_MF:GO:0003676, nucleic acidbinding# (1e−139) 314 Galactosyltransferase-like protein n = 1 Tax =Oryza sativa Japonica Group RepID = Q5N8W6_ORYSJ (8e−54); RRM_1: RNArecognition motif. 3 116.5 172675337 172678828 (a.k.a. RRM, RB(9.2e−16); GO_MF:GO:0003676, nucleic acid binding# (4e−55) 315 SUN4 n =1 Tax = Zea mays RepID = D3KCC3_MAIZE (0.0); F5_F8_type_C: F5/8 type Cdomain (0.068); Sad1_UNC: Sad1/UNC-like C-terminal (5.9e−59); 3 116.5172806294 172809079 GO_CC:GO:0016021, integral to membrane# (2e−32) 316ATP binding protein n = 1 Tax = Zea mays RepID = B6U192_MAIZE (1e−147);Kinesin: Kinesin motor domain (3.7e−37); Nodulin-like: Nodulin-like(2.5e−09); 3 116.5 172813626 172817058 GO_MF:GO:0005524, ATP binding#(1e−147); GO_BP:GO:0007018, microtubule-based movement# (1e−147);GO_CC:GO:0005874, microtubule# (1e−147) 317 Putative auxin-regulatedprotein n = 1 Tax = Oryza sativa Japonica Group RepID = Q6YYY6_ORYSJ(6e−14); DUF966: Domain of unknown function (DUF966) 3 116.5 172822909172824557 (5.9e−06) 318 Serine endopeptidase degp2, putative n = 1 Tax =Ricinus communis RepID = B9S3X1_RICCO (2e−09); GO_MF:GO:0032440,2-alkenal reductase activity# (2e−09); 3 116.5 172826631 172828767GO_BP:GO:0055114, oxidation reduction# (2e−09); GO_CC:GO:0016020,membrane# (8e−09) 319 Pyrimidine-specific ribonucleoside hydrolase rihAn = 4 Tax = Andropogoneae RepID = B6T5H3_MAIZE (4e−34); GO_MF:GO:006787,hydrolase activity# (4e−34); 3 116.5 172847460 172847835GO_BP:GO:0008152, metabolic process# (2e−20); GO_CC:GO:0005829,IDA#cytosol# (1e−15) 320 ATP binding protein n = 4 Tax = Zea mays RepID= B6U8U2_MAIZE (0.0); GO_MF:GO:0005524, ATP binding# (0.0);GO_BP:GO:0006468, protein amino acid 3 116.5 172890112 172896927phosphorylation# (0.0) 321 Putative uncharacterized protein Sb02g036510n = 1 Tax = Sorghum bicolor RepID = C5XBL8_SORBI (3e−15) 3 116.5172900980 172902970 322 Putative uncharacterized protein Sb03g032780 n =1 Tax = Sorghum bicolor RepID = C5XI97_SORBI (1e−115); GO_BP:GO:006998,cell wall macromolecule 3 116.5 172903355 172904665 catabolic process#(9e−23) 323 F-box protein interaction domain containing protein n = 2Tax = Zea mays RepID = B6TT97_MAIZE (1e−163); F-box: F-box domain(0.00082); 3 116.5 172905270 172906947 GO_MF:GO:0016301, kinaseactivity# (4e−20); GO_BP:GO:0016301, kinase activity# (4e−20) 324Putative chloride channel n = 2 Tax = Oryza sativa RepID = Q5N8W8_ORYSJ(0.0); Voltage_CLC: Voltage gated chloride channel (6.3e−82); 3 116.8172852406 172855470 DUF2062: Uncharacterized protein conserved in(0.084); TAT_signal: TAT (twin-arginine translocation) pat (0.092); CBS:CBS domain (1.1e−08); GO_MF:GO:0005247, voltage-gated chloride channelactivity# (0.0); GO_BP:GO:0055085, transmembrane transport# (0.0);GO_CC:GO:0016021, integral to membrane# (0.0) 325 P0648C09.24 protein n= 1 Tax = Oryza sativa Japonica Group RepID = Q8RYX6_ORYSJ (1e−147);DUF266: Arabidopsis protein of unknown function, 3 116.8 173002202173004319 DUF266 (3.9e−185); GO_MF:GO:0008375,acetylglucosaminyltransferase activity# (0.0); GO_CC:GO:0016020,membrane# (0.0) 326 DnaJ protein n = 2 Tax = Zea mays RepID =B6T720_MAIZE (1e−144); DnaJ: DnaJ domain (4.2e−18); DnaJ_C: DnaJ Cterminal region (6.8e−10); 3 116.8 173004861 173007364 GO_MF:GO:0051082,unfolded protein binding# (1e−162); GO_BP:GO:0006457, protein folding#(1e−162) 327 Beta-galactosidase n = 2 Tax = Andropogoneae RepID =B6U0W2_MAIZE (0.0); Glyco_hydro_35: Glycosyl hydrolases family 35(2.8e−170); 3 116.8 173015374 173022743 Glyco_hydro_2_N: Glycosylhydrolases family 2, sugar binding domain (0.08); Gal_Lectin: Galactosebinding lectin domain (0.00035); GO_MF:GO:0043169, cation binding#(0.0); GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0048046,IDA#apoplast# (0.0) 328 Signal recognition particle receptor alphasubunit, putative n = 1 Tax = Ricinus communis RepID = B9STS5_RICCO(1e−54); SRP54: SRP54-type protein, GTPase 3 117.1 173134957 173135803domain (5.7e−05); GO_MF:GO:0017111, nucleoside-triphosphatase activity#(2e−58); GO_BP:GO:0006614, SRP-dependent cotranslational proteintargeting to membrane# (2e−58); GO_CC:GO:0005783, IDA#endoplasmicreticulum# (2e−53) 329 Beta-galactosidase n = 1 Tax = Oryza sativaIndica Group RepID = B2Z6M9_ORYSI (0.0); Glyco_hydro_35: Glycosylhydrolases family 35 (2.2e−162); 3 117.1 173201293 173208931Glyco_hydro_42: Beta- galactosidase (0.025); Glyco_hydro_2_N: Glycosylhydrolases family 2, sugar binding domain (0.043); Gal_Lectin: Galactosebinding lectin domain (1.1e−24); GO_MF:GO:0043169, cation binding#(0.0); GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0048046,IDA#apoplast# (0.0) 330 Src2-like protein n = 1 Tax = Zea mays RepID =B6TF82_MAIZE (9e−24); XYPPX: XYPPX repeat (1e+02); XYPPX: XYPPX repeat(1.7e+02); XYPPX: XYPPX 3 117.3 173284946 173285813 repeat (1e+02);XYPPX: XYPPX repeat (1.2e+02); XYPPX: XYPPX repeat (1e+02); XYPPX: XYPPXrepeat (25); XYPPX: XYPPX repeat (1.2e+02) 331 OSJNBa0009P12.18 proteinn = 3 Tax = Oryza sativa RepID = Q5VSV8_ORYSA (1e−34); GO_MF:GO:0017076,purine nucleotide binding# (1e−34); 3 117.3 173287877 173288635GO_BP:GO:0006094, gluconeogenesis# (1e−34); GO_CC:GO:0032040,small-subunit processome# (5e−22) 332 Putative uncharacterized protein n= 1 Tax = Zea mays RepID = C0PKR7_MAIZE (9e−69) 3 117.5 173371885173373794 333 1-phosphatidylinositol-3-phosphate 5-kinase FAB1-likeprotein n = 1 Tax = Oryza sativa Japonica Group RepID = Q6H5I5_ORYSJ(1e−122); GO_MF:GO:0016307, 3 117.5 173396687 173400469phosphatidylinositol phosphate kinase activity# (1e−161);GO_BP:GO:0046488, phosphatidylinositol metabolic process# (1e−161);GO_CC:GO:0005739, mitochondrion# (1e−37) 334 DNA polymerase I n = 4 Tax= Andropogoneae RepID = B6U7X8_MAIZE (1e−166); 5_3_exonuc_N: 5′-3′exonuclease, N-terminal resolvase-like domain 3 117.5 173468914173471177 (1.1e−12); 5_3_exonuc: 5′-3′ exonuclease, C-terminal SAM fold(4.2e−06); GO_MF:GO:0008409, 5′-3′ exonuclease activity# (1e−166);GO_BP:GO:0006281, DNA repair# (8e−20); GO_CC:GO:0005622, intracellular#(8e−20) 335 HAT family dimerisation domain containing protein n = 1 Tax= Oryza sativa Japonica Group RepID = Q2QRD1_ORYSJ (1e−78); zf-BED: BEDzinc finger 3 117.8 173511054 173512633 (4.2e−10); hATC: hAT familydimerisation domain (4.1e−25); GO_MF:GO:0046983, protein dimerizationactivity# (1e−78) 336 Myb transcription factor n = 3 Tax = Oryza sativaRepID = Q5TKI8_ORYSJ (3e−84); Myb_DNA-binding: Myb-like DNA-bindingdomain (1.1e−10); 3 117.9 173520319 173521525 Myb_DNA-binding: Myb-likeDNA-binding domain (6.9e−11); GO_MF:GO:0003677, DNA binding# (1e−118);GO_BP:GO:0045449, regulation of transcription# (1e−118);GO_CC:GO:0005634, nucleus# (1e−118) 337 ATP binding protein n = 1 Tax =Zea mays RepID = B6U6Y9_MAIZE (0.0); dNK: Deoxynucleoside kinase(3.7e−29); GO_MF:GO:0016773, phosphotransferase 3 118.3 173585707173590684 activity, alcohol group as acceptor# (0.0); GO_BP:GO:0006139,nucleobase, nucleoside, nucleotide and nucleic acid metabolic process#(0.0); GO_CC:GO:0005634, nucleus# (1e−171) 338 HAT family dimerisationdomain containing protein n = 1 Tax = Oryza sativa Japonica Group RepID= Q2QRD1_ORYSJ (1e−16); zf-BED: BED zinc finger (0.0026); 3 118.3173654679 173655715 GO_MF:GO:0046983, protein dimerization activity#(1e−16) 339 Putative uncharacterized protein Sb01g029380 n = 1 Tax =Sorghum bicolor RepID = C5WSG6_SORBI (2e−27); GO_MF:GO:0046983, proteindimerization 3 118.3 173681345 173682358 activity# (3e−10);GO_CC:GO:0016021, integral to membrane# (4e−10) 340 OSJNBa0088K19.7protein n = 3 Tax = Oryza sativa RepID = Q7XUZ4_ORYSJ (1e−116); DUF668:Protein of unknown function (DUF668) (4e−54); 3 118.3 173688595173699327 GO_MF:GO:0016301, kinase activity# (1e−119); GO_BP:GO:0016301,kinase activity# (1e−119); GO_CC:GO:0005886, plasma membrane# (1e−162)341 Erythroid differentiation-related factor 1-like protein n = 2 Tax =Oryza sativa RepID = Q5N730_ORYSJ (0.0); GO_MF:GO:0005515, proteinbinding# (1e−25); 3 118.3 173735061 173741621 GO_BP:GO:0045449,regulation of transcription# (4e−35); GO_CC:GO:0005886, plasma membrane#(0.0) 342 Amidophosphoribosyltransferase n = 2 Tax = Andropogoneae RepID= B6SRU6_MAIZE (0.0); GATase_2: Glutamine amidotransferases class-II(3.4e−35); 3 118.3 173801435 173803391 Pribosyltran: Phosphoribosyltransferase domain (2.8e−15); GO_MF:GO:0016757, transferase activity,transferring glycosyl groups# (0.0); GO_BP:GO:0009116, nucleosidemetabolic process# (0.0); GO_CC:GO:0005618, IDA#cell wall# (1e−166) 3433-phosphoinositide-dependent protein kinase 1 n = 4 Tax = AndropogoneaeRepID = B6UBV8_MAIZE (0.0); Pkinase_Tyr: Protein tyrosine kinase(2.5e−10); 3 118.4 174059840 174064980 Pkinase: Protein kinase domain(3.3e−83); GO_MF:GO:0016301, kinase activity# (0.0); GO_BP:GO:0016301,kinase activity# (0.0); GO_CC:GO:0016023, cytoplasmic membrane-boundedvesicle# (2e−83) 344 SKP1-like protein 1A n = 1 Tax = Zea mays RepID =B6UDE5_MAIZE (4e−64); Skp1_POZ: Skp1 family, tetramerisation domain(3.6e−23); Skp1: Skp1 family, 3 118.4 174067213 174067846 dimerisationdomain (2.9e−38); GO_MF:GO:0005515, protein binding# (4e−64);GO_BP:GO:0006511, ubiquitin-dependent protein catabolic process#(4e−64); GO_CC:GO:0005634, nucleus# (2e−42) 345 Malic enzyme (Fragment)n = 1 Tax = Oryza sativa Japonica Group RepID = Q53RP5_ORYSJ (1e−140);FAR1: FAR1 family (2.9e−27); MULE: MULE transposase 3 118.4 174149824174151436 domain (2.5e−19); GO_MF:GO:0051287, NAD or NADH binding#(1e−140); GO_BP:GO:0055114, oxidation reduction# (1e−140);GO_CC:GO:0005622, intracellular# (1e−137) 346 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = C4JA86_MAIZE (2e−11) 3 118.4174214742 174215602 347 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = C0PNM6_MAIZE (1e−35) 3 118.4 174215246 174250947 348Putative cytochrome P450 monooxygenase n = 2 Tax = Oryza sativa RepID =Q6K6C8_ORYSJ (2e−39); GO_MF:GO:0046872, metal ion binding# (1e−52); 3118.4 174269792 174270437 GO_BP:GO:0055114, oxidation reduction# (1e−52)349 POT family protein n = 1 Tax = Zea mays RepID = B6ST62_MAIZE (0.0);MFS_1: Major Facilitator Superfamily (0.035); PTR2: POT family(3.1e−47); 3 118.4 174335482 174341351 GO_MF:GO:0005215, transporteractivity# (0.0); GO_BP:GO:0006857, oligopeptide transport# (0.0);GO_CC:GO:0016020, membrane# (0.0) 350 Peptide transporter PTR2-B n = 2Tax = Zea mays RepID = B6SWT0_MAIZE (1e−163); MFS_1: Major FacilitatorSuperfamily (0.0021); PTR2: POT family 3 118.4 174361364 174363736(2.6e−80); GO_MF:GO:0005215, transporter activity# (0.0);GO_BP:GO:0006857, oligopeptide transport# (0.0); GO_CC:GO:0016020,membrane# (0.0) 351 Aspartate aminotransferase n = 3 Tax = AndropogoneaeRepID = B6TK79_MAIZE (0.0); Aminotran_1_2: Aminotransferase class I andII (2.6e−88); 3 118.4 174367844 174371254 GO_MF:GO:0030170, pyridoxalphosphate binding# (0.0); GO_BP:GO:0016847,1-aminocyclopropane-1-carboxylate synthase activity# (0.0);GO_CC:GO:0009507, chloroplast# (1e−179) 352 NPH3 family protein n = 3Tax = Oryza sativa Japonica Group RepID = Q6AST7_ORYSJ (1e−36);GO_MF:GO:0005515, protein binding# (6e−37); 3 118.4 174448279 174454420GO_BP:GO:0009416, IEP#response to light stimulus# (6e−37);GO_CC:GO:0005634, nucleus# (4e−33) 353 Nucleic acid binding protein n =4 Tax = Zea mays RepID = B6THI3_MAIZE (0.0); zf-C2H2: Zinc finger, C2H2type (2.3e−05); zf-C2H2: Zinc finger, C2H2 type 3 118.4 174461545174465281 (0.48); zf-C2H2: Zinc finger, C2H2 type (1.9);GO_MF:GO:0008270, zinc ion binding# (0.0); GO_BP:GO:0045449, regulationof transcription# (0.0); GO_CC:GO:0005622, intracellular# (0.0) 354Embryogenesis-associated protein EMB8 n = 2 Tax = Andropogoneae RepID =B6U4K5_MAIZE (0.0); Abhydrolase_1: alpha/beta hydrolase fold (3.6e−05);3 118.4 174533708 174546586 GO_MF:GO:0016787, hydrolase activity# (0.0);GO_BP:GO:0008150, ND#biological_process# (9e−44); GO_CC:GO:0016021,integral to membrane# (9e−44) 355 OSJNBa0079C19.15 protein n = 1 Tax =Oryza sativa RepID = Q7FA01_ORYSA (6e−45); GO_MF:GO:0004803, transposaseactivity# (6e−45); 3 118.4 174548132 174548512 GO_BP:GO:0006313,transposition, DNA-mediated# (6e−45); GO_CC:GO:0005783, IDA#endoplasmicreticulum# (1e−34) 356 Gag-pol polyprotein, putative n = 1 Tax =Asparagus officinalis RepID = Q2A9Z5_ASPOF (4e−12); GO_MF:GO:0003964,RNA-directed DNA polymerase, 3 118.4 174679875 174680057 group II intronencoded# (4e−12); GO_BP:GO:0006278, RNA-dependent DNA replication#(4e−12); GO_CC:GO:0005634, nucleus# (3e−10) 357 Retrotransposon protein,putative, Ty3-gypsy subclass n = 2 Tax = Oryza sativa RepID =Q7XG23_ORYSJ (2e−54); GO_MF:GO:0008270, zinc ion 3 118.4 174680227174681046 binding# (4e−55); GO_BP:GO:0015074, DNA integration# (4e−55);GO_CC:GO:0005634, nucleus# (2e−54) 358 X-ray repair cross complementingprotein 2, xrcc2, putative n = 1 Tax = Ricinus communis RepID =B9RPZ1_RICCO (3e−38); GO_MF:GO:0003677, DNA 3 118.4 174688476 174694367binding# (5e−49); GO_BP:GO:0006506, TAS#GPI anchor biosynthetic process#(7e−73); GO_CC:GO:0016021, integral to membrane# (7e−73) 359 MATE effluxprotein-like n = 2 Tax = Oryza sativa RepID = Q5N7N1_ORYSJ (3e−15);GO_MF:GO:0015297, antiporter activity# (1e−61); 3 118.4 174696169174699186 GO_BP:GO:0055085, transmembrane transport# (1e−61);GO_CC:GO:0016020, membrane# (1e−61) 360 Probable ion channel DMI1,chloroplastic n = 5 Tax = Oryza sativa RepID = DMI1_ORYSJ (0.0); TrkA_N:TrkA-N domain (0.023); DUF1012: Protein of unknown 3 118.4 174760419174769602 function (DUF1012) (4.7e−05); GO_MF:GO:0005488, binding#(0.0); GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0031969,IDA#chloroplast membrane# (0.0) 361 Serine/threonine-protein kinaseSAPK4 n = 5 Tax = Poaceae RepID = SAPK4_ORYSJ (1e−123); Pkinase: Proteinkinase domain (1.4e−64); Pkinase_Tyr: Protein 3 118.4 174770228174775694 tyrosine kinase (2.4e−12); GO_MF:GO:0016740, transferaseactivity# (1e−123); GO_BP:GO:0016301, kinase activity# (1e−123);GO_CC:GO:0005634, nucleus# (1e−108) 362 Photosystem II 22 kDa protein n= 3 Tax = Andropogoneae RepID = B6TKD1_MAIZE (1e−107); Chloroa_b-bind:Chlorophyll A-B binding protein (1.6e−17); 3 118.4 174825207 174827767GO_MF:GO:0051738, TAS#xanthophyll binding# (3e−70); GO_BP:GO:0015979,photosynthesis# (3e−74); GO_CC:GO:0016021, integral to membrane# (3e−74)363 P-type ATPase (Fragment) n = 1 Tax = Hordeum vulgare RepID =Q94IM9_HORVU (5e−50); Hydrolase: haloacid dehalogenase-like hydrolase(0.034); 3 118.4 174893873 174896216 GO_MF:GO:0015662, ATPase activity,coupled to transmembrane movement of ions, phosphorylative mechanism#(5e−50); GO_BP:GO:0015662, ATPase activity, coupled to transmembranemovement of ions, phosphorylative mechanism# (5e−50); GO_CC:GO:0016021,integral to membrane# (5e−50) 364 Putative uncharacterized proteinSb04g024240 n = 1 Tax = Sorghum bicolor RepID = C5XWC2_SORBI (1e−20);zf-CCHC: Zinc knuckle (0.0001) 3 118.4 174915097 174915526 365 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C0PGF1_MAIZE(7e−17) 3 118.7 175096376 175096756 366 Androgen induced inhibitor ofproliferation (As3)/pds5, putative n = 1 Tax = Ricinus communis RepID =B9RJ83_RICCO (3e−29); GO_MF:GO:0005488, 3 118.7 175099942 175103297binding# (8e−55); GO_BP:GO:0008152, metabolic process# (2e−16);GO_CC:GO:0009507, chloroplast# (4e−19) 367 Putative retroelement proteinn = 1 Tax = Sorghum bicolor RepID = B3VTC3_SORBI (8e−19);GO_MF:GO:0003682, chromatin binding# (8e−19); 3 118.7 175105093175105440 GO_BP:GO:0006333, chromatin assembly or disassembly# (8e−19);GO_CC:GO:0005634, nucleus# (8e−19) 368 Androgen induced inhibitor ofproliferation (As3)/pds5, putative n = 1 Tax = Ricinus communis RepID =B9RJ83_RICCO (2e−89); HEAT: HEAT repeat (7.1); HEAT: 3 118.7 175123678175125164 HEAT repeat (16); HEAT: HEAT repeat (4.5); HEAT: HEAT repeat(8.5); GO_MF:GO:0005488, binding# (1e−130); GO_BP:GO:0006278,RNA-dependent DNA replication# (1e−95); GO_CC:GO:0005634, nucleus#(6e−18) 369 Putative uncharacterized protein Sb03g041085 (Fragment) n =1 Tax = Sorghum bicolor RepID = C5XR84_SORBI (5e−56); GO_MF:GO:0005488,binding# (8e−26) 3 118.8 175183345 175192059 370 NPL4 family protein n =4 Tax = Andropogoneae RepID = B4FHK5_MAIZE (0.0); NPL4: NPL4 family(7.3e−135); GO_MF:GO:0005488, binding# (7e−97); 3 118.9 175281852175287153 GO_CC:GO:0005783, IDA#endoplasmic reticulum# (0.0) 371OSJNBa0065J03.2 protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q7X7Y2_ORYSJ (2e−18); GO_MF:GO:0004190, penicillopepsin activity#(2e−18); 3 119 175317626 175318015 GO_BP:GO:0015074, DNA integration#(2e−18); GO_CC:GO:0005634, nucleus# (2e−18) 372 Protein disulfideisomerase n = 2 Tax = Andropogoneae RepID = Q5EUD0_MAIZE (1e−46);GO_MF:GO:0016853, isomerase activity# (1e−46); 3 119 175325155 175326673GO_BP:GO:0045454, cell redox homeostasis# (1e−46); GO_CC:GO:0005783,IDA#endoplasmic reticulum# (2e−13) 373 Zeamatin, putative n = 1 Tax =Ricinus communis RepID = B9SZE5_RICCO (7e−28); Thaumatin: Thaumatinfamily (0.00013); GO_MF:GO:0005515, protein 3 119 175338982 175339505binding# (2e−10); GO_BP:GO:0046686, IEP#response to cadmium ion#(2e−10); GO_CC:GO:0005618, IDA#cell wall# (5e−24) 374 Ribonuclease P n =3 Tax = Andropogoneae RepID = B6T7F1_MAIZE (1e−17); GO_MF:GO:0003676,nucleic acid binding# (1e−17); GO_BP:GO:0006468, protein 3 119 175340760175343653 amino acid phosphorylation# (1e−09) 375 Retrotransposonprotein SINE subclass n = 3 Tax = Zea mays RepID = B6T9S6_MAIZE (6e−36);GO_MF:GO:0016787, hydrolase activity# (6e−36); 3 119 175343828 175348081GO_BP:GO:0006629, lipid metabolic process# (6e−36); GO_CC:GO:0005773,IDA#vacuole# (2e−20) 376 OSJNBa0064G10.15 protein n = 1 Tax = Oryzasativa Japonica Group RepID = Q7XKB6_ORYSJ (5e−37); DUF639: Plantprotein of unknown function (DUF639) 3 119 175348514 175374260(5.3e−258); Reticulon: Reticulon (0.013) 377 Retrotransposon protein,putative, Tyl-copia subclass n = 1 Tax = Oryza sativa Japonica GroupRepID = Q2QSG2_ORYSJ (1e−13); GO_MF:GO:0003677, DNA 3 119 175372096175372675 binding# (1e−13); GO_BP:GO:0015074, DNA integration# (1e−13)378 DNA polymerase n = 1 Tax = Sorghum bicolor RepID = C5XR76_SORBI(0.0); DNA_pol_B_exo: DNA polymerase family B, exonuclease domain(1.6e−12); 3 119.2 175498364 175514524 DNA_pol_B: DNA polymerase familyB (6.5e−174); zf-DNA_Pol: DNA Polymerase alpha zinc finger (1.6e−09);GO_MF:GO:0016779, nucleotidyltransferase activity# (0.0);GO_BP:GO:0006273, ISS#lagging strand elongation# (0.0);GO_CC:GO:0005634, nucleus# (0.0) 379 Palmitoyltransferase ZDHHC9 n = 3Tax = Andropogoneae RepID = B6TJT8_MAIZE (1e−107); zf-DHHC: DHHC zincfinger domain (9.6e−30); 3 119.2 175517630 175524776 GO_MF:GO:0046872,metal ion binding# (1e−113); GO_BP:GO:0006412, translation# (3e−87);GO_CC:GO:0016021, integral to membrane# (1e−84) 380 Tubulin gamma-1chain n = 28 Tax = Embryophyta RepID = TBG1_ARATH (5e−29);GO_MF:GO:0050660, FAD binding# (3e−33); GO_BP:GO:0055114, 3 119.2175525450 175528545 oxidation reduction# (3e−33); GO_CC:GO:0043234,protein complex# (3e−33) 381 AP2 domain transcription factor-like n = 2Tax = Oryza sativa RepID = Q5N965_ORYSJ (2e−34); AP2: AP2 domain(2.1e−20); GO_MF:GO:0003700, transcription 3 119.2 175549184 175552449factor activity# (2e−34); GO_BP:GO:0045449, regulation of transcription#(2e−34); GO_CC:GO:0005634, nucleus# (2e−34) 382 Membrane proteinCOV-like n = 5 Tax = Poaceae RepID = Q8S1P4_ORYSJ (1e−136); DUF502:Protein of unknown function (DUF502) (2.5e−36) 3 119.2 175556827175563188 383 Yip1 domain containing protein n = 1 Tax = Tetrahymenathermophila SB210 RepID = Q22B55_TETTH (6e−09); Yip1: Yip1 domain(0.0016) 3 119.2 175566419 175569742 384 Sterol3-beta-glucosyltransferase n = 3 Tax = Andropogoneae RepID =B6SKE1_MAIZE (0.0); Glyco_transf_28: Glycosyltransferase family 28N-terminal domain 3 119.2 175571486 175589373 (1.2e−46);GO_MF:GO:0016758, transferase activity, transferring hexosyl groups#(0.0); GO_BP:GO:0030259, lipid glycosylation# (0.0); GO_CC:GO:0005886,plasma membrane# (0.0) 385 G-box-binding factor 4 n = 3 Tax =Andropogoneae RepID = B6SKU0_MAIZE (7e−73); bZIP_1: bZIP transcriptionfactor (3.1e−11); bZIP_2: Basic region 3 119.3 175629663 175633484leucine zipper (5.6e−09); GO_MF:GO:0046983, protein dimerizationactivity# (7e−73); GO_BP:GO:0006355, regulation of transcription,DNA-dependent# (7e−73); GO_CC:GO:0005634, nucleus# (7e−73) 386 Putativeuncharacterized protein Sb03g040960 n = 2 Tax = Andropogoneae RepID =C5XQN1_SORBI (0.0); GO_MF:GO:0005524, ATP binding# (1e−94); 3 119.3175633118 175637510 GO_BP:GO:0006437, tyrosyl-tRNA aminoacylation#(1e−94); GO_CC:GO:0005737, cytoplasm# (1e−94) 387 Transferase n = 2 Tax= Zea mays RepID = B6TPL4_MAIZE (0.0); Transferase: Transferase family(3.6e−106); GO_MF:GO:0016747, transferase activity, 3 119.4 175686325175689363 transferring acyl groups other than amino-acyl groups# (0.0)388 Tubby-like protein n = 3 Tax = Andropogoneae RepID = B6U1N5_MAIZE(0.0); F-box: F-box domain (6.4e−06); Tub: Tub family (1.6e−168); 3119.4 175691664 175696010 GO_MF:GO:0016787, hydrolase activity#(1e−113); GO_BP:GO:0045449, regulation of transcription# (1e−154);GO_CC:GO:0005886, plasma membrane# (1e−154) 389 Snrnp sm protein,putative n = 6 Tax = Embryophyta RepID = B9T2G3_RICCO (5e−10) 3 119.4175701107 175701619 390 BolA-like protein n = 4 Tax = Poaceae RepID =Q5N9F3_ORYSJ (1e−29); BolA: BolA-like protein (6.9e−31) 3 119.4175705359 175706614 391 Fructose-1,6-bisphosphatase, cytosolic n = 5 Tax= Poaceae RepID = F16P2_ORYCO (0.0); FBPase: Fructose-1-6-bisphosphatase(5.4e−204); 3 119.4 175707507 175710858 GO_MF:GO:0046872, metal ionbinding# (0.0); GO_BP:GO:0042132, fructose 1,6-bisphosphate1-phosphatase activity# (0.0); GO_CC:GO:0005737, cytoplasm# (0.0) 392VAMP-like protein YKT62 n = 3 Tax = Zea mays RepID = B6TVP5_MAIZE(7e−69); GO_MF:GO:0042578, phosphoric ester hydrolase 3 119.6 175779152175780870 activity# (7e−58); GO_BP:GO:0016192, vesicle-mediatedtransport# (7e−69); GO_CC:GO:0016021, integral to membrane# (7e−69) 393Histone H3 n = 1 Tax = Oryza sativa Japonica Group RepID = Q53NE3_ORYSJ(6e−23); GO_MF:GO:0003677, DNA binding# (6e−23); 3 119.8 175868598175869269 GO_BP:GO:0006334, nucleosome assembly# (6e−23);GO_CC:GO:0005694, chromosome# (6e−23) 394 Peptidase M48, Ste24p n = 1Tax = Zea mays RepID = B6TA12_MAIZE (3e−60); Peptidase_M48: Peptidasefamily M48 (7.1e−07); Peptidase_M56: 3 120 175926976 175931743 BlaR1peptidase M56 (0.0091); Peptidase_C12: Ubiquitin carboxyl-terminalhydrolase, family 1 (0.00015); GO_MF:GO:0016787, hydrolase activity#(3e−60); GO_BP:GO:0006508, proteolysis# (3e−60); GO_CC:GO:0016020,membrane# (3e−60) 395 Surfactant protein B containing protein n = 3 Tax= Zea mays RepID = B6T8B6_MAIZE (2e−62); SapB_1: Saposin-like type B,region 1 (2.8e−05); SapB_2: 3 120.05 175931839 175935637 Saposin-liketype B, region 2 (0.028); GO_BP:GO:0006629, lipid metabolic process#(2e−62); GO_CC:GO:0005764, lysosome# (2e−15) 396 RING-H2 finger proteinATL3C n = 1 Tax = Zea mays RepID = B6ST75_MAIZE (2e−54); zf-C3HC4: Zincfinger, C3HC4 type (RING finger) (6.2e−06); 3 120.1 175936649 175937454GO_MF:GO:0046872, metal ion binding# (2e−54); GO_CC:GO:0016021, integralto membrane# (2e−15) 397 Dof zinc finger protein 9 n = 1 Tax = Hordeumvulgare subsp. vulgare RepID = A5HWF8_HORVD (2e−39); zf-Dof: Dof domain,zinc finger (1.2e−35); 3 120.2 175965582 175974373 GO_MF:GO:0008270,zinc ion binding# (2e−39); GO_BP:GO:0045449, regulation oftranscription# (2e−39); GO_CC:GO:0005634, nucleus# (2e−26) 398 Dof zincfinger protein 9 n = 1 Tax = Hordeum vulgare subsp. vulgare RepID =A5HWF8_HORVD (2e−48); GO_MF:GO:0008270, zinc ion binding# (2e−56); 3120.2 175965975 175966855 GO_BP:GO:0045449, regulation of transcription#(2e−56) 399 Anthocyanidin 3-O-glucosyltransferase n = 2 Tax =Andropogoneae RepID = B6SU01_MAIZE (0.0); UDPGT: UDP-glucoronosyl andUDP-glucosyl transferase 3 120.3 175998910 176000677 (1e−07);GO_MF:GO:0016758, transferase activity, transferring hexosyl groups#(0.0); GO_BP:GO:0008152, metabolic process# (0.0) 400 Anthocyanidin3-O-glucosyltransferase n = 2 Tax = Andropogoneae RepID = B6SU01_MAIZE(0.0); UDPGT: UDP-glucoronosyl and UDP-glucosyl transferase 3 120.3176038345 176039880 (2.4e−07); GO_MF:GO:0016758, transferase activity,transferring hexosyl groups# (0.0); GO_BP:GO:0008152, metabolic process#(0.0) 401 Anthocyanidin 3-O-glucosyltransferase n = 2 Tax =Andropogoneae RepID = B6TRK5_MAIZE (0.0); UDPGT: UDP-glucoronosyl andUDP-glucosyl transferase 3 120.3 176043286 176045138 (3.2e−07);Glyco_tran_28_C: Glycosyltransferase family 28 C-terminal domain(0.042); GO_MF:GO:0016758, transferase activity, transferring hexosylgroups# (0.0); GO_BP:GO:0008152, metabolic process# (0.0) 402 Putativeuncharacterized protein Sb06g011810 n = 1 Tax = Sorghum bicolor RepID =C5YEB3_SORBI (6e−11) 3 120.3 176051207 176051446 403Photoreceptor-interacting protein-like n = 4 Tax = Andropogoneae RepID =B6T0F2_MAIZE (2e−36); NPH3: NPH3 family (0.0099); GO_MF:GO:0004871,signal 3 120.3 176070424 176071202 transducer activity# (7e−37);GO_BP:GO:0009416, IEP#response to light stimulus# (7e−37);GO_CC:GO:0005886, plasma membrane# (4e−09) 404 Uricase n = 1 Tax = Zeamays RepID = B4G1P7_MAIZE (1e−149); Uricase: Uricase (7.8e−48); Uricase:Uricase (3.1e−20); GO_MF:GO:0016491, oxidoreductase 3 120.3 176257559176261095 activity# (1e−131); GO_BP:GO:0055114, oxidation reduction#(1e−131); GO_CC:GO:0005777, IDA#peroxisome# (4e−86) 405 Retrotransposonprotein, putative, Tyl-copia subclass n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q2QSQ2_ORYSJ (2e−24); GO_MF:GO:0008270, zinc ion 3 120.3176264306 176264540 binding# (2e−24); GO_BP:GO:0015074, DNA integration#(2e−24); GO_CC:GO:0005622, intracellular# (9e−19) 406Serine/threonine-protein kinase Nek5 n = 2 Tax = Oryza sativa RepID =NEK5_ORYSJ (0.0); Pkinase: Protein kinase domain (1.4e−75); Pkinase_Tyr:Protein 3 120.5 176289474 176295510 tyrosine kinase (2.9e−16); Kdo:Lipopolysaccharide kinase (Kdo) (0.024); GO_MF:GO:0016740, transferaseactivity# (0.0); GO_BP:GO:0016301, kinase activity# (0.0);GO_CC:GO:0055028, IDA#cortical microtubule# (1e−156) 407 Syntaxin,plant, putative n = 1 Tax = Ricinus communis RepID = B9T2S1_RICCO(4e−20); Hin1: Harpin-induced protein 1 (Hin1) (1.1e−34);GO_MF:GO:0000156, 3 120.5 176296694 176297398 two-component responseregulator activity# (6e−11); GO_BP:GO:0051607, IMP#defense response tovirus# (4e−20); GO_CC:GO:0005886, plasma membrane# (4e−20) 408Harpin-induced protein n = 1 Tax = Bruguiera gymnorhiza RepID =B1Q4T2_9ROSI (7e−14); Hin1: Harpin-induced protein 1 (Hin1) (3.4e−19); 3120.6 176301641 176302213 GO_BP:GO:0051707, IEP#response to otherorganism# (1e−12); GO_CC:GO:0009507, chloroplast# (1e−12) 409Hairpin-inducing protein n = 1 Tax = Casuarina glauca RepID =B0ZC11_CASGL (4e−21); Hin1: Harpin-induced protein 1 (Hin1) (1.1e−42);GO_MF:GO:0005515, 3 120.6 176302930 176304103 protein binding# (3e−10);GO_BP:GO:0051607, IMP#defense response to virus# (1e−16);GO_CC:GO:0005886, plasma membrane# (1e−16) 410 Putative uncharacterizedprotein Sb03g040780 n = 1 Tax = Sorghum bicolor RepID = C5XQL0_SORBI(1e−18) 3 120.9 176322098 176322610 411 Ovate protein n = 1 Tax = Zeamays RepID = B6SI20_MAIZE (3e−11); DUF623: Protein of unknown function,DUF623 (1.4e−34) 3 121 176332104 176333800 412 OSJNBa0088H09.12 proteinn = 3 Tax = Oryza sativa RepID = Q7XPU4_ORYSJ (2e−14); DUF623: Proteinof unknown function, DUF623 (1.1e−30) 3 121.3 176354609 176356292 413Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6TX28_MAIZE (2e−38) 3 121.5 176365418 176366596 414 RNA binding proteinn = 3 Tax = Andropogoneae RepID = B6T390_MAIZE (1e−09); PAM2: Ataxin-2C-terminal region (3.8e−05); GO_MF:GO:0003723, RNA 3 121.5 176373937176374758 binding# (1e−09); GO_BP:GO:0006396, RNA processing# (1e−09);GO_CC:GO:0030529, ribonucleoprotein complex# (1e−09) 415 Putativegag-pol polyprotein n = 1 Tax = Zea mays RepID = Q8H6I4_MAIZE (1e−56);GO_MF:GO:0008270, zinc ion binding# (1e−56); GO_BP:GO:0015074, 3 121.5176425086 176425704 DNA integration# (1e−56) 416 DNA binding protein n =2 Tax = Andropogoneae RepID = B6U2E0_MAIZE (1e−50); Myb_DNA-binding:Myb-like DNA-binding domain (0.043); 3 121.5 176433983 176435494Myb_DNA-binding: Myb-like DNA-binding domain (3.3e−12);GO_MF:GO:0003677, DNA binding# (9e−59); GO_BP:GO:0045449, regulation oftranscription# (1e−39); GO_CC:GO:0005634, nucleus# (1e−54) 417 Sortingnexin 3, putative n = 1 Tax = Ricinus communis RepID = B9SWH8_RICCO(1e−168); PX: PX domain (1.7e−32); Vps5: Vps5 C terminal like (5e−15); 3121.6 176446897 176451221 GO_MF:GO:0035091, IMP#phosphoinositidebinding# (0.0); GO_BP:GO:0007154, cell communication# (0.0);GO_CC:GO:0030904, retromer complex# (1e−163) 418 Putativeuncharacterized protein Sb03g040710 n = 2 Tax = Andropogoneae RepID =C5XQK3_SORBI (6e−76); DUF584: Protein of unknown function, DUF584 3121.65 176451411 176452570 (1.5e−17) 419 NAC transcription factor-likeprotein n = 2 Tax = Oryza sativaRepID = Q94CW0_ORYSJ (3e−69); NAM: Noapical meristem (NAM) protein (2.1e−47); 3 122.15 176510723 176512231GO_MF:GO:0003677, DNA binding# (3e−69); GO_BP:GO:0045449, regulation oftranscription# (3e−69); GO_CC:GO:0005634, nucleus# (7e−51) 420 Putativeuncharacterized protein Sb03g040650 n = 1 Tax = Sorghum bicolor RepID =C5XQJ7_SORBI (1e−35) 3 122.15 176553306 176554086 421 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6TW48_MAIZE(2e−76) 3 122.2 176556622 176558592 422 Catalytic/hydrolase n = 2 Tax =Andropogoneae RepID = B6SJL9_MAIZE (0.0); PGAP1: PGAP1-like protein(0.071); Abhydrolase_1: alpha/beta hydrolase fold 3 122.25 176523462176526731 (0.00056); GO_MF:GO:0016787, hydrolase activity# (0.0);GO_BP:GO:0006066, alcohol metabolic process# (1e−154); GO_CC:GO:0005739,mitochondrion# (1e−105) 423 Phospholipase D alpha 1 n = 2 Tax =Andropogoneae RepID = PLDA1_MAIZE (9e−28); PPR: PPR repeat (0.01);Glyco_hydro_9: Glycosyl hydrolase family 9 3 122.3 176528107 176529876(1.6e−05); GO_MF:GO:0004553, hydrolase activity, hydrolyzing O-glycosylcompounds# (0.0); GO_BP:GO:0005975, carbohydrate metabolic process#(0.0); GO_CC:GO:0016020, membrane# (9e−28) 424 Putative uncharacterizedprotein Sb03g040660 n = 1 Tax = Sorghum bicolor RepID = C5XQJ8_SORBI(0.0); Hemerythrin: Hemerythrin (0.015); Hemerythrin: 3 122.3 176529882176533815 Hemerythrin (2.3); DUF1054: Protein of unknown function(DUF1054) (0.016); GO_MF:GO:0005515, protein binding# (1e−35) 425Hevamine-A n = 1 Tax = Zea mays RepID = B6TVA3_MAIZE (1e−145);Glyco_hydro_18: Glycosyl hydrolases family 18 (1.5e−51);GO_MF:GO:0043169, cation 3 122.4 176569917 176571499 binding# (1e−145);GO_BP:GO:0008152, metabolic process# (1e−145); GO_CC:GO:0005773,IDA#vacuole# (1e−97) 426 Class III chitinase n = 1 Tax = Panax ginsengRepID = Q19AL0_PANGI (1e−102); Glyco_hydro_18: Glycosyl hydrolasesfamily 18 (3.9e−44); 3 122.4 176597593 176598560 GO_MF:GO:0043169,cation binding# (1e−133); GO_BP:GO:0008152, metabolic process# (1e−133);GO_CC:GO:0005615, extracellular space# (1e−96) 427 Class III chitinase n= 1 Tax = Panax ginseng RepID = Q19AL0_PANGI (1e−105); Glyco_hydro_18:Glycosyl hydrolases family 18 (2.9e−52); 3 122.4 176599914 176600977GO_MF:GO:0043169, cation binding# (1e−137); GO_BP:GO:0008152, metabolicprocess# (1e−137); GO_CC:GO:0005618, IDA#cell wall# (2e−97) 428 Heatshock protein binding protein n = 1 Tax = Zea mays RepID = B6U723_MAIZE(5e−32); GO_MF:GO:0031072, heat shock protein binding# (5e−32); 3 122.4176777440 176778989 GO_BP:GO:0006950, response to stress# (5e−32) 429Putative uncharacterized protein Sb02g037540 n = 2 Tax = AndropogoneaeRepID = C5XCF9_SORBI (6e−17) 3 122.4 176778705 176782511 430 F-boxprotein-like n = 4 Tax = Poaceae RepID = Q8RZ32_ORYSJ (0.0); F-box:F-box domain (0.0046) 3 122.4 176783173 176791091 431 Putativeuncharacterized protein Sb02g042500 n = 1 Tax = Sorghum bicolor RepID =C5X4Y2_SORBI (1e−17); PPR: PPR repeat (1e−06) 3 122.4 176788513176788932 432 Glucan endo-1,3-beta-glucosidase 7 n = 2 Tax =Andropogoneae RepID = B6T478_MAIZE (1e−179); Glyco_hydro_17: Glycosylhydrolases family 17 (1.7e−96); 3 122.45 176562644 176565454GO_MF:GO:0043169, cation binding# (1e−179); GO_BP:GO:0008152, metabolicprocess# (1e−179); GO_CC:GO:0046658, anchored to plasma membrane#(1e−107) 433 Liguleless2-like protein n = 2 Tax = Zea mays RepID =Q84UA5_MAIZE (0.0); bZIP_2: Basic region leucine zipper (0.01); bZIP_1:bZIP transcription factor 3 122.75 176800252 176808864 (0.003);GO_MF:GO:0046983, protein dimerization activity# (0.0);GO_BP:GO:0006355, regulation of transcription, DNA-dependent# (0.0);GO_CC:GO:0005634, nucleus# (0.0) 434 Putative uncharacterized protein n= 2 Tax = Zea mays RepID = B6U713_MAIZE (0.0); 2OG-FeII_Oxy: 2OG-Fe(II)oxygenase superfamily (0.018); 8 69 66655758 66661595 GO_MF:GO:0016491,oxidoreductase activity# (0.0); GO_BP:GO:0055114, oxidation reduction#(0.0) 435 Phragmoplastin n = 1 Tax = Camellia sinensis RepID =A2T1L8_CAMSI (0.0); MMR_HSR1: GTPase of unknown function (0.00019);Dynamin_N: Dynamin 8 69 66662515 66672012 family (5.5e−88); Dynamin_M:Dynamin central region (1.2e−103); GED: Dynamin GTPase effector domain(3.3e−38); GO_MF:GO:0005525, GTP binding# (0.0); GO_BP:GO:0051301, celldivision# (0.0); GO_CC:GO:0009524, IDA#phragmoplast# (0.0) 436 Dopaminebeta-monooxygenase n = 3 Tax = Andropogoneae RepID = B6TH76_MAIZE (0.0);DOMON: DOMON domain (0.025); DUF2427: Domain of unknown 8 69 6682980466832512 function (DUF2427) (0.082); GO_MF:GO:0004500, dopaminebeta-monooxygenase activity# (0.0); GO_BP:GO:0006548, histidinecatabolic process# (0.0); GO_CC:GO:0016021, integral to membrane#(1e−44) 437 Dopamine beta-monooxygenase n = 3 Tax = Andropogoneae RepID= B6TH76_MAIZE (0.0); DOMON: DOMON domain (0.025); DUF2427: Domain ofunknown 8 69 66856185 66858893 function (DUF2427) (0.082);GO_MF:GO:0004500, dopamine beta-monooxygenase activity# (0.0);GO_BP:GO:0006548, histidine catabolic process# (0.0); GO_CC:GO:0016021,integral to membrane# (1e−44) 438 Putative uncharacterized protein n = 1Tax = Zea mays RepID = C0HDR6_MAIZE (1e−126); DUF1639: Protein ofunknown function (DUF1639) (9.5e−29) 8 69 66988051 66992509 439 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C4J1U2_MAIZE(4e−10) 8 69 66992846 66993175 440 Chloroplast nucleoid DNA bindingprotein-like n = 1 Tax = Oryza sativa Japonica Group RepID =Q5QM88_ORYSJ (8e−82); Asp: Eukaryotic aspartyl protease 8 69 6720270267205538 (0.00063); GO_MF:GO:0016787, hydrolase activity# (1e−163);GO_BP:GO:0006508, proteolysis# (1e−163) 441 Putative uncharacterizedprotein Sb09g028090 n = 2 Tax = Andropogoneae RepID = C5YVF9_SORBI(0.0); GO_CC:GO:0009507, chloroplast# (1e−102) 8 69 67353996 67357167442 RNA Binding Protein-like n = 2 Tax = Oryza sativa Japonica GroupRepID = Q7EZ58_ORYSJ (3e−43); RAM_1: RNA recognition motif. (a.k.a. RRM,RB (0.06); 8 69 67374077 67376254 RRM_1: RNA recognition motif. (a.k.a.RRM, RB (0.011); GO_MF:GO:0003676, nucleic acid binding# (8e−62) 443Glycoside hydrolase, family 28 n = 2 Tax = Andropogoneae RepID =B6TX01_MAIZE (2e−39); GO_MF:GO:0016798, hydrolase activity, acting onglycosyl 8 69 67378682 67379061 bonds# (2e−39); GO_BP:GO:0008152,metabolic process# (2e−39); GO_CC:GO:0016021, integral to membrane#(1e−21) 444 Ubiquitin-conjugating enzyme E2 n = 3 Tax = AndropogoneaeRepID = Q5RLI8_MAIZE (1e−162); UQ_con: Ubiquitin-conjugating enzyme(1.6e−28); 8 69 67380589 67387935 GO_MF:GO:0019787, small conjugatingprotein ligase activity# (0.0); GO_BP:GO:0051246, regulation of proteinmetabolic process# (0.0) 445 Pirin-like protein n = 1 Tax = Solanumlycopersicum RepID = PIRL_SOLLC (4e−26); Pirin: Pirin (4.2e−19);GO_MF:GO:0005516, IDA#calmodulin 8 69 67407549 67407962 binding#(2e−21); GO_BP:GO:0007018, microtubule-based movement# (4e−18);GO_CC:GO:0005634, nucleus# (4e−26) 446 Pirin-like protein n = 3 Tax =Zea mays RepID = B6TS20_MAIZE (2e−72); Pirin_C: Pirin C-terminal region(3.7e−38); GO_MF:GO:0005516, IDA#calmodulin 8 69 67407993 67410918binding# (1e−44); GO_BP:GO:0009738, IMP#abscisic acid mediated signalingpathway# (7e−43); GO_CC:GO:0005634, nucleus# (5e−50) 447 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C0P2F7_MAIZE(1e−22) 8 69 67415431 67415880 448 PHD-type zinc finger protein-like n =1 Tax = Oryza sativa Japonica Group RepID = Q84NP5_ORYSJ (2e−19);GO_MF:GO:0008080, N-acetyltransferase activity# 8 69 67417257 67417523(2e−22); GO_BP:GO:0008152, metabolic process# (2e−22) 449 TPR domaincontaining protein n = 2 Tax = Andropogoneae RepID = B6SI86_MAIZE(5e−66); GO_MF:GO:0005488, binding# (2e−16) 8 69 67445895 67448009 45050S ribosomal protein L21 n = 4 Tax = Andropogoneae RepID = C5YVG2_SORBI(1e−54); Ribosomal_L21p:Ribosomal prokaryotic L21 protein (2.8e−27); 869 67491320 67495907 GO_MF:GO:0019843, rRNA binding# (5e−43);GO_BP:GO:0006412, translation# (5e−43); GO_CC:GO:0030529,ribonucleoprotein complex# (5e−43) 451 Structural constituent ofribosome n = 1 Tax = Zea mays RepID = B6SGX4_MAIZE (0.0);GO_MF:GO:0003723, RNA binding# (0.0); GO_BP:GO:0006396, 8 69 6750003467501797 RNA processing# (0.0); GO_CC:GO:0016020, membrane# (7e−50) 45240S ribosomal protein S4 n = 17 Tax = commelinids RepID = RS4_ORYSJ(1e−30); Ribosomal_S4e: Ribosomal family S4e (6e−17); KOW: KOW motif(5.9e−05); 8 69 67556204 67557043 GO_MF:GO:0003735, structuralconstituent of ribosome# (3e−33); GO_BP:GO:0006412, translation#(3e−33); GO_CC:GO:0005840, ribosome# (3e−33) 453 Transposon protein,putative, CACTA, En/Spm sub-class n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q2R337_ORYSJ (2e−41); GO_MF:GO:0005524, ATP 8 69 7061500570615933 binding# (5e−24); GO_BP:GO:0006468, protein amino acidphosphorylation# (5e−24) 454 Transposon protein, putative, CACTA, En/Spmsub-class n = 1 Tax = Oryza sativa Japonica Group RepID = Q2QWY8_ORYSJ(1e−54); GO_MF:GO:0005524, ATP 8 69 70616774 70617362 binding# (8e−45);GO_BP:GO:0006468, protein amino acid phosphorylation# (8e−45) 455 CACTATnpD transposase n = 1 Tax = Zea mays RepID = A0EVJ2_MAIZE (8e−71);GO_MF:GO:0003677, DNA binding# (1e−35); GO_BP:GO:0015074, DNA 8 6970621129 70622573 integration# (1e−35) 456 Ubiquitin ligase proteincop1, putative n = 1 Tax = Ricinus communis RepID = B9RCP1_RICCO (0.0);Pkinase:Protein kinase domain (0.0091); WD40: WD 8 69 72174759 72182919domain, G-beta repeat (1.2); WD40: WD domain, G-beta repeat (0.016);WD40: WD domain, G-beta repeat (1.8e−07); WD40: WD domain, G-beta repeat(0.087); WD40: WD domain, G-beta repeat (5.4e−06); WD40: WD domain,G-beta repeat (5.1); GO_MF:GO:0032440, 2-alkenal reductase activity#(0.0); GO_BP:GO:0055114, oxidation reduction# (0.0); GO_CC:GO:0016607,IDA#nuclear speck# (0.0) 457 Protein disulfide isomerase n = 2 Tax =Andropogoneae RepID = Q5EUD6_MAIZE (2e−63); Thioredoxin: Thioredoxin(7.5e−13); ERp29: Endoplasmic reticulum 8 69 73202962 73205959 proteinERp29, C-te (0.035); GO_MF:GO:0016853, isomerase activity# (2e−63);GO_BP:GO:0045454, cell redox homeostasis# (2e−63); GO_CC:GO:0005783,IDA#endoplasmic reticulum# (2e−63) 458 UPF0737 protein 7 n = 3 Tax =Andropogoneae RepID = U7377_MAIZE (0.0); GO_MF:GO:0003676, nucleic acidbinding# (6e−09); GO_BP:GO:0006810, 8 69.1 67718099 67723430 transport#(6e−09); GO_CC:GO:0005634, nucleus# (0.0) 459 Os02g0140101 protein n = 4Tax = Poaceae RepID = Q6Z2X5_ORYSJ (3e−13); DPM2: Dolicholphosphate-mannose biosynthesis regu (9.5e−51); 8 69.1 67723481 67729604GO_MF:GO:0016757, transferase activity, transferring glycosyl groups#(3e−13) 460 Exosome complex exonuclease RRP41 n = 6 Tax = Poaceae RepID= B6T763_MAIZE (1e−105); RNase_PH: 3′ exoribonuclease family, domain 1(1.3e−21); 8 69.1 67729947 67735083 RNase_PH_C: 3′exoribonucleasefamily, domain 2 (2.9e−10); GO_MF:GO:0004527, exonuclease activity#(1e−105); GO_BP:GO:0006396, RNA processing# (1e−105); GO_CC:GO:0005737,cytoplasm# (3e−43) 461 OSJNBa0008M17.5 protein n = 1 Tax = Oryza sativaJaponica Group RepID = Q7XN10_ORYSJ (5e−36) 8 69.1 70603256 70603871 462Putative transposase n = 1 Tax = Zea mays RepID = Q945K3_MAIZE (1e−133);Transposase_11: Transposase DDE domain (0.001); Plant_tran: Planttransposon 8 69.1 73227996 73229586 protein (0.0001); GO_MF:GO:0004803,transposase activity# (1e−133); GO_BP:GO:0006313, transposition,DNA-mediated# (1e−133) 463 60S acidic ribosomal protein P2A n = 11 Tax =Andropogoneae RepID = RLA2A_MAIZE (5e−27); Ribosomal_60s: 60s Acidicribosomal protein (8e−38); 8 69.1 73231852 73233748 GO_MF:GO:0003735,structural constituent of ribosome# (5e−27); GO_BP:GO:0006414,translational elongation# (5e−27); GO_CC:GO:0030529, ribonucleoproteincomplex# (5e−27) 464 Retrotransposon protein, putative, unclassified n =2 Tax = Oryza sativa Japonica Group RepID = Q10HY9_ORYSJ (2e−15);GO_MF:GO:0008270, zinc ion 8 69.2 71728584 71730308 binding# (2e−19);GO_BP:GO:0006278, RNA-dependent DNA replication# (2e−15) 465Acyltransferase, putative n = 1 Tax = Ricinus communis RepID =B9RBF7_RICCO (0.0); Chal_sti_synt_N: Chalcone and stilbene synthases,N-te (0.075); 8 69.4 71791503 71793883 FAE1_CUT1_RppA: FAE1/Type IIIpolyketide synthase-lik (8.4e−226); ACP_syn III:3-Oxoacyl-[acyl-carrier-protein (ACP) (0.0054); Chal_sti_synt_C:Chalcone and stilbene synthases, C-te (1.2e−05); ACP_syn_III_C:3-Oxoacyl-[acyl-carrier-protein (ACP) (3.1e−07); GO_MF:GO:0016747,transferase activity, transferring acyl groups other than amino-acylgroups# (0.0); GO_BP:GO:0008610, lipid biosynthetic process# (0.0);GO_CC:GO:0016020, membrane# (0.0) 466 Glycerol kinase n = 2 Tax =Andropogoneae RepID = B6TZ71_MAIZE (9e−20); GO_MF:GO:0016773,phosphotransferase activity, alcohol group as acceptor# 8 69.4 7180856971809069 (9e−20); GO_BP:GO:0016301, kinase activity# (9e−20) 467DRE-binding protein DREB1 n = 1 Tax = Cymbidium insigne RepID =A6YT27_9ASPA (5e−24); AP2: AP2 domain (3.8e−20); GO_MF:GO:0003700, 869.5 72423591 72424384 transcription factor activity# (2e−67);GO_BP:GO:0045449, regulation of transcription# (2e−67);GO_CC:GO:0005634, nucleus# (2e−67) 468 ATP binding protein, putative n =1 Tax = Ricinus communis RepID = B9RCJ8_RICCO (0.0); AAA_3: ATPasefamily associated with various (0.0048); AAA: 8 69.6 75066604 75072021ATPase family associated with various cellular activities (AAA)(2.5e−78); AAA_5: ATPase family associated with various (0.0016);GO_MF:GO:0017111, nucleoside-triphosphatase activity# (0.0);GO_BP:GO:0051301, cell division# (0.0) 469 BRASSINOSTEROID INSENSITIVE1-associated receptor kinase 1, putative n = 1 Tax = Ricinus communisRepID = B9T3S1_RICCO (9e−25); 8 69.6 75073037 75075203 GO_MF:GO:0005524,ATP binding# (3e−28); GO_BP:GO:0006468, protein amino acidphosphorylation# (3e−28); GO_CC:GO:0016021, integral to membrane#(9e−25) 470 Transposon protein, putative, Mutator sub-class n = 1 Tax =Oryza sativa Japonica Group RepID = Q10FB0_ORYSJ (7e−16);GO_MF:GO:0008270, zinc ion 8 69.6 75165143 75165609 binding# (8e−16) 471Putative uncharacterized protein Sb09g029910 n = 2 Tax = Sorghum bicolorRepID = C5YWJ1_SORBI (6e−16); GO_BP:GO:0055085, transmembrane transport#8 69.6 75187314 75187850 (2e−12); GO_CC:GO:0016021, integral tomembrane# (2e−12) 472 Cyclin G-associated kinase-like protein n = 2 Tax= Oryza sativa RepID = Q69L76_ORYSJ (3e−74); GO_MF:GO:0016301, kinaseactivity# (3e−74); 8 69.65 74162123 74163697 GO_BP:GO:0016301, kinaseactivity# (3e−74) 473 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = B4FS85_MAIZE (1e−29) 8 69.7 72479615 72480599 474 Potassiumtransporter n = 3 Tax = Phragmites australis RepID = Q1T759_PHRAU(4e−13); GO_MF:GO:0015079, potassium ion transmembrane transporter 869.7 74163982 74164446 activity# (4e−13); GO_BP:GO:0015079, potassiumion transmembrane transporter activity# (4e−13); GO_CC:GO:0016020,membrane# (4e−13) 475 Formamidase n = 1 Tax = Lupinus albus RepID =B9VXW6_LUPAL (4e−37); FmdA_AmdA: Acetamidase/Formamidase family (0.063);GO_MF:GO:0016811, 8 69.75 71844016 71846415 hydrolase activity, actingon carbon-nitrogen (but not peptide) bonds, in linear amides# (4e−77);GO_BP:GO:0008152, metabolic process# (4e−77); GO_CC:GO:0005773,IDA#vacuole# (3e−38) 476 Protein Rf1, mitochondrial n = 2 Tax = Oryzasativa Indica Group RepID = RF1_ORYSI (0.0); PPR: PPR repeat (5.7); PPR:PPR repeat (0.69); PPR: PPR repeat 8 69.8 77089017 77091404 (0.00073);PPR: PPR repeat (8.3e−12); PPR: PPR repeat (4.7e−10); PPR: PPR repeat(5.4e−13); PPR: PPR repeat (7e−10); PPR: PPR repeat (0.034); PPR: PPRrepeat (9.4e−10); PPR: PPR repeat (2.9e−09); PPR: PPR repeat (2e−08);PPR: PPR repeat (1.6e−05); PPR: PPR repeat (1.9e−10); PPR: PPR repeat(1.3e−09); PPR: PPR repeat (0.00031); PPR: PPR repeat (5.3e−08); PPR:PPR repeat (3.6e−08); PPR: PPR repeat (4.8e−06); PPR: PPR repeat (4.6);GO_MF:GO:0008415, acyltransferase activity# (0.0); GO_BP:GO:0008152,metabolic process# (0.0); GO_CC:GO:0005739, mitochondrion# (0.0) 477 IMPdehydrogenase/GMP reductase, putative n = 1 Tax = Medicago truncatulaRepID = A2Q539_MEDTR (2e−11); GO_MF:GO:0003677, DNA binding# (1e−117) 869.8 77095435 77098145 478 WRKY transcription factor 19 n = 3 Tax =Oryza sativa Japonica Group RepID = Q6IER2_ORYSJ (7e−41); WRKY: WRKYDNA-binding domain (9.4e−30); 8 70.05 72382890 72384377GO_MF:GO:0043565, sequence-specific DNA binding# (7e−41);GO_BP:GO:0045449, regulation of transcription# (7e−41);GO_CC:GO:0005634, nucleus# (7e−41) 479 60S acidic ribosomal protein P2An = 11 Tax = Andropogoneae RepID = RLA2A_MAIZE (4e−30); Ribosomal_60s:60s Acidic ribosomal protein (1.2e−38); 8 70.05 73386619 73388908GO_MF:GO:0003735, structural constituent of ribosome# (4e−30);GO_BP:GO:0006414, translational elongation# (4e−30); GO_CC:GO:0030529,ribonucleoprotein complex# (4e−30) 480 Mitochondrial chaperone BCS1 n =3 Tax = Andropogoneae RepID = B6SVD2_MAIZE (1e−178); AAA: ATPase familyassociated with various cellular activities 8 70.05 77133844 77135045(AAA) (3.8e−10); AAA_5: ATPase family associated with various (0.014);GO_MF:GO:0017111, nucleoside-triphosphatase activity# (1e−178);GO_CC:GO:0005739, mitochondrion# (1e−106) 481 Mitogen-activated proteinkinase 9 n = 3 Tax = Oryza sativa RepID = MPK9_ORYSJ (1e−136); Pkinase:Protein kinase domain (0.0014); GO_MF:GO:0016740, 8 70.1 7468154574688835 transferase activity# (1e−136); GO_BP:GO:0016301, kinaseactivity# (1e−136); GO_CC:GO:0005886, plasma membrane# (3e−95) 482Putative polyprotein n = 1 Tax = Zea mays RepID = Q8SA93_MAIZE (1e−28);GO_MF:GO:0003964, RNA-directed DNA polymerase, group II intron encoded#8 70.1 76985420 76985803 (1e−28); GO_BP:GO:0006355, regulation oftranscription, DNA-dependent# (2e−29); GO_CC:GO:0005634, nucleus#(2e−29) 483 Mitochondrial chaperone BCS1 n = 3 Tax = Andropogoneae RepID= B6SVD2_MAIZE (0.0); AAA: ATPase family associated with variouscellular activities (AAA) 8 70.1 76990850 76992377 (7.7e−11); AAA_5:ATPase family associated with various (0.028); GO_MF:GO:0017111,nucleoside-triphosphatase activity# (0.0); GO_CC:GO:0005739,mitochondrion# (1e−106) 484 Mitochondrial chaperone BCS1 n = 3 Tax =Andropogoneae RepID = B6SVD2_MAIZE (2e−41); GO_MF:GO:0017111nucleoside-triphosphatase activity# (2e−41) 8 70.1 77135050 77135358 485IMP dehydrogenase/GMP reductase, putative n = 1 Tax = Medicagotruncatula RepID = A2Q539_MEDTR (5e−18); Myb_DNA-binding: Myb-likeDNA-binding 8 70.15 76983379 76988331 domain (0.072); GO_MF:GO:0003677,DNA binding# (0.0) 486 Tetratricopeptide repeat protein, tpr, putative n= 1 Tax = Ricinus communis RepID = B9R838_RICCO (7e−20); efhand: EF hand(3.9e−05); GO_MF:GO:0008270, 8 70.2 72620245 72622319 zinc ion binding#(3e−24); GO_BP:GO:0006508, proteolysis# (2e−14); GO_CC:GO:0000502,proteasome complex# (7e−20) 487 Probable protein phosphatase 2C 51 n = 3Tax = Oryza sativa RepID = P2C51_ORYSJ (2e−93); PP2C: Proteinphosphatase 2C (1.1e−76); 8 70.2 72623215 72631055 GO_MF:GO:0046872,metal ion binding# (1e−161); GO_BP:GO:0006470, protein amino aciddephosphorylation# (1e−161); GO_CC:GO:0008287, protein serine/threoninephosphatase complex# (1e−161) 488 ATP10 protein n = 1 Tax = Zea maysRepID = B6T7N8_MAIZE (1e−139); ATP-synt_10: ATP10 protein (3.7e−06);GO_MF:GO:0008236, serine-type peptidase 8 70.2 80037657 80046134activity# (1e−47); GO_BP:GO:0033615, mitochondrial proton-transportingATP synthase complex assembly# (1e−139); GO_CC:GO:0005743, mitochondrialinner membrane# (1e−139) 489 Cell division protein kinase, putative n =1 Tax = Ricinus communis RepID = B9T5N4_RICCO (7e−82); Pkinase_Tyr:Protein tyrosine kinase (1e−04); Pkinase: 8 70.25 80024603 80025821Protein kinase domain (5.4e−21); GO_MF:GO:0005524, ATP binding# (2e−95);GO_BP:GO:0006468, protein amino acid phosphorylation# (2e−95);GO_CC:GO:0005886, plasma membrane# (3e−82) 490 DNA binding protein n = 3Tax = Andropogoneae RepID = B6TCD9_MAIZE (9e−75); SRF-TF: SRF-typetranscription factor (DNA-binding and dimerisation 8 70.3 7265391072654861 domain) (8.5e−21); GO_MF:GO:0043565, sequence-specific DNAbinding# (9e−75); GO_BP:GO:0045449, regulation of transcription#(9e−75); GO_CC:GO:0005634, nucleus# (9e−75) 491 F-box domain containingprotein n = 3 Tax = Andropogoneae RepID = B6U5X4_MAIZE (6e−90) 8 70.372664145 72664967 492 DNA-binding protein WRKY3-like n = 2 Tax = Oryzasativa Japonica Group RepID = Q5ZEB2_ORYSJ (3e−37); WRKY: WRKYDNA-binding domain (4.7e−30); 8 70.35 74902354 74903632GO_MF:GO:0043565, sequence-specific DNA binding# (8e−48);GO_BP:GO:0045449, regulation of transcription# (8e−48);GO_CC:GO:0005634, nucleus# (8e−48) 493 Lactoylglutathione lyase n = 1Tax = Zea mays RepID = B4FQ23_MAIZE (1e−109); Glyoxalase:Glyoxalase/Bleomycin resistance protein/Dioxygenase 8 70.35 8029181380295002 superfamily (2.6e−11); GO_MF:GO:0016829, lyase activity#(1e−109) 494 Nucleoside diphosphate kinase n = 3 Tax = Zea mays RepID =B6TKC5_MAIZE (2e−72); NDK: Nucleoside diphosphate kinase (6e−65); 8 70.479074614 79075596 GO_MIF:GO:0016740, transferase activity# (2e−72);GO_BP:GO:0016301, kinase activity# (2e−72); GO_CC:GO:0005737, cytoplasm#(2e−72) 495 Alpha-N-arabinofuranosidase A n = 2 Tax = Zea mays RepID =B6T9B9_MAIZE (2e−78); GO_MF:GO:0046556, alpha-N-arabinofuranosidaseactivity# (2e−78); 8 70.4 80019137 80024134 GO_BP:GO:0046373,L-arabinose metabolic process# (2e−78); GO_CC:GO:0005578, proteinaceousextracellular matrix# (6e−54) 496 ATP binding protein n = 2 Tax =Andropogoneae RepID = B6SXM5_MAIZE (0.0); Kinesin: Kinesin motor domain(1.1e−125); GO_MF:GO:0005524, ATP binding# 8 70.45 83046589 83087386(0.0); GO_BP:GO:0007018, microtubule-based movement# (0.0);GO_CC:GO:0005874, microtubule# (0.0) 497 CTP synthase n = 1 Tax = Zeamays RepID = B6SXZ9_MAIZE (0.0); CTP_synth_N: CTP synthase N-terminus(4.4e−201); GATase:Glutamine amidotransferase 8 70.5 72744835 72751237class-I (3.3e−78); GO_MF:GO:0003883, CTP synthase activity# (0.0);GO_BP:GO:0006221, pyrimidine nucleotide biosynthetic process# (0.0);GO_CC:GO:0005829, IDA#cytosol# (1e−175) 498 OSJNBa0029L02.20 protein n =1 Tax = Oryza sativa RepID = Q7XMR7_ORYSA (6e−29); GO_MF:GO:0004523,ribonuclease H activity# (6e−29); 8 70.5 82966507 82967100GO_BP:GO:0045449, regulation of transcription# (6e−29) 499 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B7ZYQ1_MAIZE(1e−15) 8 70.5 83080991 83081326 500 Transposon protein, putative,CACTA, En/Spm sub-class n = 1 Tax = Oryza sativa Japonica Group RepID =Q2R1M9_ORYSJ (8e−60); GO_MF:GO:0004803, 8 70.55 74905264 74907420transposase activity# (2e−66); GO_BP:GO:0006313, transposition,DNA-mediated# (2e−66) 501 Tubulin beta chain, putative n = 1 Tax =Ricinus communis RepID = B9SB77_RICCO (2e−34); GO_MF:GO:0005525, GTPbinding# (2e−34); 8 70.6 77234145 77234873 GO_BP:GO:0051258, proteinpolymerization# (2e−34); GO_CC:GO:0043234, protein complex# (2e−34) 502Serine hydroxymethyltransferase n = 5 Tax = Poaceae RepID = Q7Y1F0_ORYSJ(2e−94); SHMT: Serine hydroxymethyltransferase (2.4e−30); Tubulin: 870.6 77234886 77237194 Tubulin/FtsZ family, GTPase domain (6.3e−06);GO_MF:GO:0030170, pyridoxal phosphate binding# (2e−94);GO_BP:GO:0006730, one-carbon metabolic process# (2e−94);GO_CC:GO:0048046, IDA#apoplast# (2e−89) 503 Catalytic/proteinphosphatase type 2C n = 1 Tax = Zea mays RepID = B6U289_MAIZE (9e−52);GO_MF:GO:0003824, catalytic activity# (9e−52); 8 70.7 72381917 72382396GO_BP:GO:0004721, phosphoprotein phosphatase activity# (4e−38);GO_CC:GO:0005886, plasma membrane# (1e−19) 504 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = Q6J9V9_MAIZE (1e−21) 8 70.774850214 74850548 505 VIP2 protein n = 1 Tax = Avena fatua RepID =Q9M4C5_AVEFA (1e−101); GO_MF:GO:0046872, metal ion binding# (1e−101) 870.7 82851656 82853989 506 Chaperone protein dnaJ, putative n = 1 Tax =Ricinus communis RepID = B9RNG7_RICCO (1e−142); DnaJ: DnaJ domain(1.2e−35); DnaJ_C: DnaJ C terminal 8 70.8 79424401 79433056 region(4.3e−18); GO_MF:GO:0051082, unfolded protein binding# (1e−145);GO_BP:GO:0006457, protein folding# (1e−145); GO_CC:GO:0005886, plasmamembrane# (1e−138) 507 OSJNBa0065O17.7 protein n = 1 Tax = Oryza sativaJaponica Group RepID = Q7XPS4_ORYSJ (6e−39); GO_MF:GO:0003964,RNA-directed DNA polymerase, 8 70.85 76769570 76770143 group II intronencoded# (6e−39); GO_BP:GO:0015074, DNA integration# (6e−39);GO_CC:GO:0005634, nucleus# (9e−34) 508 MAPK activating protein-like n =5 Tax = Oryza sativa RepID = Q5N8F0_ORYSJ (8e−25); DUF292: Eukaryoticprotein of unknown function, DUF292 (0.0041); 8 70.9 76754358 76755616GO_MF:GO:0005524, ATP binding# (2e−12); GO_BP:GO:0006438, valyl-tRNAaminoacylation# (2e−12); GO_CC:GO:0005737, cytoplasm# (2e−12) 509OSJNBa0065O17.7 protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q7XPS4_ORYSJ (8e−25); GO_MF:GO:0003964, RNA-directed DNA polymerase, 870.9 76766268 76769413 group II intron encoded# (8e−25);GO_BP:GO:0015074, DNA integration# (8e−25) 510 Protein SEY1, putative n= 1 Tax = Ricinus communis RepID = B9RQ05_RICCO (9e−72); RHD3: Root hairdefective 3 GTP-binding protein (RHD3) (0.013); 8 70.9 83123484 83125993GO_MF:GO:0016787, hydrolase activity# (1e−101); GO_CC:GO:0016021,integral to membrane# (1e−101) 511 Serine/threonine-protein phosphatasen = 2 Tax = Andropogoneae RepID = C5Z0J0_SORBI (1e−175); Metallophos:Calcineurin-like phosphoesterase (7.6e−45); 8 70.95 83126356 83130765GO_MF:GO:0016787, hydrolase activity# (1e−161); GO_BP:GO:0004721,phosphoprotein phosphatase activity# (1e−161); GO_CC:GO:0016459, myosincomplex# (1e−127) 512 Triosephosphate isomerase n = 1 Tax = Zea maysRepID = B6SMV7_MAIZE (1e−67); TIM: Triosephosphate isomerase (1.6e−33);ABC_membrane_2: 8 71 82634448 82642435 ABC transporter transmembraneregion (0.025); GO_MF:GO:0016853, isomerase activity# (1e−67);GO_BP:GO:0008152, metabolic process# (1e−67); GO_CC:GO:0005737,cytoplasm# (9e−60) 513 AT hook motif-containing protein, putative n = 2Tax = Oryza sativa Japonica Group RepID = Q2R0Z1_ORYSJ (0.0); CXC:Tesmin/TSO1-like CXC 8 71 83131432 83138464 domain (0.0019);GO_MF:GO:0004386, helicase activity# (0.0) 514 AT hook motif-containingprotein, putative n = 2 Tax = Oryza sativa Japonica Group RepID =Q2R0Z1_ORYSJ (1e−109); DUF889: Eukaryotic protein of 8 71.05 8313862183139769 unknown function (DUF889) (5.9e−09); GO_MF:GO:0004386, helicaseactivity# (1e−109) 515 SIN3 component, histone deacetylase complex n = 1Tax = Populus trichocarpa RepID = B9HLV3_POPTR (5e−18);GO_MF:GO:0016564, transcription repressor 8 71.1 76691823 76693893activity# (2e−18); GO_BP:GO:0006355, regulation of transcription,DNA-dependent# (6e−57); GO_CC:GO:0005634, nucleus# (6e−57) 516 Clathrinheavy chain, putative; 28833-19741 n = 14 Tax = Magnoliophyta RepID =Q9SRM1_ARATH (9e−60); ATP-synt_ab: ATP synthase alpha/beta family, 871.1 82584615 82587189 nucleotide-binding domain (0.064); Clathrin:Region in Clathrin and VPS (9.8e−30); GO_MF:GO:0005515, protein binding#(3e−64); GO_BP:GO:0016192, vesicle-mediated transport# (3e−64);GO_CC:GO:0030132, clathrin coat of coated pit# (3e−64) 517Galactosyltransferase family n = 3 Tax = Andropogoneae RepID =B6SXL2_MAIZE (3e−44); GO_MF:GO:0016757, transferase activity,transferring glycosyl 8 71.1 82619337 82620998 groups# (3e−44);GO_BP:GO:0006486, protein amino acid glycosylation# (3e−44);GO_CC:GO:0016021, integral to membrane# (3e−44) 518 OSIGBa0135L04.1protein n = 1 Tax = Oryza sativa RepID = Q01M88_ORYSA (4e−97);GO_MF:GO:0004386, helicase activity# (6e−74) 8 71.1 83140523 83144235519 Putative uncharacterized protein Sb09g030240 n = 2 Tax = Sorghumbicolor RepID = C5YWM7_SORBI (8e−72) 8 71.2 76647177 76648354 520 SIN3component, histone deacetylase complex n = 1 Tax = Populus trichocarpaRepID = B9HU88_POPTR (2e−10); GO_BP:GO:0006355, regulation of 8 71.276656395 76657117 transcription, DNA- dependent# (1e−17);GO_CC:GO:0005634, nucleus# (1e−17) 521 ATP binding protein n = 1 Tax =Zea mays RepID = B6U0Y9_MAIZE (8e−40); GO_MF:GO:0016597, amino acidbinding# (8e−40); 8 71.2 79379493 79380654 GO_BP:GO:0008152, metabolicprocess# (8e−40); GO_CC:GO:0005829, IDA#cytosol# (2e−20) 522 UvrB/uvrCmotif family protein n = 3 Tax = Andropogoneae RepID = B6TZ52_MAIZE(1e−149); UVR: UvrB/uvrC motif (0.011); UVR: UvrB/uvrC motif 8 71.2580438068 80441429 (0.02); DUF525: Protein of unknown function (DUF525)(6.7e−46); GO_MF:GO:0004518, nuclease activity# (1e−149);GO_BP:GO:0006289, IGI#nucleotide-excision repair# (1e−149) 523Cytochrome c oxidase polypeptide Vb n = 1 Tax = Zea mays RepID =B6U4H2_MAIZE (3e−52); COX5B: Cytochrome c oxidase subunit Vb (1e−16); 871.3 76641925 76644716 zf-CHCC: Zinc-finger domain (0.00083);GO_MF:GO:0004129, cbb3-type cytochrome c oxidase# (3e−52);GO_BP:GO:0004129, cbb3-type cytochrome c oxidase# (3e−52);GO_CC:GO:0005740, mitochondrial envelope# (3e−52) 524 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6TYK4_MAIZE(2e−69); GO_CC:GO:0009507, chloroplast# (3e−21) 8 71.3 82495083 82495878525 Putative uncharacterized protein Sb09g029710 n = 1 Tax = Sorghumbicolor RepID = C5YWG5_SORBI (9e−40); BSP: Plant Basic Secretory Protein(2.8e−35) 8 71.4 74660646 74662000 526 HAT family dimerisation domaincontaining protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QRD1_ORYSJ (3e−90); zf-BED: BED zinc finger 8 71.5 76561092 76562289(4.9e−06); GO_MF:GO:0046983, protein dimerization activity# (3e−90);GO_BP:GO:0006350, transcription# (9e−29) 527 LOB domain protein 25 n = 2Tax = Zea mays RepID = B6U340_MAIZE (3e−42); DUF260: Protein of unknownfunction DUF260 (2.1e−62); 8 71.5 82375676 82377525 GO_MF:GO:0005515,protein binding# (1e−41); GO_BP:GO:0010016, IMP#shoot morphogenesis#(1e−29); GO_CC:GO:0016020, membrane# (2e−42) 528 Putativeuncharacterized protein Sb09g004930 n = 2 Tax = Andropogoneae RepID =C5Z112_SORBI (4e−88); PIG-H: GPI-GlcNAc transferase complex, 8 71.582378172 82390011 PIG-H compon (2.3e−22); GO_MF:GO:0016788, hydrolaseactivity, acting on ester bonds# (1e−38) 529 Lysophospholipase 2-like n= 3 Tax = Oryza sativa RepID = Q5ZBI5_ORYSJ (1e−100); Abhydrolase_2:Phospholipase/Carboxylesterase (1.5e−37); 8 71.65 76522603 76529857GO_MF:GO:0016787, hydrolase activity# (1e−107) 530 Cyclin type B-like n= 1 Tax = Zea mays RepID = O24584_MAIZE (4e−15); GO_CC:GO:0005634,nucleus# (4e−15) 8 71.7 76501653 76501981 531 Peptide chain releasefactor 2 n = 1 Tax = Zea mays RepID = B6UHD9_MAIZE (4e−74); PCRF: PCRFdomain (1.4e−08); RF-1: Peptidyl-tRNA hydrolase 8 71.7 76502731 76505082domain (2.4e−05); GO_MF:GO:0016149, translation release factor activity,codon specific# (2e−79); GO_BP:GO:0016149, translation release factoractivity, codon specific# (2e−79); GO_CC:GO:0005737, cytoplasm# (2e−79)532 Zinc finger protein 7 n = 1 Tax = Zea mays RepID = B6U599_MAIZE(2e−41); GO_MF:GO:0008270, zinc ion binding# (2e−41); GO_CC:GO:0005622,8 71.7 76509380 76510008 intracellular# (2e−41) 533 Putativeuncharacterized protein Sb09g030180 n = 1 Tax = Sorghum bicolor RepID =C5YWM2_SORBI (2e−13) 8 71.7 76516695 76517305 534 Putativehomeobox-leucine zipper protein HOX26 n = 2 Tax = Oryza sativa RepID =HOX26_ORYSJ (7e−15); Homeobox: Homeobox domain (1.2e−05); 8 71.780678089 80680549 GO_MF:GO:0043565, sequence-specific DNA binding#(2e−84); GO_BP:GO:0045449, regulation of transcription# (2e−84);GO_CC:GO:0016021, integral to membrane# (2e−84) 535 Putativeribulose-1,5 bisphosphate carboxylase/oxygenase large subunitN-methyltransferase, chloroplast n = 1 Tax = Oryza sativa Japonica GroupRepID = 8 71.75 76493297 76498315 Q6ESK6_ORYSJ (2e−09); SET: SET domain(4.5e−08); Rubis-subs-bind: Rubisco LSMT substrate-binding (0.0073);GO_MF:GO:0016740, transferase activity# (2e−21); GO_CC:GO:0009507,chloroplast# (2e−09) 536 Plasminogen activator inhibitor 1 RNA-bindingprotein n = 2 Tax = Andropogoneae RepID = B6T4Q3_MAIZE (1e−102); GRP:Glycine rich protein family (0.088); 8 71.8 77429944 77433247 Stm1_N:Stm1 (0.024); HABP4_PAI-RBP1: Hyaluronan/mRNA binding family (7.3e−41);GO_CC:GO:0005737, cytoplasm# (6e−23) 537 Putative ubiquitin n = 1 Tax =Oryza sativa Japonica Group RepID = Q6L557_ORYSJ (1e−177); ubiquitin:Ubiquitin family (1.1e−12); ubiquitin: Ubiquitin 8 71.8 7743234877435177 family (0.00026); PI3_PI4_kinase: Phosphatidylinositol 3- and4-kinase (6.6e−80); GO_MF:GO:0016773, phosphotransferase activity,alcohol group as acceptor# (0.0); GO_BP:GO:0016301, kinase activity#(1e−149); GO_CC:GO:0005777, IDA#peroxisome# (1e−149) 538 Dihydrolipoyldehydrogenase n = 4 Tax = Poaceae RepID = C5Z0L0_SORBI (0.0);HI0933_like: HI0933-like protein (0.056); DAO: FAD dependent 8 71.8579300397 79307530 oxidoreductase (0.0028); FAD_binding_2: FAD bindingdomain (0.0017); GIDA: Glucose inhibited division protein A (0.002);Pyr_redox_2: Pyridine nucleotide-disulphide oxidored (6.3e−48);Pyr_redox: Pyridine nucleotide-disulphide oxidore (4.2e−23);Pyr_redox_dim: Pyridine nucleotide-disulphide oxidore (1.8e−41);GO_MF:GO:0050660, FAD binding# (0.0); GO_BP:GO:0055114, oxidationreduction# (0.0); GO_CC:GO:0043234, protein complex# (0.0) 539 60Sacidic ribosomal protein P2A n = 11 Tax = Andropogoneae RepID =RLA2A_MAIZE (3e−24); Ribosomal_60s: 60s Acidic ribosomal protein(2.2e−38); 8 71.9 73686297 73688554 GO_MF:GO:0003735, structuralconstituent of ribosome# (3e−24); GO_BP:GO:0006414, translationalelongation# (3e−24); GO_CC:GO:0030529, ribonucleoprotein complex#(3e−24) 540 Catalytic, putative n = 1 Tax = Ricinus communis RepID =B9RL97_RICCO (1e−125); Peptidase_S9: Prolyl oligopeptidase family(0.07); Abhydrolase_3: alpha/beta 8 71.9 73689972 73694162 hydrolasefold (1.3e−05); GO_MF:GO:0016787, hydrolase activity# (1e−130);GO_CC:GO:0016020, membrane# (1e−130) 541 H0702G05.5 protein n = 1 Tax =Oryza sativa RepID = Q25AI8_ORYSA (1e−41); cwf21: cwf21 (3.2e−22);GO_MF:GO:0003677, DNA binding# (2e−13) 8 71.9 73724531 73725644 542 ATPbinding protein n = 1 Tax = Zea mays RepID = B6U656_MAIZE (1e−170);Pkinase: Protein kinase domain (2.7e−38); Pkinase_Tyr: Protein tyrosinekinase 8 71.9 73726676 73730357 (5.8e−32); GO_MF:GO:0005524, ATPbinding# (1e−170); GO_BP:GO:0006468, protein amino acid phosphorylation#(1e−170); GO_CC:GO:0005886, plasma membrane# (1e−113) 543 Putativeuncharacterized protein Sb09g029450 n = 2 Tax = Andropogoneae RepID =C5YW44_SORBI (1e−175) 8 71.9 73731498 73738039 544Dihydroflavonol-4-reductase n = 3 Tax = Andropogoneae RepID =B6TK03_MAIZE (1e−167); adh_short: short chain dehydrogenase (0.0004);Epimerase: 8 71.9 73846714 73852084 NAD dependent epimerase/dehydratasefamily (1.3e−05); 3Beta_HSD: 3-beta hydroxysteroiddehydrogenase/isomerase family (2.3e−06); NAD_binding_4: Male sterilityprotein (0.04); GO_MF:GO:0005488, binding# (1e−167); GO_BP:GO:0008152,metabolic process# (1e−167) 545 Putative polygalacturonase n = 1 Tax =Oryza sativa Japonica Group RepID = Q6L5E7_ORYSJ (1e−155);Glyco_hydro_28: Glycosyl hydrolases family 28 (1e−87); 8 71.9 7385106473854409 GO_MF:GO:0016798, hydrolase activity, acting on glycosyl bonds#(0.0); GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0005618,IDA#cell wall# (4e−79) 546 Ribonuclease P n = 3 Tax = AndropogoneaeRepID = B6T7F1_MAIZE (5e−25); Prefoldin_2: Prefoldin subunit (0.0025);GO_MF:GO:0003676, nucleic acid 8 71.9 73872086 73875838 binding#(5e−25); GO_BP:GO:0006508, proteolysis# (2e−24); GO_CC:GO:0016272,prefoldin complex# (4e−22) 547 Calmodulin binding protein n = 2 Tax =Andropogoneae RepID = B6U170_MAIZE (2e−96); GO_MF:GO:0005516,IDA#calmodulin binding# (2e−11) 8 71.9 73875938 73878129 548 PnFL-2 n =3 Tax = Andropogoneae RepID = B6T7Q6_MAIZE (2e−40) 8 71.9 7388127373881812 549 GATA transcription factor 19 n = 1 Tax = Zea mays RepID =B6TS85_MAIZE (2e−32); GATA: GATA zinc finger (1.6e−19);GO_MF:GO:0046872, metal ion 8 71.9 73894507 73895669 binding# (2e−32);GO_BP:GO:0006355, regulation of transcription, DNA-dependent# (2e−32);GO_CC:GO:0005634, nucleus# (1e−14) 550 Sugar transporter protein n = 1Tax = Ananas comosus RepID = A4GXC8_ANACO (0.0); Sugar_tr: Sugar (andother) transporter (3.9e−104); MFS_1: 8 71.9 73900392 73906519 MajorFacilitator Superfamily (5.9e−13); GO_MF:GO:0022891, substrate-specifictransmembrane transporter activity# (0.0); GO_BP:GO:0055085,transmembrane transport# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 551 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =C4J377_MAIZE (4e−09) 8 71.9 74125695 74125928 552 Potassium transporter10 n = 2 Tax = Andropogoneae RepID = B6SS13_MAIZE (3e−79); K_trans: K+potassium transporter (2e−09); GO_MF:GO:0015079, 8 71.9 7412972074130514 potassium ion transmembrane transporter activity# (3e−79);GO_BP:GO:0015079, potassium ion transmembrane transporter activity#(3e−79); GO_CC:GO:0016020, membrane# (3e−79) 553 10A19I.4 n = 3 Tax =Oryza sativa Japonica Group RepID = Q9XHW2_ORYSJ (1e−107); HhH-GPD:HhH-GPD superfamily base excision DNA repair protein 8 71.9 7413179274133362 (3.9e−22); GO_MF:GO:0003824, catalytic activity# (1e−136);GO_BP:GO:0006284, base-excision repair# (1e−136); GO_CC:GO:0005634,nucleus# (2e−81) 554 Pentatricopeptide repeat-containing protein,putative n = 1 Tax = Ricinus communis RepID = B9S8W1_RICCO (1e−117);PPR: PPR repeat (0.15); TPR_4: 8 71.9 74249467 74251973Tetratricopeptide repeat (0.12); TPR_4: Tetratricopeptide repeat (4.2);PPR: PPR repeat (7.8e−05); TPR_4: Tetratricopeptide repeat (0.2); TPR_4:Tetratricopeptide repeat (14); PPR: PPR repeat (0.018); TPR_4:Tetratricopeptide repeat (18); PPR: PPR repeat (4.1e−07); PPR: PPRrepeat (3.6e−11); PPR: PPR repeat (3.7); PPR: PPR repeat (2.4); TPR_4:Tetratricopeptide repeat (25); GO_MF:GO:0005488, binding# (0.0) 555 Ulp1protease family, C-terminal catalytic domain containing protein n = 2Tax = Oryza sativa Japonica Group RepID = Q109R5_ORYSJ (2e−19); 8 71.974301564 74303726 GO_MF:GO:0008234, cysteine-type peptidase activity#(9e−67); GO_BP:GO:0006508, proteolysis# (9e−67) 556 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C0PMZ8_MAIZE(2e−34); GO_MF:GO:0008234, cysteine-type peptidase activity# (2e−34); 871.9 74304167 74304498 GO_BP:GO:0006508, proteolysis# (2e−34) 557RuBisCo subunit binding-protein beta subunit (Fragment) n = 1 Tax = Zeamays RepID = Q6B7Q9_MAIZE (5e−62); GO_MF:GO:0005524, ATP binding#(5e−60); 8 71.9 74307088 74308237 GO_BP:GO:0044267, cellular proteinmetabolic process# (5e−60); GO_CC:GO:0009536, plastid# (5e−60) 558 DNAhelicase homolog, putative n = 1 Tax = Musa acuminata RepID =Q1EPC6_MUSAC (2e−93); DUF889: Eukaryotic protein of unknown function(DUF889) 8 71.9 74311462 74312484 (6.5e−15); GO_MF:GO:0004386, helicaseactivity# (2e−93) 559 DNA helicase homolog, putative n = 1 Tax = Musaacuminata RepID = Q1EPC6_MUSAC (4e−10); GO_MF:GO:0004386, helicaseactivity# (4e−10) 8 71.9 74315968 74320576 560 UPF0737 protein 1 n = 1Tax = Zea mays RepID = U7371_MAIZE (1e−36); GO_CC:GO:0005634, nucleus#(1e−36) 8 71.9 74320992 74321396 561 Late-embryogenesis-abundant proteinn = 4 Tax = Andropogoneae RepID = C7E3V1_SACOF (2e−85); LEA_2: Lateembryogenesis abundant protein (6.6e−90); 8 71.9 74938506 74939595GO_BP:GO:0009269, response to desiccation# (1e−73); GO_CC:GO:0005886,plasma membrane# (5e−49) 562 Putative uncharacterized protein n = 1 Tax= Zea mays RepID = B6U8E7_MAIZE (8e−50) 8 71.9 74989358 74990328 563BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, putative n =1 Tax = Ricinus communis RepID = B9T3S1_RICCO (1e−26); 8 71.9 7500426775006457 GO_MF:GO:0005524, ATP binding# (5e−32); GO_BP:GO:0006468,protein amino acid phosphorylation# (5e−32); GO_CC:GO:0016021, integralto membrane# (1e−26) 564 ATP binding protein, putative n = 1 Tax =Ricinus communis RepID = B9RIQ4_RICCO (0.0); AAA_3: ATPase familyassociated with various (0.0048); 8 71.9 75007473 75012890 AAA: ATPasefamily associated with various cellular activities (AAA) (2.5e−78);AAA_5: ATPase family associated with various (0.0016); GO_MF:GO:0017111,nucleoside-triphosphatase activity# (0.0); GO_BP:GO:0051301, celldivision# (0.0) 565 Putative uncharacterized protein Sb01g044750 n = 1Tax = Sorghum bicolor RepID = C5WUV8_SORBI (2e−32); GO_MF:GO:0046983,protein dimerization 8 71.9 82154905 82155374 activity# (8e−10);GO_BP:GO:0006355, regulation of transcription, DNA-dependent# (8e−10);GO_CC:GO:0005634, nucleus# (8e−10) 566 CCT motif family protein n = 2Tax = Zea mays RepID = B6U5M8_MAIZE (1e−114) 8 71.95 79070986 79074301567 Hexose transporter (Fragment) n = 5 Tax = Zea mays RepID =Q9LLD9_MAIZE (3e−10); GO_MF:GO:0022891, substrate-specific transmembranetransporter 8 72 73478314 73479582 activity# (3e−10); GO_BP:GO:0055085,transmembrane transport# (3e−10); GO_CC:GO:0016021, integral tomembrane# (3e−10) 568 Latex-abundant protein n = 2 Tax = AndropogoneaeRepID = B4FNB7_MAIZE (1e−17); GO_MF:GO:0004197, cysteine-typeendopeptidase activity# (1e−27); 8 72 73520240 73520491GO_BP:GO:0006508, proteolysis# (1e−27) 569 Putative uncharacterizedprotein Sb09g029410 n = 1 Tax = Sorghum bicolor RepID = C5YW40_SORBI(7e−66); DUF617: Protein of unknown function, DUF617 8 72 7363699173637806 (2.2e−15) 570 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = C0PAB7_MAIZE (2e−19) 8 72 75282999 75292186 571 Tubulingamma-1 chain n = 28 Tax = Embryophyta RepID = TBG1_ARATH (0.0);Tubulin: Tubulin/FtsZ family, GTPase domain (8e−94); Tubulin_C: Tubulin/8 72.05 79279716 79285737 FtsZ family, C-terminal domain (2.6e−59);GO_MF:GO:0005525, GTP 708cellular localization# (0.0); GO_CC:GO:0043234,protein complex# (0.0) 572 Inorganic phosphate transporter 1-7 n = 3 Tax= Andropogoneae RepID = B6TXX9_MAIZE (8e−61); GO_MF:GO:0005315,inorganic phosphate 8 72.05 80685551 80686059 transmembrane transporteractivity# (8e−61); GO_BP:GO:0006817, phosphate transport# (8e−61);GO_CC:GO:0016021, integral to membrane# (8e−61) 573 Zeonl gag protein n= 3 Tax = Zea mays RepID = Q7XBD3_MAIZE (8e−19) 8 72.1 82026765 82028638574 Putative uncharacterized protein Sb09g004280 n = 2 Tax =Andropogoneae RepID = C5Z0J2_SORBI (8e−44) 8 72.1 83245239 83247221 575Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B4F8F2_MAIZE (1e−77); GO_MF:GO:0003676, nucleic acid binding# (4e−35) 872.3 76316289 76317707 576 Probable protein phosphatase 2C 52 n = 2 Tax= Oryza sativa RepID = P2C52_ORYSJ (2e−91); PP2C: Protein phosphatase 2C(8.4e−46); GO_MF:GO:0046872, 8 72.3 76319202 76324609 metal ion binding#(1e−96); GO_BP:GO:0006470, protein amino acid dephosphorylation#(1e−96); GO_CC:GO:0008287, protein serine/threonine phosphatase complex#(1e−96) 577 Probable protein phosphatase 2C 52 n = 2 Tax = Oryza sativaRepID = P2C52_ORYSJ (3e−20); GO_MF:GO:0046872, metal ion binding#(5e−75); 8 72.3 76331266 76331972 GO_BP:GO:0006470, protein amino aciddephosphorylation# (5e−75); GO_CC:GO:0008287, protein serine/threoninephosphatase complex# (5e−75) 578 NADH-ubiquinone oxidoreductase 10.5 kDasubunit n = 4 Tax = Andropogoneae RepID = B6TDN0_MAIZE (6e−31);L51_S25_CI-B8: Mitochondrial ribosomal 8 72.35 80681445 80685001 proteinL51/S25/CI-B8 domain (1.6e−10); GO_MF:GO:0016491, oxidoreductaseactivity# (1e−17); GO_BP:GO:0055114, oxidation reduction# (1e−17);GO_CC:GO:0045271, IDA#respiratory chain complex I# (4e−23) 579 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6TGL0_MAIZE(3e−75) 8 72.4 81880838 81881973 580 Putative seryl-tRNA synthetase n =1 Tax = Oryza sativa Japonica Group RepID = Q5JKQ2_ORYSJ (1e−41);Seryl_tRNA_N: Seryl-tRNA synthetase 8 72.55 74456288 74459537 N-terminaldomain (0.0053); GO_MF:GO:0016874, ligase activity# (1e−49);GO_BP:GO:0006434, seryl-tRNA aminoacylation# (1e−49); GO_CC:GO:0005737,cytoplasm# (1e−49) 581 BC10 protein n = 2 Tax = Oryza sativa RepID =Q65XS5_ORYSJ (2e−60); DUF266: Arabidopsis protein of unknown function,DUF266 (4.2e−18); 8 72.65 80791555 80798628 GO_MF:GO:0008375,acetylglucosaminyltransferase activity# (7e−68); GO_CC:GO:0016020,membrane# (7e−68) 582 Probable protein ABIL4 n = 3 Tax = Oryza sativaRepID = ABIL4_ORYSJ (2e−80); GO_MF:GO:0005515, protein binding# (2e−37);GO_BP:GO:0045010, 8 72.7 75598612 75601628 PMID: 11559594#actinnucleation# (2e−33); GO_CC:GO:0005856, cytoskeleton# (2e−80) 583Putative calcium-dependent protein kinase n = 1 Tax = Oryza sativaJaponica Group RepID = Q5ZE73_ORYSJ (0.0); Kdo: Lipopolysaccharidekinase (Kdo) 8 72.7 75601806 75605555 (0.082); Pkinase: Protein kinasedomain (8.6e−103); Pkinase_Tyr: Protein tyrosine kinase (5.2e−11);efhand: EF hand (2.8e−07); efhand: EF hand (5.7e−06); efhand: EF hand(1.1e−06); efhand: EF hand (8.3e−09); GO_MF:GO:0016740, transferaseactivity# (0.0); GO_BP:GO:0016301, kinase activity# (0.0);GO_CC:GO:0005886, plasma membrane# (0.0) 584 OSJNBa0039G19.7 protein n =1 Tax = Oryza sativa Japonica Group RepID = Q7XXD1_ORYSJ (1e−166); PPR:PPR repeat (6.7e−06); PPR: PPR repeat (2.6); PPR: 8 72.7 7758839877590631 PPR repeat (6.5); GO_MF:GO:0005488, binding# (8e−89);GO_BP:GO:0006306, DNA methylation# (8e−89); GO_CC:GO:0005739,mitochondrion# (6e−72) 585 Dihydrolipoyl dehydrogenase n = 4 Tax =Poaceae RepID = C5Z0L0_SORBI (0.0); HI0933_like: HI0933-like protein(0.056); DAO: FAD dependent 8 72.7 79203447 79210580 oxidoreductase(0.0028); FAD_binding_2: FAD binding domain (0.0017); GIDA: Glucoseinhibited division protein A (0.002); Pyr_redox_2: Pyridinenucleotide-disulphide oxidored (6.3e−48); Pyr_redox: Pyridinenucleotide-disulphide oxidore (4.2e−23); Pyr_redox_dim: Pyridinenucleotide-disulphide oxidore (1.8e−41); GO_MF:GO:0050660, FAD binding#(0.0); GO_BP:GO:0055114, oxidation reduction# (0.0); GO_CC:GO:0043234,protein complex# (0.0) 586 OSJNBa0070M12.5 protein n = 2 Tax = Oryzasativa RepID = Q7XTM0_ORYSJ (6e−25); GO_MF:GO:0046872, metal ionbinding# (6e−25) 8 72.7 80934925 80936751 587 Disease resistanceprotein-like n = 2 Tax = Oryza sativa RepID = Q9AXB2_ORYSJ (1e−143);GO_MF:GO:0043565, sequence-specific DNA binding# (2e−59); 8 72.7575641962 75645944 GO_BP:GO:0045449, regulation of transcription#(2e−59); GO_CC:GO:0031224, intrinsic to membrane# (2e−59) 588Serine-threonine protein kinase, putative n = 1 Tax = Ricinus communisRepID = B9RCP0_RICCO (1e−136); DUF1221: Protein of unknown function 872.8 75648367 75650800 (DUF1221) (4.1e−108); Pkinase: Protein kinasedomain (5.4e−22); Pkinase_Tyr: Protein tyrosine kinase (6.3e−17);GO_MF:GO:0005524, ATP binding# (0.0); GO_BP:GO:0006468, protein aminoacid phosphorylation# (0.0); GO_CC:GO:0016020, membrane# (6e−26) 589BC10 protein n = 2 Tax = Oryza sativa RepID = Q65XS5_ORYSJ (2e−89);DUF266: Arabidopsis protein of unknown function, DUF266 (3e−19); 8 72.8580822276 80825863 GO_MF:GO:0008375, acetylglucosaminyltransferaseactivity# (2e−89); GO_CC:GO:0016020, membrane# (2e−89) 590Polygalacturonase n = 1 Tax = Zea mays RepID = B6TDS0_MAIZE (0.0);Glyco_hydro_28: Glycosyl hydrolases family 28 (1.7e−10); Pec_lyase_C: 872.9 76128003 76130303 Pectate lyase (0.078); GO_MF:GO:0016798,hydrolase activity, acting on glycosyl bonds# (0.0); GO_BP:GO:0008152,metabolic process# (0.0); GO_CC:GO:0005773, IDA#vacuole# (1e−111) 591Gag-pol n = 1 Tax = Zea mays RepID = Q8W1D1_MAIZE (3e−51);GO_MF:GO:0008270, zinc ion binding# (3e−51); GO_BP:GO:0015074, DNA 872.9 79636242 79636718 integration# (3e−51); GO_CC:GO:0005634, nucleus#(3e−51) 592 Gag-pol n = 1 Tax = Zea mays RepID = Q8W1D1_MAIZE (6e−18);GO_MF:GO:0008270, zinc ion binding# (6e−18); GO_BP:GO:0015074, DNA 872.9 79636906 79637138 integration# (6e−18); GO_CC:GO:0005634, nucleus#(6e−18) 593 Ribosomal protein L18 n = 16 Tax = Poaceae RepID =Q5WMY3_ORYSJ (3e−85); Ribosomal_L18e: Eukaryotic ribosomal protein L18(2.3e−103); 8 72.9 83335693 83339014 GO_MF:GO:0003735, structuralconstituent of ribosome# (3e−85); GO_BP:GO:0006412, translation#(3e−85); GO_CC:GO:0030529, ribonucleoprotein complex# (3e−85) 594Probable cellulose synthase A catalytic subunit 1 [UDP-forming] n = 15Tax = Poaceae RepID = CESA1_ORYSJ (0.0); PHD: PHD-finger (0.015);zf-C3HC4: Zinc 8 73 80220199 80226474 finger, C3HC4 type (RING finger)(0.094); Cellulose_synt: Cellulose synthase (0); GO_MF:GO:0046872, metalion binding# (0.0); GO_BP:GO:0030244, cellulose biosynthetic process#(0.0); GO_CC:GO:0016021, integral to membrane# (0.0) 595 Putativeuncharacterized protein n = 2 Tax = Zea mays RepID = C0PM59_MAIZE(1e−127) 8 73.05 79163688 79167287 596 Mitochondrial carrier-likeprotein n = 1 Tax = Solanum tuberosum RepID = Q2PYY0_SOLTU (7e−19);Mito_carr: Mitochondrial carrier protein (6.3e−05); 8 73.1 7582174375822198 GO_MF:GO:0030528, transcription regulator activity# (3e−24);GO_BP:GO:0055085, transmembrane transport# (3e−24); GO_CC:GO:0016021,integral to membrane# (3e−24) 597 Extra-large G-protein-like n = 2 Tax =Oryza sativa RepID = Q6K2T0_ORYSJ (2e−61); GO_CC:GO:0005886, plasmamembrane# (5e−56) 8 73.2 76036061 76039111 598 DNA binding protein n = 1Tax = Zea mays RepID = B6TXV7_MAIZE (2e−89); HLH: Helix-loop-helixDNA-binding domain (4.6e−11); 8 73.2 79141710 79142884 GO_MF:GO:0030528,transcription regulator activity# (2e−89); GO_BP:GO:0045449, regulationof transcription# (2e−89); GO_CC:GO:0005634, nucleus# (2e−89) 599 Ringfinger protein, putative n = 1 Tax = Ricinus communis RepID =B9RS17_RICCO (3e−28); PHD: PHD-finger (0.05); zf-C3HC4: Zinc finger,C3HC4 type 8 73.2 79149486 79150526 (RING finger) (2.2e−07);GO_MF:GO:0046872, metal ion binding# (7e−57); GO_BP:GO:0004842,NAS#ubiquitin-protein ligase activity# (2e−28); GO_CC:GO:0016021,integral to membrane# (9e−30) 600 Putative uncharacterized proteinorf105-e n = 1 Tax = Zea mays RepID = Q6R991_MAIZE (2e−18);GO_CC:GO:0005739, mitochondrion# (2e−18) 8 73.2 80827284 80827898 601Putative uncharacterized protein n = 2 Tax = Oryza sativa RepID =Q851R1_ORYSJ (2e−19) 8 73.2 81415796 81417430 602 Catalytic/proteinphosphatase type 2C n = 4 Tax = Andropogoneae RepID = B6TT19_MAIZE(7e−10); GO_MF:GO:0003824, catalytic activity# (6e−10) 8 73.25 7993441379935030 603 C4-dicarboxylate transporter-like protein n = 2 Tax = Oryzasativa RepID = Q8L4G8_ORYSJ (1e−131); C4dic_mal_tran: C4-dicarboxylatetransporter/malic 8 73.3 75414377 75470871 acid transport protein(8.9e−63); GO_MF:GO:0046872, metal ion binding# (4e−26);GO_BP:GO:0055085, transmembrane transport# (1e−177); GO_CC:GO:0016021,integral to membrane# (1e−177) 604 HAT family dimerisation domaincontaining protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q53M48_ORYSJ (5e−71); GO_MF:GO:0046983, 8 73.3 75955890 75956803 proteindimerization activity# (5e−86) 605 Probable indole-3-acetic acid-amidosynthetase GH3.5 n = 3 Tax = Oryza sativa RepID = GH35_ORYSJ (0.0); GH3:GH3 auxin-responsive promoter (2e−284); 8 73.4 75985652 75990570tRNA_int_endo_N: tRNA intron endonuclease, N-terminal domain (0.096);GO_MF:GO:0016874, ligase activity# (0.0); GO_BP:GO:0009733, IEP#responseto auxin stimulus# (0.0); GO_CC:GO:0005773, IDA#vacuole# (0.0) 606 DNAbinding protein, putative n = 1 Tax = Ricinus communis RepID =B9RWI9_RICCO (9e−78); HLH: Helix-loop-helix DNA-binding domain(7.3e−15); 8 73.4 75990716 75992165 GO_MF:GO:0030528, transcriptionregulator activity# (1e−112); GO_BP:GO:0045449, regulation oftranscription# (1e−112); GO_CC:GO:0005634, nucleus# (1e−112) 607 Proteinphosphatase 2C ABI2 n = 2 Tax = Zea mays RepID = B6TN97_MAIZE (0.0);PP2C: Protein phosphatase 2C (1.7e−76); GO_MF:GO:0046872, 8 73.477983697 77986868 metal ion binding# (0.0); GO_BP:GO:0006470, proteinamino acid dephosphorylation# (0.0); GO_CC:GO:0008287, proteinserine/threonine phosphatase complex# (0.0) 608 Putative gag-polpolyprotein n = 1 Tax = Zea mays RepID = Q8SA91_MAIZE (4e−24);GO_MF:GO:0004190, penicillopepsin activity# (4e−24); 8 73.4 7803937878039764 GO_BP:GO:0015074, DNA integration# (4e−24); GO_CC:GO:0005634,nucleus# (4e−24) 609 Translation initiation factor n = 1 Tax = Pisumsativum RepID = Q8H6S8_PEA (0.0); GTP_EFTU: Elongation factor Tu GTPbinding domain (1.5e−42); 8 73.4 78073256 78079737 MMR_HSR1: GTPase ofunknown function (7.4e−05); GTP_EFTU_D2: Elongation factor Tu domain 2(8.5e−05); GO_MF:GO:0005525, GTP binding# (0.0); GO_BP:GO:0003743,protein-synthesizing GTPase activity, initiation# (0.0);GO_CC:GO:0005829, IDA#cytosol# (0.0) 610 ATP binding protein, putative n= 1 Tax = Ricinus communis RepID = B9SAN5_RICCO (2e−38); AAA: ATPasefamily associated with various cellular 8 73.4 78114912 78115537activities (AAA) (1.2e−05); GO_MF:GO:0017111, nucleoside-triphosphataseactivity# (4e−39); GO_BP:GO:0009229, thiamin diphosphate biosyntheticprocess# (3e−37) 611 DNA binding protein, putative n = 1 Tax = Ricinuscommunis RepID = B9S516_RICCO (1e−107); GO_MF:GO:0003677, DNA binding#(0.0); 8 73.4 78189173 78208974 GO_CC:GO:0005634, nucleus# (1e−107) 612Pepsin A n = 2 Tax = Zea mays RepID = B6SWN4_MAIZE (0.0); Asp:Eukaryotic aspartyl protease (0.038); GO_MF:GO:0004190, penicillopepsinactivity# (0.0); 8 73.4 79126285 79128196 GO_BP:GO:0006508, proteolysis#(0.0) 613 Boron transporter n = 5 Tax = Oryza sativa RepID =Q1ZYR7_ORYSJ (0.0); HCO3_cotransp: HCO3-transporter family (4.2e−16);BCCT: BCCT family 8 73.4 79936497 79940559 transporter (0.092);GO_MF:GO:0015380, anion exchanger activity# (0.0); GO_BP:GO:0015380,anion exchanger activity# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 614 Putative uncharacterized protein Sb09g005260 n = 1 Tax =Sorghum bicolor RepID = C5Z151_SORBI (3e−37); Alba: Alba (1.1e−20);GO_MF:GO:0003676, 8 73.4 80385418 80386690 nucleic acid binding# (6e−35)615 Nucleotide sugar translocator BT2A n = 4 Tax = Zea mays RepID =B2LWG5_MAIZE (0.0); Mito_carr: Mitochondrial carrier protein (1.7e−27);Mito_carr: 8 73.4 80386832 80390311 Mitochondrial carrier protein(3.3e−34); Mito_carr: Mitochondrial carrier protein (4.6e−33);GO_MF:GO:0005488, binding# (0.0); GO_BP:GO:0055085, transmembranetransport# (0.0); GO_CC:GO:0016021, integral to membrane# (0.0) 616ANAC075 n = 3 Tax = Andropogoneae RepID = B6UC12_MAIZE (2e−26); NAM: Noapical meristem (NAM) protein (1.2e−06); GO_MF:GO:0003677, 8 73.480621133 80624343 DNA binding# (1e−51); GO_BP:GO:0045449, regulation oftranscription# (1e−51); GO_CC:GO:0005634, nucleus# (1e−27) 617 Chaperoneclpb, putative n = 1 Tax = Ricinus communis RepID = B9SJA7_RICCO(6e−18); GO_MF:GO:0017111, nucleoside-triphosphatase activity# (2e−19);8 73.4 80627876 80629816 GO_BP:GO:0019538, protein metabolic process#(2e−19); GO_CC:GO:0009570, IDA#chloroplast stroma# (6e−18) 618 Putativeuncharacterized protein Sb03g023091 (Fragment) n = 1 Tax = Sorghumbicolor RepID = C5XM85_SORBI (1e−23) 8 73.4 80662391 80663581 619 AP2domain containing protein n = 1 Tax = Zea mays RepID = B6SQ62_MAIZE(1e−115); AP2: AP2 domain (2.9e−18); GO_MF:GO:0003700, transcription 873.4 80979506 80980774 factor activity# (1e−115); GO_BP:GO:0045449,regulation of transcription# (1e−115); GO_CC:GO:0005634, nucleus#(1e−115) 620 Endo-1,3;1,4-beta-D-glucanase n = 1 Tax = Zea mays RepID =B4FTK9_MAIZE (1e−134); DLH: Dienelactone hydrolase family (2.3e−13); 873.4 88889056 88893568 GO_MF:GO:0016787, hydrolase activity# (4e−77);GO_BP:GO:0009651, IEP#response to salt stress# (2e−72);GO_CC:GO:0048046, IDA#apoplast# (2e−72) 621 DNA binding protein,putative n = 1 Tax = Ricinus communis RepID = B9S516_RICCO (1e−130);HSA: HSA (1.4e−13); GO_MF:GO:0003677, 8 73.45 78209319 78257571 DNAbinding# (0.0); GO_CC:GO:0005634, nucleus# (1e−130) 622 GPI-anchoredprotein n = 2 Tax = Zea mays RepID = B6TM89_MAIZE (2e−33); X8: X8 domain(1.7e−48); GO_MF:GO:0016787, hydrolase activity# (6e−33); 8 73.574621022 74622720 GO_BP:GO:0008152, metabolic process# (3e−26) 623UV-damaged DNA binding protein n = 3 Tax = Oryza sativa RepID =Q9FS08_ORYSJ (9e−53); GO_MF:GO:0003676, nucleic acid binding# (9e−53); 873.5 77723776 77724872 GO_BP:GO:0016481, negative regulation oftranscription# (3e−50); GO_CC:GO:0005634, nucleus# (9e−53) 624 Putativevacuolar ATP synthase subunit C (Fragment) n = 1 Tax = Oryza sativaJaponica Group RepID = Q84PC1_ORYSJ (0.0); V-ATPase_C: V-ATPase 8 73.577746144 77752266 subunit C (1.3e−192); GO_MF:GO:0016820, hydrolaseactivity, acting on acid anhydrides, catalyzing transmembrane movementof substances# (0.0); GO_BP:GO:0016820, hydrolase activity, acting onacid anhydrides, catalyzing transmembrane movement of substances# (0.0);GO_CC:GO:0033180, proton-transporting V-type ATPase, V1 domain# (0.0)625 CCAAT-box-binding transcription factor-like protein n = 3 Tax =Oryza sativa RepID = Q6YU01_ORYSJ (6e−33); GO_MF:GO:0005488, binding#(8e−22); 8 73.5 79688507 79689087 GO_BP:GO:0010197, IMP#polar nucleusfusion# (3e−20); GO_CC:GO:0005730, IDA# nucleolus# (3e−20) 626 GDPdissociation inhibitor n = 7 Tax = Brassicaceae RepID = Q8LBY8_ARATH(4e−99); GDI: GDP dissociation inhibitor (8.8e−37); GO_MF:GO:0043087, 873.5 79689525 79691113 regulation of GTPaseVactivity# (1e−105);GO_BP:GO:0043087, regulation of GTPase activity# (1e−105);GO_CC:GO:0005737, cytoplasm# (1e−56) 627 Putative uncharacterizedprotein Sb09g004360 n = 4 Tax = Andropogoneae RepID = C5Z0K3_SORBI(1e−104) 8 73.5 79694403 79697709 628 Integral membrane protein like n =1 Tax = Zea mays RepID = B6SMU5_MAIZE (1e−127); Nuc_sug_transp:Nucleotide-sugar transporter (0.033); DUF6: 8 73.5 81159846 81164547Integral membrane protein DUF6 (0.04); TPT: Triose-phosphate Transporterfamily (6.1e−49); GO_BP:GO:0009624, IEP#response to nematode# (1e−36);GO_CC:GO:0016021, integral to membrane# (1e−110) 629 Leucine Rich Repeatfamily protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q2R0X3_ORYSJ (0.0); LRR_1: Leucine Rich Repeat (1.4); 8 73.5 8124420881248033 LRR_1: Leucine Rich Repeat (2.4); LRR_1: Leucine Rich Repeat(0.24); LRR_1: Leucine Rich Repeat (51); LRR_1: Leucine Rich Repeat(19); LRR_1: Leucine Rich Repeat (1.1); LRR_1: Leucine Rich Repeat (16);LRR_1: Leucine Rich Repeat (4.3); LRR_1: Leucine Rich Repeat (0.65);LRR_1: Leucine Rich Repeat (0.47); LRR_1: Leucine Rich Repeat (3.3);LRR_1: Leucine Rich Repeat (1.1); LRR_1: Leucine Rich Repeat (1.4);LRR_1: Leucine Rich Repeat (9.9); LRR_1: Leucine Rich Repeat (4.6);LRR_1: Leucine Rich Repeat (3.4); LRR_1: Leucine Rich Repeat (2.6);LRR_1: Leucine Rich Repeat (46); Pkinase: Protein kinase domain(3.9e−34); Pkinase_Tyr: Protein tyrosine kinase (1.3e−15);GO_MF:GO:0005524, ATP binding# (0.0); GO_BP:GO:0006468, protein aminoacid phosphorylation# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 630 HIRA-interacting protein, putative n = 1 Tax = Ricinuscommunis RepID = B9SG71_RICCO (3e−94); Nfu_N: Scaffold protein Nfu/NifUN terminal (2.3e−57); 8 73.5 83396432 83400437 NifU: NifU-like domain(5.2e−29); GO_MF:GO:0051536, iron-sulfur cluster binding# (1e−104);GO_BP:GO:0016226, iron-sulfur cluster assembly# (1e−104);GO_CC:GO:0005739, mitochondrion# (1e−92) 631 Importin subunit alpha-1b n= 5 Tax = Poaceae RepID = IMA1B_ORYSJ (0.0); IBB: Importin beta bindingdomain (4.6e−26); Arm: Armadillo/beta-catenin-like 8 73.5 8343594083441077 repeat (3.1); HEAT: HEAT repeat (26); Arm:Armadillo/beta-catenin-like repeat (5.1e−11); HEAT: HEAT repeat (5.1);Arm: Armadillo/beta-catenin-like repeat (7.9e−14); HEAT: HEAT repeat(0.002); Arm: Armadillo/beta-catenin-like repeat (2.5e−08); Arm:Armadillo/beta-catenin-like repeat (2.1e−06); HEAT: HEAT repeat (1.6);Arm: Armadillo/beta-catenin-like repeat (2.1e−10); HEAT: HEAT repeat(1.5); Arm: Armadillo/beta-catenin-like repeat (8e−11); HEAT: HEATrepeat (9.3); Arm: Armadillo/beta-catenin-like repeat (1.6e−13); HEAT:HEAT repeat (30); Arm: Armadillo/beta-catenin-like repeat (4.5e−07);HEAT: HEAT repeat (37); GO_MF:GO:0008565, protein transporter activity#(0.0); GO_BP:GO:0015031, protein transport# (0.0); GO_CC:GO:0048471,ISS#perinuclear region of cytoplasm# (0.0) 632 Eukaryotic translationinitiation factor 3 subunit (EIF-3)-like n = 3 Tax = Oryza sativa RepID= Q6ZGV8_ORYSJ (5e−81); GO_MF:GO:0005488, binding# (5e−81); 8 73.583489976 83493185 GO_BP:GO:0003743, protein-synthesizing GTPaseactivity, initiation# (5e−81); GO_CC:GO:0005634, nucleus# (1e−36) 633D8Ertd354e protein, putative n = 2 Tax = Oryza sativa RepID =Q53NA2_ORYSJ (8e−41); DUF2261: Uncharacterized conserved protein (DU(4e−09); 8 73.5 83733935 83740029 GO_MF:GO:0005488, binding# (3e−71);GO_BP:GO:0005975, carbohydrate metabolic process# (1e−28) 634 Cinnamicacid 4-hydroxylase n = 4 Tax = Andropogoneae RepID = Q94IP1_SORBI (0.0);p450: Cytochrome P450 (5.7e−134); GO_MF:GO:0046872, metal 8 73.583792776 83796118 ion binding# (0.0); GO_BP:GO:0055114, oxidationreduction# (0.0); GO_CC:GO:0016020, membrane# (0.0) 635 GRP: Glycinerich protein family (0.038) 8 73.5 83798547 83800359 636 Ankyrin likeprotein n = 1 Tax = Zea mays RepID = B6U4R6_MAIZE (0.0); DUF248:Putative methyltransferase (3.6e−284); Methyltransf_11:Methyltransferase 8 73.5 83916814 83921399 domain (3.2e−07);Methyltransf_12: Methyltransferase domain (0.04); GO_MF:GO:0046872,metal ion binding# (1e−128); GO_CC:GO:0005794, IDA#Golgi apparatus#(0.0) 637 F6D8.18 protein n = 11 Tax = rosids RepID = Q9SSR2_ARATH(7e−86); Peptidase_S24: Peptidase family S24 (1.5e−08);GO_MF:GO:0008233, peptidase 8 73.5 84066155 84070657 activity# (1e−92);GO_BP:GO:0006508, proteolysis# (1e−92); GO_CC:GO:0016020, membrane#(1e−92) 638 Mom(Plant), putative n = 1 Tax = Ricinus communis RepID =B9STU6_RICCO (6e−17); GO_MF:GO:0046872, metal ion binding# (5e−21); 873.5 84130984 84132037 GO_BP:GO:0006333, chromatin assembly ordisassembly# (5e−21); GO_CC:GO:0005634, nucleus# (5e−21) 639 Elongationfactor 1-alpha n = 112 Tax = Embryophyta RepID = EF1A_ARATH (0.0);GTP_EFTU: Elongation factor Tu GTP binding domain (1.3e−111); 8 73.584356485 84359671 MMR_HSR1: GTPase of unknown function (0.002);GTP_EFTU_D2: Elongation factor Tu domain 2 (8e−25); GTP_EFTU_D3:Elongation factor Tu C-terminal domain (8.5e−60); GO_MF:GO:0005525, GTPbinding# (0.0); GO_BP:GO:0046686, IEP#response to cadmium ion# (0.0);GO_CC:GO:0016020, membrane# (0.0) 640 Putative uncharacterized proteinSb08g000470 n = 5 Tax = Andropogoneae RepID = C5YQ14_SORBI (5e−22) 873.5 84404874 84411025 641 Kelch motif family protein n = 1 Tax = Zeamays RepID = B6TNH4_MAIZE (7e−32); GO_MF:GO:0016874, ligase activity#(2e−13); 8 73.5 84412206 84412417 GO_CC:GO:0005634, nucleus# (7e−17) 642Putative polyprotein n = 1 Tax = Oryza sativa Japonica Group RepID =Q688L2_ORYSJ (1e−50); GO_MF:GO:0003677, DNA binding# (1e−50); 8 73.584501053 84501763 GO_BP:GO:0015074, DNA integration# (1e−50) 643Retrotransposon protein, putative, unclassified n = 1 Tax = Oryza sativaJaponica Group RepID = Q2QMU2_ORYSJ (4e−59); Retrotrans_gag: 8 73.584501783 84503163 Retrotransposon gag protein (0.014); GO_MF:GO:0003677,DNA binding# (2e−43); GO_BP:GO:0015074, DNA integration# (2e−43) 644Retrotransposon protein, putative, unclassified n = 1 Tax = Oryza sativaJaponica Group RepID = Q2QUJ7_ORYSJ (2e−21); GO_MF:GO:0003676, 8 73.584594422 84594682 nucleic acid binding# (2e−21); GO_BP:GO:0006278,RNA-dependent DNA replication# (2e−20) 645 Elongation factor 1-alpha n =112 Tax = Embryophyta RepID = EF1A_ARATH (0.0); GTP_EFTU: Elongationfactor Tu GTP binding domain (1.3e−111); 8 73.5 84595221 84610050MMR_HSR1: GTPase of unknown function (0.002); GTP_EFTU_D2: Elongationfactor Tu domain 2 (1.8e−24); GTP_EFTU_D3: Elongation factor TuC-terminal domain (8.5e−60); GO_MF:GO:0005525, GTP binding# (0.0);GO_BP:GO:0046686, IEP#response to cadmium ion# (0.0); GO_CC:GO:0016020,membrane# (0.0) 646 GDP-mannose transporter, putative n = 1 Tax =Ricinus communis RepID = B9SA23_RICCO (2e−31); GO_MF:GO:0031072, heatshock protein binding# (8e−39); 8 73.5 84635631 84637805GO_BP:GO:0015784, IGI#GDP-mannose transport# (2e−29); GO_CC:GO:0016021,integral to membrane# (1e−42) 647 Putative gypsy-type retrotransposon n= 1 Tax = Zea mays RepID = Q7XBE0_MAIZE (2e−33) 8 73.5 84730931 84731620648 RNA binding protein n = 1 Tax = Zea mays RepID = B6UFA0_MAIZE(1e−175); RRM_1: RNA recognition motif. (a.k.a. RRM, RB (6.8e−10);RRM_1: 8 73.5 84784663 84789837 RNA recognition motif. (a.k.a. RRM, RB(1.1e−18); RRM_1: RNA recognition motif. (a.k.a. RRM, RB (1.3e−20);GO_MF:GO:0003723, RNA binding# (0.0); GO_BP:GO:0006397, mRNA processing#(2e−63); GO_CC:GO:0030529, ribonucleoprotein complex# (0.0) 649 FOG: TPRrepeat (ISS) n = 1 Tax = Ostreococcus tauri RepID = Q018P5_OSTTA(7e−09); TPR_1: Tetratricopeptide repeat (0.034); 8 73.5 8494363584947592 GO_MF:GO:0031072, heat shock protein binding# (1e−178) 650 DNAJheat shock N-terminal domain-containing protein n = 1 Tax =Polysphondylium pallidum PN500 RepID = D3AYV5_POLPA (8e−28); TPR_1: 873.5 84946695 84987693 Tetratricopeptide repeat (0.64); TPR_2:Tetratricopeptide repeat (0.23); TPR_1: Tetratricopeptide repeat(0.00055); TPR_2: Tetratricopeptide repeat (0.18); TPR_1:Tetratricopeptide repeat (0.26); TPR_2: Tetratricopeptide repeat (0.74);DnaJ: DnaJ domain (1.5e−26); GO_MF:GO:0031072, heat shock proteinbinding# (1e−149); GO_BP:GO:0006457, protein folding# (5e−26);GO_CC:GO:0045335, IDA#phagocytic vesicle# (3e−24) 651 Putativeuncharacterized protein Sb09g007350 n = 1 Tax = Sorghum bicolor RepID =C5YV43_SORBI (1e−40) 8 73.5 84958603 84960030 652 Lichenase-2 n = 2 Tax= Zea mays RepID = B6T391_MAIZE (1e−159); Glyco_hydro_17: Glycosylhydrolases family 17 (1.4e−175); 8 73.5 85097445 85103507GO_MF:GO:0043169, cation binding# (1e−159); GO_BP:GO:0008152, metabolicprocess# (1e−159); GO_CC:GO:0005615, extracellular space# (1e−79) 653Putative uncharacterized protein n = 1 Tax = Zea mays RepID =C0PE12_MAIZE (0.0); GO_MF:GO:0005488, binding# (0.0); 8 73.5 8512097585124053 GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0016020,membrane# (2e−72) 654 Calcineurin B-like protein 2 n = 18 Tax =Magnoliophyta RepID = CNBL2_ORYSJ (1e−37); GO_MF:GO:0005509, calcium ionstorage activity# (1e−37); 8 73.5 85274355 85274697 GO_BP:GO:0019722,IMP#calcium-mediated signaling# (7e−33); GO_CC:GO:0032578, aleuronegrain membrane# (1e−37) 655 Ribonuclease 3-like protein 2 n = 1 Tax =Oryza sativa Japonica Group RepID = RTL2_ORYSJ (1e−133); Ribonuclease_3:RNase3 domain (2.2e−29); dsrm: 8 73.5 85306646 85317194 Double-strandedRNA binding motif (5.7e−09); dsrm: Double-stranded RNA binding motif(7.7e−12); GO_MF:GO:0046872, metal ion binding# (1e−133);GO_BP:GO:0006396, RNA processing# (1e−133); GO_CC:GO:0005622,intracellular# (1e−133) 656 Zgc:92172 n = 2 Tax = Danio rerio RepID =Q5U399_DANRE (4e−33); GO_MF:GO:0016818, hydrolase activity, acting onacid anhydrides, in 8 73.5 85398753 85399187 phosphorus-containinganhydrides# (4e−45); GO_BP:GO:0006139, nucleobase, nucleoside,nucleotide and nucleic acid metabolic process# (4e−45);GO_CC:GO:0005634, nucleus# (4e−45) 657 Zgc:92172 n = 2 Tax = Danio rerioRepID = Q5U399_DANRE (4e−45); GO_MF:GO:0016818, hydrolase activity,acting on acid anhydrides, 8 73.5 85414178 85415861 inphosphorus-containing anhydrides# (3e−94); GO_BP:GO:0006139, nucleobase,nucleoside, nucleotide and nucleic acid metabolic process# (3e−94);GO_CC:GO:0005634, nucleus# (3e−94) 658 Putative uncharacterized proteinSb10g008850 n = 3 Tax = Andropogoneae RepID = C5Z7L1_SORBI (2e−10);GO_MF:GO:0047334, 8 73.5 85423276 85423801diphosphate-fructose-6-phosphate 1-phosphotransferase activity# (2e−10);GO_BP:GO:0047334, diphosphate-fructose-6-phosphate 1-phosphotransferaseactivity# (2e−10); GO_CC:GO:0005945, 6-phosphofructokinase complex#(2e−10) 659 CCT motif family protein n = 2 Tax = Zea mays RepID =B6U5M8_MAIZE (8e−11); GO_MF:GO:0051082, unfolded protein binding#(4e−09); 8 73.5 85657079 85668765 GO_BP:GO:0006950, response to stress#(4e−09) 660 Cation proton exchanger (Fragment) n = 2 Tax = Populustrichocarpa RepID = B9HXD0_POPTR (0.0); Na_H_Exchanger: Sodium/hydrogenexchanger 8 73.5 85863835 85866511 family (5.8e−83); GO_MF:GO:0015299,solute:hydrogen antiporter activity# (0.0); GO_BP:GO:0055085,transmembrane transport# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 661 Putative uncharacterized protein n = 2 Tax = Zea mays RepID =B4FIH8_MAIZE (3e−62) 8 73.5 86063082 86064559 662 Putativeuncharacterized protein Sb01g038930 n = 2 Tax = Andropogoneae RepID =C5WP90_SORBI (3e−23) 8 73.5 86316923 86317888 663 Chloroplastpentatricopeptide repeat protein 10 n = 2 Tax = Andropogoneae RepID =B8Y6I0_MAIZE (3e−17); PPR: PPR repeat (8.9e−06) 8 73.5 86570622 86571155664 Pectinesterase n = 1 Tax = Sorghum bicolor RepID = C5YWT6_SORBI(0.0); Got1: Got1-like family (0.057); PMEI: Plant invertase/pectinmethylesterase 8 73.5 86579079 86586531 inhibitor (8.5e−22);Pectinesterase: Pectinesterase (2.1e−191); GO_MF:GO:0045330, aspartylesterase activity# (0.0); GO_BP:GO:0042545, cell wall modification#(0.0); GO_CC:GO:0005618, IDA#cell wall# (0.0) 665 Protein bindingprotein n = 2 Tax = Zea mays RepID = B4FHI4_MAIZE (1e−35);GO_MF:GO:0008270, zinc ion binding# (1e−35) 8 73.5 86661357 86661949 666Gag-pol n = 1 Tax = Zea mays RepID = Q8W1D1_MAIZE (2e−83); rve:Integrase core domain (3.6e−15); GO_MF:GO:0008270, zinc ion binding#(2e−83); 8 73.5 86670796 86671275 GO_BP:GO:0015074, DNA integration#(2e−83); GO_CC:GO:0005634, nucleus# (2e−83) 667 Pentatricopeptiderepeat-containing protein, putative n = 1 Tax = Ricinus communis RepID =B9RZB6_RICCO (1e−104); PfkB: pfkB family carbohydrate 8 73.5 8686170586871530 kinase (8.2e−12); GO_MF:GO:0004747, ribokinase activity#(1e−166); GO_BP:GO:0006014, D-ribose metabolic process# (1e−166);GO_CC:GO:0005622, intracellular# (6e−25) 668 Catalytic/proteinphosphatase type 2C n = 2 Tax = Zea mays RepID = B6TWB0_MAIZE (4e−25);GO_MF:GO:0003824, catalytic activity# (4e−25); 8 73.5 GO_BP:GO:0004721,phosphoprotein phosphatase activity# (2e−22); GO_CC:GO:0005886, plasmamembrane# (7e−14) 669 DNA primase n = 1 Tax = Zea mays RepID =B6T4S3_MAIZE (0.0); DNA_primase_S: DNA primase small subunit (1.6e−52);GO_MF:GO:0016740, 8 73.5 transferase activity# (0.0); GO_BP:GO:0006269,DNA replication, synthesis of RNA primer# (0.0); GO_CC:GO:0005658, alphaDNA polymerase:primase complex# (0.0) 670 WRKY67-superfamily of TFshaving WRKY and zinc finger domains n = 2 Tax = Zea mays RepID =B6T4Y9_MAIZE (6e−83); FAR1: FAR1 family (0.018); 8 73.6 8896391288965293 WRKY: WRKY DNA-binding domain (1.7e−36); GO_MF:GO:0043565,sequence-specific DNA binding# (6e−83); GO_BP:GO:0045449, regulation oftranscription# (6e−83); GO_CC:GO:0005634, nucleus# (6e−83) 671Transposon protein, putative, CACTA, En/Spm sub-class n = 1 Tax = Oryzasativa Japonica Group RepID = Q53MW6_ORYSJ (2e−16); GO_MF:GO:0004803, 873.7 78314202 78314952 transposase activity# (1e−22); GO_BP:GO:0006313,transposition, DNA-mediated# (1e−22) 672 Transposon protein, putative,CACTA, En/Spm sub-class n = 1 Tax = Oryza sativa Japonica Group RepID =Q53MW6_ORYSJ (3e−30); GO_MF:GO:0004803, 8 73.7 78314968 78316948transposase activity# (8e−24); GO_BP:GO:0006313, transposition,DNA-mediated# (8e−24) 673 F-box domain containing protein n = 2 Tax =Oryza sativa RepID = Q7XH06_ORYSJ (7e−43); F-box: F-box domain(1.8e−05); GO_MF:GO:0008270, zinc ion 8 73.7 86905453 86908820 binding#(2e−50); GO_CC:GO:0005622, intracellular# (2e−50) 674 H0215A08.3 proteinn = 1 Tax = Oryza sativa RepID = Q01N39_ORYSA (1e−115); RVT_1: Reversetranscriptase (RNA-dependent DN (7e−37); 8 73.8 78350122 78351597GO_MF:GO:0003964, RNA-directed DNA polymerase, group II intron encoded#(1e−115); GO_BP:GO:0006278, RNA-dependent DNA replication# (1e−115);GO_CC:GO:0005634, nucleus# (1e−115) 675 Non-cyanogenic beta-glucosidasen = 2 Tax = Zea mays RepID = B6SKG0_MAIZE (9e−27); GO_MF:GO:0043169,cation binding# (9e−27); 8 73.8 86911578 86914687 GO_BP:GO:0005975,carbohydrate metabolic process# (9e−27); GO_CC:GO:0005773, IDA#vacuole#(2e−13) 676 Cysteine-type peptidase n = 6 Tax = Poaceae RepID =B6ST73_MAIZE (1e−16) 8 73.8 86920313 86927376 677 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B4FS85_MAIZE(3e−11) 8 73.8 86941607 86942715 678 Putative polyprotein n = 1 Tax =Oryza sativa Japonica Group RepID = Q6ATH7_ORYSJ (1e−51);GO_MF:GO:0004523, ribonuclease H activity# (1e−51); 8 73.8 8698194386982509 GO_BP:GO:0045449, regulation of transcription# (1e−51) 679Monoglyceride lipase n = 2 Tax = Andropogoneae RepID = B4FRE0_MAIZE(1e−146); Abhydrolase_1: alpha/beta hydrolase fold (4e−05); 8 73.887027773 87035014 GO_MF:GO:0047372, acylglycerol lipase activity#(1e−131); GO_CC:GO:0005886, plasma membrane# (1e−132) 680UDP-D-glucuronate decarboxylase (Fragment) n = 1 Tax = Hordeum vulgareRepID = Q6B6L9_HORVU (1e−180); Rm1D_sub_bind: Rm1D substrate binding 873.8 87033480 87038152 domain (7.8e−05); Epimerase: NAD dependentepimerase/dehydratase family (7.9e−56); Polysacc_synt_2: Polysaccharidebiosynthesis protein (0.00074); 3Beta_HSD: 3-beta hydroxysteroiddehydrogenase/isomerase family (1.7e−05); NAD_binding_4: Male sterilityprotein (3.5e−07); GO_MF:GO:0050662, coenzyme binding# (0.0);GO_BP:GO:0044237, cellular metabolic process# (0.0); GO_CC:GO:0016020,membrane# (1e−153) 681 OSJNBa0093O08.9 protein n = 3 Tax = Oryza sativaRepID = Q7XTN8_ORYSJ (1e−30); GO_MF:GO:0017111,nucleoside-triphosphatase activity# (1e−30) 8 73.8 87151830 87152641 682Nucleic acid binding protein n = 3 Tax = Andropogoneae RepID =B6U4M2_MAIZE (1e−35); GO_MF:GO:0003676, nucleic acid binding# (1e−35); 873.8 87152728 87154011 GO_CC:GO:0005622, intracellular# (1e−35) 683Catalytic/oxidoreductase, acting on NADH or NADPH n = 3 Tax = Zea maysRepID = B6T0D7_MAIZE (6e−20); Complex1_LYR: Complex 1 protein 8 73.887238623 87243402 (LYR family) (0.00071) 684 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = C0HEY7_MAIZE (4e−89) 8 73.887334958 87340970 685 Inner membrane lipoprotein n = 6 Tax = EscherichiaRepID = B7MNA9_ECO45 (1e−103); GO_BP:GO:0009405, ice nucleationactivity# (2e−56); 8 73.8 87361662 87362393 GO_CC:GO:0016020, membrane#(1e−120) 686 Inner membrane lipoprotein n = 6 Tax = Escherichia RepID =B7LGB2_ECO55 (1e−125); DiS_P_DiS: Bacterial Peptidase A24 N-terminaldomai (2.1e−48); 8 73.8 87362416 87364280 GO_MF:GO:0004190,penicillopepsin activity# (9e−89); GO_BP:GO:0009405, ice nucleationactivity# (2e−55); GO_CC:GO:0016020, membrane# (1e−126) 687 Generalsecretion pathway protein C n = 18 Tax = Escherichia RepID =D3QRC8_ECOLX (1e−128); GspL: General secretion pathway protein L (GspL)(1e−47); 8 73.8 87364807 87367103 FliL: Flagellar basal body-associatedprotein FliL (0.022); GspM: General secretion pathway, M protein(8.2e−71); GO_MF:GO:0008565, protein transporter activity# (1e−135);GO_BP:GO:0015628, protein secretion by the type II secretion system#(1e−135); GO_CC:GO:0042597, periplasmic space# (1e−135) 688 Nucleosidepermease (Fragment) n = 1 Tax = Escherichia coli RepID = B8ZYJ8_ECOLX(1e−106); Nuc_H_symport: Nucleoside H+ symporter (4e−34); 8 73.887368603 87369175 GO_MF:GO:0005337, nucleoside transmembrane transporteractivity# (1e−107); GO_BP:GO:0015858, IMP#nucleoside transport#(1e−107); GO_CC:GO:0016021, integral to membrane# (1e−107) 689 Ornithinedecarboxylase isozyme n = 5 Tax = Klebsiella RepID = C4WZ59_KLEPN (0.0);OKR_DC_1_N: Orn/Lys/Arg decarboxylase, N-terminal domain 8 73.8 8736932087371362 (6.5e−33); OKR_DC_1: Orn/Lys/Arg decarboxylase, major domain(1.4e−257); OKR_DC_1_C: Orn/Lys/Arg decarboxylase, C-terminal domain(0.0023); GO_MF:GO:0030170, pyridoxal phosphate binding# (0.0);GO_BP:GO:0006520, cellular amino acid metabolic process# (0.0);GO_CC:GO:0005737, cytoplasm# (0.0) 690 YqgA n = 31 Tax = Salmonellaenterica RepID = B4T5M5_SALNS (5e−25); DUF554: Protein of unknownfunction (DUF554) (6.2e−05); GO_MF:GO:0005216, 8 73.8 87372130 87372343ion channel activity# (2e−12); GO_BP:GO:0005216, ion channel activity#(2e−12); GO_CC:GO:0016021, integral to membrane# (9e−32) 691Chromodomain helicase DNA binding protein, putative n = 1 Tax = Ricinuscommunis RepID = B9SYQ4_RICCO (2e−19); Chromo: ‘chromo’ (CHRromatin 873.8 87478622 87479964 Organisation MOd (1.8e−12); GO_MF:GO:0005524, ATPbinding# (3e−27); GO_BP:GO:0006333, chromatin assembly or disassembly#(3e−27); GO_CC:GO:0005634, nucleus# (3e−27) 692 Putative uncharacterizedprotein Sb07g027880 n = 1 Tax = Sorghum bicolor RepID = C5YIT8_SORBI(3e−26) 8 73.8 87482396 87483166 693 Chaperone protein dnaJ, putative n= 1 Tax = Ricinus communis RepID = B9RT65_RICCO (1e−74); DnaJ: DnaJdomain (2e−30); DUF1977: Domain of unknown 8 73.8 87494732 87498179function (DUF1977) (4.7e−27); GO_MF:GO:0051082, unfolded proteinbinding# (1e−130); GO_BP:GO:0006457, protein folding# (1e−130);GO_CC:GO:0016021, integral to membrane# (1e−55) 694 MLO-like protein 4 n= 3 Tax = Andropogoneae RepID = B6TXU3_MAIZE (1e−145); Mlo: Mlo family(3.8e−45); Clathrin: Region in Clathrin and VPS (1.8e−15); 8 73.887517374 87524631 GO_MF:GO:0005515, protein binding# (7e−97);GO_BP:GO:0008219, TAS#cell death# (1e−145); GO_CC:GO:0016021, integralto membrane# (1e−145) 695 Phytochrome A-associated F-box protein,putative n = 1 Tax = Ricinus communis RepID = B9SAQ8_RICCO (8e−71);GO_MF:GO:0003676, nucleic acid 8 73.8 87544180 87545930 binding#(2e−69); GO_BP:GO:0048573, IMP#photoperiodism, flowering# (2e−60);GO_CC:GO:0005634, nucleus# (2e−60) 696 Hemolysin n = 1 Tax = Zea maysRepID = B6SVJ7_MAIZE (1e−107); DUF21: Domain of unknown function DUF21(2.4e−42); CBS: CBS domain (5.6e−07); 8 73.8 87676566 87726869GO_MF:GO:0003677, DNA binding# (1e−148); GO_BP:GO:0015074, DNAintegration# (1e−148); GO_CC:GO:0005739, mitochondrion# (1e−153) 697 HATfamily dimerisation domain containing protein n = 1 Tax = Oryza sativaJaponica Group RepID = Q2QP98_ORYSJ (5e−12); GO_MF:GO:0046983, 8 73.887692227 87692768 protein dimerization activity# (8e−13);GO_BP:GO:0015074, DNA integration# (1e−11); GO_CC:GO:0005622,intracellular# (7e−12) 698 Putative uncharacterized proteinSb0010s003460 n = 1 Tax = Sorghum bicolor RepID = C6JRI0_SORBI (1e−26);zf-CCHC: Zinc knuckle (0.0081); zf-CCHC: 8 73.8 87707935 87722864 Zincknuckle (0.00027); GO_MF:GO:0043565, sequence-specific DNA binding#(5e−11); GO_BP:GO:0045449, regulation of transcription# (5e−11);GO_CC:GO:0005634, nucleus# (5e−11) 699 Retrotransposon protein,putative, unclassified n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QXX8_ORYSJ (2e−10); GO_MF:GO:0003677, 8 73.8 87722867 87723474 DNAbinding# (2e−10); GO_BP:GO:0015074, DNA integration# (2e−10) 700Putative polyprotein n = 1 Tax = Oryza sativa Japonica Group RepID =Q688L2_ORYSJ (3e−30); rve: Integrase core domain (0.004);GO_MF:GO:0003677, 8 73.8 87723686 87724030 DNA binding# (2e−30);GO_BP:GO:0015074, DNA integration# (2e−30) 701 Putative uncharacterizedprotein Sb09g019530 n = 1 Tax = Sorghum bicolor RepID = C5YXL1_SORBI(2e−17) 8 73.8 87727511 87728085 702 Retrotransposon protein n = 1 Tax =Zea mays RepID = B6U894_MAIZE (1e−100); DUF889: Eukaryotic protein ofunknown function (DUF889) (8.1e−22); 8 73.8 87772742 87776398Rep_fac-A_C: Replication factor-A C terminal domain (0.0031);GO_MF:GO:0004386, helicase activity# (1e−57) 703 OSJNBa0095H06.12protein n = 1 Tax = Oryza sativa Japonica Group RepID = Q7XS07_ORYSJ(1e−21); GO_MF:GO:0004386, helicase activity# (1e−19) 8 73.8 8777652487776994 704 Putative retrotransposon protein n = 1 Tax = Phyllostachysedulis RepID = D3IVP0_9POAL (0.0); GO_MF:GO:0004386, helicase activity#(1e−148) 8 73.8 87777078 87782262 705 Kinesin-4 n = 1 Tax = Zea maysRepID = B6U113_MAIZE (0.0); Kinesin: Kinesin motor domain (3.3e−157);GO_MF:GO:0005524, ATP binding# (0.0); 8 73.8 87802577 87807005GO_BP:GO:0007018, microtubule-based movement# (0.0); GO_CC:GO:0005874,microtubule# (0.0) 706 Putative uncharacterized protein Sb03g045470 n =1 Tax = Sorghum bicolor RepID = C5XHR9_SORBI (3e−22); GO_MF:GO:0016787,hydrolase activity# (1e−18); 8 73.85 78352728 78353780 GO_CC:GO:0016020,membrane# (3e−13) 707 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = B6T065_MAIZE (2e−16) 8 73.85 87763115 87797399 708 FtsH4 n= 3 Tax = Triticeae RepID = C6ERB5_AEGTA (0.0); FtsH_ext: FtsHExtracellular (0.015); AAA_2: ATPase family associated with various(0.021); 8 73.9 78358305 78365508 AAA: ATPase family associated withvarious cellular activities (AAA) (6.7e−94); AAA_5: ATPase familyassociated with various (4.5e−05); Peptidase_M41: Peptidase family M41(2e−111); GO_MF:GO:0046872, metal ion binding# (0.0); GO_BP:GO:0030163,protein catabolic process# (0.0); GO_CC:GO:0016020, membrane# (0.0) 709Retrotransposon protein, putative, unclassified n = 1 Tax = Oryza sativaJaponica Group RepID = Q10QE5_ORYSJ (4e−13); GO_MF:GO:0003964,RNA-directed DNA 8 73.9 78363973 78364305 polymerase, group II intronencoded# (4e−13); GO_BP:GO:0006278, RNA-dependent DNA replication#(4e−13) 710 Serine−threonine protein kinase, plant-type, putative n = 1Tax = Ricinus communis RepID = B9RTG0_RICCO (4e−22); GO_MF:GO:0005524,ATP binding# (7e−62); 8 73.9 78365952 78366617 GO_BP:GO:0016998, cellwall macromolecule catabolic process# (7e−63) 711 PR-1-like protein(Fragment) n = 1 Tax = Zea mays RepID = D0EJL7_MAIZE (1e−37); SCP:SCP-like extracellular protein (6.5e−29); GO_BP:GO:0009607, response tobiotic 8 73.9 79003686 79004902 stimulus# (2e−21); GO_CC:GO:0005576,extracellular region# (3e−49) 712 HYP1 n = 2 Tax = Andropogoneae RepID =B6SS81_MAIZE (0.0); DUF221: Domain of unknown function DUF221(3.6e−138); GO_CC:GO:0016020, membrane# (0.0) 8 73.9 79005811 79011465713 OSJNBa0033G05.13 protein n = 1 Tax = Oryza sativa Japonica GroupRepID = Q7XTM9_ORYSJ (5e−44); rve: Integrase core domain (7.3e−08);GO_MF:GO:0046872, metal ion 8 73.9 87867597 87868246 binding# (4e−44);GO_BP:GO:0015074, DNA integration# (4e−44); GO_CC:GO:0005622,intracellular# (2e−43) 714 Oxidoreductase, 2OG—Fe oxygenase familyprotein n = 1 Tax = Zea mays RepID = B6ST02_MAIZE (2e−58);GO_MF:GO:0016491, oxidoreductase activity# (3e−71); 8 73.9 8803984688047578 GO_BP:GO:0055114, oxidation reduction# (2e−58);GO_CC:GO:0005622, intracellular# (2e−17) 715 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = B6U3C1_MAIZE (2e−42) 8 73.988139482 88142331 716 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = B6U3C1_MAIZE (1e−26) 8 73.9 88149082 88149658 717H0512B01.8 protein n = 1 Tax = Oryza sativa RepID = Q25AF6_ORYSA(2e−84); zf-CCHC: Zinc knuckle (0.0019); GO_MF:GO:0046872, metal ionbinding# (2e−84); 8 73.9 88172438 88173775 GO_BP:GO:0015074, DNAintegration# (2e−84) 718 Retrotransposon protein, putative, Ty3-gypsysubclass n = 1 Tax = Oryza sativa Japonica Group RepID = Q109Z1_ORYSJ(1e−123); zf-CCHC: Zinc knuckle (8.1e−06); 8 73.9 88184506 88186242GO_MF:GO:0008270, zinc ion binding# (1e−123); GO_BP:GO:0015074, DNAintegration# (1e−121); GO_CC:GO:0005634, nucleus# (1e−117) 719 Putativeuncharacterized protein Sb09g019730 n = 1 Tax = Sorghum bicolor RepID =C5YXN0_SORBI (2e−55); GO_MF:GO:0005509, calcium ion storage activity#(2e−50); 8 73.9 88275115 88276667 GO_BP:GO:0015979, photosynthesis#(2e−50); GO_CC:GO:0019898, ISS#extrinsic to membrane# (2e−50) 720 ATPsynthase subunit alpha, mitochondrial n = 51 Tax = Eukaryota RepID =ATPAM_ARATH (2e−54); ATP-synt_ab: ATP synthase alpha/beta family,nucleotide-binding domain 8 73.9 88341941 88342255 (3e−09);GO_MF:GO:0046961, proton-transporting ATPase activity, rotationalmechanism# (2e−54); GO_BP:GO:0046961, proton-transporting ATPaseactivity, rotational mechanism# (2e−54); GO_CC:GO:0045261,proton-transporting ATP synthase complex, catalytic core F(1)# (2e−54)721 PnFL-2 n = 3 Tax = Andropogoneae RepID = B6T7Q6_MAIZE (2e−29) 8 73.988398162 88398597 722 Beta-propeller domains of methanol dehydrogenasetype n = 2 Tax = Andropogoneae RepID = B6U4G5_MAIZE (1e−129); DUF477:Protein of unknown function (DUF477) (1.2e−26); 8 73.9 88438855 88440306GO_CC:GO:0016021, integral to membrane# (6e−95) 723 Basic endochitinaseA n = 1 Tax = Zea mays RepID = B6TR38_MAIZE (1e−155); Chitin_bind_1:Chitin recognition protein (2e−16); Glyco_hydro_19: Chitinase class I(2.5e−172); 8 73.9 88812632 88814171 GO_MF:GO:0016798, hydrolaseactivity, acting on glycosyl bonds# (1e−155); GO_BP:GO:0016998, cellwall macromolecule catabolic process# (1e−155); GO_CC:GO:0005576,extracellular region# (1e−111) 724 Calmodulin binding protein n = 1 Tax= Zea mays RepID = B6SPC3_MAIZE (1e−139); GO_BP:GO:0010200, IEP#responseto chitin# (1e−115) 8 74 78407583 78408959 725 Histone H2A n = 7 Tax =Spermatophyta RepID = A5AKG7_VITVI (3e−43); Histone: Core histoneH2A/H2B/H3/H4 (3.2e−15); GO_MF:GO:0003677, DNA binding# (3e−43); 8 7478891417 78892066 GO_BP:GO:0006334, nucleosome assembly# (3e−43);GO_CC:GO:0005694, chromosome# (3e−43) 726 Nodulin-like protein n = 2 Tax= Oryza sativa RepID = Q6ZG27_ORYSJ (3e−33); Nodulin-like: Nodulin-like(4.5e−83); GO_MF:GO:0031072, heat shock protein binding# (2e−26); 8 7478939868 78940608 GO_BP:GO:0055085, transmembrane transport# (7e−42);GO_CC:GO:0005634, nucleus# (5e−30) 727 Cobalt ion transporter, putativen = 1 Tax = Ricinus communis RepID = B9RST4_RICCO (1e−86); CbiQ: Cobalttransport protein (3e−09); GO_MF:GO:0015087, cobalt ion 8 74 8854626788567647 transmembrane transporter activity# (1e−118); GO_BP:GO:0015087,cobalt ion transmembrane transporter activity# (1e−118);GO_CC:GO:0016021, integral to membrane# (2e−77) 728 Heat shock protein90 n = 6 Tax = Oryza sativa RepID = Q5Z9N8_ORYSJ (2e−70);GO_MF:GO:0051082, unfolded protein binding# (2e−70); GO_BP:GO:0006950,response to 8 74 88673927 88675485 stress# (2e−70); GO_CC:GO:0005737,cytoplasm# (2e−70) 729 Plasma membrane associated protein n = 2 Tax =Andropogoneae RepID = B6UGF3_MAIZE (1e−83); AWPM-19: AWPM-19-like family(4e−86); GO_CC:GO:0016021, integral 8 74 88678931 88680028 to membrane#(1e−12) 730 Serine/threonine-protein kinase, putative n = 1 Tax =Ricinus communis RepID = B9RQT0_RICCO (0.0); Pkinase: Protein kinasedomain (8.7e−80); Pkinase_Tyr: Protein tyrosine 8 74 89080382 89093271kinase (1.5e−12); Pkinase_C: Protein kinase C terminal domain (7.4e−09);GO_MF:GO:0016740, transferase activity# (0.0); GO_BP:GO:0016301, kinaseactivity# (0.0); GO_CC:GO:0005886, plasma membrane# (1e−179) 731Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6TUN3_MAIZE (5e−29) 8 74 89154975 89158300 732 BZIP transcriptionfactor bZIP109 n = 3 Tax = Glycine max RepID = Q0GPG4_SOYBN (6e−33);DUF1664: Protein of unknown function (DUF1664) (2.4e−11) 8 74 8925725689260917 733 Late embryogenesis abundant protein n = 3 Tax = Zea maysRepID = B6TTU1_MAIZE (1e−47) 8 74 89528694 89529652 734 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C4JA86_MAIZE(9e−23) 8 74 89583965 89643253 735 OSJNBa0022H21.18 protein n = 2 Tax =Oryza sativa Japonica Group RepID = Q7XKT9_ORYSJ (3e−36); Thiolase_N:Thiolase, N-terminal domain (0.042); 8 74 89643922 89646684GO_MF:GO:0048038, quinone binding# (3e−40); GO_BP:GO:0055114, oxidationreduction# (3e−40); GO_CC:GO:0005777, IDA#peroxisome# (2e−24) 736Putative polyprotein n = 1 Tax = Oryza sativa Japonica Group RepID =Q6L559_ORYSJ (4e−79); rve: Integrase core domain (2.2e−07);GO_MF:GO:0008270, zinc ion binding# 8 74 89691497 89692201 (4e−79);GO_BP:GO:0015074, DNA integration# (4e−79); GO_CC:GO:0005840, ribosome#(4e−78) 737 Gag and Pol n = 1 Tax = Zea mays RepID = Q8LSK0_MAIZE(2e−31); GO_MF:GO:0008270, zinc ion binding# (2e−31); GO_BP:GO:0015074,DNA integration# (2e−31); 8 74 89692947 89693332 GO_CC:GO:0005840,ribosome# (2e−28) 738 Putative uncharacterized protein n = 3 Tax = Zeamays RepID = B6SLL9_MAIZE (6e−31) 8 74 89825230 89825970 739Retrotransposon protein, putative, unclassified n = 2 Tax = Oryza sativaRepID = Q10KN1_ORYSJ (1e−15); GO_MF:GO:0003964, RNA-directed DNApolymerase, group II 8 74 89924962 89925156 intron encoded# (1e−15);GO_BP:GO:0006278, RNA-dependent DNA replication# (1e−15);GO_CC:GO:0005634, nucleus# (3e−14) 740 Putative polyprotein n = 1 Tax =Zea mays RepID = Q8SA93_MAIZE (1e−34); GO_MF:GO:0004190, penicillopepsinactivity# (1e−34); GO_BP:GO:0006508, proteolysis# (1e−34); 8 74 8992525189925643 GO_CC:GO:0005634, nucleus# (1e−34) 741 Heat shock 70 kDaprotein 4 n = 1 Tax = Zea mays RepID = B6SV64_MAIZE (0.0); HSP70: Hsp70protein (1.9e−120); GO_MF:GO:0005524, ATP binding# (0.0); 8 74 8996427389967960 GO_BP:GO:0006950, response to stress# (0.0); GO_CC:GO:0005737,cytoplasm# (1e−127) 742 Casein kinase I-like n = 4 Tax = Poaceae RepID =Q8LR51_ORYSJ (0.0); Pkinase: Protein kinase domain (1.1e−42);Pkinase_Tyr: Protein tyrosine kinase (1.5e−05); 8 74.05 7840921078414387 GO_MF:GO:0005524, ATP binding# (0.0); GO_BP:GO:0006468, proteinamino acid phosphorylation# (0.0); GO_CC:GO:0005886, plasma membrane#(0.0) 743 NF protein (Fragment) n = 1 Tax = Oryza sativa Japonica GroupRepID = Q70KT0_ORYSJ (2e−30); GO_MF:GO:0016301, kinase activity#(7e−14); GO_BP:GO:0016301, kinase 8 74.1 78827462 78829082 activity#(7e−14); GO_CC:GO:0031588, AMP-activated protein kinase complex# (1e−09)744 Fb2 n = 3 Tax = Zea mays RepID = B6SGL8_MAIZE (2e−68); Di19: Droughtinduced 19 protein (Di19) (1.9e−08); GO_MF:GO:0008270, zinc ion binding#(7e−13); 8 74.1 78831850 78832799 GO_CC:GO:0005622, intracellular#(1e−12) 745 Os05g0594500 protein n = 3 Tax = Oryza sativa RepID =Q5TKG2_ORYSJ (0.0) 8 74.1 78853024 78857808 746 Heat shock 70 kDaprotein 4 n = 4 Tax = Andropogoneae RepID = B6U237_MAIZE (0.0); HSP70:Hsp70 protein (4.8e−162); GO_MF:GO:0005524, ATP binding# (0.0); 8 74.190052425 90060314 GO_BP:GO:0006950, response to stress# (0.0);GO_CC:GO:0005737, cytoplasm# (1e−166) 747 Nucleic acid binding f n = 1Tax = Zea mays RepID = B6T2M0_MAIZE (3e−79); GO_MF:GO:0046872, metal ionbinding# (3e−79) 8 74.1 90062911 90064339 748 Early fruit mRNA n = 3 Tax= Andropogoneae RepID = B6TAC1_MAIZE (2e−20) 8 74.1 90360744 90364222749 Dehydration-responsive protein-like n = 2 Tax = Oryza sativa RepID =Q653G1_ORYSJ (4e−69); DUF248: Putative methyltransferase (7.8e−21);tRNA_m1G_MT: tRNA 8 74.15 78437055 78440172(Guanine-1)-methyltransferase (0.00014); GO_MF:GO:0016740, transferaseactivity# (5e−55); GO_BP:GO:0016301, kinase activity# (2e−38);GO_CC:GO:0009505, IDA#expansin# (3e−37) 750 163k15.5 n = 1 Tax = Zeamays RepID = Q8S457_MAIZE (2e−30); MULE: MULE transposase domain(4.2e−17); GO_MF:GO:0008270, zinc ion binding# (2e−30); 8 74.15 9012641690136807 GO_BP:GO:0004867, chymotrypsin inhibitor activity# (6e−25) 751Putative uncharacterized protein n = 1 Tax = Zea mays RepID =C0PHV5_MAIZE (4e−12) 8 74.2 78462451 78462840 752 OSJNBa0055H05.12protein n = 2 Tax = Oryza sativa Japonica Group RepID = Q7XRD0_ORYSJ(1e−104); GO_MF:GO:0046872, metal ion binding# (1e−114) 8 74.2 7878497278787336 753 NEDD8-activating enzyme E1 catalytic subunit n = 3 Tax =Andropogoneae RepID = B6TJH0_MAIZE (5e−32); RRM_1: RNA recognitionmotif. (a.k.a. RRM, RB (0.037); 8 74.2 90141945 90143462GO_MF:GO:0016881, acid-amino acid ligase activity# (5e−32);GO_BP:GO:0045116, protein neddylation# (5e−32); GO_CC:GO:0005634,nucleus# (1e−18) 754 Receptor-like protein kinase n = 2 Tax = Zea maysRepID = B6TPE6_MAIZE (1e−179); LRRNT_2: Leucine rich repeat N-terminaldomain (0.053); LRR_1: Leucine Rich Repeat 8 74.2 90187637 90189879 (9);LRR_1: Leucine Rich Repeat (4.5); LRR_1: Leucine Rich Repeat (4); LRR_1:Leucine Rich Repeat (29); LRR_1: Leucine Rich Repeat (3e+02); LRR_1:Leucine Rich Repeat (41); LRR_1: Leucine Rich Repeat (1.6); LRR_1:Leucine Rich Repeat (21); GO_MF:GO:0016301, kinase activity# (1e−179);GO_BP:GO:0016301, kinase activity# (1e−179); GO_CC:GO:0009505,IDA#expansin# (1e−111) 755 Annexin p35 (Fragment) n = 1 Tax = Oryzasativa Indica Group RepID = C5IDU2_ORYSI (6e−10); GO_MF:GO:0005544,calcium-dependent phospholipid binding# (2e−13); 8 74.2 9019097390191783 GO_BP:GO:0046686, IEP#response to cadmium ion# (9e−09);GO_CC:GO:0048046, IDA#apoplast# (9e−09) 756 Transcription factor,putative n = 1 Tax = Ricinus communis RepID = B9SB74_RICCO (4e−32);GO_BP:GO:0051301, cell division# (7e−19); GO_CC:GO:0005634, nucleus#(7e−19) 8 74.2 90262045 90264571 757 Putative uncharacterized protein n= 1 Tax = Zea mays RepID = B6UGB2_MAIZE (7e−61) 8 74.2 90425639 90426136758 HAT family dimerisation domain containing protein n = 1 Tax = Oryzasativa Japonica Group RepID = Q2QRD1_ORYSJ (1e−74); GO_MF:GO:0046983,protein dimerization 8 74.2 90465136 90466806 activity# (1e−74) 759Putative GDA2 protein n = 2 Tax = Oryza sativa RepID = Q5JK83_ORYSJ(2e−92); Dev_Cell_Death: Development and cell death domain (3.6e−84);GO_MF:GO:0016779, 8 74.3 78649300 78651403 nucleotidyltransferaseactivity# (5e−34) 760 Calcium/proton exchanger CAX1-like protein n = 3Tax = Zea mays RepID = Q9LKW7_MAIZE (0.0); Na_Ca_ex: Sodium/calciumexchanger protein (5.1e−25); Na_Ca_ex: 8 74.3 78653714 78658162Sodium/calcium exchanger protein (4.7e−31); GO_MF:GO:0008324, cationtransmembrane transporter activity# (0.0); GO_BP:GO:0055085,transmembrane transport# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 761 Putative uncharacterized protein n = 1 Tax = Zea mays RepID =C0P6T6_MAIZE (6e−38) 8 74.3 90528889 90529392 762 3′-N-debenzoyltaxolN-benzoyltransferase-like n = 2 Tax = Oryza sativa RepID = Q9LGF6_ORYSJ(1e−165); Transferase: Transferase family (1.3e−50); GO_MF:GO:0016747, 874.3 90577557 90579826 transferase activity, transferring acyl groupsother than amino-acyl groups# (0.0) 763 Putative uncharacterized proteinSb09g005455 n = 1 Tax = Sorghum bicolor RepID = C5Z172_SORBI (3e−10);DVL: DVL family (1.1e−08) 8 74.4 90669153 90669662 764 Leucine zipperfactor-like n = 1 Tax = Oryza sativa Japonica Group RepID = Q5N7Y0_ORYSJ(4e−20); PWWP: PWWP domain (0.046); Sas10_Utp3: Sas10/Utp3 family(2.1e−14) 8 74.4 90687922 90692251 765 Ras-related protein RGP2 n = 7Tax = Poaceae RepID = RGP2_ORYSJ (1e−112); Arf: ADP-ribosylation factorfamily (0.00043); MMR_HSR1: GTPase of unknown function 8 74.4 9078925390791725 (0.0016); Miro: Miro-like protein (5.9e−24); Ras: Ras family(1.2e−96); GTP_EFTU: Elongation factor Tu GTP binding domain (0.069);GO_MF:GO:0005525, GTP binding# (1e−112); GO_BP:GO:0015031, proteintransport# (1e−112); GO_CC:GO:0016020, membrane# (1e−112) 766 Proteinkinase APK1B, chloroplast, putative n = 1 Tax = Ricinus communis RepID =B9SPN3_RICCO (9e−90); Pkinase: Protein kinase domain (1.5e−30);Pkinase_Tyr: Protein 8 74.4 90833086 90835652 tyrosine kinase (3.4e−17);APH: Phosphotransferase enzyme family (0.0022); GO_MF:GO:0005524, ATPbinding# (0.0); GO_BP:GO:0006468, protein amino acid phosphorylation#(0.0); GO_CC:GO:0005576, extracellular region# (1e−76) 767UDP-sulfoquinovose synthase n = 1 Tax = Solanum lycopersicum RepID =C0LIR3_SOLLC (0.0); Epimerase: NAD dependent epimerase/dehydratasefamily (1.6e−39); 8 74.5 90964519 90967651 GO_MF:GO:0050662, coenzymebinding# (0.0); GO_BP:GO:0044237, cellular metabolic process# (0.0);GO_CC:GO:0009536, plastid# (0.0) 768 Phosphatidylinositol transferprotein CSR1 n = 2 Tax = Zea mays RepID = B6TMQ2_MAIZE (5e−62);CRAL_TRIO_N: CRAL/TRIO, N-terminus (0.0023); 8 74.5 90968485 90972591GO_MF:GO:0005215, transporter activity# (1e−29); GO_BP:GO:0006810,transport# (1e−29); GO_CC:GO:0005622, intracellular# (1e−29) 769Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6T777_MAIZE (3e−86) 8 74.5 91022431 91023673 770 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6TWE0_MAIZE(8e−14) 8 74.7 91235607 91235966 771 Putative uncharacterized proteinSb09g019020 n = 1 Tax = Sorghum bicolor RepID = C5YXE2_SORBI (1e−106) 874.7 91312779 91313513 772 Putative uncharacterized protein Sb02g039805(Fragment) n = 1 Tax = Sorghum bicolor RepID = C5X2J3_SORBI (6e−13) 874.7 91361119 91361355 773 Mitochondria fission 1 protein n = 1 Tax =Zea mays RepID = B6T224_MAIZE (2e−63); GO_MF:GO:0005488, binding#(2e−63); GO_BP:GO:0016301, kinase activity# (1e−41); 8 74.7 9138517491386308 GO_CC:GO:0009507, chloroplast# (5e−41) 774 Putative glycerol3-phosphate permease n = 1 Tax = Zea mays RepID = Q7FS87_MAIZE (1e−139);GO_MF:GO:0003964, RNA-directed DNA polymerase, group II intron 8 74.791402660 91403709 encoded# (1e−139); GO_BP:GO:0055085, transmembranetransport# (1e−139); GO_CC:GO:0005634, nucleus# (1e−51) 775 Annexin-likeprotein RJ4 n = 1 Tax = Zea mays RepID = B6SUM2_MAIZE (1e−111); Annexin:Annexin (7.4e−20); Annexin: Annexin (3.3e−05); Annexin: Annexin (0.001);8 74.7 91421508 91423145 Annexin: Annexin (6.8e−21); GO_MF:GO:0005544,calcium-dependent phospholipid binding# (1e−118); GO_BP:GO:0009651,IEP#response to salt stress# (4e−58); GO_CC:GO:0005773, IDA#vacuole#(4e−58) 776 Putative uncharacterized protein n = 2 Tax = Zea mays RepID= B6TSZ8_MAIZE (2e−50) 8 74.7 91443937 91447409 777 Putativeuncharacterized protein n = 2 Tax = Zea mays RepID = B6TSZ8_MAIZE(2e−25) 8 74.7 91450931 91451101 778 CM0216.540.nc protein (Fragment) n= 1 Tax = Lotus japonicus RepID = B0BL99_LOTJA (3e−72) 8 74.7 9146789491483313 779 HAT family dimerisation domain containing protein n = 1 Tax= Oryza sativa Japonica Group RepID = Q2QP95_ORYSJ (8e−77); DUF659:Protein of unknown function (DUF 8 74.7 91476796 91479291 659)(4.7e−71); hATC: hAT family dimerisation domain (0.0008);GO_MF:GO:0046983, protein dimerization activity# (1e−102);GO_BP:GO:0006278, RNA-dependent DNA replication# (2e−79);GO_CC:GO:0005622, intracellular# (8e−87) 780 Glucosamine 6-phosphateN-acetyltransferase n = 2 Tax = Andropogoneae RepID = B6TIB6_MAIZE(1e−13); GO_MF:GO:0016740, transferase activity# (1e−13); 8 74.891498648 91501917 GO_BP:GO:0008152, metabolic process# (1e−13);GO_CC:GO:0016020, membrane# (5e−10) 781 Polcalcin Jun o 2 n = 2 Tax =Zea mays RepID = B6SGX1_MAIZE (4e−75); SPARC_Ca_bdg: Secreted proteinacidic and rich in cys (0.031); efhand: EF hand (5.6e−07); efhand: 874.8 91616373 91617428 EF hand (5.9e−07); efhand: EF hand (3.9e−06);efhand: EF hand (1.2e−06); GO_MF:GO:0005509, calcium ion storageactivity# (4e−75); GO_BP:GO:0055114, oxidation reduction# (4e−35);GO_CC:GO:0005737, cytoplasm# (6e−33) 782 Putative permease 1 n = 1 Tax =Oryza sativa Japonica Group RepID = Q6Z257_ORYSJ (0.0); Xan_ur_permease:Permease family (5.6e−55); GO_MF:GO:0005215, transporter 8 74.8 9167495391678496 activity# (0.0); GO_BP:GO:0055085, transmembrane transport#(0.0); GO_CC:GO:0016020, membrane# (0.0) 783 Protein aq_1857 n = 3 Tax =Andropogoneae RepID = B6T2K9_MAIZE (4e−80); Fe—S_biosyn: Iron-sulphurcluster biosynthesis (1.9e−21); GO_MF:GO:0051536, iron-sulfur 8 74.891681514 91689201 cluster binding# (4e−80); GO_BP:GO:0016226,iron-sulfur cluster assembly# (4e−80); GO_CC:GO:0005739, mitochondrion#(3e−38) 784 Ubiquinone biosynthesis protein ubiB n = 3 Tax =Andropogoneae RepID = B6UDS6_MAIZE (0.0); ABC1: ABC1 family (1.2e−36);APH: Phosphotransferase enzyme family 8 74.8 91775984 91779470 (0.0072);GO_MF:GO:0005524, ATP binding# (0.0); GO_BP:GO:0006468, protein aminoacid phosphorylation# (0.0); GO_CC:GO:0010287, IDA#plastoglobule# (0.0)785 Superoxide dismutase [Mn] 3.1, mitochondrial n = 9 Tax =Andropogoneae RepID = SODM1_MAIZE (2e−81); Sod_Fe_N: Iron/manganesesuperoxide dismutases, (2e−42); 8 74.8 91831542 91837848 Sod_Fe_C:Iron/manganese superoxide dismutases, C-term (3.7e−13);GO_MF:GO:0046872, metal ion binding# (2e−81); GO_BP:GO:0055114,oxidation reduction# (2e−81); GO_CC:GO:0005759, IEP#mitochondrialmatrix# (2e−81) 786 Superoxide dismutase [Mn] 3.1, mitochondrial n = 9Tax = Andropogoneae RepID = SODM1_MAIZE (6e−26); Sod_Fe_C:Iron/manganese superoxide dismutases, C-term (6.3e−09); 8 74.8 9184358891844328 GO_MF:GO:0046872, metal ion binding# (6e−26); GO_BP:GO:0055114,oxidation reduction# (6e−26); GO_CC:GO:0005759, IEP#mitochondrialmatrix# (6e−26) 787 Ovate protein n = 1 Tax = Solanum lycopersicum RepID= Q8GSM4_SOLLC (5e−14); DUF623: Protein of unknown function, DUF623(2.6e−31); GO_MF:GO:0016564, 8 74.8 91861669 91862649 transcriptionrepressor activity# (1e−16); GO_BP:GO:0016564, transcription repressoractivity# (1e−16); GO_CC:GO:0005856, cytoskeleton# (1e−16) 788 Coatomersubunit beta′-1 n = 4 Tax = BEP clade RepID = COB21_ORYSJ (1e−27);Coatomer_WDAD: Coatomer WD associated region (0.0034); GO_MF:GO:0005515,protein 8 74.8 91863984 91867269 binding# (1e−27); GO_BP:GO:0016192,vesicle-mediated transport# (1e−27); GO_CC:GO:0031410, IDA#cytoplasmicvesicle# (1e−27) 789 Integral membrane protein n = 2 Tax = AndropogoneaeRepID = B4F879_MAIZE (2e−50) 8 74.8 91867446 91868065 790 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B4FYW0_MAIZE(1e−36) 8 74.8 92026555 92027132 791 Basic helix-loop-helix protein-liken = 2 Tax = Oryza sativa RepID = Q5SMX5_ORYSJ (6e−21); HLH:Helix-loop-helix DNA-binding domain (5.5e−11); GO_MF:GO:0030528, 8 74.892199625 92200983 transcription regulator activity# (6e−21);GO_BP:GO:0045449, regulation of transcription# (6e−21);GO_CC:GO:0005634, nucleus# (6e−21) 792 Thioredoxin-like 6 n = 3 Tax =Zea mays RepID = B6TGT1_MAIZE (4e−96); Thioredoxin: Thioredoxin(1.9e−09); GO_MF:GO:0016671, oxidoreductase activity, acting on sulfur 874.8 92487005 92499851 group of donors, disulfide as acceptor# (2e−52);GO_BP:GO:0045454, cell redox homeostasis# (4e−96); GO_CC:GO:0031969,IDA#chloroplast membrane# (2e−52) 793 Putative uncharacterized protein n= 2 Tax = Zea mays RepID = B6TKW9_MAIZE (1e−105) 8 74.8 9269977192700945 794 Putative uncharacterized protein Sb10g022510 n = 1 Tax =Sorghum bicolor RepID = C5Z520_SORBI (4e−10) 8 74.8 92746543 92746869795 HAT family dimerisation domain containing protein n = 1 Tax = Oryzasativa Japonica Group RepID = Q2QP95_ORYSJ (9e−53); zf-C2HC_plant:Protein of unknown function, 8 74.8 92869580 92872255 DUF1544 (1.8e−12);DUF659: Protein of unknown function (DUF 659) (6.9e−35);GO_MF:GO:0046983, protein dimerization activity# (2e−76);GO_BP:GO:0015074, DNA integration# (1e−60); GO_CC:GO:0005622,intracellular# (1e−63) 796 Putative uncharacterized protein Sb07g028390n = 1 Tax = Sorghum bicolor RepID = C5YJ87_SORBI (1e−09) 8 74.8 9288489192885225 797 Carboxy-terminal domain RNA polymerase II polypeptide Asmall phosphatase, putative n = 1 Tax = Ricinus communis RepID =B9S8F4_RICCO (6e−53); NIF: NLI interacting 8 74.8 93132760 93134234factor-like phosphatase (4.7e−52); GO_MF:GO:0016791, phosphataseactivity# (2e−67); GO_BP:GO:0016791, phosphatase activity# (2e−67) 79860S ribosomal protein L17 n = 19 Tax = Poaceae RepID = RL17_MAIZE(3e−90); Ribosomal_L22: Ribosomal protein L22p/L17e (2.4e−76);GO_MF:GO:0003735, structural 8 74.8 93176442 93179884 constituent ofribosome# (3e−90); GO_BP:GO:0006412, translation# (3e−90);GO_CC:GO:0030529, ribonucleoprotein complex# (3e−90) 799 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6SQE3_MAIZE(3e−45) 8 74.8 93196973 93197774 800 Transposon protein, putative,CACTA, En/Spm sub-class n = 2 Tax = Oryza sativa RepID = Q7XGX1_ORYSJ(1e−17); GO_MF:GO:0004803, transposase activity# (1e−17); 8 74.893223318 93223659 GO_BP:GO:0006313, transposition, DNA-mediated# (1e−17)801 OSJNBa0089K21.9 protein n = 2 Tax = Oryza sativa RepID =Q7XQM7_ORYSJ (3e−52); Plant_tran: Plant transposon protein (0.017);GO_MF:GO:0004803, transposase activity# 8 74.8 93241056 93242205(7e−55); GO_BP:GO:0006313, transposition, DNA-mediated# (7e−55) 802P0696G06.8 protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q7F446_ORYSJ (4e−16); GO_MF:GO:0004803, transposase activity# (7e−20);GO_BP:GO:0006313, 8 74.8 93242275 93242629 transposition, DNA-mediated#(7e−20) 803 UNE1-like protein n = 1 Tax = Gossypioides kirkii RepID =B2ZAS0_9ROSI (1e−47); DUF641: Plant protein of unknown function (DUF641)(3.8e−47); GO_BP:GO:0009567, 8 74.8 93251576 93253289 IMP#doublefertilization forming a zygote and endosperm# (9e−47); GO_CC:GO:0005886,plasma membrane# (3e−37) 804 Fyve finger-containing phosphoinositidekinase, fyv1, putative n = 1 Tax = Ricinus communis RepID = B9RR30_RICCO(5e−30); GO_MF:GO:0016307, phosphatidylinositol 8 74.8 93468937 93469554phosphate kinase activity# (9e−41); GO_BP:GO:0046488,phosphatidylinositol metabolic process# (9e−41); GO_CC:GO:0005739,mitochondrion# (1e−26) 805 60S ribosomal protein L12 n = 1 Tax = Zeamays RepID = B6T1W9_MAIZE (5e−18); Ribosomal_L11_N: Ribosomal proteinL11, N-terminal domain (7.3e−07); 8 74.8 93482587 93484032GO_MF:GO:0003735, structural constituent of ribosome# (5e−18);GO_BP:GO:0006412, translation# (5e−18); GO_CC:GO:0030529,ribonucleoprotein complex# (5e−18) 806 Serine/threonine-specific proteinkinase-like protein n = 3 Tax = Glycine max RepID = C6ZRT4_SOYBN(1e−145); Pkinase: Protein kinase domain (5.5e−42); Pkinase_Tyr: Protein8 74.8 93489504 93495397 tyrosine kinase (6.4e−32); APH:Phosphotransferase enzyme family (0.023); GO_MF:GO:0016301, kinaseactivity# (1e−169); GO_BP:GO:0016301, kinase activity# (1e−169);GO_CC:GO:0005886, plasma membrane# (1e−140) 807 AT hook motif-containingprotein, putative n = 2 Tax = Oryza sativa Japonica Group RepID =Q2R0Z1_ORYSJ (3e−78); DUF889: Eukaryotic protein of unknown function 874.8 93539713 93544827 (DUF889) (9.6e−63); GO_MF:GO:0004386, helicaseactivity# (6e−78) 808 Mitochondrial 2-oxoglutarate/malate carrierprotein n = 5 Tax = Andropogoneae RepID = B6T8M6_MAIZE (2e−32);Mito_carr: Mitochondrial carrier protein (0.0017); 8 74.8 9356980293571933 GO_MF:GO:0005488, binding# (2e−32); GO_BP:GO:0055085,transmembrane transport# (2e−32); GO_CC:GO:0016021, integral tomembrane# (2e−32) 809 Mitochondrial 2-oxoglutarate/malate carrierprotein n = 5 Tax = Andropogoneae RepID = B6T8M6_MAIZE (5e−34);Mito_carr: Mitochondrial carrier protein (4.6e−15); 8 74.8 9357213193572687 GO_MF:GO:0005488, binding# (5e−34); GO_BP:GO:0055085,transmembrane transport# (5e−34); GO_CC:GO:0016021, integral tomembrane# (5e−34) 810 3-5 exonuclease, putative n = 1 Tax = Ricinuscommunis RepID = B9RFH0_RICCO (7e−95); GO_MF:GO:0008408, 3′-5′exonuclease activity# (1e−165); GO_BP:GO:0006139, 8 74.8 9367425993677433 nucleobase, nucleoside, nucleotide and nucleic acid metabolicprocess# (1e−165); GO_CC:GO:0005622, intracellular# (1e−165) 811Mitochondrial carrier protein, putative n = 1 Tax = Ricinus communisRepID = B9SAW7_RICCO (2e−98); Mito_carr: Mitochondrial carrier protein(2.4e−28); Mito_carr: 8 74.8 93795543 93801583 Mitochondrial carrierprotein (1.5e−15); Mito_carr: Mitochondrial carrier protein (2.6e−32);GO_MF:GO:0005488, binding# (1e−122); GO_BP:GO:0055085, transmembranetransport# (1e−122); GO_CC:GO:0016021, integral to membrane# (1e−122)812 Ubiquitin-protein ligase/zinc ion binding protein n = 1 Tax = Zeamays RepID = B6TFQ4_MAIZE (1e−23); GO_MF:GO:0016874, ligase activity#(1e−23); GO_BP:GO:0016567, 8 74.8 93883059 93884132 IGI#proteinubiquitination# (1e−23); GO_CC:GO:0005634, nucleus# (1e−23) 813 CsPK3 n= 1 Tax = Cucumis sativus RepID = Q9XGL2_CUCSA (2e−27); Pkinase: Proteinkinase domain (0.0073); GO_MF:GO:0005524, ATP binding# (1e−48); 8 74.893918308 93919077 GO_BP:GO:0006468, protein amino acid phosphorylation#(1e−48); GO_CC:GO:0005886, plasma membrane# (6e−22) 814Subtilisin-chymotrypsin inhibitor CI-1C n = 1 Tax = Zea mays RepID =B6SLR8_MAIZE (2e−33); potato_inhibit: Potato inhibitor I family (5e−29);GO_MF:GO:0004867, 8 74.8 93920061 93920505 chymotrypsin inhibitoractivity# (2e−33); GO_BP:GO:0009611, IEP#response to wounding# (2e−33);GO_CC:GO:0005576, extracellular region# (4e−09) 815 Putative polyproteinn = 1 Tax = Zea mays RepID = Q8SA93_MAIZE (5e−18); GO_MF:GO:0003964,RNA-directed DNA polymerase, group II intron encoded# (5e−18); 8 74.893932449 93932886 GO_BP:GO:0015074, DNA integration# (5e−18);GO_CC:GO:0005634, nucleus# (5e−18) 816 HAT family dimerisation domaincontaining protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QRD1_ORYSJ (2e−24); Herpes_UL3: Herpesvirus UL3 protein (0.065); 874.8 94180820 94181657 zf-BED: BED zinc finger (0.00035);GO_MF:GO:0046983, protein dimerization activity# (2e−24) 817Potassium-chloride cotransporter n = 3 Tax = Poaceae RepID =Q6Z0E2_ORYSJ (3e−18); GO_MF:GO:0015377, TAS#cation:chloride symporteractivity# (3e−18); 8 74.8 94299038 94299217 GO_BP:GO:0055085,transmembrane transport# (3e−18); GO_CC:GO:0016021, integral tomembrane# (3e−18) 818 Potassium-chloride cotransporter n = 3 Tax =Poaceae RepID = Q6Z0E2_ORYSJ (0.0); AA_permease: Amino acid permease(2e−08); DUF2074: Predicted permease (DUF2074) 8 74.8 94300293 94309786(0.028); GO_MF:GO:0015377, TAS#cation:chloride symporter activity#(0.0); GO_BP:GO:0055085, transmembrane transport# (0.0);GO_CC:GO:0016021, integral to membrane# (0.0) 819 Potassium-chloridecotransporter n = 3 Tax = Poaceae RepID = Q6Z0E2_ORYSJ (0.0);GO_MF:GO:0015377, TAS#cation:chloride symporter activity# (0.0); 8 74.894316729 94318509 GO_BP:GO:0055085, transmembrane transport# (0.0);GO_CC:GO:0016021, integral to membrane# (0.0) 820 60S ribosomal proteinL29 n = 9 Tax = Andropogoneae RepID = B6U0G5_MAIZE (2e−24);Ribosomal_L29e: Ribosomal L29e protein family (1.3e−17);GO_MF:GO:0003735, 8 74.8 94325768 94326837 structural constituent ofribosome# (2e−24); GO_BP:GO:0006412, translation# (2e−24);GO_CC:GO:0005840, ribosome# (2e−24) 821 Putative polyprotein n = 1 Tax =Zea mays RepID = Q8SA93_MAIZE (3e−35); GO_MF:GO:0003964, RNA-directedDNA polymerase, group II intron encoded# (3e−35); 8 74.8 9435335494353677 GO_BP:GO:0015074, DNA integration# (3e−35); GO_CC:GO:0005634,nucleus# (3e−35) 822 ATP binding protein n = 2 Tax = Andropogoneae RepID= B6TPY7_MAIZE (0.0); PPR: PPR repeat (1.3); PPR: PPR repeat (0.46);PPR: PPR repeat (0.0027); PPR: PPR repeat 8 74.8 94438246 94440138(5e−08); PPR: PPR repeat (5.4e−05); PPR: PPR repeat (8.6e−11); PPR: PPRrepeat (1.3e−07); PPR: PPR repeat (1.1e−06); PPR: PPR repeat (2.7); PPR:PPR repeat (1.1e−06); GO_MF:GO:0005488, binding# (1e−159);GO_BP:GO:0006350, transcription# (1e−159); GO_CC:GO:0005739,mitochondrion# (1e−142) 823 ATP binding protein n = 2 Tax =Andropogoneae RepID = B6TPY7_MAIZE (2e−19) 8 74.8 824 Catalytic/proteinphosphatase type 2C n = 2 Tax = Zea mays RepID = B6TWB0_MAIZE (1e−20);GO_MF:GO:0003824, catalytic activity# (1e−20); GO_BP:GO:0004721, 8 74.991968859 91969406 phosphoprotein phosphatase activity# (5e−17);GO_CC:GO:0005886, plasma membrane# (1e−11) 825 Enzyme inhibitor,putative n = 1 Tax = Ricinus communis RepID = B9RZP7_RICCO (2e−10);PMEI: Plant invertase/pectin methylesterase inhibitor (8.2e−09); 8 74.991992448 91993305 GO_MF:GO:0030599, pectinesterase activity# (1e−54);GO_BP:GO:0004857, enzyme inhibitor activity# (1e−54) 826 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B4FA23_MAIZE(8e−24) 8 74.9 92048358 92048663 827 Beta-1,3-galactosyltransferasesqv-2, putative n = 1 Tax = Ricinus communis RepID = B9RRS4_RICCO(2e−87); Galactosyl_T: Galactosyltransferase (1.4e−10); 8 74.9 9207817392082751 GO_MF:GO:0016757, transferase activity, transferring glycosylgroups# (1e−133); GO_BP:GO:0006486, protein amino acid glycosylation#(1e−133); GO_CC:GO:0016021, integral to membrane# (1e−133) 828 Putativereotransposon protein n = 1 Tax = Zea mays RepID = Q7XBD1_MAIZE (7e−09)8 74.9 92084158 92111298 829 Lachrymatory factor synthase n = 1 Tax =Zea mays RepID = B6TW34_MAIZE (3e−76); Polyketide_cyc2: Polyketidecyclase/dehydrase and li (3.6e−20); GO_CC:GO:0005773, 8 74.9 9303261993033449 IDA#vacuole# (3e−19) 830 Lustrin A-like n = 2 Tax = Oryzasativa RepID = Q8S237_ORYSJ (1e−127); DUF231: Arabidopsis proteins ofunknown function (3.6e−69) 8 75.2 94574619 94578897 831 Probablemetal-nicotianamine transporter YSL3 n = 1 Tax = Oryza sativa JaponicaGroup RepID = YSL3_ORYSJ (0.0); OPT: OPT oligopeptide transporterprotein (1.6e−42); 8 75.3 94591376 94597428 GO_BP:GO:0055085,transmembrane transport# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 832 Probable metal-nicotianamine transporter YSL3 n = 1 Tax =Oryza sativa Japonica Group RepID = YSL3_ORYSJ (0.0); OPT: OPToligopeptide transporter protein (1.7e−108); 8 75.3 94653380 94669018GO_BP:GO:0055085, transmembrane transport# (0.0); GO_CC:GO:0016021,integral to membrane# (0.0) 833 UPF0497 membrane protein BLE3 n = 2 Tax= Oryza sativa RepID = BLE3_ORYSJ (5e−45); DUF588: Domain of unknownfunction (DUF588) (1e−41); GO_BP:GO:0035264, 8 75.3 94697148 94698282IGI#multicellular organism growth# (5e−45); GO_CC:GO:0016021, integralto membrane# (5e−45) 834 Putative uncharacterized protein Sb09g008160 n= 1 Tax = Sorghum bicolor RepID = C5YV99_SORBI (7e−13);GO_MF:GO:0003779, actin binding# (4e−12); 8 75.3 94755448 94758478GO_BP:GO:0007010, cytoskeleton organization# (4e−12); GO_CC:GO:0015629,actin cytoskeleton# (4e−12) 835 Putative uncharacterized proteinSb09g008150 n = 1 Tax = Sorghum bicolor RepID = C5YV97_SORBI (1e−39);GO_MF:GO:0046872, metal ion binding# (3e−09) 8 75.3 94799124 94799840836 Triose phosphate/phosphate translocator n = 2 Tax = Zea mays RepID =B6T5Y2_MAIZE (4e−75); TPT: Triose-phosphate Transporter family (1e−41);DUF6: Integral membrane 8 75.3 94842243 94847299 protein DUF6 (0.013);GO_MF:GO:0005215, transporter activity# (4e−75); GO_BP:GO:0006810,transport# (4e−75); GO_CC:GO:0016021, integral to membrane# (3e−75) 837Triose phosphate/phosphate translocator n = 2 Tax = Zea mays RepID =B6T5Y2_MAIZE (1e−95); GO_MF:GO:0005215, transporter activity# (1e−95);GO_BP:GO:0006810, 8 75.3 94869786 94872368 transport# (1e−95);GO_CC:GO:0016021, integral to membrane# (1e−95) 838 Putativeretrotransposon protein n = 1 Tax = Phyllostachys edulis RepID =D3IVP0_9POAL (3e−56); GO_MF:GO:0004386, helicase activity# (1e−55) 875.3 94971334 94972115 839 Putative retrotransposon protein n = 1 Tax =Phyllostachys edulis RepID = D3IVP0_9POAL (2e−31); GO_MF:GO:0004386,helicase activity# (1e−30) 8 75.3 94973500 94974300 840 OSJNBa0095H06.12protein n = 1 Tax = Oryza sativa Japonica Group RepID = Q7XS07_ORYSJ(4e−17); DUF889: Eukaryotic protein of unknown function (DUF889)(0.0045); 8 75.3 94974339 94989426 GO_MF:GO:0004386, helicase activity#(2e−16) 841 Xylose isomerase n = 7 Tax = Poaceae RepID = Q8H3Q7_ORYSJ(1e−126); Coatomer_E: Coatomer epsilon subunit (2.1e−06); AP_endonuc_2:AP endonuclease family 2 (3.9e−11); 8 75.3 94993912 94998988GO_MF:GO:0046872, metal ion binding# (1e−126); GO_BP:GO:0042732,D-xylose metabolic process# (1e−126); GO_CC:GO:0005737, cytoplasm#(1e−126) 842 Putative uncharacterized protein n = 1 Tax = Zea mays RepID= B6SKJ1_MAIZE (2e−33) 8 75.3 95009577 95012351 843 DNA double-strandbreak repair rad50 ATPase, putative n = 1 Tax = Ricinus communis RepID =B9T5G8_RICCO (2e−70); Tropomyosin: Tropomyosin (0.0042); 8 75.3 9501294695017551 GO_MF:GO:0008565, protein transporter activity# (1e−142);GO_BP:GO:0008565, protein transporter activity# (1e−142);GO_CC:GO:0005737, cytoplasm# (1e−142) 844 DRE-binding protein 1c n = 2Tax = Zea mays RepID = C3UZ65_MAIZE (1e−142); AP2: AP2 domain (2.8e−20);GO_MF:GO:0003700, transcription factor activity# (1e−135); 8 75.395020011 95023360 GO_BP:GO:0045449, regulation of transcription#(1e−135); GO_CC:GO:0005634, nucleus# (1e−135) 845 Patellin-5 n = 2 Tax =Zea mays RepID = B6U0S4_MAIZE (0.0); CRAL_TRIO_N: CRAL/TRIO, N-terminus(2.9e−06); CRAL_TRIO: CRAL/TRIO domain (1.7e−19); 8 75.3 9512593195129886 GO_MF:GO:0008289, lipid binding# (1e−145); GO_BP:GO:0051301,cell division# (1e−145); GO_CC:GO:0016020, membrane# (1e−145) 846OSJNBa0079M09.12 protein n = 9 Tax = Oryza sativa Japonica Group RepID =Q7XVR0_ORYSJ (6e−17); Transposase_28: Putative gypsy type transposon(2.6e−08) 8 75.3 95306870 95307627 847 OSJNBa0079M09.12 protein n = 9Tax = Oryza sativa Japonica Group RepID = Q7XVR0_ORYSJ (7e−19);Transposase_28: Putative gypsy type transposon (1.4e−09) 8 75.3 9532821295328969 848 Endo beta n-acetylglucosaminidase, putative n = 1 Tax =Ricinus communis RepID = B9S465_RICCO (1e−33); GO_MF:GO:0033925,mannosyl-glycoprotein endo-beta-N- 8 75.3 95420532 95423294acetylglucosaminidase activity# (1e−33); GO_BP:GO:0006879, cellular ironion homeostasis# (2e−12); GO_CC:GO:0005737, cytoplasm# (1e−33) 849 Endobeta n-acetylglucosaminidase, putative n = 1 Tax = Ricinus communisRepID = B9S465_RICCO (1e−117); Glyco_hydro_85: Glycosyl hydrolase family85 (5e−112); 8 75.3 95433379 95436049 GO_MF:GO:0033925,mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity#(1e−123); GO_BP:GO:0008152, metabolic process# (9e−75);GO_CC:GO:0005737, cytoplasm# (1e−123) 850 Isoform 2 of Probablemetal-nicotianamine transporter YSL3 n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q6AVD0-2 (1e−30); GO_BP:GO:0055085, transmembrane 8 75.494610750 94611415 transport# (9e−31); GO_CC:GO:0016021, integral tomembrane# (1e−30) 851 Putative uncharacterized protein n = 1 Tax = Zeamays RepID = B6U8H0_MAIZE (4e−13) 8 75.4 94612455 94612974 852ATP-dependent RNA helicase, putative n = 1 Tax = Ricinus communis RepID= B9R8Y0_RICCO (3e−21); GO_MF:GO:0016787, hydrolase activity# (1e−22); 875.4 94624909 94636392 GO_BP:GO:0008380, RNA splicing# (2e−20);GO_CC:GO:0005829, IDA#cytosol# (2e−20) 853 Putative uncharacterizedprotein n = 1 Tax = Zea mays RepID = B8A0N1_MAIZE (3e−19) 8 75.495481303 95481716 854 Retrotransposon protein, putative, unclassified n= 1 Tax = Oryza sativa Japonica Group RepID = Q2QSH2_ORYSJ (3e−39);GO_MF:GO:0004523, ribonuclease H activity# (3e−39); 8 75.4 9556904295569679 GO_BP:GO:0006278, RNA-dependent DNA replication# (3e−39) 855Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6TZX3_MAIZE (7e−30) 8 75.4 95573013 95573303 856 Aldose reductase n = 3Tax = Andropogoneae RepID = B6THE1_MAIZE (1e−170); Aldo_ket_red:Aldo/keto reductase family (1.2e−129); GO_MF:GO:0016491, oxidoreductase8 75.5 95631071 95638909 activity# (1e−170); GO_BP:GO:0055114, oxidationreduction# (1e−170); GO_CC:GO:0005829, IDA#cytosol# (1e−110) 857Putative uncharacterized protein Sb09g022330 n = 3 Tax = AndropogoneaeRepID = C5YZ73_SORBI (5e−15) 8 75.6 95641896 95643652 858 Os05g0455600 n= 1 Tax = Oryza sativa Japonica Group RepID = UPI0000E1250C (1e−106);PRA1: PRA1 family protein (3.9e−43); GO_BP:GO:001692, vesicle-mediated 875.65 95646214 95650515 transport# (3e−76); GO_CC:GO:0016021, integralto membrane# (3e−76) 859 Delta-1-pyrroline-5-carboxylase synthetase 2 n= 2 Tax = Andropogoneae RepID = C8CB72_SORBI (0.0); AA_kinase: Aminoacid kinase family (2.4e−55); 8 75.9 95715355 95726173 Aldedh: Aldehydedehydrogenase family (0.00019); GO_MF:GO:0016740, transferase activity#(0.0); GO_BP:GO:0055114, oxidation reduction# (0.0); GO_CC:GO:0005737,cytoplasm# (0.0) 860 Nucleic acid binding protein, putative n = 1 Tax =Ricinus communis RepID = B9RRU4_RICCO (1e−28); KH_1: KH domain(1.5e−10); 8 75.9 95735166 95743294 GO_MF:GO:0003723, RNA binding#(1e−52); GO_BP:GO:0006396, RNA processing# (1e−22) 861 Putativetranscription factor qSH-1 n = 1 Tax = Oryza rufipogon RepID =A9XWR4_ORYRU (1e−131); POX: Associated with HOX (1.3e−48); Homeobox: 875.9 95774436 95779361 Homeobox domain (0.0015); GO_MF:GO:0043565,sequence-specific DNA binding# (0.0); GO_BP:GO:0045449, regulation oftranscription# (0.0); GO_CC:GO:0005634, nucleus# (0.0) 862 Putativeuncharacterized protein Sb03g001220 n = 1 Tax = Sorghum bicolor RepID =C5XKB1_SORBI (1e−10); GO_MF:GO:0005525, GTP binding# (3e−10); 8 7696058925 96059534 GO_CC:GO:0005622, intracellular# (3e−10) 863Pollen-specific kinase partner protein n = 2 Tax = Zea mays RepID =B6U1X9_MAIZE (0.0); PRONE: PRONE (Plant-specific Rop nucleotide exc(3.8e−223); 8 76 96075884 96079303 GO_MF:GO:0016301, kinase activity#(0.0); GO_BP:GO:0016301, kinase activity# (0.0); GO_CC:GO:0016324,IDA#apical plasma membrane# (1e−132) 864 OSJNBa0033G05.13 protein n = 1Tax = Oryza sativa Japonica Group RepID = Q7XTM9_ORYSJ (1e−176);GO_MF:GO:0008270, zinc ion binding# (1e−176); 8 76 96178451 96180104GO_BP:GO:0015074, DNA integration# (1e−176); GO_CC:GO:0005622,intracellular# (1e−171) 865 Integrase core domain containing protein n =1 Tax = Oryza sativa Japonica Group RepID = Q75HA9_ORYSJ (1e−38);GO_MF:GO:0008270, zinc ion 8 76.2 96193386 96194276 binding# (1e−38);GO_BP:GO:0015074, DNA integration# (1e−38); GO_CC:GO:0005622,intracellular# (1e−35) 866 Probable anion transporter 2, chloroplastic n= 1 Tax = Oryza sativa Japonica Group RepID = PHT42_ORYSJ (0.0); MFS_1:Major Facilitator Superfamily (5.6e−20); 8 76.45 97844871 97849365Sugar_tr: Sugar (and other) transporter (0.063); GO_MF:GO:0005315,inorganic phosphate transmembrane transporter activity# (1e−131);GO_BP:GO:0055085, transmembrane transport# (0.0); GO_CC:GO:0031969,IDA#chloroplast membrane# (0.0) 867 Putative uncharacterized proteinSb09g020830 n = 1 Tax = Sorghum bicolor RepID = C5YY75_SORBI (9e−25) 876.55 102657595 102657840 868 Peroxidase 1 n = 3 Tax = Oryza sativaRepID = PER1_ORYSJ (1e−140); peroxidase: Peroxidase (2e−122);GO_MF:GO:0046872, metal ion binding# (1e−140); 8 76.9 96253851 96255187GO_BP:GO:0055114, oxidation reduction# (1e−140); GO_CC:GO:0005576,extracellular region# (1e−140) 869 Pleckstrin homology domain-containingprotein 1 n = 2 Tax = Andropogoneae RepID = B6T5A7_MAIZE (1e−92); PH: PHdomain (3.5e−24); GO_MF:GO:0008289, 8 76.9 96346829 96347799 lipidbinding# (2e−50); GO_BP:GO:0016301, kinase activity# (3e−10);GO_CC:GO:0005737, cytoplasm# (2e−50) 870 Putative uncharacterizedprotein Sb09g022010 n = 1 Tax = Sorghum bicolor RepID = C5YYU9_SORBI(1e−160); TPR_2: Tetratricopeptide repeat (2.6); TPR_2: 8 76.9 9663024996632523 Tetratricopeptide repeat (1.3); GO_MF:GO:0005488, binding#(1e−145); GO_BP:GO:0006396, RNA processing# (2e−29); GO_CC:GO:0005622,intracellular# (2e−29) 871 GHMP kinase-like protein n = 3 Tax = PoaceaeRepID = Q6YX79_ORYSJ (8e−62); Scramblase: Scramblase (3.8e−13);GO_MF:GO:0016773, phosphotransferase 8 77 96265286 96268048 activity,alcohol group as acceptor# (8e−62); GO_BP:GO:0016310,hyperphosphorylation# (8e−62); GO_CC:GO:0005737, cytoplasm# (8e−62) 872Emp24/gp25L/p24-like n = 1 Tax = Oryza sativa Japonica Group RepID =Q6ZGK3_ORYSJ (4e−16); GO_BP:GO:0006810, transport# (4e−18); 8 7796636446 96637406 GO_CC:GO:0016021, integral to membrane# (4e−18) 873Putative hydroxycinnamoyl transferase n = 2 Tax = Oryza sativa RepID =Q5N7V3_ORYSJ (1e−178); Transferase: Transferase family (9.4e−84); 8 7796819655 96821745 GO_MF:GO:006747, transferase activity, transferringacyl groups other than amino-acyl groups# (0.0) 874Coronatine-insensitive protein 1 n = 3 Tax = Andropogoneae RepID =B6TPN4_MAIZE (0.0); LRR_2: Leucine Rich Repeat (10); LRR_1: Leucine RichRepeat (20); 8 77 96929607 96933269 LRR_2: Leucine Rich Repeat (1.5);LRR_1: Leucine Rich Repeat (2.3e+02); LRR_1: Leucine Rich Repeat (13);LRR_1: Leucine Rich Repeat (1.7e+02); LRR_2: Leucine Rich Repeat (2.3);LRR_1: Leucine Rich Repeat (25); GO_MF:GO:0005515, protein binding#(1e−154); GO_BP:GO:0050832, IMP#defense response to fungus# (1e−154);GO_CC:GO:0019005, NAS#SCF ubiquitin ligase complex# (1e−154) 875AT_hook: AT hook motif (1); AT_hook: AT hook motif (1); AT_hook: AT hookmotif (1); AT_hook: AT hook motif (7); AT_hook: AT hook motif (11) 8 7796935835 96950132 876 MYB-like transcription factor DIVARICATA n = 2 Tax= Zea mays RepID = B6T0L0_MAIZE (1e−34); GO_MF:GO:0003677, DNA binding#(1e−34); 8 77 97042807 97043351 GO_BP:GO:0045449, regulation oftranscription# (1e−34); GO_CC:GO:0005634, nucleus# (1e−34) 877 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6SUB9_MAIZE(1e−100) 8 77 97055611 97057187 878 Putative uncharacterized protein n =2 Tax = Zea mays RepID = B6TGN0_MAIZE (1e−117) 8 77.1 96268745 96271064879 Transcription factor, putative n = 1 Tax = Ricinus communis RepID =B9RYX9_RICCO (7e−55); B3: B3 DNA binding domain (1.6e−29);GO_MF:GO:0003677, 8 77.1 96690364 96690912 DNA binding# (2e−61);GO_BP:GO:0045449, regulation of transcription# (2e−61);GO_CC:GO:0005634, nucleus# (2e−61) 880 ER glycerol-phosphateacyltransferase n = 1 Tax = Ricinus communis RepID = B9S2F2_RICCO(1e−121); Acyltransferase: Acyltransferase (9.6e−10); 8 77.1 9669259896698185 GO_MF:GO:0008415, acyltransferase activity# (0.0);GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0016021, integralto membrane# (1e−135) 881 Ulp1 protease family, C-terminal catalyticdomain containing protein n = 2 Tax = Oryza sativa Japonica Group RepID= Q109R5_ORYSJ (4e−33); Peptidase_C48: 8 77.1 96985721 96994606 Ulp1protease family, C-terminal catalytic domain (7.4e−09);GO_MF:GO:0008234, cysteine-type peptidase activity# (1e−138);GO_BP:GO:0006508, proteolysis# (1e−138) 882 Ulp1 protease family,C-terminal catalytic domain containing protein n = 2 Tax = Oryza sativaJaponica Group RepID = Q109R5_ORYSJ (2e−14); 8 77.1 96994621 96996396GO_MF:GO:0008234, cysteine- type peptidase activity# (2e−14);GO_BP:GO:0006508, proteolysis# (2e−14) 883 OSJNBa0036B21.17 protein n =6 Tax = Oryza sativa RepID = Q7XQ05_ORYSJ (6e−13) 8 77.1 9699759696998401 884 Putative uncharacterized protein Sb06g030295 n = 1 Tax =Sorghum bicolor RepID = C5Y8Y8_SORBI (2e−30) 8 77.1 96998426 96998719885 Transposon protein, putative, Pong sub-class n = 1 Tax = Oryzasativa Japonica Group RepID = Q7XDX1_ORYSJ (2e−10); GO_MF:GO:0003677, 877.1 97031270 97031829 DNA binding# (4e−10); GO_CC:GO:0005840, ribosome#(4e−10) 886 Putative uncharacterized protein Sb09g022000 n = 1 Tax =Sorghum bicolor RepID = C5YYU8_SORBI (5e−97) 8 77.15 96350550 96361050887 Putative PolI-like DNA polymerase n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q69S01_ORYSJ (3e−28); GO_MF:GO:0008408, 3′-5′ exonuclease8 77.15 96401217 96403741 activity# (3e−29); GO_BP:GO:0006260, DNAreplication# (3e−29); GO_CC:GO:0005622, intracellular# (3e−29) 888Galactosyltransferase family n = 3 Tax = Andropogoneae RepID =B6SXL2_MAIZE (1e−12); GO_MF:GO:0016757, transferase activity,transferring glycosyl 8 77.2 97117274 97117771 groups# (1e−12);GO_BP:GO:0006486, protein amino acid glycosylation# (1e−12);GO_CC:GO:0016021, integral to membrane# (1e−12) 889 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = C4J8U8_MAIZE(3e−91) 8 77.3 97176485 97177444 890 Putative uncharacterized proteinSb09g022160 n = 2 Tax = Sorghum bicolor RepID = C5YYW5_SORBI (5e−18);PAR1: PAR1 protein (0.0063) 8 77.3 97422405 97422741 891OSJNBa0067K08.12 protein n = 3 Tax = Oryza sativa RepID = Q7XUK2_ORYSJ(0.0); LETM1: LETM1-like protein (9.2e−157); GO_MF:GO:0005509, 8 77.397466729 97477275 calcium ion storage activity# (0.0); GO_BP:GO:0042407,cristae formation# (4e−76); GO_CC:GO:0016021, integral to membrane#(0.0) 892 HAT family dimerisation domain containing protein n = 1 Tax =Oryza sativa Japonica Group RepID = Q7XD60_ORYSJ (2e−67);GO_MF:GO:0046983, protein 8 77.3 97649960 97651201 dimerizationactivity# (2e−67); GO_BP:GO:0006468, protein amino acid phosphorylation#(5e−47) 893 Putative uncharacterized protein n = 1 Tax = Zea mays RepID= B4FKU7_MAIZE (1e−118) 8 77.4 97552114 97555230 894 Putative axi 1 n =2 Tax = Oryza sativa RepID = Q5N7B2_ORYSJ (0.0); DUF246: Plant proteinfamily (7.1e−186) 8 77.4 97712162 97716261 895 Putative uncharacterizedprotein n = 1 Tax = Oryza sativa Indica Group RepID = A2Y596_ORYSI(1e−167); DUF740: Protein of unknown function (DUF740) 8 77.4 9793965197941696 (4.3e−256); GO_CC:GO:0009536, plastid# (6e−14) 896Hexaprenyldihydroxybenzoate methyltransferase, putative n = 1 Tax =Ricinus communis RepID = B9S8D8_RICCO (1e−26); GO_MF:GO:0008425,2-polyprenyl- 8 77.4 97992716 97994155 6-methoxy-1,4-benzoquinonemethyltransferase activity# (1e−36); GO_BP:GO:0008152, metabolicprocess# (1e−36); GO_CC:GO:0005759, IEP#mitochondrial matrix# (8e−27)897 Putative dihydroxypolyprenylbenzoate methyltransferase n = 1 Tax =Oryza sativa Japonica Group RepID = Q5VMJ1_ORYSJ (1e−77);Methyltransf_11: 8 77.4 98026316 98029412 Methyltransferase domain(0.0027); GO_MF:GO:0008425, 2-polyprenyl-6-methoxy-1,4-benzoquinonemethyltransferase activity# (9e−78); GO_BP:GO:0008152, metabolicprocess# (9e−78); GO_CC:GO:0005759, IEP#mitochondrial matrix# (3e−69)898 Dehydrin: Dehydrin (2.4e−36) 8 77.4 98030042 98031216 899Pentatricopeptide repeat-containing protein, putative n = 1 Tax =Ricinus communis RepID = B9S789_RICCO (1e−153); PPR: PPR repeat (0.72);PPR: PPR 8 77.4 98076480 98079155 repeat (5.6e−09); PPR: PPR repeat(3.2e−09); PPR: PPR repeat (2.8e−13); PPR: PPR repeat (1.3e−12); PPR:PPR repeat (5.5e−09); PPR: PPR repeat (8e−09); PPR: PPR repeat(8.2e−15); PPR: PPR repeat (3.7e−08); PPR: PPR repeat (2.6e−08); PPR:PPR repeat (7.3e−13); PPR: PPR repeat (0.22); PPR: PPR repeat (0.019);PPR: PPR repeat (8.9e−10); PPR: PPR repeat (0.58); PPR: PPR repeat(0.34); GO_MF:GO:0016740, transferase activity# (2e−87);GO_BP:GO:0006278, RNA-dependent DNA replication# (2e−76);GO_CC:GO:0005739, mitochondrion# (9e−77) 900Lipopolysaccharide-modifying protein n = 1 Tax = Zea mays RepID =B6TEG9_MAIZE (1e−12); DUF821: Arabidopsis thaliana protein of unknownfunction 8 77.4 98080366 98080632 (DUF821) (0.0036) 901Arginyl-tRNA--protein transferase, putative n = 1 Tax = Ricinus communisRepID = B9SLM9_RICCO (4e−09); GO_MF:GO:0004057, arginyltransferase 877.5 98400172 98401261 activity# (8e−15); GO_BP:GO:0016598, proteinarginylation# (8e−15) 902 Arginyl-tRNA--protein transferase, putative n= 1 Tax = Ricinus communis RepID = B9SLM9_RICCO (1e−135); ATE_C:Arginine-tRNA-protein transferase, 8 77.5 98423247 98426294 C terminus(3.1e−70); GO_MF:GO:0030246, carbohydrate binding# (0.0);GO_BP:GO:0016598, protein arginylation# (0.0); GO_CC:GO:0005737,cytoplasm# (1e−48) 903 60S acidic ribosomal protein P2B n = 4 Tax =Andropogoneae RepID = RLA2B_MAIZE (2e−25); Ribosomal 60s: 60s Acidicribosomal protein (2.3e−32); 8 77.5 98594876 98599278 GO_MF:GO:0003735,structural constituent of ribosome# (2e−25); GO_BP:GO:0006414,translational elongation# (2e−25); GO_CC:GO:0030529, ribonucleoproteincomplex# (2e−25) 904 Pentatricopeptide repeat-containing protein,putative n = 1 Tax = Ricinus communis RepID = B9SCR2_RICCO (1e−129);PPR: PPR repeat (2.4e−07); PPR: PPR 8 77.5 98655289 98656905 repeat(0.0094); PPR: PPR repeat (1.4); PPR: PPR repeat (5.4e−10); PPR: PPRrepeat (4.2e−07); PPR: PPR repeat (8.5e−07); GO_MF:GO:0005488, binding#(4e−69); GO_CC:GO:0005739, mitochondrion# (1e−58) 905 Putativeuncharacterized protein n = 2 Tax = Zea mays RepID = Q5GAU8_MAIZE(2e−12) 8 77.5 98705541 98705880 906 Putative uncharacterized protein n= 1 Tax = Zea mays RepID = B4FJ19_MAIZE (1e−30) 8 77.5 98706736 98707101907 Aluminum-activated malate transporter-like n = 1 Tax = Oryza sativaJaponica Group RepID = Q5Z6M5_QRYSJ (1e−58); GO_MF:GO:0005253, IDA#anionchannel 8 77.6 98754050 98754826 activity# (9e−39); GO_BP:GO:0010044,response to aluminum ion# (1e−58); GO_CC:GO:0009705, IDA#plant-typevacuole membrane# (9e−39) 908 Zinc finger protein n = 1 Tax = Zea maysRepID = B6TXE6_MAIZE (8e−66); zf-C2H2: Zinc finger, C2H2 type (0.0059);zf-C2H2: Zinc finger, C2H2 type (0.4); 8 77.6 98765962 98768759 zf-C2H2:Zinc finger, C2H2 type (6.9); GO_MF:GO:0008270, zinc ion binding#(2e−93); GO_BP:GO:0045449, regulation of transcription# (2e−68);GO_CC:GO:0005622, intracellular# (2e−93) 909 Ferredoxin-6 n = 1 Tax =Zea mays RepID = B6STB1_MAIZE (9e−66); Fer2: 2Fe—2S iron-sulfur clusterbinding do (1.4e−28); GO_MF:GO:0051537, 2 iron, 8 77.6 99001628 990023742 sulfur cluster binding# (9e−66); GO_BP:GO:0022900, electron transportchain# (9e−66); GO_CC:GO:0009536, plastid# (2e−51) 910 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B4FA60 MAIZE(2e−21) 8 77.6 99072549 99073184 911 Glucan endo-1,3-beta-glucosidase 7n = 2 Tax = Zea mays RepID = B6SUM3_MAIZE (0.0); Glyco_hydro_17:Glycosyl hydrolases family 17 (8.7e−111); 8 77.6 99158642 99162373GO_MF:GO:0043169, cation binding# (0.0); GO_BP:GO:0008152, metabolicprocess# (0.0); GO_CC:GO:0046658, anchored to plasma membrane# (1e−118)912 Protein kinase-like n = 1 Tax = Oryza sativa Japonica Group RepID =Q6KAK1_ORYSJ (1e−111); GO_MF:GO:0016301, kinase activity# (1e−111); 877.6 99165877 99167516 GO_BP:GO:0016301, kinase activity# (1e−111) 913Transposase n = 1 Tax = Zea mays RepID = A5X2G8_MAIZE (5e−37); hATC: hATfamily dimerisation domain (1.5e−24); GO_MF:GO:0046983, protein 8 77.699194162 99194911 dimerization activity# (5e−37); GO_BP:GO:0032196,transposition# (5e−14) 914 Vesicle coat complex COPII, subunitSEC24/subunit SFB2 (ISS) n = 1 Tax = Ostreococcus tauri RepID =Q013K2_OSTTA (8e−39); zf-Sec23_Sec24: Sec23/Sec24 8 77.6 9920082799207530 zinc finger (0.00013); Sec23_trunk: Sec23/Sec24 trunk domain(1.7e−10); Sec23_BS: Sec23/Sec24 beta-sandwich domain (0.012);Sec23_helical: Sec23/Sec24 helical domain (0.00076); GO_MF:GO:0008270,zinc ion binding# (0.0); GO_BP:GO:0006888, ER to Golgi vesicle-mediatedtransport# (0.0); GO_CC:GO:0030127, COPII vesicle coat# (0.0) 915Putative uncharacterized protein n = 1 Tax = Zea mays RepID =B6TT12_MAIZE (3e−13) 8 77.6 99224994 99225706 916 Tubulin bindingcofactor C n = 1 Tax = Medicago truncatula RepID = A2Q4U5_MEDTR (9e−10);GO_MF:GO:0005488, binding# (4e−21) 8 77.6 99226248 99229285 917 NACtranscription factor-like protein n = 2 Tax = Oryza sativa RepID =Q94CW0_ORYSJ (3e−62); NAM: No apical meristem (NAM) protein (8e−39); 877.6 99283158 99284291 GO_MF:GO:0003677, DNA binding# (5e−75);GO_BP:GO:0045449, regulation of transcription# (5e−75);GO_CC:GO:0005634, nucleus# (5e−48) 918 Retrotransposon protein,putative, unclassified n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QM33_ORYSJ (4e−11); GO_MF:GO:0003676, nucleic acid 8 77.6 9941792299422033 binding# (4e−11) 919 Putative uncharacterized proteinOSJNBa0026O12.1 n = 2 Tax = Oryza sativa RepID = Q9AUZ8_ORYSJ (2e−10);GO_MF:GO:0005509, calcium ion storage 8 77.6 99513812 99514817 activity#(2e−10) 920 Putative retroelement n = 1 Tax = Zea mays RepID =Q7XBD5_MAIZE (7e−49); GO_MF:GO:0003917, DNA topoisomerase type Iactivity# (7e−49); 8 77.6 99519614 99523100 GO_BP:GO:0006268, DNAunwinding factor# (7e−49); GO_CC:GO:0005694, chromosome# (7e−49) 921(RAP Annotation release2) Histone deacetylase superfamily protein n = 1Tax = Oryza sativa Japonica Group RepID = B7ETM3_ORYSJ (3e−85); NC: NCdomain 8 77.6 99590456 99592590 (7.8e−06); GO_BP:GO:0006370, mRNAcapping# (1e−44) 922 (RAP Annotation release2) Histone deacetylasesuperfamily protein n = 3 Tax = Oryza sativa RepID = Q60DG7_ORYSJ (0.0);Hist_deacetyl: Histone deacetylase 8 77.6 99593881 99597132 family(4.4e−112); GO_MF:GO:0016787, hydrolase activity# (0.0);GO_BP:GO:0045449, regulation of transcription# (1e−144);GO_CC:GO:0005634, nucleus# (1e−144) 923 B1160F02.10 protein n = 1 Tax =Oryza sativa Japonica Group RepID = Q6MWG6_ORYSJ (6e−44); hATC: hATfamily dimerisation domain (7.7e−29); 8 77.6 100258896 100259536GO_MF:GO:0046983, protein dimerization activity# (1e−66);GO_BP:GO:0055114, oxidation reduction# (4e−21) 924 Actin-11 n = 9 Tax =Viridiplantae RepID = ACT11_ARATH (0.0); Actin: Actin (2e−237);GO_MF:GO:0005524, ATP binding# (0.0); GO_BP:GO:0014866, 8 77.6 100394524100398658 IMP#skeletal myofibril assembly# (0.0); GO_CC:GO:0005886,plasma membrane# (0.0) 925 Catalytic, putative n = 1 Tax = Ricinuscommunis RepID = B9T4I0_RICCO (2e−29); GO_MF:GO:0051536, iron-sulfurcluster binding# (3e−34); 8 77.6 100419432 100419995 GO_BP:GO:0006364,rRNA processing# (3e−34); GO_CC:GO:0005737, cytoplasm# (3e−34) 926Catalytic, putative n = 1 Tax = Ricinus communis RepID = B9T4I0_RICCO(9e−13); GO_MF:GO:0051536, iron-sulfur cluster binding# (7e−29); 8 77.6100427857 100428441 GO_BP:GO:0006364, rRNA processing# (7e−29);GO_CC:GO:0005737, cytoplasm# (7e−29) 927 Putative gag/pol polyprotein n= 1 Tax = Oryza sativa Japonica Group RepID = Q75IA0_ORYSJ (8e−68);RVT_2: Reverse transcriptase (RNA-dependent DNA 8 77.6 100469457100470771 pol (1.4e−13); GO_MF:GO:0003677, DNA binding# (8e−68);GO_BP:GO:0015074, DNA integration# (8e−68) 928 (RAP Annotation release2)Histone deacetylase superfamily protein n = 1 Tax = Oryza sativaJaponica Group RepID = B7ETM3_ORYSJ (6e−85); NC: NC 8 77.6 100472235100472981 domain (7.8e−06); GO_BP:GO:0006370, mRNA capping# (7e−45) 929(RAP Annotation release2) Histone deacetylase superfamily protein n = 3Tax = Oryza sativa RepID = Q60DG7_ORYSJ (1e−131); Hist_deacetyl: Histonedeacetylase 8 77.6 100473591 100478046 family (6.6e−68);GO_MF:GO:0016787, hydrolase activity# (1e−155); GO_BP:GO:0045449,regulation of transcription# (1e−103); GO_CC:GO:0005634, nucleus#(1e−103) 930 Putative uncharacterized protein Sb09g021760 n = 3 Tax =Andropogoneae RepID = C5YYS1_SORBI (7e−11) 8 77.6 100479211 100479579931 Putative retroelement n = 1 Tax = Zea mays RepID = Q7XBD5_MAIZE(3e−85); GO_MF:GO:0003917, DNA topoisomerase type I activity# (3e−85); 877.6 100591797 100595283 GO_BP:GO:0006268, DNA unwinding factor#(3e−85); GO_CC:GO:0005694, chromosome# (3e−85) 932 Retrotransposonprotein, putative, unclassified n = 1 Tax = Oryza sativa Japonica GroupRepID = Q2QM33_ORYSJ (2e−10); GO_MF:GO:0003676, nucleic acid 8 77.6100598062 100599636 binding# (2e−10) 933 Retrotransposon protein,putative, unclassified n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QQG4_ORYSJ (5e−18); GO_MF:GO:0003964, 8 77.6 100618292 100618468RNA-directed DNA polymerase, group II intron encoded# (5e−18);GO_BP:GO:0006278, RNA-dependent DNA replication# (5e−18) 934 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B6TBA7_MAIZE(3e−17) 8 77.6 100625551 100626262 935 Putative uncharacterized proteinn = 1 Tax = Zea mays RepID = C4J932_MAIZE (4e−18); GO_MF:GO:0048038,quinone binding# (4e−18); GO_BP:GO:0055114, 8 77.6 100626320 100630111oxidation reduction# (4e−18); GO_CC:GO:0005777, IDA#peroxisome# (7e−11)936 OSJNBa0055H05.12 protein n = 2 Tax = Oryza sativa Japonica GroupRepID = Q7XRD0_ORYSJ (1e−23); PHD: PHD-finger (0.00093);GO_MF:GO:0046872, 8 77.6 100630313 100630562 metal ion binding# (1e−23)937 G-box-binding factor 4 n = 1 Tax = Zea mays RepID = B6TGZ0_MAIZE(7e−83); bZIP_1: bZIP transcription factor (7.7e−08); bZIP_2: Basicregion leucine 8 77.6 100710645 100714109 zipper (3.1e−07);GO_MF:GO:0046983, protein dimerization activity# (7e−83);GO_BP:GO:0006355, regulation of transcription, DNA-dependent# (7e−83);GO_CC:GO:0005634, nucleus# (7e−83) 938 Jacalin-like lectin domaincontaining protein n = 1 Tax = Oryza sativa Japonica Group RepID =Q2R1E0_ORYSJ (2e−73); Pkinase: Protein kinase domain (7.1e−36); 8 77.6100755519 100757762 Pkinase_Tyr: Protein tyrosine kinase (8.1e−17);Jacalin: Jacalin-like lectin domain (4.7e−17); GO_MF:GO:0005524, ATPbinding# (1e−73); GO_BP:GO:0006468, protein amino acid phosphorylation#(1e−73); GO_CC:GO:0016021, integral to membrane# (5e−41) 939Retrotransposon gag protein n = 1 Tax = Asparagus officinalis RepID =Q2AA53_ASPOF (7e−32); 3_5_exonuc: 3′-5′ exonuclease (1.3e−06);Retrotrans_gag: 8 77.7 97344781 97349148 Retrotransposon gag protein(5.6e−09); GO_MF:GO:0004523, ribonuclease H activity# (3e−29);GO_BP:GO:0015074, DNA integration# (3e−29); GO_CC:GO:0005634, nucleus#(3e−29) 940 Class III peroxidase 124 n = 3 Tax = Oryza sativa RepID =Q5U1G9_ORYSJ (1e−111); peroxidase: Peroxidase (2.4e−106);GO_MF:GO:0046872, metal ion 8 77.8 102488572 102490148 binding#(1e−111); GO_BP:GO:0055114, oxidation reduction# (1e−111);GO_CC:GO:0005886, plasma membrane# (1e−77) 941 Putative uncharacterizedprotein Sb03g010270 n = 3 Tax = Andropogoneae RepID = C5XGH5_SORBI(0.0); GO_MF:GO:0003964, RNA-directed DNA 8 77.95 102398429 102405402polymerase, group II intron encoded# (3e−63); GO_BP:GO:0006278,RNA-dependent DNA replication# (3e−63) 942 Putative Pol polyprotein fromtransposon element Bs1 n = 1 Tax = Zea mays RepID = POLB_MAIZE (1e−22);GO_MF:GO:0016887, ATPase activity# (1e−22); 8 78 100121781 100122131GO_BP:GO:0016820, hydrolase activity, acting on acid anhydrides,catalyzing transmembrane movement of substances# (1e−22);GO_CC:GO:0016021, integral to membrane# (1e−22) 943 Protein argonaute 18n = 2 Tax = Oryza sativa RepID = AGO18_ORYSJ (9e−42); Piwi: Piwi domain(1.5e−08); GO_MF:GO:0003676, nucleic acid binding#(9e−42); 8 78102382112 102383707 GO_BP:GO:0031047, IMP#gene silencing by RNA#(9e−42); GO_CC:GO:0005737, cytoplasm# (1e−34) 944 RNA recognitionmotif-containing protein n = 2 Tax = Zea mays RepID = B6SM38_MAIZE(6e−11); GO_MF:GO:0003676, nucleic acid binding# (8e−10) 8 78.2102262836 102264592 945 Fructose-1,6-bisphosphatase 2 n = 3 Tax =Poaceae RepID = A7J2C3_ORYSJ (1e−125); FBPase: Fructose1-6-bisphosphatase (4.1e−92); GO_MF:GO:0042578, 8 78.3 100871386100874570 phosphoric ester hydrolase activity# (1e−125);GO_BP:GO:0042132, fructose 1,6-bisphosphate 1-phosphatase activity#(1e−125); GO_CC:GO:0005737, cytoplasm# (1e−125) 946 Putativepolypyrimidine track-binding protein n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q5TKN2_ORYSJ (0.0); RRM_1: RNA recognition motif. 8 78.3100935130 100939953 (a.k.a. RRM, RB (0.039); RRM_1: RNA recognitionmotif. (a.k.a. RRM, RB (1.9e−07); RRM_1: RNA recognition motif. (a.k.a.RRM, RB (3.9e−06); GO_MF:GO:0003723, RNA binding# (0.0);GO_BP:GO:0006397, mRNA processing# (0.0); GO_CC:GO:0005634, nucleus#(0.0) 947 COV1-like protein n = 4 Tax = Andropogoneae RepID =B6SMB8_MAIZE (1e−130); DUF502: Protein of unknown function (DUF502)(3.7e−35) 8 78.3 100957806 100964937 948 Serine/arginine rich splicingfactor, putative n = 1 Tax = Ricinus communis RepID = B9T013_RICCO(4e−09); GO_MF:GO:0003676, nucleic acid binding# (7e−20) 8 78.4101025715 101026655 949 Beta-galactosidase n = 1 Tax = Oryza sativaIndica Group RepID = B2Z6M9_ORYSI (0.0); Glyco_hydro_35: Glycosylhydrolases family 35 (3.7e−155); 8 78.4 102164879 102171452Glyco_hydro_42: Beta- galactosidase (0.063); Glyco_hydro_2_N: Glycosylhydrolases family 2, sugar binding domain (0.096); GO_MF:GO:0043169,cation binding# (0.0); GO_BP:GO:0008152, metabolic process# (0.0);GO_CC:GO:0048046, IDA#apoplast# (0.0) 950 Retrotransposon protein n = 1Tax = Zea mays RepID = B6U894_MAIZE (1e−99); GO_MF:GO:0004386, helicaseactivity# (2e−35) 8 78.5 101141978 101143455 951 Potassium channelprotein ZMK2 n = 1 Tax = Zea mays RepID = Q9SM12_MAIZE (0.0); Ion_trans:Ion transport protein (1.7e−21); Ion_trans_2: Ion channel 8 78.5102104140 102108633 (2.4e−16); cNMP_binding: Cyclic nucleotide-bindingdomain (5.1e−15); Ank: Ankyrin repeat (2.8e−08); Ank: Ankyrin repeat(1.1); Ank: Ankyrin repeat (0.00058); GO_MF:GO:0005249, voltage-gatedpotassium channel activity# (0.0); GO_BP:GO:0055085, transmembranetransport# (0.0); GO_CC:GO:0016021, integral to membrane# (0.0) 952Universal stress protein family protein n = 2 Tax = Zea mays RepID =B6TUC5_MAIZE (1e−86); Usp: Universal stress protein family (6.9e−11); 878.5 102135988 102138900 GO_BP:GO:0006950, response to stress# (1e−86)953 BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, putativen = 1 Tax = Ricinus communis RepID = B9RDW7_RICCO (1e−141); Pkinase_Tyr:8 78.6 101176449 101179599 Protein tyrosine kinase (2.3e−28); Pkinase:Protein kinase domain (4.3e−36); GO_MF:GO:0005524, ATP binding# (0.0);GO_BP:GO:0006468, protein amino acid phosphorylation# (0.0);GO_CC:GO:0016021, integral to membrane# (1e−141) 954 Potassium channelprotein ZMK2 n = 1 Tax = Zea mays RepID = Q9SM12_MAIZE (6e−49);GO_MF:GO:0005249, voltage-gated potassium channel activity# 8 78.6102073137 102074215 (6e−49); GO_BP:GO:0055085, transmembrane transport#(6e−49); GO_CC:GO:0016021, integral to membrane# (6e−49) 955 Cucumisin,putative n = 1 Tax = Ricinus communis RepID = B9R7A2_RICCO (0.0);Inhibitor_I9: Peptidase inhibitor I9 (9.8e−16); Peptidase_S8: Subtilasefamily 8 78.7 101208361 101211017 (1e−10); PA: PA domain (0.0019);GO_MF:GO:0043086, negative regulation of catalytic activity# (0.0);GO_BP:GO:0043086, negative regulation of catalytic activity# (0.0);GO_CC:GO:0009505, IDA#expansin# (1e−124) 956 Putative polyprotein n = 1Tax = Oryza sativa Japonica Group RepID = Q60DG5_ORYSJ (2e−20);GO_MF:GO:0008270, zinc ion binding# (2e−20); 8 78.7 101230093 101231548GO_BP:GO:0015074, DNA integration# (2e−20); GO_CC:GO:0005634, nucleus#(2e−17) 957 Beta-hydroxyacyl-ACP dehydratase n = 3 Tax = AndropogoneaeRepID = B6TG22_MAIZE (1e−120); FabA: FabA-like domain (2.7e−53);GO_MF:GO:0016836, 8 78.7 101237227 101240359 hydro-lyase activity#(1e−120); GO_BP:GO:0009245, lipid A biosynthetic process# (1e−120);GO_CC:GO:0005737, cytoplasm# (1e−120) 958 Cyclin-A1-1 n = 3 Tax = Oryzasativa RepID = CCA11_ORYSJ (9e−44); Cyclin_N: Cyclin, N-terminal domain(1.1e−31); GO_BP:GO:0051301, cell division# 8 78.8 101261002 101265381(9e−44); GO_CC:GO:0005634, nucleus# (9e−44) 959 Putative uncharacterizedprotein Sb09g021460 n = 2 Tax = Andropogoneae RepID = C5YYN8_SORBI(1e−40) 8 78.8 101266458 101267208 960 Putative uncharacterized proteinn = 1 Tax = Zea mays RepID = B6UH53_MAIZE (2e−86); DUF1218: Protein ofunknown function (DUF1218) (2e−27) 8 78.8 101274727 101275883 961Putative uncharacterized protein Sb09g021230 n = 3 Tax = AndropogoneaeRepID = C5YYC2_SORBI (1e−51) 8 78.9 101958089 101962101 962 Coiled-coildomain-containing protein 47 n = 2 Tax = Xenopus RepID = CCD47_XENLA(1e−30); DUF1682: Protein of unknown function (DUF1682) (2.2e−127); 8 79101384099 101387219 GO_MF:GO:0005515, protein binding# (3e−30);GO_BP:GO:0055074, calcium ion homeostasis# (3e−30); GO_CC:GO:0016021,integral to membrane# (1e−115) 963 Aspartate aminotransferase n = 3 Tax= Andropogoneae RepID = B6TK79_MAIZE (0.0); Aminotran_1_2:Aminotransferase class I and II (2.4e−80); 8 79 101389828 101413491Beta_elim_lyase: Beta-eliminating lyase (0.037); GO_MF:GO:0030170,pyridoxal phosphate binding# (0.0); GO_BP:GO:0016847,1-aminocyclopropane-1-carboxylate synthase activity# (0.0);GO_CC:GO:0009507, chloroplast# (1e−171) 964 Acetyltransferase, GNATfamily protein n = 2 Tax = Andropogoneae RepID = B6UHR7_MAIZE (7e−74);Acetyltransf_1: Acetyltransferase (GNAT) family 8 79 101417576 101419017(3.6e−05); GO_MF:GO:0016740, transferase activity# (7e−74);GO_BP:GO:0008152, metabolic process# (7e−74) 965 OSJNBa0028I23.15protein n = 1 Tax = Oryza sativa Japonica Group RepID = Q7XMG7_ORYSJ(1e−40); GO_MF:GO:0003964, RNA-directed DNA polymerase, 8 79 101424956101425932 group II intron encoded# (1e−40); GO_BP:GO:0006278,RNA-dependent DNA replication# (1e−40); GO_CC:GO:0016020, membrane#(1e−10) 966 Cytosolic factor-like protein n = 2 Tax = Oryza sativa RepID= Q8RYZ1_ORYSJ (3e−67); GO_MF:GO:0005215, transporter activity# (9e−47);8 79 101899837 101900533 GO_BP:GO:0006810, transport#(9e−47);GO_CC:GO:0005622, intracellular# (9e−47) 967 Peptide transporter PTR2-Bn = 2 Tax = Zea mays RepID = B6SWT0_MAIZE (0.0); MFS_1: MajorFacilitator Superfamily (4.6e−05); Sugar_tr: Sugar (and other) 8 79.05101425993 101431760 transporter (0.066); PTR2: POT family (8.1e−93);GO_MF:GO:0005215, transporter activity# (0.0); GO_BP:GO:0006857,oligopeptide transport# (0.0); GO_CC:GO:0016021, integral to membrane#(0.0) 968 Asparagine synthetase [glutamine-hydrolyzing] n = 1 Tax = Zeamays RepID = ASNS_MAIZE (2e−16); GO_MF:GO:0016874, ligase activity#(2e−16); 8 79.1 101433486 101436392 GO_BP:GO:0008652, cellular aminoacid biosynthetic process# (2e−16) 969 Amidophosphoribosyltransferase n= 2 Tax = Andropogoneae RepID = B6SRU6_MAIZE (0.0); GATase_2: Glutamineamidotransferases class-II (3.3e−34); 8 79.1 101438279 101440231Pribosyltran: Phosphoribosyl transferase domain (8.2e−17);GO_MF:GO:0016757, transferase activity, transferring glycosyl groups#(0.0); GO_BP:GO:0009116, nucleoside metabolic process# (0.0);GO_CC:GO:0005618, IDA#cell wall# (1e−174) 970 Retrotransposon protein,putative, unclassified n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QQR5_ORYSJ (5e−30); zf-CCHC: Zinc knuckle (0.0083); 8 79.1 101476251101478825 GO_MF:GO:0008270, zinc ion binding# (2e−35); GO_BP:GO:0006278,RNA-dependent DNA replication# (5e−30) 971 Hydrolase, putative n = 1 Tax= Ricinus communis RepID = B9S718_RICCO (1e−67); DLH: Dienelactonehydrolase family (8e−10); Peptidase_S9: Prolyl 8 79.1 101872864101874328 oligopeptidase family (0.053); Abhydrolase_4: TAP-like protein(0.062); GO_MF:GO:0016787, hydrolase activity# (1e−67) 972Gibberellin-regulated protein 2 n = 3 Tax = Andropogoneae RepID =B6SKV6_MAIZE (6e−32); GASA: Gibberellin regulated protein (4.5e−37); 879.2 101507754 101508326 GO_MF:GO:0005515, protein binding# (1e−16);GO_BP:GO:0009826, IMP#unidimensional cell growth# (1e−16);GO_CC:GO:0009505, IDA#expansin# (1e−16) 973 Myb-like DNA-binding domaincontaining protein n = 2 Tax = Andropogoneae RepID = B4G152_MAIZE(6e−32); GO_MF:GO:0003677, DNA binding# (1e−34); 8 79.2 101817191101818525 GO_BP:GO:0045449, regulation of transcription# (1e−34);GO_CC:GO:0005634, nucleus# (1e−34) 974 Putative polyprotein n = 1 Tax =Oryza sativa Japonica Group RepID = Q75LZ2_ORYSJ (4e−40);GO_MF:GO:0003964, RNA-directed DNA polymerase, group II 8 79.2 101819090101819454 intron encoded# (4e−40); GO_BP:GO:0015074, DNA integration#(4e−40); GO_CC:GO:0005634, nucleus# (3e−39) 975 Retrotransposon protein,putative, unclassified n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QTY9_ORYSJ (3e−77); GO_MF:GO:0004190, penicillopepsin 8 79.2 101819470101820719 activity# (8e−84); GO_BP:GO:0015074, DNA integration# (8e−84);GO_CC:GO:0005634, nucleus# (8e−84) 976 ATP binding protein n = 1 Tax =Zea mays RepID = B6U6Y9_MAIZE (0.0); dNK: Deoxynucleoside kinase(1.4e−25); GO_MF:GO:0016773, phosphotransferase 8 79.2 101822359101828755 activity, alcohol group as acceptor# (0.0); GO_BP:GO:0006139,nucleobase, nucleoside, nucleotide and nucleic acid metabolic process#(0.0); GO_CC:GO:0005634, nucleus# (1e−172) 977 Transposon protein,putative, CACTA, En/Spm sub-class n = 1 Tax = Oryza sativa JaponicaGroup RepID = Q2R337_ORYSJ (3e−18); GO_MF:GO:0004803, 8 79.3 101749333101749489 transposase activity# (2e−17); GO_BP:GO:0006313,transposition, DNA-mediated# (2e−17) 978 Transposon protein, putative,CACTA, En/Spm sub-class n = 1 Tax = Oryza sativa Japonica Group RepID =Q2QWY8_ORYSJ (2e−46); GO_MF:GO:0004803, 8 79.3 101749553 101750213transposase activity# (2e−29); GO_BP:GO:0006313, transposition,DNA-mediated# (2e−29) 979 Putative uncharacterized protein Sb01g018120 n= 1 Tax = Sorghum bicolor RepID = C5WX97_SORBI (1e−17) 8 79.3 101751450101752438 980 Phosphoribosylanthranilate transferase n = 2 Tax =Andropogoneae RepID = B6UEE3_MAIZE (0.0); C2: C2 domain (2.1e−15); C2:C2 domain (2e−24); 8 79.3 101754270 101757247 PRT_C: Plantphosphoribosyltransferase C-ter (1.2e−121); GO_MF:GO:0016740,transferase activity# (0.0); GO_CC:GO:0009507, chloroplast# (0.0) 981Myb transcription factor n = 3 Tax = Oryza sativa RepID = Q5TKI8_ORYSJ(3e−60); Myb_DNA-binding: Myb-like DNA-binding domain (5e−11); 8 79.3101774593 101775500 Myb_DNA-binding: Myb-like DNA-binding domain(2.3e−05); GO_MF:GO:0003677, DNA binding# (2e−95); GO_BP:GO:0045449,regulation of transcription# (2e−95); GO_CC:GO:0005634, nucleus# (2e−95)982 Clathrin assembly protein, putative n = 1 Tax = Ricinus communisRepID = B9SCP6_RICCO (1e−134); ANTH: ANTH domain (1.3e−92); ENTH: ENTH 879.35 102531812 102534027 domain (0.019); GO_MF:GO:0030276, clathrinbinding# (0.0); GO_BP:GO:0048268, IDA#clathrin coat assembly# (0.0);GO_CC:GO:0030118, clathrin coat# (0.0) 983 OSJNBa0088K19.7 protein n = 3Tax = Oryza sativa RepID = Q7XUZ4_ORYSJ (1e−105); DUF668: Protein ofunknown function (DUF668) (1.5e−47); 8 79.5 101670238 101675867GO_MF:GO:0016301, kinase activity# (1e−105); GO_BP:GO:0016301, kinaseactivity# (1e−105); GO_CC:GO:0005886, plasma membrane# (1e−169) 984Hexaprenyldihydroxybenzoate methyltransferase, putative n = 1 Tax =Ricinus communis RepID = B9S8D8_RICCO (2e−22); GO_MF:GO:0008425,2-polyprenyl- 8 80.8 97281684 97282429 6-methoxy-1,4-benzoquinonemethyltransferase activity# (8e−27); GO_BP:GO:0008152, metabolicprocess# (8e−27); GO_CC:GO:0005759, IEP#mitochondrial matrix# (5e−22)985 Pentatricopeptide (PPR) repeat-containing protein-like n = 9 Tax =Oryza RepID = Q69XF8_ORYSJ (4e−82); PPR: PPR repeat (0.011); PPR: PPRrepeat (9.4e−11); 8 80.9 102573183 102573785 PPR: PPR repeat (5.1);GO_MF:GO:0005488, binding# (6e−43); GO_BP:GO:0055085, transmembranetransport# (6e−41); GO_CC:GO:0016020, membrane# (6e−41) 986 60Sribosomal protein L36 n = 3 Tax = Zea mays RepID = B6TRR4_MAIZE (1e−23);Ribosomal_L36e: Ribosomal protein L36e (3.7e−14); GO_MF:GO:0003735, 8 81116533029 116534824 structural constituent of ribosome# (1e−23);GO_BP:GO:0006412, translation# (1e−23); GO_CC:GO:0030529,ribonucleoprotein complex# (1e−23) 987 Nodulin-like protein n = 1 Tax =Zea mays RepID = B6U1N6_MAIZE (0.0); Caa3_CtaG: Cytochrome c oxidasecaa3 assembly fa (0.069); DUF6: Integral 8 81.05 97286995 97289328membrane protein DUF6 (2.6e−09); TPT: Triose-phosphate Transporterfamily (0.024); DUF6: Integral membrane protein DUF6 (0.0099);GO_CC:GO:0016020, membrane# (0.0) 988 26S proteasome non-ATPaseregulatory subunit 8 n = 4 Tax = Andropogoneae RepID = B4FCY9_MAIZE(4e−44); GO_MF:GO:0005488, binding# (1e−15); 8 81.1 115892831 115894124GO_BP:GO:0006508, proteolysis# (4e−44); GO_CC:GO:0005838, IDA#proteasomeregulatory particle# (4e−44) 989 Putative uncharacterized protein n = 2Tax = Zea mays RepID = B4FK11_MAIZE (1e−144); zf-C3HC4: Zinc finger,C3HC4 type (RING finger) (0.00067); 8 81.1 115904453 115906293GO_MF:GO:0046872, metal ion binding# (1e−144) 990 Putativeuncharacterized protein Sb09g022420 n = 3 Tax = Andropogoneae RepID =C5YZ83_SORBI (0.0); TPR_2: Tetratricopeptide repeat (2.5); TPR_2: 8 81.1116022174 116026953 Tetratricopeptide repeat (8); TPR_2:Tetratricopeptide repeat (0.28); GO_MF:GO:0005488, binding# (1e−69) 991Heat shock cognate 70 kDa protein n = 7 Tax = Magnoliophyta RepID =HSP7C_PETHY (0.0); HSP70: Hsp70 protein (0); MreB_Mbl: MreB/Mbl protein(0.0035); 8 81.1 116617617 116621838 Hydantoinase_A:Hydantoinase/oxoprolinase (0.1); GO_MF:GO:0005524, ATP binding# (0.0);GO_BP:GO:0006950, response to stress# (0.0); GO_CC:GO:0048046,IDA#apoplast# (0.0) 992 Cysteinyl-tRNA synthetase n = 2 Tax = Zea maysRepID = B6SH65_MAIZE (0.0); tRNA-synt_1e: tRNA synthetases class I (C)(3e−164); tRNA-synt_1g: tRNA 8 81.1 116620898 116625760 synthetasesclass I (M) (6.5e−05); GO_MF:GO:0005524, ATP binding# (0.0);GO_BP:GO:0006423, cysteinyl-tRNA aminoacylation# (0.0);GO_CC:GO:0005737, cytoplasm# (0.0) 993 Glycerol-3-phosphateacyltransferase 8 n = 3 Tax = Andropogoneae RepID = B6SWK2_MAIZE(1e−174); Acyltransferase: Acyltransferase (2.4e−06); 8 81.15 116118395116120825 GO_MF:GO:0008415, acyltransferase activity# (0.0);GO_BP:GO:0008152, metabolic process# (0.0); GO_CC:GO:0016021, integralto membrane# (1e−118) 994 Vrga1 n = 1 Tax = Aegilops ventricosa RepID =Q9SED7_AEGVE (2e−92); NB-ARC: NB-ARC domain (6e−56); NACHT: NACHT domain(0.042); 8 81.2 104379532 104382266 GO_MF:GO:0005524, ATP binding#(0.0); GO_BP:GO:0006952, defense response# (0.0) 995 Putativeuncharacterized protein n = 1 Tax = Zea mays RepID = B4FBY9_MAIZE(4e−12) 8 81.2 115828221 115831895 996 Putative uncharacterized proteinSb07g019100 n = 1 Tax = Sorghum bicolor RepID = C5YKI4_SORBI (1e−125);hATC: hAT family dimerisation domain (0.00027); 8 81.25 116199837116201263 GO_MF:GO:0046983, protein dimerization activity# (7e−53);GO_BP:GO:0015074, DNA integration# (5e−46); GO_CC:GO:0005622,intracellular# (3e−47) 997 Putative growth regulator n = 4 Tax =Malvoideae RepID = B2ZAU8_GOSAR (9e−88); DUF246: Plant protein family(3.9e−198); GO_CC:GO:0005794, 8 81.35 116425630 116442282 IDA#Golgiapparatus# (7e−87) 998 Putative uncharacterized protein Sb01g035540 n =1 Tax = Sorghum bicolor RepID = C5X0M0_SORBI (5e−12) 8 81.4 115779956115780594 999 Importin beta 1 n = 3 Tax = Oryza sativa RepID =Q9ZWR5_ORYSJ (0.0); IBN_N: Importin-beta N-terminal domain (1e−23);HEAT: HEAT repeat (5.5); 8 81.4 115785480 115791621 HEAT: HEAT repeat(0.013); HEAT: HEAT repeat (0.62); HEAT: HEAT repeat (0.025); HEAT: HEATrepeat (31); GO_MF:GO:0008565, protein transporter activity# (0.0);GO_BP:GO:0008565, protein transporter activity# (0.0); GO_CC:GO:0009507,chloroplast# (0.0) 1000 Flavonoid 3-monooxygenase n = 2 Tax = Zea maysRepID = B6TML3_MAIZE (0.0); p450: Cytochrome P450 (2.9e−93);GO_MF:GO:0046872, metal ion 8 81.4 116203880 116209001 binding# (0.0);GO_BP:GO:0055114, oxidation reduction# (0.0); GO_CC:GO:0016021, integralto membrane# (1e−143) 1001 Osmotic avoidance abnormal protein, putativen = 1 Tax = Ricinus communis RepID = B9T259_RICCO (1e−159); Kinesin:Kinesin motor domain (1.2e−122); 8 81.45 116420366 116424771 HHH:Helix-hairpin-helix motif (0.024); GO_MF:GO:0005524, ATP binding# (0.0);GO_BP:GO:0007018, microtubule-based movement# (0.0); GO_CC:GO:0005874,microtubule# (0.0) 1002 Putative MURAZC n = 1 Tax = Zea mays RepID =Q8H6I1_MAIZE (7e−41); GO_MF:GO:0008270, zinc ion binding# (2e−43);GO_BP:GO:0006313, 8 81.6 116399389 116399814 transposition,DNA-mediated# (2e−43) ^(†)cM = centiMorgans; ^(††)bp = base pair ofArizona Genomics Institute B73 RefGen_v2 sequence.

What is claimed is:
 1. A method of obtaining a corn plant with enhancedFusarium stalk rot resistance comprising: a) providing a population ofcorn plants; b) detecting in said plants the presence of a Fusariumstalk rot resistance allele at a polymorphic locus genetically linked toa chromosomal segment flanked by: marker loci psk2 and gpm753d; ormarker loci TIDP3099 and IDP4363; and c) selecting from said populationat least a first plant comprising said allele and enhanced Fusariumstalk rot resistance compared to a plant lacking said allele.
 2. Themethod of claim 1, wherein said segment is flanked by: marker lociTIDP3078 and umc60; or marker loci TIDP3728 and TIDP5537.
 3. The methodof claim 1, wherein said segment is flanked by: marker loci SEQ ID NO: 1and SEQ ID NO: 5; or marker loci csu329 and IDP6942.
 4. The method ofclaim 1, wherein said segment is flanked by: marker loci TIDP6282 andSEQ ID NO: 4; or marker loci SEQ ID NO: 90 and SEQ ID NO: 2; or markerloci SEQ ID NO: 6 and SEQ ID NO:
 17. 5. The method of claim 1, whereinsaid polymorphic locus comprises a nucleic acid sequence selected fromthe group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ IDNO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ IDNO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17; SEQ ID NO: 86,SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO:91, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ IDNO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, andSEQ ID NO:
 101. 6. The method of claim 1, further defined as comprisingselecting from said population at least two plants, thereby forming apopulation of corn plants comprising said allele and enhanced Fusariumstalk rot resistance compared to a plant lacking said allele.
 7. Themethod of claim 1, wherein said Fusarium stalk rot resistance allele wasintrogressed into said population of corn plants from a starting plantor population of corn plants containing said allele.
 8. The method ofclaim 1, further comprising producing a progeny plant with Fusariumstalk rot resistance from said first plant.
 9. The method of claim 8,wherein producing the progeny plant comprises marker-assisted selectionfor Fusarium stalk rot resistance.
 10. The method of claim 8, whereinthe progeny plant is an F2-F6 progeny plant.
 11. The method of claim 8,wherein producing the progeny plant comprises backcrossing.
 12. A methodof producing a corn plant with enhanced Fusarium stalk rot resistancecomprising: a) crossing a first corn plant comprising a Fusarium stalkrot resistance allele with a second corn plant of a different genotypeto produce one or more progeny plants; and b) selecting a progeny plantbased on the presence of said allele at a polymorphic locus geneticallylinked to a chromosomal segment flanked by: marker loci psk2 andgpm753d; or marker loci TIDP3099 and IDP4363; and wherein said alleleconfers enhanced resistance to Fusarium stalk rot compared to a plantlacking said allele.
 13. The method of claim 12, wherein said segment isflanked by: marker loci TIDP3078 and umc60; or marker loci TIDP3728 andTIDP5537.
 14. The method of claim 12, wherein said segment is flankedby: marker loci SEQ ID NO: 1 and SEQ ID NO: 5; or marker loci csu329 andIDP6942.
 15. The method of claim 12, wherein said segment is flanked by:marker loci TIDP6282 and SEQ ID NO: 4; or marker loci SEQ ID NO: 90 andSEQ ID NO: 2; or marker loci SEQ ID NO: 6 and SEQ ID NO:
 17. 16. Themethod of claim 12, wherein said polymorphic locus comprises a nucleicacid sequence selected from the group consisting of SEQ ID NO: 1, SEQ IDNO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ IDNO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ IDNO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQID NO: 17; SEQ ID NO: 86, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89,SEQ ID NO: 90, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO:94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ IDNO: 99, SEQ ID NO: 100, and SEQ ID NO:
 101. 17. The method of claim 12,wherein the progeny plant is an F2-F6 progeny plant.
 18. The method ofclaim 12, wherein producing the progeny plant comprises backcrossing.19. The method of claim 18, wherein backcrossing comprises from 2-7generations of backcrosses.
 20. The method of claim 18, whereinbackcrossing comprises marker-assisted selection in at least twogenerations.
 21. The method of claim 20, wherein backcrossing comprisesmarker-assisted selection in all generations.
 22. The method of claim12, wherein the first corn plant is an inbred or a hybrid.
 23. Themethod of claim 12, wherein the second corn plant is an agronomicallyelite corn plant.
 24. The method of claim 23, wherein the agronomicallyelite corn plant is an inbred or a hybrid.